Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2927 and Pf1N1B4_2928 overlap by 1 nucleotides Pf1N1B4_2928 and Pf1N1B4_2929 overlap by 1 nucleotides Pf1N1B4_2929 and Pf1N1B4_2930 are separated by 157 nucleotides
Pf1N1B4_2927 - Deoxyribodipyrimidine photolyase (EC 4.1.99.3), at 3,282,972 to 3,284,453
_2927
Pf1N1B4_2928 - Transcriptional regulator, MerR family, associated with photolyase, at 3,284,453 to 3,285,394
_2928
Pf1N1B4_2929 - COG1683: Uncharacterized conserved protein / FIG143828: Hypothetical protein YbgA, at 3,285,394 to 3,286,338
_2929
Pf1N1B4_2930 - COG3380: Amine oxidase, flavin-containing, at 3,286,496 to 3,287,482
_2930
Position (kb)
3285
3286
3287 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3284.427 kb on - strand at 3284.427 kb on - strand at 3284.446 kb on + strand at 3284.447 kb on - strand at 3284.447 kb on - strand at 3284.447 kb on - strand at 3284.447 kb on - strand at 3284.454 kb on + strand at 3284.454 kb on + strand at 3284.454 kb on + strand at 3284.455 kb on - strand at 3284.649 kb on + strand, within Pf1N1B4_2928 at 3284.649 kb on + strand, within Pf1N1B4_2928 at 3284.649 kb on + strand, within Pf1N1B4_2928 at 3284.650 kb on - strand, within Pf1N1B4_2928 at 3284.650 kb on - strand, within Pf1N1B4_2928 at 3284.650 kb on - strand, within Pf1N1B4_2928 at 3284.650 kb on - strand, within Pf1N1B4_2928 at 3284.650 kb on - strand, within Pf1N1B4_2928 at 3284.776 kb on - strand, within Pf1N1B4_2928 at 3284.776 kb on - strand, within Pf1N1B4_2928 at 3284.776 kb on - strand, within Pf1N1B4_2928 at 3284.776 kb on - strand, within Pf1N1B4_2928 at 3284.800 kb on - strand, within Pf1N1B4_2928 at 3284.800 kb on - strand, within Pf1N1B4_2928 at 3284.847 kb on + strand, within Pf1N1B4_2928 at 3284.847 kb on + strand, within Pf1N1B4_2928 at 3284.848 kb on - strand, within Pf1N1B4_2928 at 3284.883 kb on + strand, within Pf1N1B4_2928 at 3284.883 kb on + strand, within Pf1N1B4_2928 at 3284.960 kb on + strand, within Pf1N1B4_2928 at 3284.961 kb on - strand, within Pf1N1B4_2928 at 3284.961 kb on - strand, within Pf1N1B4_2928 at 3284.961 kb on - strand, within Pf1N1B4_2928 at 3284.961 kb on - strand, within Pf1N1B4_2928 at 3284.961 kb on - strand, within Pf1N1B4_2928 at 3284.961 kb on - strand, within Pf1N1B4_2928 at 3284.961 kb on - strand, within Pf1N1B4_2928 at 3285.008 kb on + strand, within Pf1N1B4_2928 at 3285.009 kb on - strand, within Pf1N1B4_2928 at 3285.009 kb on - strand, within Pf1N1B4_2928 at 3285.167 kb on - strand, within Pf1N1B4_2928 at 3285.167 kb on - strand, within Pf1N1B4_2928 at 3285.226 kb on + strand, within Pf1N1B4_2928 at 3285.227 kb on - strand, within Pf1N1B4_2928 at 3285.395 kb on + strand at 3285.441 kb on - strand at 3285.441 kb on - strand at 3285.459 kb on - strand at 3285.483 kb on - strand at 3285.483 kb on - strand at 3285.740 kb on + strand, within Pf1N1B4_2929 at 3285.741 kb on - strand, within Pf1N1B4_2929 at 3285.741 kb on - strand, within Pf1N1B4_2929 at 3285.741 kb on - strand, within Pf1N1B4_2929 at 3285.771 kb on - strand, within Pf1N1B4_2929 at 3285.771 kb on - strand, within Pf1N1B4_2929 at 3285.771 kb on - strand, within Pf1N1B4_2929 at 3285.771 kb on - strand, within Pf1N1B4_2929 at 3285.777 kb on - strand, within Pf1N1B4_2929 at 3285.954 kb on + strand, within Pf1N1B4_2929 at 3285.955 kb on - strand, within Pf1N1B4_2929 at 3286.039 kb on - strand, within Pf1N1B4_2929 at 3286.044 kb on + strand at 3286.045 kb on - strand, within Pf1N1B4_2929 at 3286.045 kb on - strand, within Pf1N1B4_2929 at 3286.045 kb on - strand, within Pf1N1B4_2929 at 3286.045 kb on - strand, within Pf1N1B4_2929 at 3286.047 kb on - strand at 3286.236 kb on - strand, within Pf1N1B4_2929 at 3286.287 kb on - strand at 3286.306 kb on + strand at 3286.344 kb on + strand at 3286.379 kb on - strand at 3286.385 kb on + strand at 3286.386 kb on - strand at 3286.388 kb on + strand at 3286.389 kb on - strand at 3286.389 kb on - strand at 3286.397 kb on - strand at 3286.414 kb on + strand at 3286.414 kb on + strand at 3286.415 kb on - strand at 3286.415 kb on - strand at 3286.428 kb on + strand at 3286.429 kb on - strand at 3286.590 kb on + strand at 3286.590 kb on + strand at 3286.590 kb on + strand at 3286.590 kb on + strand at 3286.590 kb on + strand at 3286.590 kb on + strand at 3286.590 kb on + strand at 3286.590 kb on + strand at 3286.590 kb on + strand at 3286.591 kb on - strand at 3286.591 kb on - strand at 3286.591 kb on - strand at 3286.591 kb on - strand at 3286.591 kb on - strand at 3286.591 kb on - strand at 3286.591 kb on - strand at 3286.591 kb on - strand at 3286.591 kb on - strand at 3286.591 kb on - strand at 3286.943 kb on + strand, within Pf1N1B4_2930 at 3286.944 kb on - strand, within Pf1N1B4_2930 at 3287.224 kb on + strand, within Pf1N1B4_2930 at 3287.224 kb on + strand, within Pf1N1B4_2930 at 3287.224 kb on + strand, within Pf1N1B4_2930 at 3287.224 kb on + strand, within Pf1N1B4_2930 at 3287.225 kb on - strand, within Pf1N1B4_2930 at 3287.225 kb on - strand, within Pf1N1B4_2930 at 3287.225 kb on - strand, within Pf1N1B4_2930 at 3287.226 kb on + strand, within Pf1N1B4_2930 at 3287.226 kb on + strand, within Pf1N1B4_2930 at 3287.226 kb on + strand at 3287.226 kb on + strand at 3287.226 kb on + strand, within Pf1N1B4_2930 at 3287.227 kb on - strand, within Pf1N1B4_2930 at 3287.227 kb on - strand, within Pf1N1B4_2930 at 3287.227 kb on - strand, within Pf1N1B4_2930 at 3287.227 kb on - strand, within Pf1N1B4_2930 at 3287.227 kb on - strand, within Pf1N1B4_2930 at 3287.313 kb on + strand, within Pf1N1B4_2930
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 3,284,427 - -2.0 3,284,427 - +0.8 3,284,446 + -0.2 3,284,447 - -1.8 3,284,447 - +0.0 3,284,447 - -0.1 3,284,447 - -0.8 3,284,454 + -1.6 3,284,454 + -1.6 3,284,454 + -2.4 3,284,455 - +0.8 3,284,649 + Pf1N1B4_2928 0.21 -1.8 3,284,649 + Pf1N1B4_2928 0.21 -2.7 3,284,649 + Pf1N1B4_2928 0.21 -2.1 3,284,650 - Pf1N1B4_2928 0.21 -3.1 3,284,650 - Pf1N1B4_2928 0.21 +0.8 3,284,650 - Pf1N1B4_2928 0.21 -0.2 3,284,650 - Pf1N1B4_2928 0.21 -1.6 3,284,650 - Pf1N1B4_2928 0.21 -0.2 3,284,776 - Pf1N1B4_2928 0.34 -0.2 3,284,776 - Pf1N1B4_2928 0.34 +1.4 3,284,776 - Pf1N1B4_2928 0.34 +0.8 3,284,776 - Pf1N1B4_2928 0.34 -0.8 3,284,800 - Pf1N1B4_2928 0.37 +0.8 3,284,800 - Pf1N1B4_2928 0.37 +0.8 3,284,847 + Pf1N1B4_2928 0.42 +0.8 3,284,847 + Pf1N1B4_2928 0.42 -1.1 3,284,848 - Pf1N1B4_2928 0.42 -0.8 3,284,883 + Pf1N1B4_2928 0.46 -1.6 3,284,883 + Pf1N1B4_2928 0.46 +0.2 3,284,960 + Pf1N1B4_2928 0.54 +0.8 3,284,961 - Pf1N1B4_2928 0.54 +1.1 3,284,961 - Pf1N1B4_2928 0.54 +0.8 3,284,961 - Pf1N1B4_2928 0.54 +0.8 3,284,961 - Pf1N1B4_2928 0.54 -2.1 3,284,961 - Pf1N1B4_2928 0.54 +0.8 3,284,961 - Pf1N1B4_2928 0.54 -1.3 3,284,961 - Pf1N1B4_2928 0.54 +0.8 3,285,008 + Pf1N1B4_2928 0.59 +0.8 3,285,009 - Pf1N1B4_2928 0.59 -1.3 3,285,009 - Pf1N1B4_2928 0.59 +0.8 3,285,167 - Pf1N1B4_2928 0.76 -2.2 3,285,167 - Pf1N1B4_2928 0.76 -1.1 3,285,226 + Pf1N1B4_2928 0.82 +1.9 3,285,227 - Pf1N1B4_2928 0.82 -0.0 3,285,395 + -1.6 3,285,441 - -2.6 3,285,441 - +0.8 3,285,459 - +0.8 3,285,483 - -1.2 3,285,483 - -2.4 3,285,740 + Pf1N1B4_2929 0.37 +0.8 3,285,741 - Pf1N1B4_2929 0.37 +0.8 3,285,741 - Pf1N1B4_2929 0.37 -0.1 3,285,741 - Pf1N1B4_2929 0.37 +0.3 3,285,771 - Pf1N1B4_2929 0.40 -1.0 3,285,771 - Pf1N1B4_2929 0.40 -2.0 3,285,771 - Pf1N1B4_2929 0.40 -2.0 3,285,771 - Pf1N1B4_2929 0.40 +0.8 3,285,777 - Pf1N1B4_2929 0.41 +0.8 3,285,954 + Pf1N1B4_2929 0.59 -0.2 3,285,955 - Pf1N1B4_2929 0.59 +1.4 3,286,039 - Pf1N1B4_2929 0.68 -3.0 3,286,044 + +0.1 3,286,045 - Pf1N1B4_2929 0.69 -0.3 3,286,045 - Pf1N1B4_2929 0.69 +0.4 3,286,045 - Pf1N1B4_2929 0.69 -0.2 3,286,045 - Pf1N1B4_2929 0.69 -0.9 3,286,047 - +0.8 3,286,236 - Pf1N1B4_2929 0.89 +0.9 3,286,287 - -0.4 3,286,306 + -0.8 3,286,344 + -1.6 3,286,379 - +0.1 3,286,385 + +0.8 3,286,386 - -1.4 3,286,388 + -0.6 3,286,389 - -2.9 3,286,389 - +0.8 3,286,397 - -0.1 3,286,414 + -2.3 3,286,414 + +0.8 3,286,415 - +0.8 3,286,415 - -0.8 3,286,428 + +0.3 3,286,429 - -0.4 3,286,590 + +0.8 3,286,590 + +0.8 3,286,590 + -0.2 3,286,590 + +0.8 3,286,590 + -0.6 3,286,590 + +1.0 3,286,590 + -1.9 3,286,590 + +0.8 3,286,590 + +0.1 3,286,591 - +1.8 3,286,591 - +0.8 3,286,591 - -3.7 3,286,591 - -2.3 3,286,591 - -2.4 3,286,591 - +0.8 3,286,591 - -1.8 3,286,591 - +1.4 3,286,591 - -1.1 3,286,591 - +0.8 3,286,943 + Pf1N1B4_2930 0.45 -0.6 3,286,944 - Pf1N1B4_2930 0.45 +0.8 3,287,224 + Pf1N1B4_2930 0.74 +0.8 3,287,224 + Pf1N1B4_2930 0.74 +0.1 3,287,224 + Pf1N1B4_2930 0.74 -0.6 3,287,224 + Pf1N1B4_2930 0.74 -1.6 3,287,225 - Pf1N1B4_2930 0.74 +0.8 3,287,225 - Pf1N1B4_2930 0.74 -0.3 3,287,225 - Pf1N1B4_2930 0.74 -2.1 3,287,226 + Pf1N1B4_2930 0.74 +0.8 3,287,226 + Pf1N1B4_2930 0.74 +0.8 3,287,226 + +0.8 3,287,226 + -1.0 3,287,226 + Pf1N1B4_2930 0.74 +0.8 3,287,227 - Pf1N1B4_2930 0.74 +0.8 3,287,227 - Pf1N1B4_2930 0.74 +0.3 3,287,227 - Pf1N1B4_2930 0.74 -2.2 3,287,227 - Pf1N1B4_2930 0.74 +0.8 3,287,227 - Pf1N1B4_2930 0.74 -2.7 3,287,313 + Pf1N1B4_2930 0.83 -1.6
Or see this region's nucleotide sequence