Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3699

Experiment: N2E2wt with N1B4m denitrifying repB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3698 and Pf1N1B4_3699 are separated by 389 nucleotidesPf1N1B4_3699 and Pf1N1B4_3700 are separated by 30 nucleotides Pf1N1B4_3698 - Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), at 4,106,311 to 4,107,369 _3698 Pf1N1B4_3699 - FIG00954480: hypothetical protein, at 4,107,759 to 4,108,817 _3699 Pf1N1B4_3700 - Putative permease often clustered with de novo purine synthesis, at 4,108,848 to 4,109,921 _3700 Position (kb) 4107 4108 4109Strain fitness (log2 ratio) -2 -1 0 1at 4106.925 kb on - strandat 4107.604 kb on + strandat 4107.604 kb on + strandat 4107.604 kb on + strandat 4107.604 kb on + strandat 4107.604 kb on + strandat 4107.605 kb on - strandat 4107.605 kb on - strandat 4107.605 kb on - strandat 4107.606 kb on + strandat 4107.606 kb on + strandat 4107.607 kb on - strandat 4107.634 kb on + strandat 4108.397 kb on - strand, within Pf1N1B4_3699at 4108.397 kb on - strand, within Pf1N1B4_3699at 4108.826 kb on + strandat 4108.827 kb on - strandat 4108.827 kb on - strandat 4108.827 kb on - strandat 4108.828 kb on + strandat 4108.828 kb on + strandat 4108.828 kb on + strandat 4108.829 kb on - strandat 4108.830 kb on + strandat 4108.831 kb on - strandat 4108.831 kb on - strandat 4108.845 kb on + strandat 4108.845 kb on + strandat 4108.908 kb on + strandat 4108.959 kb on + strand, within Pf1N1B4_3700at 4108.960 kb on - strand, within Pf1N1B4_3700at 4108.960 kb on - strand, within Pf1N1B4_3700at 4109.110 kb on - strand, within Pf1N1B4_3700at 4109.110 kb on - strand, within Pf1N1B4_3700at 4109.110 kb on - strand, within Pf1N1B4_3700at 4109.505 kb on + strand, within Pf1N1B4_3700at 4109.506 kb on - strand, within Pf1N1B4_3700at 4109.506 kb on - strandat 4109.586 kb on + strand, within Pf1N1B4_3700at 4109.587 kb on - strand, within Pf1N1B4_3700at 4109.587 kb on - strand, within Pf1N1B4_3700at 4109.656 kb on - strand, within Pf1N1B4_3700

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
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4,106,925 - -1.0
4,107,604 + +1.4
4,107,604 + -0.3
4,107,604 + -0.4
4,107,604 + -1.9
4,107,604 + +0.9
4,107,605 - +0.8
4,107,605 - +0.7
4,107,605 - +0.7
4,107,606 + -1.6
4,107,606 + -0.3
4,107,607 - +0.7
4,107,634 + -0.0
4,108,397 - Pf1N1B4_3699 0.60 +0.7
4,108,397 - Pf1N1B4_3699 0.60 +0.7
4,108,826 + +0.7
4,108,827 - +0.7
4,108,827 - -0.9
4,108,827 - -1.3
4,108,828 + -0.8
4,108,828 + -0.5
4,108,828 + +0.7
4,108,829 - -0.9
4,108,830 + -0.8
4,108,831 - -1.2
4,108,831 - +0.7
4,108,845 + -0.7
4,108,845 + -2.3
4,108,908 + -0.3
4,108,959 + Pf1N1B4_3700 0.10 +0.7
4,108,960 - Pf1N1B4_3700 0.10 +0.7
4,108,960 - Pf1N1B4_3700 0.10 -0.3
4,109,110 - Pf1N1B4_3700 0.24 -1.4
4,109,110 - Pf1N1B4_3700 0.24 +0.4
4,109,110 - Pf1N1B4_3700 0.24 +0.7
4,109,505 + Pf1N1B4_3700 0.61 -2.1
4,109,506 - Pf1N1B4_3700 0.61 +0.7
4,109,506 - +0.7
4,109,586 + Pf1N1B4_3700 0.69 +0.7
4,109,587 - Pf1N1B4_3700 0.69 -1.1
4,109,587 - Pf1N1B4_3700 0.69 +0.7
4,109,656 - Pf1N1B4_3700 0.75 -0.3

Or see this region's nucleotide sequence