Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_375 and Pf1N1B4_376 are separated by 250 nucleotides Pf1N1B4_376 and Pf1N1B4_377 are separated by 211 nucleotides Pf1N1B4_377 and Pf1N1B4_378 are separated by 5 nucleotides Pf1N1B4_378 and Pf1N1B4_379 are separated by 153 nucleotides
Pf1N1B4_375 - Cyanophycin synthase (EC 6.3.2.29)(EC 6.3.2.30), at 445,407 to 447,152
_375
Pf1N1B4_376 - Deblocking aminopeptidase (EC 3.4.11.-) @ Cyanophycinase 2 (EC 3.4.15.6), at 447,403 to 448,593
_376
Pf1N1B4_377 - FIG00953242: hypothetical protein, at 448,805 to 449,032
_377
Pf1N1B4_378 - Carbon storage regulator, at 449,038 to 449,223
_378
Pf1N1B4_379 - Protein of unknown function UPF0060, at 449,377 to 449,709
_379
Position (kb) 447
448
449 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 446.557 kb on + strand, within Pf1N1B4_375 at 446.557 kb on + strand at 446.557 kb on + strand, within Pf1N1B4_375 at 446.557 kb on + strand, within Pf1N1B4_375 at 446.558 kb on - strand, within Pf1N1B4_375 at 446.558 kb on - strand, within Pf1N1B4_375 at 446.847 kb on + strand at 446.847 kb on + strand, within Pf1N1B4_375 at 446.847 kb on + strand, within Pf1N1B4_375 at 446.902 kb on + strand, within Pf1N1B4_375 at 446.902 kb on + strand, within Pf1N1B4_375 at 446.903 kb on - strand, within Pf1N1B4_375 at 446.990 kb on + strand at 447.042 kb on + strand at 447.043 kb on - strand at 447.135 kb on - strand at 447.436 kb on + strand at 447.436 kb on + strand at 447.437 kb on - strand at 447.437 kb on - strand at 447.753 kb on - strand, within Pf1N1B4_376 at 447.753 kb on - strand, within Pf1N1B4_376 at 448.216 kb on + strand, within Pf1N1B4_376 at 448.222 kb on + strand, within Pf1N1B4_376 at 448.298 kb on - strand, within Pf1N1B4_376 at 448.298 kb on - strand, within Pf1N1B4_376 at 448.298 kb on - strand, within Pf1N1B4_376 at 448.298 kb on - strand, within Pf1N1B4_376 at 448.438 kb on + strand, within Pf1N1B4_376 at 448.438 kb on + strand, within Pf1N1B4_376 at 448.439 kb on - strand, within Pf1N1B4_376 at 448.439 kb on - strand, within Pf1N1B4_376 at 448.439 kb on - strand, within Pf1N1B4_376 at 448.439 kb on - strand, within Pf1N1B4_376 at 448.747 kb on - strand at 448.762 kb on - strand at 448.804 kb on + strand at 448.804 kb on + strand at 449.082 kb on - strand, within Pf1N1B4_378 at 449.082 kb on - strand, within Pf1N1B4_378 at 449.082 kb on - strand, within Pf1N1B4_378 at 449.082 kb on - strand, within Pf1N1B4_378 at 449.098 kb on + strand, within Pf1N1B4_378 at 449.098 kb on + strand, within Pf1N1B4_378 at 449.098 kb on + strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.099 kb on - strand, within Pf1N1B4_378 at 449.216 kb on - strand at 449.312 kb on - strand at 449.312 kb on - strand at 449.358 kb on + strand at 449.359 kb on - strand at 449.510 kb on + strand, within Pf1N1B4_379 at 449.510 kb on + strand, within Pf1N1B4_379 at 449.510 kb on + strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.511 kb on - strand, within Pf1N1B4_379 at 449.522 kb on + strand, within Pf1N1B4_379 at 449.523 kb on - strand, within Pf1N1B4_379
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 446,557 + Pf1N1B4_375 0.66 +0.7 446,557 + +0.7 446,557 + Pf1N1B4_375 0.66 +0.7 446,557 + Pf1N1B4_375 0.66 +0.7 446,558 - Pf1N1B4_375 0.66 -0.9 446,558 - Pf1N1B4_375 0.66 +0.7 446,847 + -2.3 446,847 + Pf1N1B4_375 0.82 +1.7 446,847 + Pf1N1B4_375 0.82 +0.7 446,902 + Pf1N1B4_375 0.86 +0.7 446,902 + Pf1N1B4_375 0.86 -0.1 446,903 - Pf1N1B4_375 0.86 +0.7 446,990 + +0.7 447,042 + -1.2 447,043 - -1.1 447,135 - +0.6 447,436 + +0.0 447,436 + -2.5 447,437 - +0.7 447,437 - +0.7 447,753 - Pf1N1B4_376 0.29 -3.2 447,753 - Pf1N1B4_376 0.29 +0.7 448,216 + Pf1N1B4_376 0.68 -1.0 448,222 + Pf1N1B4_376 0.69 -0.4 448,298 - Pf1N1B4_376 0.75 -1.8 448,298 - Pf1N1B4_376 0.75 -3.5 448,298 - Pf1N1B4_376 0.75 -2.5 448,298 - Pf1N1B4_376 0.75 -2.6 448,438 + Pf1N1B4_376 0.87 -1.9 448,438 + Pf1N1B4_376 0.87 -0.9 448,439 - Pf1N1B4_376 0.87 -2.1 448,439 - Pf1N1B4_376 0.87 -0.8 448,439 - Pf1N1B4_376 0.87 -2.6 448,439 - Pf1N1B4_376 0.87 +0.7 448,747 - -0.7 448,762 - -2.5 448,804 + -2.7 448,804 + +0.7 449,082 - Pf1N1B4_378 0.24 -0.2 449,082 - Pf1N1B4_378 0.24 +0.7 449,082 - Pf1N1B4_378 0.24 +0.6 449,082 - Pf1N1B4_378 0.24 -1.9 449,098 + Pf1N1B4_378 0.32 +0.7 449,098 + Pf1N1B4_378 0.32 -0.7 449,098 + Pf1N1B4_378 0.32 -1.3 449,099 - Pf1N1B4_378 0.33 -1.8 449,099 - Pf1N1B4_378 0.33 -1.2 449,099 - Pf1N1B4_378 0.33 -0.4 449,099 - Pf1N1B4_378 0.33 -2.9 449,099 - Pf1N1B4_378 0.33 -0.8 449,216 - +0.2 449,312 - +0.5 449,312 - +1.7 449,358 + -0.6 449,359 - -0.9 449,510 + Pf1N1B4_379 0.40 -0.2 449,510 + Pf1N1B4_379 0.40 +0.1 449,510 + Pf1N1B4_379 0.40 -2.4 449,511 - Pf1N1B4_379 0.40 -0.7 449,511 - Pf1N1B4_379 0.40 +0.2 449,511 - Pf1N1B4_379 0.40 -0.9 449,511 - Pf1N1B4_379 0.40 -3.1 449,511 - Pf1N1B4_379 0.40 -1.1 449,511 - Pf1N1B4_379 0.40 -2.4 449,522 + Pf1N1B4_379 0.44 +0.1 449,523 - Pf1N1B4_379 0.44 +0.7
Or see this region's nucleotide sequence