Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3602 and Pf1N1B4_3603 are separated by 190 nucleotides Pf1N1B4_3603 and Pf1N1B4_3604 are separated by 15 nucleotides Pf1N1B4_3604 and Pf1N1B4_3605 overlap by 8 nucleotides
Pf1N1B4_3602 - Flagellar regulatory protein FleQ, at 4,013,344 to 4,013,931
_3602
Pf1N1B4_3603 - Flagellar hook-basal body complex protein FliE, at 4,014,122 to 4,014,451
_3603
Pf1N1B4_3604 - Flagellar M-ring protein FliF, at 4,014,467 to 4,016,254
_3604
Pf1N1B4_3605 - Flagellar motor switch protein FliG, at 4,016,247 to 4,017,266
_3605
Position (kb)
4014
4015
4016
4017 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4013.870 kb on - strand at 4013.914 kb on + strand at 4013.914 kb on + strand at 4013.914 kb on + strand at 4013.915 kb on - strand at 4013.915 kb on - strand at 4013.915 kb on - strand at 4014.099 kb on + strand at 4014.099 kb on + strand at 4014.100 kb on - strand at 4014.100 kb on - strand at 4014.100 kb on - strand at 4014.100 kb on - strand at 4014.100 kb on - strand at 4014.422 kb on + strand at 4014.513 kb on + strand at 4014.672 kb on - strand, within Pf1N1B4_3604 at 4015.024 kb on + strand, within Pf1N1B4_3604 at 4015.024 kb on + strand at 4015.025 kb on - strand, within Pf1N1B4_3604 at 4015.055 kb on + strand, within Pf1N1B4_3604 at 4015.055 kb on + strand, within Pf1N1B4_3604 at 4015.055 kb on + strand, within Pf1N1B4_3604 at 4015.263 kb on + strand, within Pf1N1B4_3604 at 4015.263 kb on + strand, within Pf1N1B4_3604 at 4015.264 kb on - strand, within Pf1N1B4_3604 at 4015.301 kb on + strand, within Pf1N1B4_3604 at 4015.301 kb on + strand, within Pf1N1B4_3604 at 4015.303 kb on + strand, within Pf1N1B4_3604 at 4015.911 kb on - strand, within Pf1N1B4_3604 at 4015.911 kb on - strand, within Pf1N1B4_3604 at 4016.493 kb on + strand, within Pf1N1B4_3605 at 4016.493 kb on + strand, within Pf1N1B4_3605 at 4016.671 kb on - strand, within Pf1N1B4_3605 at 4016.671 kb on - strand at 4016.910 kb on - strand, within Pf1N1B4_3605 at 4017.209 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 4,013,870 - +0.7 4,013,914 + +2.3 4,013,914 + -2.1 4,013,914 + +0.7 4,013,915 - +0.7 4,013,915 - +0.7 4,013,915 - -0.1 4,014,099 + +0.7 4,014,099 + -0.9 4,014,100 - +0.7 4,014,100 - -4.5 4,014,100 - +0.5 4,014,100 - +0.7 4,014,100 - +0.7 4,014,422 + +0.7 4,014,513 + -0.3 4,014,672 - Pf1N1B4_3604 0.11 -2.9 4,015,024 + Pf1N1B4_3604 0.31 -1.4 4,015,024 + +0.7 4,015,025 - Pf1N1B4_3604 0.31 -1.8 4,015,055 + Pf1N1B4_3604 0.33 -1.6 4,015,055 + Pf1N1B4_3604 0.33 -0.2 4,015,055 + Pf1N1B4_3604 0.33 -1.1 4,015,263 + Pf1N1B4_3604 0.45 -2.5 4,015,263 + Pf1N1B4_3604 0.45 -1.3 4,015,264 - Pf1N1B4_3604 0.45 +0.7 4,015,301 + Pf1N1B4_3604 0.47 -0.7 4,015,301 + Pf1N1B4_3604 0.47 -2.0 4,015,303 + Pf1N1B4_3604 0.47 -2.5 4,015,911 - Pf1N1B4_3604 0.81 -2.9 4,015,911 - Pf1N1B4_3604 0.81 +0.7 4,016,493 + Pf1N1B4_3605 0.24 -1.1 4,016,493 + Pf1N1B4_3605 0.24 +0.7 4,016,671 - Pf1N1B4_3605 0.42 -2.5 4,016,671 - +0.7 4,016,910 - Pf1N1B4_3605 0.65 +0.7 4,017,209 + -1.3
Or see this region's nucleotide sequence