Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3604

Experiment: N2E2wt with N1B4m denitrifying repB

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPf1N1B4_3602 and Pf1N1B4_3603 are separated by 190 nucleotidesPf1N1B4_3603 and Pf1N1B4_3604 are separated by 15 nucleotidesPf1N1B4_3604 and Pf1N1B4_3605 overlap by 8 nucleotides Pf1N1B4_3602 - Flagellar regulatory protein FleQ, at 4,013,344 to 4,013,931 _3602 Pf1N1B4_3603 - Flagellar hook-basal body complex protein FliE, at 4,014,122 to 4,014,451 _3603 Pf1N1B4_3604 - Flagellar M-ring protein FliF, at 4,014,467 to 4,016,254 _3604 Pf1N1B4_3605 - Flagellar motor switch protein FliG, at 4,016,247 to 4,017,266 _3605 Position (kb) 4014 4015 4016 4017Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4013.870 kb on - strandat 4013.914 kb on + strandat 4013.914 kb on + strandat 4013.914 kb on + strandat 4013.915 kb on - strandat 4013.915 kb on - strandat 4013.915 kb on - strandat 4014.099 kb on + strandat 4014.099 kb on + strandat 4014.100 kb on - strandat 4014.100 kb on - strandat 4014.100 kb on - strandat 4014.100 kb on - strandat 4014.100 kb on - strandat 4014.422 kb on + strandat 4014.513 kb on + strandat 4014.672 kb on - strand, within Pf1N1B4_3604at 4015.024 kb on + strand, within Pf1N1B4_3604at 4015.024 kb on + strandat 4015.025 kb on - strand, within Pf1N1B4_3604at 4015.055 kb on + strand, within Pf1N1B4_3604at 4015.055 kb on + strand, within Pf1N1B4_3604at 4015.055 kb on + strand, within Pf1N1B4_3604at 4015.263 kb on + strand, within Pf1N1B4_3604at 4015.263 kb on + strand, within Pf1N1B4_3604at 4015.264 kb on - strand, within Pf1N1B4_3604at 4015.301 kb on + strand, within Pf1N1B4_3604at 4015.301 kb on + strand, within Pf1N1B4_3604at 4015.303 kb on + strand, within Pf1N1B4_3604at 4015.911 kb on - strand, within Pf1N1B4_3604at 4015.911 kb on - strand, within Pf1N1B4_3604at 4016.493 kb on + strand, within Pf1N1B4_3605at 4016.493 kb on + strand, within Pf1N1B4_3605at 4016.671 kb on - strand, within Pf1N1B4_3605at 4016.671 kb on - strandat 4016.910 kb on - strand, within Pf1N1B4_3605at 4017.209 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
remove
4,013,870 - +0.7
4,013,914 + +2.3
4,013,914 + -2.1
4,013,914 + +0.7
4,013,915 - +0.7
4,013,915 - +0.7
4,013,915 - -0.1
4,014,099 + +0.7
4,014,099 + -0.9
4,014,100 - +0.7
4,014,100 - -4.5
4,014,100 - +0.5
4,014,100 - +0.7
4,014,100 - +0.7
4,014,422 + +0.7
4,014,513 + -0.3
4,014,672 - Pf1N1B4_3604 0.11 -2.9
4,015,024 + Pf1N1B4_3604 0.31 -1.4
4,015,024 + +0.7
4,015,025 - Pf1N1B4_3604 0.31 -1.8
4,015,055 + Pf1N1B4_3604 0.33 -1.6
4,015,055 + Pf1N1B4_3604 0.33 -0.2
4,015,055 + Pf1N1B4_3604 0.33 -1.1
4,015,263 + Pf1N1B4_3604 0.45 -2.5
4,015,263 + Pf1N1B4_3604 0.45 -1.3
4,015,264 - Pf1N1B4_3604 0.45 +0.7
4,015,301 + Pf1N1B4_3604 0.47 -0.7
4,015,301 + Pf1N1B4_3604 0.47 -2.0
4,015,303 + Pf1N1B4_3604 0.47 -2.5
4,015,911 - Pf1N1B4_3604 0.81 -2.9
4,015,911 - Pf1N1B4_3604 0.81 +0.7
4,016,493 + Pf1N1B4_3605 0.24 -1.1
4,016,493 + Pf1N1B4_3605 0.24 +0.7
4,016,671 - Pf1N1B4_3605 0.42 -2.5
4,016,671 - +0.7
4,016,910 - Pf1N1B4_3605 0.65 +0.7
4,017,209 + -1.3

Or see this region's nucleotide sequence