Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2867 and Pf1N1B4_2868 overlap by 1 nucleotides Pf1N1B4_2868 and Pf1N1B4_2869 are separated by 21 nucleotides
Pf1N1B4_2867 - Hemin ABC transporter, permease protein, at 3,214,644 to 3,215,681
_2867
Pf1N1B4_2868 - ABC-type hemin transport system, ATPase component, at 3,215,681 to 3,216,448
_2868
Pf1N1B4_2869 - lipoprotein, putative, at 3,216,470 to 3,217,336
_2869
Position (kb)
3215
3216
3217 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3214.754 kb on + strand, within Pf1N1B4_2867 at 3214.912 kb on + strand, within Pf1N1B4_2867 at 3214.912 kb on + strand, within Pf1N1B4_2867 at 3214.912 kb on + strand, within Pf1N1B4_2867 at 3214.913 kb on - strand, within Pf1N1B4_2867 at 3215.074 kb on + strand, within Pf1N1B4_2867 at 3215.074 kb on + strand, within Pf1N1B4_2867 at 3215.088 kb on + strand, within Pf1N1B4_2867 at 3215.190 kb on + strand, within Pf1N1B4_2867 at 3215.190 kb on + strand, within Pf1N1B4_2867 at 3215.190 kb on + strand, within Pf1N1B4_2867 at 3215.259 kb on + strand, within Pf1N1B4_2867 at 3215.260 kb on - strand, within Pf1N1B4_2867 at 3215.284 kb on + strand, within Pf1N1B4_2867 at 3215.656 kb on - strand at 3215.659 kb on + strand at 3215.659 kb on + strand at 3215.659 kb on + strand at 3215.659 kb on + strand at 3215.659 kb on + strand at 3215.659 kb on + strand at 3215.660 kb on - strand at 3215.660 kb on - strand at 3215.660 kb on - strand at 3215.660 kb on - strand at 3215.660 kb on - strand at 3215.660 kb on - strand at 3215.660 kb on - strand at 3215.660 kb on - strand at 3216.071 kb on + strand, within Pf1N1B4_2868 at 3216.072 kb on - strand, within Pf1N1B4_2868 at 3216.291 kb on - strand, within Pf1N1B4_2868 at 3216.303 kb on + strand, within Pf1N1B4_2868 at 3216.304 kb on - strand, within Pf1N1B4_2868 at 3216.306 kb on + strand, within Pf1N1B4_2868 at 3216.306 kb on + strand, within Pf1N1B4_2868 at 3216.306 kb on + strand, within Pf1N1B4_2868 at 3216.307 kb on - strand, within Pf1N1B4_2868 at 3216.307 kb on - strand, within Pf1N1B4_2868 at 3216.307 kb on - strand, within Pf1N1B4_2868 at 3216.340 kb on + strand, within Pf1N1B4_2868 at 3216.397 kb on - strand at 3216.397 kb on - strand at 3216.682 kb on - strand, within Pf1N1B4_2869 at 3216.682 kb on - strand, within Pf1N1B4_2869 at 3216.684 kb on + strand, within Pf1N1B4_2869 at 3216.684 kb on + strand, within Pf1N1B4_2869 at 3216.684 kb on + strand, within Pf1N1B4_2869 at 3216.684 kb on + strand, within Pf1N1B4_2869 at 3216.684 kb on + strand, within Pf1N1B4_2869 at 3216.685 kb on - strand, within Pf1N1B4_2869 at 3216.720 kb on + strand, within Pf1N1B4_2869 at 3216.720 kb on + strand, within Pf1N1B4_2869 at 3216.720 kb on + strand, within Pf1N1B4_2869 at 3216.842 kb on + strand, within Pf1N1B4_2869 at 3216.919 kb on + strand, within Pf1N1B4_2869 at 3217.092 kb on + strand, within Pf1N1B4_2869 at 3217.133 kb on + strand, within Pf1N1B4_2869 at 3217.203 kb on + strand, within Pf1N1B4_2869 at 3217.204 kb on - strand, within Pf1N1B4_2869 at 3217.253 kb on + strand at 3217.254 kb on - strand at 3217.254 kb on - strand at 3217.263 kb on - strand at 3217.263 kb on - strand at 3217.263 kb on - strand at 3217.264 kb on + strand at 3217.264 kb on + strand at 3217.264 kb on + strand at 3217.289 kb on + strand at 3217.307 kb on - strand at 3217.334 kb on + strand at 3217.334 kb on + strand at 3217.335 kb on - strand at 3217.391 kb on - strand at 3217.391 kb on - strand at 3217.401 kb on - strand at 3217.417 kb on - strand at 3217.417 kb on - strand at 3217.417 kb on - strand at 3217.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 3,214,754 + Pf1N1B4_2867 0.11 +0.1 3,214,912 + Pf1N1B4_2867 0.26 -2.6 3,214,912 + Pf1N1B4_2867 0.26 -0.2 3,214,912 + Pf1N1B4_2867 0.26 -1.5 3,214,913 - Pf1N1B4_2867 0.26 +0.1 3,215,074 + Pf1N1B4_2867 0.41 +0.8 3,215,074 + Pf1N1B4_2867 0.41 +0.4 3,215,088 + Pf1N1B4_2867 0.43 -1.8 3,215,190 + Pf1N1B4_2867 0.53 +0.8 3,215,190 + Pf1N1B4_2867 0.53 -2.0 3,215,190 + Pf1N1B4_2867 0.53 +0.4 3,215,259 + Pf1N1B4_2867 0.59 +0.8 3,215,260 - Pf1N1B4_2867 0.59 +0.8 3,215,284 + Pf1N1B4_2867 0.62 -0.3 3,215,656 - +0.8 3,215,659 + -1.0 3,215,659 + -1.6 3,215,659 + +1.0 3,215,659 + -2.2 3,215,659 + -1.4 3,215,659 + -1.0 3,215,660 - -1.8 3,215,660 - +1.8 3,215,660 - -0.6 3,215,660 - +0.8 3,215,660 - +0.8 3,215,660 - +0.8 3,215,660 - -0.8 3,215,660 - -1.1 3,216,071 + Pf1N1B4_2868 0.51 -1.7 3,216,072 - Pf1N1B4_2868 0.51 -0.7 3,216,291 - Pf1N1B4_2868 0.79 -2.6 3,216,303 + Pf1N1B4_2868 0.81 -0.9 3,216,304 - Pf1N1B4_2868 0.81 +0.8 3,216,306 + Pf1N1B4_2868 0.81 -2.2 3,216,306 + Pf1N1B4_2868 0.81 -1.9 3,216,306 + Pf1N1B4_2868 0.81 -3.6 3,216,307 - Pf1N1B4_2868 0.82 -2.6 3,216,307 - Pf1N1B4_2868 0.82 -1.1 3,216,307 - Pf1N1B4_2868 0.82 -1.8 3,216,340 + Pf1N1B4_2868 0.86 -1.0 3,216,397 - +0.4 3,216,397 - -2.0 3,216,682 - Pf1N1B4_2869 0.24 +0.8 3,216,682 - Pf1N1B4_2869 0.24 +0.4 3,216,684 + Pf1N1B4_2869 0.25 +0.8 3,216,684 + Pf1N1B4_2869 0.25 +2.3 3,216,684 + Pf1N1B4_2869 0.25 +0.8 3,216,684 + Pf1N1B4_2869 0.25 -4.0 3,216,684 + Pf1N1B4_2869 0.25 -0.7 3,216,685 - Pf1N1B4_2869 0.25 +0.1 3,216,720 + Pf1N1B4_2869 0.29 +0.8 3,216,720 + Pf1N1B4_2869 0.29 -3.3 3,216,720 + Pf1N1B4_2869 0.29 -1.2 3,216,842 + Pf1N1B4_2869 0.43 +0.8 3,216,919 + Pf1N1B4_2869 0.52 +1.5 3,217,092 + Pf1N1B4_2869 0.72 -0.5 3,217,133 + Pf1N1B4_2869 0.76 +0.8 3,217,203 + Pf1N1B4_2869 0.85 -2.2 3,217,204 - Pf1N1B4_2869 0.85 -2.2 3,217,253 + -0.8 3,217,254 - -0.5 3,217,254 - -0.9 3,217,263 - +0.8 3,217,263 - +0.8 3,217,263 - +0.8 3,217,264 + -4.0 3,217,264 + +0.8 3,217,264 + -2.3 3,217,289 + +0.8 3,217,307 - +0.8 3,217,334 + -2.9 3,217,334 + -0.1 3,217,335 - -2.4 3,217,391 - +0.8 3,217,391 - +0.8 3,217,401 - -0.1 3,217,417 - +0.8 3,217,417 - -1.2 3,217,417 - -0.5 3,217,417 - -0.3
Or see this region's nucleotide sequence