Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3775

Experiment: N2E2wt with N1B4m denitrifying repB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3773 and Pf1N1B4_3774 are separated by 77 nucleotidesPf1N1B4_3774 and Pf1N1B4_3775 are separated by 75 nucleotidesPf1N1B4_3775 and Pf1N1B4_3776 are separated by 131 nucleotidesPf1N1B4_3776 and Pf1N1B4_3777 are separated by 238 nucleotides Pf1N1B4_3773 - GTP cyclohydrolase I (EC 3.5.4.16) type 1, at 4,183,521 to 4,184,066 _3773 Pf1N1B4_3774 - Smr domain protein, at 4,184,144 to 4,184,701 _3774 Pf1N1B4_3775 - FIG00956876: hypothetical protein, at 4,184,777 to 4,185,097 _3775 Pf1N1B4_3776 - Isochorismatase (EC 3.3.2.1), at 4,185,229 to 4,185,819 _3776 Pf1N1B4_3777 - Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p, at 4,186,058 to 4,186,966 _3777 Position (kb) 4184 4185 4186Strain fitness (log2 ratio) -3 -2 -1 0 1at 4184.187 kb on + strandat 4184.331 kb on - strand, within Pf1N1B4_3774at 4184.331 kb on - strand, within Pf1N1B4_3774at 4184.872 kb on - strand, within Pf1N1B4_3775at 4184.872 kb on - strand, within Pf1N1B4_3775at 4184.872 kb on - strand, within Pf1N1B4_3775at 4185.893 kb on + strandat 4185.893 kb on + strandat 4185.894 kb on - strandat 4185.947 kb on - strandat 4185.947 kb on - strandat 4185.963 kb on - strandat 4185.973 kb on + strandat 4185.973 kb on + strandat 4185.974 kb on - strandat 4185.974 kb on - strandat 4185.974 kb on - strandat 4186.023 kb on + strandat 4186.094 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
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4,184,187 + -2.1
4,184,331 - Pf1N1B4_3774 0.34 +1.1
4,184,331 - Pf1N1B4_3774 0.34 +0.7
4,184,872 - Pf1N1B4_3775 0.30 +0.7
4,184,872 - Pf1N1B4_3775 0.30 -1.8
4,184,872 - Pf1N1B4_3775 0.30 -1.6
4,185,893 + +0.7
4,185,893 + +0.1
4,185,894 - -0.4
4,185,947 - -2.9
4,185,947 - -3.1
4,185,963 - -1.2
4,185,973 + -2.8
4,185,973 + +0.7
4,185,974 - +0.7
4,185,974 - +1.5
4,185,974 - -0.7
4,186,023 + -1.0
4,186,094 - -1.6

Or see this region's nucleotide sequence