Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3335 and Pf1N1B4_5220 are separated by 83 nucleotides Pf1N1B4_5220 and Pf1N1B4_3336 are separated by 333 nucleotides Pf1N1B4_3336 and Pf1N1B4_3337 are separated by 163 nucleotides Pf1N1B4_3337 and Pf1N1B4_3338 are separated by 53 nucleotides Pf1N1B4_3338 and Pf1N1B4_3339 are separated by 3 nucleotides
Pf1N1B4_3335 - Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6), at 3,722,596 to 3,723,309
_3335
Pf1N1B4_5220 - tRNA-Pseudo-CGA, at 3,723,393 to 3,723,479
_5220
Pf1N1B4_3336 - UPF0339 protein NMA1193/NMA1859, at 3,723,813 to 3,724,004
_3336
Pf1N1B4_3337 - Transcriptional regulator, LysR family, at 3,724,168 to 3,725,097
_3337
Pf1N1B4_3338 - Probable 5-carboxymethyl-2-hydroxymuconate delta isomerase, at 3,725,151 to 3,725,516
_3338
Pf1N1B4_3339 - Transcriptional regulator, LysR family, at 3,725,520 to 3,726,455
_3339
Position (kb)
3724
3725
3726 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3723.327 kb on + strand at 3723.327 kb on + strand at 3723.377 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.487 kb on + strand at 3723.487 kb on + strand at 3723.487 kb on + strand at 3723.487 kb on + strand at 3723.499 kb on + strand at 3723.511 kb on + strand at 3723.606 kb on + strand at 3723.615 kb on + strand at 3723.636 kb on + strand at 3723.655 kb on + strand at 3723.655 kb on + strand at 3723.656 kb on - strand at 3723.656 kb on - strand at 3723.671 kb on + strand at 3723.671 kb on + strand at 3723.672 kb on - strand at 3723.672 kb on - strand at 3723.672 kb on - strand at 3723.672 kb on - strand at 3723.703 kb on - strand at 3723.703 kb on - strand at 3723.713 kb on + strand at 3723.756 kb on + strand at 3723.756 kb on + strand at 3723.756 kb on + strand at 3723.757 kb on - strand at 3723.757 kb on - strand at 3723.757 kb on - strand at 3723.816 kb on + strand at 3723.816 kb on + strand at 3723.817 kb on - strand at 3723.817 kb on - strand at 3723.924 kb on + strand, within Pf1N1B4_3336 at 3723.924 kb on + strand, within Pf1N1B4_3336 at 3723.924 kb on + strand, within Pf1N1B4_3336 at 3723.924 kb on + strand, within Pf1N1B4_3336 at 3723.924 kb on + strand at 3723.924 kb on + strand at 3723.924 kb on + strand, within Pf1N1B4_3336 at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3723.925 kb on - strand at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3723.925 kb on - strand, within Pf1N1B4_3336 at 3724.000 kb on + strand at 3724.000 kb on + strand at 3724.000 kb on + strand at 3724.000 kb on + strand at 3724.000 kb on + strand at 3724.001 kb on - strand at 3724.001 kb on - strand at 3724.001 kb on - strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.002 kb on + strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.003 kb on - strand at 3724.063 kb on - strand at 3724.064 kb on + strand at 3724.064 kb on + strand at 3724.064 kb on + strand at 3724.064 kb on + strand at 3724.064 kb on + strand at 3724.064 kb on + strand at 3724.064 kb on + strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.065 kb on - strand at 3724.109 kb on + strand at 3724.109 kb on + strand at 3724.110 kb on - strand at 3724.110 kb on - strand at 3724.110 kb on - strand at 3724.110 kb on - strand at 3724.110 kb on - strand at 3724.580 kb on - strand, within Pf1N1B4_3337 at 3724.626 kb on + strand, within Pf1N1B4_3337 at 3724.626 kb on + strand, within Pf1N1B4_3337 at 3724.626 kb on + strand, within Pf1N1B4_3337 at 3724.626 kb on + strand, within Pf1N1B4_3337 at 3724.692 kb on + strand, within Pf1N1B4_3337 at 3724.692 kb on + strand, within Pf1N1B4_3337 at 3724.693 kb on - strand, within Pf1N1B4_3337 at 3724.738 kb on + strand, within Pf1N1B4_3337 at 3724.738 kb on + strand, within Pf1N1B4_3337 at 3724.738 kb on + strand, within Pf1N1B4_3337 at 3724.738 kb on + strand, within Pf1N1B4_3337 at 3724.738 kb on + strand, within Pf1N1B4_3337 at 3724.821 kb on + strand, within Pf1N1B4_3337 at 3724.821 kb on + strand, within Pf1N1B4_3337 at 3724.821 kb on + strand at 3724.833 kb on - strand, within Pf1N1B4_3337 at 3724.833 kb on - strand, within Pf1N1B4_3337 at 3724.909 kb on + strand, within Pf1N1B4_3337 at 3725.111 kb on + strand at 3725.111 kb on + strand at 3725.111 kb on + strand at 3725.111 kb on + strand at 3725.111 kb on + strand at 3725.111 kb on + strand at 3725.111 kb on + strand at 3725.112 kb on - strand at 3725.112 kb on - strand at 3725.112 kb on - strand at 3725.112 kb on - strand at 3725.112 kb on - strand at 3725.112 kb on - strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.113 kb on + strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.114 kb on - strand at 3725.117 kb on + strand at 3725.117 kb on + strand at 3725.118 kb on - strand at 3725.172 kb on + strand at 3725.172 kb on + strand at 3725.172 kb on + strand at 3725.172 kb on + strand at 3725.173 kb on - strand at 3725.490 kb on + strand at 3725.490 kb on + strand at 3725.491 kb on - strand at 3725.491 kb on - strand at 3725.491 kb on - strand at 3725.747 kb on + strand, within Pf1N1B4_3339 at 3725.749 kb on + strand, within Pf1N1B4_3339 at 3725.750 kb on - strand, within Pf1N1B4_3339 at 3725.750 kb on - strand, within Pf1N1B4_3339 at 3725.769 kb on + strand, within Pf1N1B4_3339 at 3725.769 kb on + strand, within Pf1N1B4_3339 at 3725.769 kb on + strand, within Pf1N1B4_3339 at 3725.770 kb on - strand, within Pf1N1B4_3339 at 3725.813 kb on + strand, within Pf1N1B4_3339 at 3725.813 kb on + strand, within Pf1N1B4_3339 at 3725.813 kb on + strand, within Pf1N1B4_3339 at 3725.814 kb on - strand, within Pf1N1B4_3339
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 3,723,327 + -1.0 3,723,327 + +0.4 3,723,377 + -0.4 3,723,485 + -2.0 3,723,485 + -0.3 3,723,485 + -0.7 3,723,485 + -0.8 3,723,485 + +0.8 3,723,485 + -0.4 3,723,485 + -2.7 3,723,487 + +0.8 3,723,487 + +0.8 3,723,487 + -1.1 3,723,487 + -2.7 3,723,499 + -1.9 3,723,511 + -1.1 3,723,606 + -0.5 3,723,615 + +0.5 3,723,636 + +0.0 3,723,655 + -0.8 3,723,655 + -0.7 3,723,656 - -1.8 3,723,656 - +0.8 3,723,671 + -2.0 3,723,671 + -0.3 3,723,672 - -1.9 3,723,672 - -0.8 3,723,672 - +0.8 3,723,672 - -1.2 3,723,703 - -0.7 3,723,703 - +0.1 3,723,713 + +0.8 3,723,756 + +0.8 3,723,756 + +0.4 3,723,756 + +0.2 3,723,757 - -0.5 3,723,757 - -2.9 3,723,757 - -3.4 3,723,816 + -0.5 3,723,816 + -0.1 3,723,817 - -0.2 3,723,817 - -1.5 3,723,924 + Pf1N1B4_3336 0.58 -3.0 3,723,924 + Pf1N1B4_3336 0.58 -2.6 3,723,924 + Pf1N1B4_3336 0.58 +0.8 3,723,924 + Pf1N1B4_3336 0.58 -0.5 3,723,924 + +1.2 3,723,924 + +1.4 3,723,924 + Pf1N1B4_3336 0.58 -1.1 3,723,925 - Pf1N1B4_3336 0.58 -0.9 3,723,925 - Pf1N1B4_3336 0.58 -2.4 3,723,925 - Pf1N1B4_3336 0.58 -0.4 3,723,925 - Pf1N1B4_3336 0.58 +1.5 3,723,925 - +0.8 3,723,925 - Pf1N1B4_3336 0.58 +0.8 3,723,925 - Pf1N1B4_3336 0.58 -4.1 3,723,925 - Pf1N1B4_3336 0.58 -1.6 3,723,925 - Pf1N1B4_3336 0.58 -1.6 3,724,000 + +0.8 3,724,000 + -1.8 3,724,000 + +0.8 3,724,000 + -0.2 3,724,000 + -0.9 3,724,001 - -0.5 3,724,001 - +0.2 3,724,001 - -2.9 3,724,002 + -0.5 3,724,002 + -1.2 3,724,002 + -0.0 3,724,002 + -1.0 3,724,002 + -0.7 3,724,002 + -2.2 3,724,002 + -0.6 3,724,002 + -0.2 3,724,002 + -0.5 3,724,002 + -0.8 3,724,002 + -2.2 3,724,002 + +0.8 3,724,002 + +0.8 3,724,002 + +0.7 3,724,002 + +0.8 3,724,002 + -0.0 3,724,003 - -0.6 3,724,003 - +0.8 3,724,003 - +0.8 3,724,003 - -3.1 3,724,003 - +0.8 3,724,003 - +0.8 3,724,003 - +0.8 3,724,003 - -0.3 3,724,003 - -3.3 3,724,003 - +0.2 3,724,063 - -2.4 3,724,064 + +0.8 3,724,064 + -0.3 3,724,064 + -0.7 3,724,064 + -1.3 3,724,064 + +0.2 3,724,064 + +0.8 3,724,064 + -1.4 3,724,065 - +0.9 3,724,065 - +0.8 3,724,065 - -1.6 3,724,065 - +0.8 3,724,065 - +0.8 3,724,065 - +0.6 3,724,065 - -2.2 3,724,065 - -0.9 3,724,065 - -0.9 3,724,109 + +0.6 3,724,109 + -2.0 3,724,110 - +0.5 3,724,110 - -0.4 3,724,110 - +1.1 3,724,110 - -0.1 3,724,110 - +0.8 3,724,580 - Pf1N1B4_3337 0.44 -0.6 3,724,626 + Pf1N1B4_3337 0.49 -0.5 3,724,626 + Pf1N1B4_3337 0.49 +0.5 3,724,626 + Pf1N1B4_3337 0.49 +2.4 3,724,626 + Pf1N1B4_3337 0.49 -1.0 3,724,692 + Pf1N1B4_3337 0.56 -3.1 3,724,692 + Pf1N1B4_3337 0.56 +0.8 3,724,693 - Pf1N1B4_3337 0.56 -1.8 3,724,738 + Pf1N1B4_3337 0.61 +0.8 3,724,738 + Pf1N1B4_3337 0.61 -0.4 3,724,738 + Pf1N1B4_3337 0.61 +1.4 3,724,738 + Pf1N1B4_3337 0.61 +0.4 3,724,738 + Pf1N1B4_3337 0.61 +0.0 3,724,821 + Pf1N1B4_3337 0.70 +0.7 3,724,821 + Pf1N1B4_3337 0.70 -0.6 3,724,821 + +1.3 3,724,833 - Pf1N1B4_3337 0.72 +0.8 3,724,833 - Pf1N1B4_3337 0.72 +0.2 3,724,909 + Pf1N1B4_3337 0.80 +0.5 3,725,111 + -2.8 3,725,111 + -0.6 3,725,111 + +0.8 3,725,111 + -1.0 3,725,111 + -3.3 3,725,111 + -1.3 3,725,111 + -1.6 3,725,112 - +0.8 3,725,112 - -1.4 3,725,112 - +0.1 3,725,112 - +0.4 3,725,112 - -1.2 3,725,112 - +0.8 3,725,113 + -1.1 3,725,113 + -1.2 3,725,113 + -0.6 3,725,113 + +0.8 3,725,113 + +0.2 3,725,113 + -1.2 3,725,113 + -2.6 3,725,113 + -0.2 3,725,113 + -1.5 3,725,113 + -0.6 3,725,113 + +0.8 3,725,113 + -2.4 3,725,113 + +0.8 3,725,113 + +0.8 3,725,113 + +0.2 3,725,113 + +0.0 3,725,113 + +0.8 3,725,113 + -1.2 3,725,113 + -1.8 3,725,113 + -1.8 3,725,113 + +1.6 3,725,113 + +0.8 3,725,113 + +0.8 3,725,113 + -1.0 3,725,113 + +0.8 3,725,113 + -1.6 3,725,113 + +0.8 3,725,113 + -1.0 3,725,113 + -0.2 3,725,113 + -1.5 3,725,114 - -1.2 3,725,114 - +0.4 3,725,114 - -1.6 3,725,114 - +0.8 3,725,114 - +0.8 3,725,114 - +0.2 3,725,114 - -0.7 3,725,114 - +0.8 3,725,114 - -2.3 3,725,114 - -0.2 3,725,114 - +0.8 3,725,114 - +0.8 3,725,114 - +0.8 3,725,114 - -0.8 3,725,114 - +0.3 3,725,117 + -0.5 3,725,117 + -2.8 3,725,118 - -3.3 3,725,172 + -0.8 3,725,172 + +0.8 3,725,172 + -1.6 3,725,172 + +0.8 3,725,173 - -0.5 3,725,490 + +0.0 3,725,490 + +0.8 3,725,491 - -0.1 3,725,491 - +0.8 3,725,491 - +0.5 3,725,747 + Pf1N1B4_3339 0.24 -0.2 3,725,749 + Pf1N1B4_3339 0.24 -1.4 3,725,750 - Pf1N1B4_3339 0.25 -0.3 3,725,750 - Pf1N1B4_3339 0.25 -1.5 3,725,769 + Pf1N1B4_3339 0.27 +1.2 3,725,769 + Pf1N1B4_3339 0.27 +0.8 3,725,769 + Pf1N1B4_3339 0.27 -1.8 3,725,770 - Pf1N1B4_3339 0.27 -2.4 3,725,813 + Pf1N1B4_3339 0.31 +0.8 3,725,813 + Pf1N1B4_3339 0.31 +0.8 3,725,813 + Pf1N1B4_3339 0.31 +0.1 3,725,814 - Pf1N1B4_3339 0.31 -3.5
Or see this region's nucleotide sequence