Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2464

Experiment: N2E2wt with N1B4m denitrifying repB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2463 and Pf1N1B4_2464 are separated by 273 nucleotidesPf1N1B4_2464 and Pf1N1B4_2465 are separated by 59 nucleotidesPf1N1B4_2465 and Pf1N1B4_2466 overlap by 1 nucleotides Pf1N1B4_2463 - Acyltransferase family protein, at 2,793,569 to 2,794,453 _2463 Pf1N1B4_2464 - Ribosomal protein S6 glutaminyl transferase, at 2,794,727 to 2,795,233 _2464 Pf1N1B4_2465 - Two-component response regulator CreB, at 2,795,293 to 2,795,973 _2465 Pf1N1B4_2466 - Two-component response regulator CreC, at 2,795,973 to 2,797,406 _2466 Position (kb) 2794 2795 2796Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2793.912 kb on + strand, within Pf1N1B4_2463at 2793.930 kb on + strand, within Pf1N1B4_2463at 2793.931 kb on - strand, within Pf1N1B4_2463at 2793.931 kb on - strand, within Pf1N1B4_2463at 2793.931 kb on - strand, within Pf1N1B4_2463at 2793.931 kb on - strand, within Pf1N1B4_2463at 2793.960 kb on + strand, within Pf1N1B4_2463at 2793.960 kb on + strand, within Pf1N1B4_2463at 2793.960 kb on + strand, within Pf1N1B4_2463at 2793.961 kb on - strand, within Pf1N1B4_2463at 2793.961 kb on - strand, within Pf1N1B4_2463at 2793.961 kb on - strand, within Pf1N1B4_2463at 2793.984 kb on - strand, within Pf1N1B4_2463at 2794.057 kb on - strand, within Pf1N1B4_2463at 2794.057 kb on - strand, within Pf1N1B4_2463at 2794.203 kb on + strand, within Pf1N1B4_2463at 2794.203 kb on + strand, within Pf1N1B4_2463at 2794.203 kb on + strand, within Pf1N1B4_2463at 2794.203 kb on + strandat 2794.203 kb on + strand, within Pf1N1B4_2463at 2794.204 kb on - strand, within Pf1N1B4_2463at 2794.204 kb on - strand, within Pf1N1B4_2463at 2794.204 kb on - strand, within Pf1N1B4_2463at 2794.329 kb on + strand, within Pf1N1B4_2463at 2794.329 kb on + strand, within Pf1N1B4_2463at 2794.330 kb on - strand, within Pf1N1B4_2463at 2794.453 kb on + strandat 2794.453 kb on + strandat 2794.453 kb on + strandat 2794.453 kb on + strandat 2794.453 kb on + strandat 2794.453 kb on + strandat 2794.454 kb on - strandat 2794.454 kb on - strandat 2794.454 kb on - strandat 2794.454 kb on - strandat 2794.488 kb on + strandat 2794.489 kb on - strandat 2794.489 kb on - strandat 2794.563 kb on - strandat 2794.690 kb on - strandat 2794.725 kb on - strandat 2794.809 kb on - strand, within Pf1N1B4_2464at 2794.809 kb on - strand, within Pf1N1B4_2464at 2794.809 kb on - strand, within Pf1N1B4_2464at 2794.970 kb on + strand, within Pf1N1B4_2464at 2794.970 kb on + strand, within Pf1N1B4_2464at 2794.970 kb on + strand, within Pf1N1B4_2464at 2794.971 kb on - strand, within Pf1N1B4_2464at 2794.971 kb on - strand, within Pf1N1B4_2464at 2795.101 kb on + strand, within Pf1N1B4_2464at 2795.101 kb on + strand, within Pf1N1B4_2464at 2795.272 kb on + strandat 2795.272 kb on + strandat 2795.273 kb on - strandat 2795.273 kb on - strandat 2795.273 kb on - strandat 2795.273 kb on - strandat 2795.273 kb on - strandat 2795.276 kb on + strandat 2795.371 kb on - strand, within Pf1N1B4_2465at 2795.584 kb on + strand, within Pf1N1B4_2465at 2795.584 kb on + strand, within Pf1N1B4_2465at 2795.719 kb on + strand, within Pf1N1B4_2465at 2795.719 kb on + strand, within Pf1N1B4_2465at 2795.953 kb on + strandat 2795.954 kb on - strandat 2795.954 kb on - strandat 2796.003 kb on + strandat 2796.003 kb on + strandat 2796.003 kb on + strandat 2796.003 kb on + strandat 2796.003 kb on + strandat 2796.004 kb on - strandat 2796.004 kb on - strandat 2796.004 kb on - strandat 2796.192 kb on + strand, within Pf1N1B4_2466at 2796.218 kb on + strand, within Pf1N1B4_2466at 2796.218 kb on + strand, within Pf1N1B4_2466at 2796.219 kb on - strand, within Pf1N1B4_2466at 2796.219 kb on - strand, within Pf1N1B4_2466at 2796.219 kb on - strand, within Pf1N1B4_2466

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
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2,793,912 + Pf1N1B4_2463 0.39 +0.8
2,793,930 + Pf1N1B4_2463 0.41 +0.8
2,793,931 - Pf1N1B4_2463 0.41 -1.2
2,793,931 - Pf1N1B4_2463 0.41 -3.1
2,793,931 - Pf1N1B4_2463 0.41 -0.4
2,793,931 - Pf1N1B4_2463 0.41 -2.7
2,793,960 + Pf1N1B4_2463 0.44 -0.0
2,793,960 + Pf1N1B4_2463 0.44 +0.8
2,793,960 + Pf1N1B4_2463 0.44 -2.9
2,793,961 - Pf1N1B4_2463 0.44 +1.1
2,793,961 - Pf1N1B4_2463 0.44 -0.8
2,793,961 - Pf1N1B4_2463 0.44 -1.5
2,793,984 - Pf1N1B4_2463 0.47 +0.8
2,794,057 - Pf1N1B4_2463 0.55 +0.4
2,794,057 - Pf1N1B4_2463 0.55 +0.8
2,794,203 + Pf1N1B4_2463 0.72 -0.3
2,794,203 + Pf1N1B4_2463 0.72 -2.2
2,794,203 + Pf1N1B4_2463 0.72 +1.7
2,794,203 + -0.7
2,794,203 + Pf1N1B4_2463 0.72 +0.8
2,794,204 - Pf1N1B4_2463 0.72 -2.0
2,794,204 - Pf1N1B4_2463 0.72 +0.8
2,794,204 - Pf1N1B4_2463 0.72 -3.0
2,794,329 + Pf1N1B4_2463 0.86 +0.8
2,794,329 + Pf1N1B4_2463 0.86 +0.8
2,794,330 - Pf1N1B4_2463 0.86 +0.8
2,794,453 + +0.8
2,794,453 + +0.1
2,794,453 + +2.2
2,794,453 + +0.8
2,794,453 + -1.8
2,794,453 + -1.5
2,794,454 - +0.1
2,794,454 - +2.3
2,794,454 - +0.8
2,794,454 - +0.5
2,794,488 + +0.8
2,794,489 - +0.8
2,794,489 - +0.8
2,794,563 - +0.1
2,794,690 - -1.8
2,794,725 - -0.2
2,794,809 - Pf1N1B4_2464 0.16 +1.2
2,794,809 - Pf1N1B4_2464 0.16 +0.8
2,794,809 - Pf1N1B4_2464 0.16 -3.0
2,794,970 + Pf1N1B4_2464 0.48 +0.8
2,794,970 + Pf1N1B4_2464 0.48 -2.7
2,794,970 + Pf1N1B4_2464 0.48 +0.8
2,794,971 - Pf1N1B4_2464 0.48 -2.1
2,794,971 - Pf1N1B4_2464 0.48 +0.8
2,795,101 + Pf1N1B4_2464 0.74 +0.8
2,795,101 + Pf1N1B4_2464 0.74 -2.8
2,795,272 + -1.0
2,795,272 + -1.8
2,795,273 - -0.8
2,795,273 - +0.8
2,795,273 - -1.1
2,795,273 - -0.2
2,795,273 - -0.8
2,795,276 + +0.8
2,795,371 - Pf1N1B4_2465 0.11 -3.3
2,795,584 + Pf1N1B4_2465 0.43 +0.3
2,795,584 + Pf1N1B4_2465 0.43 +0.8
2,795,719 + Pf1N1B4_2465 0.63 -0.8
2,795,719 + Pf1N1B4_2465 0.63 -2.0
2,795,953 + +0.8
2,795,954 - +0.2
2,795,954 - -2.8
2,796,003 + -2.7
2,796,003 + +0.8
2,796,003 + +0.8
2,796,003 + -0.8
2,796,003 + +0.2
2,796,004 - +0.8
2,796,004 - -0.2
2,796,004 - +0.7
2,796,192 + Pf1N1B4_2466 0.15 +0.4
2,796,218 + Pf1N1B4_2466 0.17 -0.4
2,796,218 + Pf1N1B4_2466 0.17 -2.0
2,796,219 - Pf1N1B4_2466 0.17 -1.9
2,796,219 - Pf1N1B4_2466 0.17 -1.2
2,796,219 - Pf1N1B4_2466 0.17 +0.8

Or see this region's nucleotide sequence