Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2391 and Pf1N1B4_2392 are separated by 128 nucleotides Pf1N1B4_2392 and Pf1N1B4_2393 are separated by 42 nucleotides Pf1N1B4_2393 and Pf1N1B4_2394 are separated by 90 nucleotides
Pf1N1B4_2391 - Membrane-bound lytic murein transglycosylase A precursor (EC 3.2.1.-), at 2,708,719 to 2,709,891
_2391
Pf1N1B4_2392 - Cytochrome c556, at 2,710,020 to 2,710,469
_2392
Pf1N1B4_2393 - Protein yqjC precursor, at 2,710,512 to 2,710,907
_2393
Pf1N1B4_2394 - DNA ligase (EC 6.5.1.2), at 2,710,998 to 2,712,677
_2394
Position (kb)
2710
2711 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2709.065 kb on + strand, within Pf1N1B4_2391 at 2709.065 kb on + strand at 2709.066 kb on - strand at 2709.066 kb on - strand, within Pf1N1B4_2391 at 2709.066 kb on - strand, within Pf1N1B4_2391 at 2709.425 kb on + strand, within Pf1N1B4_2391 at 2709.425 kb on + strand, within Pf1N1B4_2391 at 2709.425 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.430 kb on + strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.470 kb on + strand, within Pf1N1B4_2391 at 2709.470 kb on + strand, within Pf1N1B4_2391 at 2709.470 kb on + strand, within Pf1N1B4_2391 at 2709.470 kb on + strand, within Pf1N1B4_2391 at 2709.471 kb on - strand, within Pf1N1B4_2391 at 2709.471 kb on - strand, within Pf1N1B4_2391 at 2709.471 kb on - strand, within Pf1N1B4_2391 at 2709.471 kb on - strand, within Pf1N1B4_2391 at 2709.527 kb on + strand, within Pf1N1B4_2391 at 2709.527 kb on + strand, within Pf1N1B4_2391 at 2709.527 kb on + strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.578 kb on + strand, within Pf1N1B4_2391 at 2709.579 kb on - strand, within Pf1N1B4_2391 at 2709.579 kb on - strand, within Pf1N1B4_2391 at 2709.687 kb on + strand, within Pf1N1B4_2391 at 2709.770 kb on + strand, within Pf1N1B4_2391 at 2709.770 kb on + strand, within Pf1N1B4_2391 at 2709.771 kb on - strand, within Pf1N1B4_2391 at 2709.896 kb on + strand at 2709.896 kb on + strand at 2709.897 kb on - strand at 2709.897 kb on - strand at 2709.897 kb on - strand at 2709.950 kb on + strand at 2709.950 kb on + strand at 2709.953 kb on + strand at 2709.953 kb on + strand at 2709.953 kb on + strand at 2709.953 kb on + strand at 2709.954 kb on - strand at 2709.954 kb on - strand at 2710.028 kb on + strand at 2710.029 kb on - strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.046 kb on - strand at 2710.046 kb on - strand at 2710.046 kb on - strand at 2710.046 kb on - strand at 2710.175 kb on + strand, within Pf1N1B4_2392 at 2710.175 kb on + strand, within Pf1N1B4_2392 at 2710.175 kb on + strand, within Pf1N1B4_2392 at 2710.176 kb on - strand, within Pf1N1B4_2392 at 2710.386 kb on + strand, within Pf1N1B4_2392 at 2710.386 kb on + strand, within Pf1N1B4_2392 at 2710.387 kb on - strand, within Pf1N1B4_2392 at 2710.387 kb on - strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.514 kb on - strand at 2710.514 kb on - strand at 2710.514 kb on - strand at 2710.517 kb on + strand at 2710.517 kb on + strand at 2710.517 kb on + strand at 2710.907 kb on + strand at 2710.907 kb on + strand at 2710.907 kb on + strand at 2710.907 kb on + strand at 2710.934 kb on + strand at 2710.934 kb on + strand at 2710.934 kb on + strand at 2710.934 kb on + strand at 2710.934 kb on + strand at 2710.934 kb on + strand at 2710.934 kb on + strand at 2710.934 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.026 kb on - strand at 2711.285 kb on + strand, within Pf1N1B4_2394 at 2711.285 kb on + strand, within Pf1N1B4_2394 at 2711.285 kb on + strand, within Pf1N1B4_2394
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 2,709,065 + Pf1N1B4_2391 0.29 +0.8 2,709,065 + +0.8 2,709,066 - +0.8 2,709,066 - Pf1N1B4_2391 0.30 -3.4 2,709,066 - Pf1N1B4_2391 0.30 -1.1 2,709,425 + Pf1N1B4_2391 0.60 -3.6 2,709,425 + Pf1N1B4_2391 0.60 -2.3 2,709,425 + Pf1N1B4_2391 0.60 -1.5 2,709,429 + Pf1N1B4_2391 0.61 +1.0 2,709,429 + Pf1N1B4_2391 0.61 +0.8 2,709,429 + Pf1N1B4_2391 0.61 -0.7 2,709,429 + Pf1N1B4_2391 0.61 -1.0 2,709,429 + Pf1N1B4_2391 0.61 -0.2 2,709,429 + Pf1N1B4_2391 0.61 -1.8 2,709,429 + Pf1N1B4_2391 0.61 -3.0 2,709,429 + Pf1N1B4_2391 0.61 +0.8 2,709,429 + Pf1N1B4_2391 0.61 +2.8 2,709,429 + Pf1N1B4_2391 0.61 -1.5 2,709,429 + +0.8 2,709,429 + Pf1N1B4_2391 0.61 +0.8 2,709,429 + Pf1N1B4_2391 0.61 +0.8 2,709,429 + Pf1N1B4_2391 0.61 -1.8 2,709,429 + Pf1N1B4_2391 0.61 +0.8 2,709,429 + Pf1N1B4_2391 0.61 -2.9 2,709,429 + Pf1N1B4_2391 0.61 -2.0 2,709,429 + Pf1N1B4_2391 0.61 -1.0 2,709,429 + Pf1N1B4_2391 0.61 -0.8 2,709,429 + Pf1N1B4_2391 0.61 -0.6 2,709,429 + Pf1N1B4_2391 0.61 -0.2 2,709,430 + Pf1N1B4_2391 0.61 -0.4 2,709,430 - Pf1N1B4_2391 0.61 -0.1 2,709,430 - Pf1N1B4_2391 0.61 -1.1 2,709,430 - Pf1N1B4_2391 0.61 +0.3 2,709,430 - Pf1N1B4_2391 0.61 +0.8 2,709,430 - Pf1N1B4_2391 0.61 -1.6 2,709,430 - Pf1N1B4_2391 0.61 +0.6 2,709,430 - Pf1N1B4_2391 0.61 -0.7 2,709,430 - Pf1N1B4_2391 0.61 +0.8 2,709,430 - Pf1N1B4_2391 0.61 -1.1 2,709,430 - Pf1N1B4_2391 0.61 -0.8 2,709,430 - Pf1N1B4_2391 0.61 -1.5 2,709,430 - Pf1N1B4_2391 0.61 +0.8 2,709,430 - Pf1N1B4_2391 0.61 +0.3 2,709,430 - Pf1N1B4_2391 0.61 +0.8 2,709,430 - Pf1N1B4_2391 0.61 -3.0 2,709,470 + Pf1N1B4_2391 0.64 +0.8 2,709,470 + Pf1N1B4_2391 0.64 -1.5 2,709,470 + Pf1N1B4_2391 0.64 +0.3 2,709,470 + Pf1N1B4_2391 0.64 -1.3 2,709,471 - Pf1N1B4_2391 0.64 -2.3 2,709,471 - Pf1N1B4_2391 0.64 -0.6 2,709,471 - Pf1N1B4_2391 0.64 +0.2 2,709,471 - Pf1N1B4_2391 0.64 +0.8 2,709,527 + Pf1N1B4_2391 0.69 -2.9 2,709,527 + Pf1N1B4_2391 0.69 -0.2 2,709,527 + Pf1N1B4_2391 0.69 +0.8 2,709,528 - Pf1N1B4_2391 0.69 +0.8 2,709,528 - Pf1N1B4_2391 0.69 -0.4 2,709,528 - Pf1N1B4_2391 0.69 +0.1 2,709,528 - Pf1N1B4_2391 0.69 -1.5 2,709,528 - Pf1N1B4_2391 0.69 +2.1 2,709,528 - Pf1N1B4_2391 0.69 +0.8 2,709,528 - Pf1N1B4_2391 0.69 +1.0 2,709,528 - Pf1N1B4_2391 0.69 +1.4 2,709,578 + Pf1N1B4_2391 0.73 -0.0 2,709,579 - Pf1N1B4_2391 0.73 +0.8 2,709,579 - Pf1N1B4_2391 0.73 -1.3 2,709,687 + Pf1N1B4_2391 0.83 -0.9 2,709,770 + Pf1N1B4_2391 0.90 +0.8 2,709,770 + Pf1N1B4_2391 0.90 -0.6 2,709,771 - Pf1N1B4_2391 0.90 -3.3 2,709,896 + +1.2 2,709,896 + +0.4 2,709,897 - +0.8 2,709,897 - -2.2 2,709,897 - -3.2 2,709,950 + +0.5 2,709,950 + -1.5 2,709,953 + +3.2 2,709,953 + +0.8 2,709,953 + +0.8 2,709,953 + -1.9 2,709,954 - +0.4 2,709,954 - -2.3 2,710,028 + +0.8 2,710,029 - -0.3 2,710,045 + -3.1 2,710,045 + -1.8 2,710,045 + -1.5 2,710,045 + +0.7 2,710,045 + -3.2 2,710,045 + -2.6 2,710,045 + -0.4 2,710,045 + -2.1 2,710,045 + +0.1 2,710,045 + -2.5 2,710,045 + +0.8 2,710,045 + -0.6 2,710,045 + -3.4 2,710,046 - -1.5 2,710,046 - -1.0 2,710,046 - +0.1 2,710,046 - +0.8 2,710,175 + Pf1N1B4_2392 0.34 -0.2 2,710,175 + Pf1N1B4_2392 0.34 -0.5 2,710,175 + Pf1N1B4_2392 0.34 +0.2 2,710,176 - Pf1N1B4_2392 0.35 +0.8 2,710,386 + Pf1N1B4_2392 0.81 +1.0 2,710,386 + Pf1N1B4_2392 0.81 -0.3 2,710,387 - Pf1N1B4_2392 0.82 +0.8 2,710,387 - +0.8 2,710,513 + +0.8 2,710,513 + -1.2 2,710,513 + -2.3 2,710,513 + -0.2 2,710,513 + +1.1 2,710,514 - -0.8 2,710,514 - -0.2 2,710,514 - -0.8 2,710,517 + +0.8 2,710,517 + -0.0 2,710,517 + +2.8 2,710,907 + -2.2 2,710,907 + +0.8 2,710,907 + +0.2 2,710,907 + -1.5 2,710,934 + -0.3 2,710,934 + -2.0 2,710,934 + +0.8 2,710,934 + +1.4 2,710,934 + -1.6 2,710,934 + +0.8 2,710,934 + -0.2 2,710,934 + -0.6 2,711,025 + -0.2 2,711,025 + -1.5 2,711,025 + +1.4 2,711,025 + -2.5 2,711,025 + +1.7 2,711,025 + +0.8 2,711,025 + -0.8 2,711,025 + +0.2 2,711,025 + -2.5 2,711,026 - -1.3 2,711,285 + Pf1N1B4_2394 0.17 +0.8 2,711,285 + Pf1N1B4_2394 0.17 -1.1 2,711,285 + Pf1N1B4_2394 0.17 -0.3
Or see this region's nucleotide sequence