Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2754 and Pf1N1B4_2755 overlap by 4 nucleotides Pf1N1B4_2755 and Pf1N1B4_2756 are separated by 227 nucleotides Pf1N1B4_2756 and Pf1N1B4_2757 are separated by 67 nucleotides
Pf1N1B4_2754 - tRNA (Uracil54-C5-)-methyltransferase (EC 2.1.1.35), at 3,092,635 to 3,093,714
_2754
Pf1N1B4_2755 - Xanthine/uracil/thiamine/ascorbate permease family protein, at 3,093,711 to 3,095,006
_2755
Pf1N1B4_2756 - FIG00960037: hypothetical protein, at 3,095,234 to 3,095,689
_2756
Pf1N1B4_2757 - DJ-1/YajL/PfpI superfamily, includes chaperone protein YajL (former ThiJ), parkinsonism-associated protein DJ-1, peptidases PfpI, Hsp31, at 3,095,757 to 3,096,308
_2757
Position (kb)
3093
3094
3095
3096 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3092.774 kb on + strand, within Pf1N1B4_2754 at 3092.774 kb on + strand, within Pf1N1B4_2754 at 3092.775 kb on - strand, within Pf1N1B4_2754 at 3092.775 kb on - strand at 3092.864 kb on + strand, within Pf1N1B4_2754 at 3093.083 kb on + strand, within Pf1N1B4_2754 at 3093.083 kb on + strand, within Pf1N1B4_2754 at 3093.084 kb on - strand, within Pf1N1B4_2754 at 3093.084 kb on - strand, within Pf1N1B4_2754 at 3093.084 kb on - strand at 3093.084 kb on - strand, within Pf1N1B4_2754 at 3093.125 kb on + strand, within Pf1N1B4_2754 at 3093.125 kb on + strand, within Pf1N1B4_2754 at 3093.125 kb on + strand, within Pf1N1B4_2754 at 3093.126 kb on - strand, within Pf1N1B4_2754 at 3093.126 kb on - strand, within Pf1N1B4_2754 at 3093.214 kb on + strand, within Pf1N1B4_2754 at 3093.214 kb on + strand, within Pf1N1B4_2754 at 3093.214 kb on + strand, within Pf1N1B4_2754 at 3093.236 kb on + strand, within Pf1N1B4_2754 at 3093.236 kb on + strand, within Pf1N1B4_2754 at 3093.237 kb on - strand, within Pf1N1B4_2754 at 3093.351 kb on - strand, within Pf1N1B4_2754 at 3093.853 kb on + strand, within Pf1N1B4_2755 at 3093.853 kb on + strand, within Pf1N1B4_2755 at 3093.853 kb on + strand, within Pf1N1B4_2755 at 3093.853 kb on + strand, within Pf1N1B4_2755 at 3093.854 kb on - strand, within Pf1N1B4_2755 at 3093.854 kb on - strand, within Pf1N1B4_2755 at 3093.854 kb on - strand, within Pf1N1B4_2755 at 3093.854 kb on - strand at 3093.918 kb on + strand, within Pf1N1B4_2755 at 3093.919 kb on - strand at 3094.099 kb on + strand, within Pf1N1B4_2755 at 3094.099 kb on + strand, within Pf1N1B4_2755 at 3094.099 kb on + strand, within Pf1N1B4_2755 at 3094.099 kb on + strand, within Pf1N1B4_2755 at 3094.099 kb on + strand, within Pf1N1B4_2755 at 3094.099 kb on + strand, within Pf1N1B4_2755 at 3094.099 kb on + strand, within Pf1N1B4_2755 at 3094.100 kb on - strand at 3094.119 kb on - strand, within Pf1N1B4_2755 at 3094.539 kb on - strand, within Pf1N1B4_2755 at 3094.614 kb on + strand, within Pf1N1B4_2755 at 3094.747 kb on + strand, within Pf1N1B4_2755 at 3094.747 kb on + strand, within Pf1N1B4_2755 at 3094.748 kb on - strand, within Pf1N1B4_2755 at 3094.748 kb on - strand, within Pf1N1B4_2755 at 3094.748 kb on - strand, within Pf1N1B4_2755 at 3094.748 kb on - strand, within Pf1N1B4_2755 at 3094.915 kb on + strand at 3094.915 kb on + strand at 3094.915 kb on + strand at 3094.915 kb on + strand at 3094.915 kb on + strand at 3094.916 kb on - strand at 3094.916 kb on - strand at 3094.916 kb on - strand at 3094.916 kb on - strand at 3094.916 kb on - strand at 3094.916 kb on - strand at 3094.916 kb on - strand at 3094.916 kb on - strand at 3095.042 kb on + strand at 3095.120 kb on + strand at 3095.120 kb on + strand at 3095.120 kb on + strand at 3095.147 kb on + strand at 3095.148 kb on - strand at 3095.148 kb on - strand at 3095.148 kb on - strand at 3095.177 kb on + strand at 3095.178 kb on - strand at 3095.178 kb on - strand at 3095.230 kb on - strand at 3095.230 kb on - strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.233 kb on + strand at 3095.234 kb on - strand at 3095.234 kb on - strand at 3095.234 kb on - strand at 3095.234 kb on - strand at 3095.234 kb on - strand at 3095.234 kb on - strand at 3095.234 kb on - strand at 3095.234 kb on - strand at 3095.234 kb on - strand at 3095.234 kb on - strand at 3095.237 kb on + strand at 3095.237 kb on + strand at 3095.237 kb on + strand at 3095.237 kb on + strand at 3095.237 kb on + strand at 3095.237 kb on + strand at 3095.237 kb on + strand at 3095.237 kb on + strand at 3095.237 kb on + strand at 3095.238 kb on - strand at 3095.238 kb on - strand at 3095.238 kb on - strand at 3095.238 kb on - strand at 3095.245 kb on + strand at 3095.248 kb on + strand at 3095.249 kb on - strand at 3095.383 kb on + strand, within Pf1N1B4_2756 at 3095.441 kb on + strand, within Pf1N1B4_2756 at 3095.441 kb on + strand, within Pf1N1B4_2756 at 3095.441 kb on + strand, within Pf1N1B4_2756 at 3095.441 kb on + strand, within Pf1N1B4_2756 at 3095.441 kb on + strand, within Pf1N1B4_2756 at 3095.441 kb on + strand, within Pf1N1B4_2756 at 3095.441 kb on + strand, within Pf1N1B4_2756 at 3095.441 kb on + strand, within Pf1N1B4_2756 at 3095.441 kb on + strand, within Pf1N1B4_2756 at 3095.441 kb on + strand, within Pf1N1B4_2756 at 3095.441 kb on + strand, within Pf1N1B4_2756 at 3095.441 kb on + strand, within Pf1N1B4_2756 at 3095.441 kb on + strand, within Pf1N1B4_2756 at 3095.442 kb on - strand, within Pf1N1B4_2756 at 3095.442 kb on - strand at 3095.442 kb on - strand, within Pf1N1B4_2756 at 3095.442 kb on - strand at 3095.442 kb on - strand, within Pf1N1B4_2756 at 3095.442 kb on - strand, within Pf1N1B4_2756 at 3095.442 kb on - strand, within Pf1N1B4_2756 at 3095.442 kb on - strand, within Pf1N1B4_2756 at 3095.442 kb on - strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.585 kb on + strand, within Pf1N1B4_2756 at 3095.586 kb on - strand, within Pf1N1B4_2756 at 3095.586 kb on - strand, within Pf1N1B4_2756 at 3095.586 kb on - strand, within Pf1N1B4_2756 at 3095.586 kb on - strand, within Pf1N1B4_2756 at 3095.635 kb on + strand, within Pf1N1B4_2756 at 3095.635 kb on + strand at 3095.636 kb on - strand, within Pf1N1B4_2756 at 3095.636 kb on - strand, within Pf1N1B4_2756 at 3095.678 kb on + strand at 3095.678 kb on + strand at 3095.678 kb on + strand at 3095.678 kb on + strand at 3095.679 kb on - strand at 3095.679 kb on - strand at 3095.679 kb on - strand at 3095.679 kb on - strand at 3095.679 kb on - strand at 3095.679 kb on - strand at 3095.725 kb on - strand at 3095.765 kb on + strand at 3095.879 kb on + strand, within Pf1N1B4_2757 at 3095.879 kb on + strand, within Pf1N1B4_2757 at 3095.879 kb on + strand, within Pf1N1B4_2757 at 3095.879 kb on + strand, within Pf1N1B4_2757 at 3095.879 kb on + strand, within Pf1N1B4_2757 at 3095.880 kb on - strand, within Pf1N1B4_2757 at 3095.880 kb on - strand, within Pf1N1B4_2757 at 3095.880 kb on - strand, within Pf1N1B4_2757 at 3095.880 kb on - strand, within Pf1N1B4_2757 at 3095.936 kb on - strand, within Pf1N1B4_2757
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 3,092,774 + Pf1N1B4_2754 0.13 +0.1 3,092,774 + Pf1N1B4_2754 0.13 +0.8 3,092,775 - Pf1N1B4_2754 0.13 +0.0 3,092,775 - +0.8 3,092,864 + Pf1N1B4_2754 0.21 +0.8 3,093,083 + Pf1N1B4_2754 0.41 -1.2 3,093,083 + Pf1N1B4_2754 0.41 -1.2 3,093,084 - Pf1N1B4_2754 0.42 -2.1 3,093,084 - Pf1N1B4_2754 0.42 +0.8 3,093,084 - -2.3 3,093,084 - Pf1N1B4_2754 0.42 -1.8 3,093,125 + Pf1N1B4_2754 0.45 -0.6 3,093,125 + Pf1N1B4_2754 0.45 +2.3 3,093,125 + Pf1N1B4_2754 0.45 -2.1 3,093,126 - Pf1N1B4_2754 0.45 +1.2 3,093,126 - Pf1N1B4_2754 0.45 +0.8 3,093,214 + Pf1N1B4_2754 0.54 -2.7 3,093,214 + Pf1N1B4_2754 0.54 -1.2 3,093,214 + Pf1N1B4_2754 0.54 -0.5 3,093,236 + Pf1N1B4_2754 0.56 -1.8 3,093,236 + Pf1N1B4_2754 0.56 -0.4 3,093,237 - Pf1N1B4_2754 0.56 -0.8 3,093,351 - Pf1N1B4_2754 0.66 -1.1 3,093,853 + Pf1N1B4_2755 0.11 -3.3 3,093,853 + Pf1N1B4_2755 0.11 +0.8 3,093,853 + Pf1N1B4_2755 0.11 -0.8 3,093,853 + Pf1N1B4_2755 0.11 -2.4 3,093,854 - Pf1N1B4_2755 0.11 -2.2 3,093,854 - Pf1N1B4_2755 0.11 -1.1 3,093,854 - Pf1N1B4_2755 0.11 +0.8 3,093,854 - +0.8 3,093,918 + Pf1N1B4_2755 0.16 -1.0 3,093,919 - -3.3 3,094,099 + Pf1N1B4_2755 0.30 +0.8 3,094,099 + Pf1N1B4_2755 0.30 +0.8 3,094,099 + Pf1N1B4_2755 0.30 +0.8 3,094,099 + Pf1N1B4_2755 0.30 +0.8 3,094,099 + Pf1N1B4_2755 0.30 -1.1 3,094,099 + Pf1N1B4_2755 0.30 +0.8 3,094,099 + Pf1N1B4_2755 0.30 +0.8 3,094,100 - -0.8 3,094,119 - Pf1N1B4_2755 0.31 -3.1 3,094,539 - Pf1N1B4_2755 0.64 -3.2 3,094,614 + Pf1N1B4_2755 0.70 +0.0 3,094,747 + Pf1N1B4_2755 0.80 -1.6 3,094,747 + Pf1N1B4_2755 0.80 -2.1 3,094,748 - Pf1N1B4_2755 0.80 +0.8 3,094,748 - Pf1N1B4_2755 0.80 -0.2 3,094,748 - Pf1N1B4_2755 0.80 -3.2 3,094,748 - Pf1N1B4_2755 0.80 +0.8 3,094,915 + -2.1 3,094,915 + +0.8 3,094,915 + -1.6 3,094,915 + -1.4 3,094,915 + +2.1 3,094,916 - -0.9 3,094,916 - +0.8 3,094,916 - +0.8 3,094,916 - +0.8 3,094,916 - +1.5 3,094,916 - -2.6 3,094,916 - +2.6 3,094,916 - +0.8 3,095,042 + +0.3 3,095,120 + +0.8 3,095,120 + +0.3 3,095,120 + +0.8 3,095,147 + -0.2 3,095,148 - -1.7 3,095,148 - -3.3 3,095,148 - -1.2 3,095,177 + -2.4 3,095,178 - -2.8 3,095,178 - -0.7 3,095,230 - +2.1 3,095,230 - +0.2 3,095,233 + +0.8 3,095,233 + +0.8 3,095,233 + -2.1 3,095,233 + +0.8 3,095,233 + -0.7 3,095,233 + +0.8 3,095,233 + -3.4 3,095,233 + +0.8 3,095,233 + +0.6 3,095,233 + -1.2 3,095,233 + -0.2 3,095,233 + +1.0 3,095,233 + +0.8 3,095,233 + -0.7 3,095,233 + -0.2 3,095,233 + -0.5 3,095,233 + +0.4 3,095,234 - +0.8 3,095,234 - -0.9 3,095,234 - +0.8 3,095,234 - +0.8 3,095,234 - -0.1 3,095,234 - +0.3 3,095,234 - -1.4 3,095,234 - +0.3 3,095,234 - +0.8 3,095,234 - -0.7 3,095,237 + +0.8 3,095,237 + +0.8 3,095,237 + +0.8 3,095,237 + -1.8 3,095,237 + +0.5 3,095,237 + +0.8 3,095,237 + -2.5 3,095,237 + +0.3 3,095,237 + -1.8 3,095,238 - -1.8 3,095,238 - +0.8 3,095,238 - -0.8 3,095,238 - +0.5 3,095,245 + +0.8 3,095,248 + -1.6 3,095,249 - -0.8 3,095,383 + Pf1N1B4_2756 0.33 -0.1 3,095,441 + Pf1N1B4_2756 0.45 -0.7 3,095,441 + Pf1N1B4_2756 0.45 -2.4 3,095,441 + Pf1N1B4_2756 0.45 -1.8 3,095,441 + Pf1N1B4_2756 0.45 +0.6 3,095,441 + Pf1N1B4_2756 0.45 +0.8 3,095,441 + Pf1N1B4_2756 0.45 +0.5 3,095,441 + Pf1N1B4_2756 0.45 -2.6 3,095,441 + Pf1N1B4_2756 0.45 -2.7 3,095,441 + Pf1N1B4_2756 0.45 -1.7 3,095,441 + Pf1N1B4_2756 0.45 -1.1 3,095,441 + Pf1N1B4_2756 0.45 +0.9 3,095,441 + Pf1N1B4_2756 0.45 -1.6 3,095,441 + Pf1N1B4_2756 0.45 +0.4 3,095,442 - Pf1N1B4_2756 0.46 -0.7 3,095,442 - +0.8 3,095,442 - Pf1N1B4_2756 0.46 +0.2 3,095,442 - -0.3 3,095,442 - Pf1N1B4_2756 0.46 +0.8 3,095,442 - Pf1N1B4_2756 0.46 -0.2 3,095,442 - Pf1N1B4_2756 0.46 +0.8 3,095,442 - Pf1N1B4_2756 0.46 +0.8 3,095,442 - Pf1N1B4_2756 0.46 +0.9 3,095,585 + Pf1N1B4_2756 0.77 +0.8 3,095,585 + Pf1N1B4_2756 0.77 -1.6 3,095,585 + Pf1N1B4_2756 0.77 +0.8 3,095,585 + Pf1N1B4_2756 0.77 -1.0 3,095,585 + Pf1N1B4_2756 0.77 +0.8 3,095,585 + Pf1N1B4_2756 0.77 +0.4 3,095,585 + Pf1N1B4_2756 0.77 -1.3 3,095,585 + Pf1N1B4_2756 0.77 -0.8 3,095,585 + Pf1N1B4_2756 0.77 -2.4 3,095,585 + Pf1N1B4_2756 0.77 +0.8 3,095,585 + Pf1N1B4_2756 0.77 -0.3 3,095,585 + Pf1N1B4_2756 0.77 -2.7 3,095,585 + Pf1N1B4_2756 0.77 -2.2 3,095,585 + Pf1N1B4_2756 0.77 +0.8 3,095,585 + Pf1N1B4_2756 0.77 -0.2 3,095,586 - Pf1N1B4_2756 0.77 -0.8 3,095,586 - Pf1N1B4_2756 0.77 +0.8 3,095,586 - Pf1N1B4_2756 0.77 -0.2 3,095,586 - Pf1N1B4_2756 0.77 -2.5 3,095,635 + Pf1N1B4_2756 0.88 -0.8 3,095,635 + +0.8 3,095,636 - Pf1N1B4_2756 0.88 -3.4 3,095,636 - Pf1N1B4_2756 0.88 -2.7 3,095,678 + +0.8 3,095,678 + +0.3 3,095,678 + +0.2 3,095,678 + +0.0 3,095,679 - -0.2 3,095,679 - -0.1 3,095,679 - -2.6 3,095,679 - -0.2 3,095,679 - -1.2 3,095,679 - +0.5 3,095,725 - +0.8 3,095,765 + +0.8 3,095,879 + Pf1N1B4_2757 0.22 +0.8 3,095,879 + Pf1N1B4_2757 0.22 +0.8 3,095,879 + Pf1N1B4_2757 0.22 -1.7 3,095,879 + Pf1N1B4_2757 0.22 -0.8 3,095,879 + Pf1N1B4_2757 0.22 -0.2 3,095,880 - Pf1N1B4_2757 0.22 -0.3 3,095,880 - Pf1N1B4_2757 0.22 -4.2 3,095,880 - Pf1N1B4_2757 0.22 +0.8 3,095,880 - Pf1N1B4_2757 0.22 -2.2 3,095,936 - Pf1N1B4_2757 0.32 +0.8
Or see this region's nucleotide sequence