Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2334

Experiment: N2E2wt with N1B4m denitrifying repB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2332 and Pf1N1B4_2333 are separated by 116 nucleotidesPf1N1B4_2333 and Pf1N1B4_2334 are separated by 9 nucleotidesPf1N1B4_2334 and Pf1N1B4_2335 overlap by 4 nucleotidesPf1N1B4_2335 and Pf1N1B4_2336 overlap by 4 nucleotides Pf1N1B4_2332 - tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33), at 2,650,671 to 2,651,396 _2332 Pf1N1B4_2333 - Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, at 2,651,513 to 2,651,836 _2333 Pf1N1B4_2334 - Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB, at 2,651,846 to 2,653,051 _2334 Pf1N1B4_2335 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15), at 2,653,048 to 2,653,644 _2335 Pf1N1B4_2336 - FIG003551: hypothetical protein, at 2,653,641 to 2,654,075 _2336 Position (kb) 2651 2652 2653 2654Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2651.029 kb on - strand, within Pf1N1B4_2332at 2651.235 kb on + strand, within Pf1N1B4_2332at 2651.235 kb on + strand, within Pf1N1B4_2332at 2651.235 kb on + strand, within Pf1N1B4_2332at 2651.394 kb on + strandat 2651.394 kb on + strandat 2651.504 kb on + strandat 2651.504 kb on + strandat 2651.505 kb on - strandat 2651.505 kb on - strandat 2651.577 kb on + strand, within Pf1N1B4_2333at 2651.577 kb on + strand, within Pf1N1B4_2333at 2651.577 kb on + strand, within Pf1N1B4_2333at 2651.577 kb on + strand, within Pf1N1B4_2333at 2651.577 kb on + strand, within Pf1N1B4_2333at 2651.577 kb on + strand, within Pf1N1B4_2333at 2651.577 kb on + strand, within Pf1N1B4_2333at 2651.578 kb on - strand, within Pf1N1B4_2333at 2651.578 kb on - strand, within Pf1N1B4_2333at 2651.578 kb on - strand, within Pf1N1B4_2333at 2651.578 kb on - strand, within Pf1N1B4_2333at 2651.578 kb on - strand, within Pf1N1B4_2333at 2651.578 kb on - strand, within Pf1N1B4_2333at 2651.578 kb on - strandat 2651.578 kb on - strand, within Pf1N1B4_2333at 2651.578 kb on - strand, within Pf1N1B4_2333at 2651.578 kb on - strand, within Pf1N1B4_2333at 2651.578 kb on - strand, within Pf1N1B4_2333at 2651.578 kb on - strand, within Pf1N1B4_2333at 2651.578 kb on - strand, within Pf1N1B4_2333at 2651.622 kb on + strand, within Pf1N1B4_2333at 2651.622 kb on + strand, within Pf1N1B4_2333at 2651.622 kb on + strand, within Pf1N1B4_2333at 2651.623 kb on - strand, within Pf1N1B4_2333at 2651.623 kb on - strand, within Pf1N1B4_2333at 2651.623 kb on - strand, within Pf1N1B4_2333at 2651.623 kb on - strand, within Pf1N1B4_2333at 2651.623 kb on - strand, within Pf1N1B4_2333at 2651.623 kb on - strand, within Pf1N1B4_2333at 2651.623 kb on - strand, within Pf1N1B4_2333at 2651.623 kb on - strand, within Pf1N1B4_2333at 2651.623 kb on - strand, within Pf1N1B4_2333at 2651.623 kb on - strand, within Pf1N1B4_2333at 2651.623 kb on - strand, within Pf1N1B4_2333at 2651.847 kb on + strandat 2651.847 kb on + strandat 2651.847 kb on + strandat 2651.847 kb on + strandat 2652.117 kb on + strand, within Pf1N1B4_2334at 2652.118 kb on - strand, within Pf1N1B4_2334at 2652.235 kb on + strand, within Pf1N1B4_2334at 2652.279 kb on + strandat 2652.502 kb on + strand, within Pf1N1B4_2334at 2652.822 kb on + strand, within Pf1N1B4_2334at 2652.870 kb on + strand, within Pf1N1B4_2334at 2652.870 kb on + strand, within Pf1N1B4_2334at 2652.871 kb on - strand, within Pf1N1B4_2334at 2652.921 kb on + strand, within Pf1N1B4_2334at 2652.922 kb on - strand, within Pf1N1B4_2334at 2652.957 kb on + strandat 2652.957 kb on + strandat 2652.957 kb on + strandat 2652.958 kb on - strandat 2652.958 kb on - strandat 2652.958 kb on - strandat 2653.125 kb on + strand, within Pf1N1B4_2335at 2653.125 kb on + strand, within Pf1N1B4_2335at 2653.126 kb on - strand, within Pf1N1B4_2335at 2653.126 kb on - strand, within Pf1N1B4_2335at 2653.126 kb on - strand, within Pf1N1B4_2335at 2653.126 kb on - strand, within Pf1N1B4_2335at 2653.389 kb on - strand, within Pf1N1B4_2335at 2653.389 kb on - strand, within Pf1N1B4_2335at 2653.389 kb on - strand, within Pf1N1B4_2335at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strandat 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.757 kb on - strand, within Pf1N1B4_2336at 2653.757 kb on - strand, within Pf1N1B4_2336at 2653.759 kb on + strand, within Pf1N1B4_2336at 2653.856 kb on + strandat 2653.857 kb on - strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.964 kb on - strand, within Pf1N1B4_2336at 2653.964 kb on - strand, within Pf1N1B4_2336at 2654.048 kb on + strandat 2654.049 kb on - strandat 2654.049 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
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2,651,029 - Pf1N1B4_2332 0.49 -1.5
2,651,235 + Pf1N1B4_2332 0.78 +1.5
2,651,235 + Pf1N1B4_2332 0.78 +1.2
2,651,235 + Pf1N1B4_2332 0.78 -0.4
2,651,394 + -0.1
2,651,394 + -2.1
2,651,504 + -0.1
2,651,504 + +0.9
2,651,505 - -2.8
2,651,505 - -2.6
2,651,577 + Pf1N1B4_2333 0.20 +0.9
2,651,577 + Pf1N1B4_2333 0.20 +0.8
2,651,577 + Pf1N1B4_2333 0.20 -2.6
2,651,577 + Pf1N1B4_2333 0.20 -0.3
2,651,577 + Pf1N1B4_2333 0.20 +2.3
2,651,577 + Pf1N1B4_2333 0.20 +0.9
2,651,577 + Pf1N1B4_2333 0.20 -0.1
2,651,578 - Pf1N1B4_2333 0.20 +0.9
2,651,578 - Pf1N1B4_2333 0.20 +0.4
2,651,578 - Pf1N1B4_2333 0.20 -0.5
2,651,578 - Pf1N1B4_2333 0.20 -2.8
2,651,578 - Pf1N1B4_2333 0.20 -1.6
2,651,578 - Pf1N1B4_2333 0.20 -0.5
2,651,578 - -2.0
2,651,578 - Pf1N1B4_2333 0.20 -0.3
2,651,578 - Pf1N1B4_2333 0.20 -2.0
2,651,578 - Pf1N1B4_2333 0.20 +0.9
2,651,578 - Pf1N1B4_2333 0.20 -0.4
2,651,578 - Pf1N1B4_2333 0.20 -0.1
2,651,578 - Pf1N1B4_2333 0.20 -1.2
2,651,622 + Pf1N1B4_2333 0.34 -2.3
2,651,622 + Pf1N1B4_2333 0.34 -1.1
2,651,622 + Pf1N1B4_2333 0.34 -1.2
2,651,623 - Pf1N1B4_2333 0.34 -0.1
2,651,623 - Pf1N1B4_2333 0.34 +0.3
2,651,623 - Pf1N1B4_2333 0.34 -0.3
2,651,623 - Pf1N1B4_2333 0.34 -1.0
2,651,623 - Pf1N1B4_2333 0.34 -2.6
2,651,623 - Pf1N1B4_2333 0.34 +0.3
2,651,623 - Pf1N1B4_2333 0.34 -0.7
2,651,623 - Pf1N1B4_2333 0.34 +0.9
2,651,623 - Pf1N1B4_2333 0.34 -3.8
2,651,623 - Pf1N1B4_2333 0.34 -1.7
2,651,623 - Pf1N1B4_2333 0.34 -0.8
2,651,847 + -1.1
2,651,847 + -0.3
2,651,847 + -1.8
2,651,847 + +1.0
2,652,117 + Pf1N1B4_2334 0.22 -2.5
2,652,118 - Pf1N1B4_2334 0.23 +0.3
2,652,235 + Pf1N1B4_2334 0.32 +0.9
2,652,279 + -2.0
2,652,502 + Pf1N1B4_2334 0.54 -1.7
2,652,822 + Pf1N1B4_2334 0.81 +0.9
2,652,870 + Pf1N1B4_2334 0.85 -1.3
2,652,870 + Pf1N1B4_2334 0.85 -0.7
2,652,871 - Pf1N1B4_2334 0.85 -2.0
2,652,921 + Pf1N1B4_2334 0.89 +0.9
2,652,922 - Pf1N1B4_2334 0.89 -2.3
2,652,957 + +2.1
2,652,957 + +0.9
2,652,957 + -1.5
2,652,958 - +0.9
2,652,958 - -1.0
2,652,958 - +0.9
2,653,125 + Pf1N1B4_2335 0.13 -2.0
2,653,125 + Pf1N1B4_2335 0.13 -2.8
2,653,126 - Pf1N1B4_2335 0.13 +0.9
2,653,126 - Pf1N1B4_2335 0.13 +0.9
2,653,126 - Pf1N1B4_2335 0.13 -2.3
2,653,126 - Pf1N1B4_2335 0.13 +0.9
2,653,389 - Pf1N1B4_2335 0.57 -0.9
2,653,389 - Pf1N1B4_2335 0.57 -0.7
2,653,389 - Pf1N1B4_2335 0.57 -1.5
2,653,756 + Pf1N1B4_2336 0.26 -3.2
2,653,756 + Pf1N1B4_2336 0.26 -0.1
2,653,756 + Pf1N1B4_2336 0.26 +0.9
2,653,756 + +0.9
2,653,756 + Pf1N1B4_2336 0.26 -0.6
2,653,756 + Pf1N1B4_2336 0.26 +0.1
2,653,756 + Pf1N1B4_2336 0.26 +0.9
2,653,757 - Pf1N1B4_2336 0.27 +0.9
2,653,757 - Pf1N1B4_2336 0.27 -0.3
2,653,759 + Pf1N1B4_2336 0.27 +0.9
2,653,856 + +0.9
2,653,857 - Pf1N1B4_2336 0.50 +0.9
2,653,963 + Pf1N1B4_2336 0.74 +0.9
2,653,963 + Pf1N1B4_2336 0.74 -0.6
2,653,963 + Pf1N1B4_2336 0.74 -2.3
2,653,963 + Pf1N1B4_2336 0.74 +0.9
2,653,963 + Pf1N1B4_2336 0.74 -0.0
2,653,963 + Pf1N1B4_2336 0.74 -0.7
2,653,964 - Pf1N1B4_2336 0.74 +0.3
2,653,964 - Pf1N1B4_2336 0.74 -2.5
2,654,048 + +0.9
2,654,049 - +0.9
2,654,049 - -1.6

Or see this region's nucleotide sequence