Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3208

Experiment: N2E2wt with N1B4m denitrifying repB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3207 and Pf1N1B4_3208 are separated by 145 nucleotidesPf1N1B4_3208 and Pf1N1B4_3209 overlap by 4 nucleotidesPf1N1B4_3209 and Pf1N1B4_3210 overlap by 4 nucleotides Pf1N1B4_3207 - Transcriptional regulator, LysR family, at 3,590,870 to 3,591,778 _3207 Pf1N1B4_3208 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 3,591,924 to 3,592,427 _3208 Pf1N1B4_3209 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,592,424 to 3,593,182 _3209 Pf1N1B4_3210 - Uncharacterized protein conserved in bacteria, NMA0228-like, at 3,593,179 to 3,594,033 _3210 Position (kb) 3591 3592 3593Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3591.006 kb on + strand, within Pf1N1B4_3207at 3591.063 kb on + strand, within Pf1N1B4_3207at 3591.221 kb on + strand, within Pf1N1B4_3207at 3591.256 kb on + strand, within Pf1N1B4_3207at 3591.256 kb on + strand, within Pf1N1B4_3207at 3591.257 kb on - strand, within Pf1N1B4_3207at 3591.257 kb on - strand, within Pf1N1B4_3207at 3591.257 kb on - strand, within Pf1N1B4_3207at 3591.257 kb on - strand, within Pf1N1B4_3207at 3591.257 kb on - strand, within Pf1N1B4_3207at 3591.260 kb on + strandat 3591.260 kb on + strand, within Pf1N1B4_3207at 3591.260 kb on + strand, within Pf1N1B4_3207at 3591.260 kb on + strand, within Pf1N1B4_3207at 3591.380 kb on + strand, within Pf1N1B4_3207at 3591.380 kb on + strandat 3591.380 kb on + strand, within Pf1N1B4_3207at 3591.381 kb on - strand, within Pf1N1B4_3207at 3591.381 kb on - strand, within Pf1N1B4_3207at 3591.381 kb on - strand, within Pf1N1B4_3207at 3591.453 kb on - strand, within Pf1N1B4_3207at 3591.476 kb on + strand, within Pf1N1B4_3207at 3591.476 kb on + strandat 3591.477 kb on - strand, within Pf1N1B4_3207at 3591.495 kb on + strand, within Pf1N1B4_3207at 3591.495 kb on + strand, within Pf1N1B4_3207at 3591.496 kb on - strand, within Pf1N1B4_3207at 3591.536 kb on + strandat 3591.536 kb on + strand, within Pf1N1B4_3207at 3591.536 kb on + strand, within Pf1N1B4_3207at 3591.536 kb on + strand, within Pf1N1B4_3207at 3591.537 kb on - strand, within Pf1N1B4_3207at 3591.537 kb on - strand, within Pf1N1B4_3207at 3591.593 kb on + strand, within Pf1N1B4_3207at 3591.593 kb on + strand, within Pf1N1B4_3207at 3591.594 kb on - strandat 3591.594 kb on - strand, within Pf1N1B4_3207at 3591.671 kb on + strand, within Pf1N1B4_3207at 3591.671 kb on + strand, within Pf1N1B4_3207at 3591.671 kb on + strand, within Pf1N1B4_3207at 3591.671 kb on + strand, within Pf1N1B4_3207at 3591.672 kb on - strand, within Pf1N1B4_3207at 3591.776 kb on + strandat 3591.776 kb on + strandat 3591.777 kb on - strandat 3591.853 kb on + strandat 3591.853 kb on + strandat 3591.854 kb on - strandat 3591.854 kb on - strandat 3592.009 kb on + strand, within Pf1N1B4_3208at 3592.009 kb on + strand, within Pf1N1B4_3208at 3592.009 kb on + strand, within Pf1N1B4_3208at 3592.009 kb on + strand, within Pf1N1B4_3208at 3592.010 kb on - strand, within Pf1N1B4_3208at 3592.010 kb on - strand, within Pf1N1B4_3208at 3592.086 kb on - strand, within Pf1N1B4_3208at 3592.207 kb on + strand, within Pf1N1B4_3208at 3592.207 kb on + strand, within Pf1N1B4_3208at 3592.445 kb on + strandat 3592.445 kb on + strandat 3592.445 kb on + strandat 3592.445 kb on + strandat 3592.445 kb on + strandat 3592.445 kb on + strandat 3592.446 kb on - strandat 3592.446 kb on - strandat 3592.869 kb on + strand, within Pf1N1B4_3209at 3592.869 kb on + strand, within Pf1N1B4_3209at 3592.869 kb on + strand, within Pf1N1B4_3209at 3592.870 kb on - strand, within Pf1N1B4_3209at 3592.870 kb on - strand, within Pf1N1B4_3209at 3592.870 kb on - strand, within Pf1N1B4_3209at 3592.870 kb on - strand, within Pf1N1B4_3209at 3592.968 kb on + strand, within Pf1N1B4_3209at 3592.968 kb on + strand, within Pf1N1B4_3209at 3592.968 kb on + strand, within Pf1N1B4_3209at 3592.968 kb on + strand, within Pf1N1B4_3209at 3592.968 kb on + strand, within Pf1N1B4_3209at 3592.968 kb on + strand, within Pf1N1B4_3209at 3592.968 kb on + strand, within Pf1N1B4_3209at 3592.968 kb on + strand, within Pf1N1B4_3209at 3592.968 kb on + strand, within Pf1N1B4_3209at 3592.968 kb on + strand, within Pf1N1B4_3209at 3592.968 kb on + strand, within Pf1N1B4_3209at 3592.968 kb on + strand, within Pf1N1B4_3209at 3592.968 kb on + strand, within Pf1N1B4_3209at 3592.968 kb on + strand, within Pf1N1B4_3209at 3592.969 kb on - strand, within Pf1N1B4_3209at 3592.969 kb on - strandat 3592.969 kb on - strand, within Pf1N1B4_3209at 3593.062 kb on + strand, within Pf1N1B4_3209at 3593.063 kb on - strand, within Pf1N1B4_3209at 3593.063 kb on - strand, within Pf1N1B4_3209at 3593.073 kb on + strand, within Pf1N1B4_3209at 3593.073 kb on + strand, within Pf1N1B4_3209at 3593.074 kb on - strandat 3593.255 kb on - strandat 3593.315 kb on + strand, within Pf1N1B4_3210at 3593.315 kb on + strand, within Pf1N1B4_3210at 3593.388 kb on + strand, within Pf1N1B4_3210at 3593.389 kb on - strand, within Pf1N1B4_3210at 3593.389 kb on - strand, within Pf1N1B4_3210

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
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3,591,006 + Pf1N1B4_3207 0.15 +0.8
3,591,063 + Pf1N1B4_3207 0.21 -2.2
3,591,221 + Pf1N1B4_3207 0.39 -2.4
3,591,256 + Pf1N1B4_3207 0.42 -0.4
3,591,256 + Pf1N1B4_3207 0.42 -1.4
3,591,257 - Pf1N1B4_3207 0.43 -1.1
3,591,257 - Pf1N1B4_3207 0.43 +0.0
3,591,257 - Pf1N1B4_3207 0.43 -0.5
3,591,257 - Pf1N1B4_3207 0.43 -0.7
3,591,257 - Pf1N1B4_3207 0.43 -0.0
3,591,260 + -2.2
3,591,260 + Pf1N1B4_3207 0.43 +0.8
3,591,260 + Pf1N1B4_3207 0.43 -0.3
3,591,260 + Pf1N1B4_3207 0.43 +0.8
3,591,380 + Pf1N1B4_3207 0.56 -1.5
3,591,380 + -2.4
3,591,380 + Pf1N1B4_3207 0.56 -1.5
3,591,381 - Pf1N1B4_3207 0.56 -0.8
3,591,381 - Pf1N1B4_3207 0.56 -1.2
3,591,381 - Pf1N1B4_3207 0.56 -0.8
3,591,453 - Pf1N1B4_3207 0.64 +0.8
3,591,476 + Pf1N1B4_3207 0.67 +0.8
3,591,476 + +0.8
3,591,477 - Pf1N1B4_3207 0.67 +0.8
3,591,495 + Pf1N1B4_3207 0.69 +0.8
3,591,495 + Pf1N1B4_3207 0.69 -3.5
3,591,496 - Pf1N1B4_3207 0.69 -3.2
3,591,536 + +0.8
3,591,536 + Pf1N1B4_3207 0.73 -0.5
3,591,536 + Pf1N1B4_3207 0.73 +0.8
3,591,536 + Pf1N1B4_3207 0.73 +0.8
3,591,537 - Pf1N1B4_3207 0.73 -1.2
3,591,537 - Pf1N1B4_3207 0.73 +0.1
3,591,593 + Pf1N1B4_3207 0.80 -1.5
3,591,593 + Pf1N1B4_3207 0.80 +0.8
3,591,594 - -1.4
3,591,594 - Pf1N1B4_3207 0.80 -0.8
3,591,671 + Pf1N1B4_3207 0.88 +0.8
3,591,671 + Pf1N1B4_3207 0.88 -0.8
3,591,671 + Pf1N1B4_3207 0.88 +0.4
3,591,671 + Pf1N1B4_3207 0.88 +1.4
3,591,672 - Pf1N1B4_3207 0.88 -0.2
3,591,776 + +0.8
3,591,776 + -0.1
3,591,777 - -0.3
3,591,853 + +0.8
3,591,853 + -0.5
3,591,854 - -3.3
3,591,854 - -0.2
3,592,009 + Pf1N1B4_3208 0.17 -0.2
3,592,009 + Pf1N1B4_3208 0.17 -0.1
3,592,009 + Pf1N1B4_3208 0.17 -0.3
3,592,009 + Pf1N1B4_3208 0.17 +0.8
3,592,010 - Pf1N1B4_3208 0.17 -2.5
3,592,010 - Pf1N1B4_3208 0.17 +0.8
3,592,086 - Pf1N1B4_3208 0.32 -0.7
3,592,207 + Pf1N1B4_3208 0.56 +0.1
3,592,207 + Pf1N1B4_3208 0.56 +1.0
3,592,445 + -1.3
3,592,445 + -1.2
3,592,445 + -2.4
3,592,445 + +0.8
3,592,445 + -2.0
3,592,445 + +0.9
3,592,446 - +0.8
3,592,446 - +0.8
3,592,869 + Pf1N1B4_3209 0.59 +0.2
3,592,869 + Pf1N1B4_3209 0.59 -3.4
3,592,869 + Pf1N1B4_3209 0.59 +0.8
3,592,870 - Pf1N1B4_3209 0.59 -3.1
3,592,870 - Pf1N1B4_3209 0.59 +0.8
3,592,870 - Pf1N1B4_3209 0.59 +0.8
3,592,870 - Pf1N1B4_3209 0.59 +0.2
3,592,968 + Pf1N1B4_3209 0.72 -2.0
3,592,968 + Pf1N1B4_3209 0.72 -1.2
3,592,968 + Pf1N1B4_3209 0.72 +0.8
3,592,968 + Pf1N1B4_3209 0.72 +0.8
3,592,968 + Pf1N1B4_3209 0.72 +0.8
3,592,968 + Pf1N1B4_3209 0.72 -0.8
3,592,968 + Pf1N1B4_3209 0.72 -0.2
3,592,968 + Pf1N1B4_3209 0.72 +0.9
3,592,968 + Pf1N1B4_3209 0.72 +1.2
3,592,968 + Pf1N1B4_3209 0.72 -2.7
3,592,968 + Pf1N1B4_3209 0.72 -1.7
3,592,968 + Pf1N1B4_3209 0.72 -2.2
3,592,968 + Pf1N1B4_3209 0.72 -1.5
3,592,968 + Pf1N1B4_3209 0.72 +0.8
3,592,969 - Pf1N1B4_3209 0.72 -0.3
3,592,969 - +1.8
3,592,969 - Pf1N1B4_3209 0.72 -1.1
3,593,062 + Pf1N1B4_3209 0.84 -1.4
3,593,063 - Pf1N1B4_3209 0.84 -1.5
3,593,063 - Pf1N1B4_3209 0.84 -2.4
3,593,073 + Pf1N1B4_3209 0.86 +0.8
3,593,073 + Pf1N1B4_3209 0.86 -0.6
3,593,074 - +0.8
3,593,255 - +0.8
3,593,315 + Pf1N1B4_3210 0.16 +0.5
3,593,315 + Pf1N1B4_3210 0.16 -0.8
3,593,388 + Pf1N1B4_3210 0.24 +0.8
3,593,389 - Pf1N1B4_3210 0.25 +0.8
3,593,389 - Pf1N1B4_3210 0.25 -0.9

Or see this region's nucleotide sequence