Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3478 and Pf1N1B4_3479 overlap by 4 nucleotides Pf1N1B4_3479 and Pf1N1B4_3480 are separated by 132 nucleotides
Pf1N1B4_3478 - Transcriptional regulatory protein RstA, at 3,884,929 to 3,885,651
_3478
Pf1N1B4_3479 - Sensor histidine kinase, at 3,885,648 to 3,887,258
_3479
Pf1N1B4_3480 - 4'-phosphopantetheinyl transferase entD (EC 2.7.8.-); Holo-[acyl-carrier protein] synthase, alternative (EC 2.7.8.7), at 3,887,391 to 3,888,050
_3480
Position (kb)
3885
3886
3887
3888 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3884.680 kb on + strand at 3884.692 kb on + strand at 3884.693 kb on - strand at 3884.799 kb on + strand at 3884.830 kb on - strand at 3884.831 kb on + strand at 3884.832 kb on - strand at 3884.832 kb on - strand at 3884.832 kb on - strand at 3884.859 kb on - strand at 3884.875 kb on - strand at 3884.900 kb on + strand at 3884.998 kb on + strand at 3884.998 kb on + strand at 3884.999 kb on - strand at 3885.086 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.232 kb on + strand, within Pf1N1B4_3478 at 3885.233 kb on - strand, within Pf1N1B4_3478 at 3885.233 kb on - strand, within Pf1N1B4_3478 at 3885.233 kb on - strand, within Pf1N1B4_3478 at 3885.233 kb on - strand, within Pf1N1B4_3478 at 3885.457 kb on - strand, within Pf1N1B4_3478 at 3885.476 kb on + strand, within Pf1N1B4_3478 at 3885.476 kb on + strand, within Pf1N1B4_3478 at 3885.477 kb on - strand, within Pf1N1B4_3478 at 3885.477 kb on - strand, within Pf1N1B4_3478 at 3885.672 kb on + strand at 3885.672 kb on + strand at 3885.672 kb on + strand at 3885.673 kb on - strand at 3886.068 kb on + strand, within Pf1N1B4_3479 at 3886.068 kb on + strand, within Pf1N1B4_3479 at 3886.068 kb on + strand, within Pf1N1B4_3479 at 3886.069 kb on - strand, within Pf1N1B4_3479 at 3886.069 kb on - strand, within Pf1N1B4_3479 at 3886.069 kb on - strand, within Pf1N1B4_3479 at 3886.320 kb on + strand, within Pf1N1B4_3479 at 3886.320 kb on + strand, within Pf1N1B4_3479 at 3886.320 kb on + strand, within Pf1N1B4_3479 at 3886.746 kb on + strand, within Pf1N1B4_3479 at 3886.746 kb on + strand, within Pf1N1B4_3479 at 3886.746 kb on + strand at 3886.746 kb on + strand, within Pf1N1B4_3479 at 3886.747 kb on - strand, within Pf1N1B4_3479 at 3886.747 kb on - strand, within Pf1N1B4_3479 at 3886.747 kb on - strand, within Pf1N1B4_3479 at 3886.747 kb on - strand, within Pf1N1B4_3479 at 3886.923 kb on + strand, within Pf1N1B4_3479 at 3886.923 kb on + strand, within Pf1N1B4_3479 at 3886.923 kb on + strand, within Pf1N1B4_3479 at 3886.924 kb on - strand, within Pf1N1B4_3479 at 3886.951 kb on + strand, within Pf1N1B4_3479 at 3886.952 kb on - strand, within Pf1N1B4_3479 at 3886.952 kb on - strand, within Pf1N1B4_3479 at 3886.952 kb on - strand, within Pf1N1B4_3479 at 3886.955 kb on + strand, within Pf1N1B4_3479 at 3886.955 kb on + strand, within Pf1N1B4_3479 at 3886.956 kb on - strand, within Pf1N1B4_3479 at 3886.996 kb on - strand, within Pf1N1B4_3479 at 3887.023 kb on + strand, within Pf1N1B4_3479 at 3887.024 kb on - strand, within Pf1N1B4_3479 at 3887.383 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 3,884,680 + -1.3 3,884,692 + -0.9 3,884,693 - -2.1 3,884,799 + +0.7 3,884,830 - -1.0 3,884,831 + -0.2 3,884,832 - -1.6 3,884,832 - -0.3 3,884,832 - +0.7 3,884,859 - +0.7 3,884,875 - -0.2 3,884,900 + +0.7 3,884,998 + +1.7 3,884,998 + -2.1 3,884,999 - +0.3 3,885,086 + Pf1N1B4_3478 0.22 +0.4 3,885,151 + Pf1N1B4_3478 0.31 -1.4 3,885,151 + Pf1N1B4_3478 0.31 -3.0 3,885,151 + Pf1N1B4_3478 0.31 +0.7 3,885,151 + Pf1N1B4_3478 0.31 +0.7 3,885,151 + Pf1N1B4_3478 0.31 -1.6 3,885,152 - Pf1N1B4_3478 0.31 -0.4 3,885,152 - Pf1N1B4_3478 0.31 -1.7 3,885,152 - Pf1N1B4_3478 0.31 +0.7 3,885,152 - Pf1N1B4_3478 0.31 -1.4 3,885,152 - Pf1N1B4_3478 0.31 +0.7 3,885,152 - Pf1N1B4_3478 0.31 -1.7 3,885,152 - Pf1N1B4_3478 0.31 +0.7 3,885,152 - Pf1N1B4_3478 0.31 -0.3 3,885,152 - Pf1N1B4_3478 0.31 -0.4 3,885,232 + Pf1N1B4_3478 0.42 -1.9 3,885,233 - Pf1N1B4_3478 0.42 +0.7 3,885,233 - Pf1N1B4_3478 0.42 +0.0 3,885,233 - Pf1N1B4_3478 0.42 +0.7 3,885,233 - Pf1N1B4_3478 0.42 +0.7 3,885,457 - Pf1N1B4_3478 0.73 +0.7 3,885,476 + Pf1N1B4_3478 0.76 -3.9 3,885,476 + Pf1N1B4_3478 0.76 -2.1 3,885,477 - Pf1N1B4_3478 0.76 -3.2 3,885,477 - Pf1N1B4_3478 0.76 -0.4 3,885,672 + +0.8 3,885,672 + +0.7 3,885,672 + +0.7 3,885,673 - +1.0 3,886,068 + Pf1N1B4_3479 0.26 -0.3 3,886,068 + Pf1N1B4_3479 0.26 -2.6 3,886,068 + Pf1N1B4_3479 0.26 -0.1 3,886,069 - Pf1N1B4_3479 0.26 -1.9 3,886,069 - Pf1N1B4_3479 0.26 +1.1 3,886,069 - Pf1N1B4_3479 0.26 -1.6 3,886,320 + Pf1N1B4_3479 0.42 +0.7 3,886,320 + Pf1N1B4_3479 0.42 -0.9 3,886,320 + Pf1N1B4_3479 0.42 +0.7 3,886,746 + Pf1N1B4_3479 0.68 +0.7 3,886,746 + Pf1N1B4_3479 0.68 -1.4 3,886,746 + +0.6 3,886,746 + Pf1N1B4_3479 0.68 -0.9 3,886,747 - Pf1N1B4_3479 0.68 +0.7 3,886,747 - Pf1N1B4_3479 0.68 -2.6 3,886,747 - Pf1N1B4_3479 0.68 -1.4 3,886,747 - Pf1N1B4_3479 0.68 -1.3 3,886,923 + Pf1N1B4_3479 0.79 +0.7 3,886,923 + Pf1N1B4_3479 0.79 -1.1 3,886,923 + Pf1N1B4_3479 0.79 +0.7 3,886,924 - Pf1N1B4_3479 0.79 -2.5 3,886,951 + Pf1N1B4_3479 0.81 -1.1 3,886,952 - Pf1N1B4_3479 0.81 +1.0 3,886,952 - Pf1N1B4_3479 0.81 -2.6 3,886,952 - Pf1N1B4_3479 0.81 +0.1 3,886,955 + Pf1N1B4_3479 0.81 -0.9 3,886,955 + Pf1N1B4_3479 0.81 +0.7 3,886,956 - Pf1N1B4_3479 0.81 -0.4 3,886,996 - Pf1N1B4_3479 0.84 +0.7 3,887,023 + Pf1N1B4_3479 0.85 +0.7 3,887,024 - Pf1N1B4_3479 0.85 -0.5 3,887,383 - -1.9
Or see this region's nucleotide sequence