Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2139 and Pf1N1B4_2140 are separated by 179 nucleotides Pf1N1B4_2140 and Pf1N1B4_2141 are separated by 143 nucleotides
Pf1N1B4_2139 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 2,449,976 to 2,451,394
_2139
Pf1N1B4_2140 - FIG00957579: hypothetical protein, at 2,451,574 to 2,451,819
_2140
Pf1N1B4_2141 - Gamma-glutamyl-putrescine oxidase (EC1.4.3.-), at 2,451,963 to 2,453,276
_2141
Position (kb)
2451
2452 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2450.628 kb on + strand, within Pf1N1B4_2139 at 2450.628 kb on + strand, within Pf1N1B4_2139 at 2450.628 kb on + strand, within Pf1N1B4_2139 at 2450.628 kb on + strand, within Pf1N1B4_2139 at 2450.628 kb on + strand, within Pf1N1B4_2139 at 2450.628 kb on + strand, within Pf1N1B4_2139 at 2450.629 kb on - strand, within Pf1N1B4_2139 at 2450.629 kb on - strand, within Pf1N1B4_2139 at 2450.629 kb on - strand, within Pf1N1B4_2139 at 2450.629 kb on - strand, within Pf1N1B4_2139 at 2450.663 kb on + strand, within Pf1N1B4_2139 at 2450.663 kb on + strand, within Pf1N1B4_2139 at 2450.663 kb on + strand, within Pf1N1B4_2139 at 2450.663 kb on + strand, within Pf1N1B4_2139 at 2450.663 kb on + strand, within Pf1N1B4_2139 at 2450.663 kb on + strand, within Pf1N1B4_2139 at 2450.664 kb on - strand, within Pf1N1B4_2139 at 2450.664 kb on - strand at 2450.664 kb on - strand, within Pf1N1B4_2139 at 2450.664 kb on - strand, within Pf1N1B4_2139 at 2450.664 kb on - strand, within Pf1N1B4_2139 at 2450.664 kb on - strand, within Pf1N1B4_2139 at 2450.958 kb on + strand, within Pf1N1B4_2139 at 2450.958 kb on + strand, within Pf1N1B4_2139 at 2450.958 kb on + strand, within Pf1N1B4_2139 at 2450.958 kb on + strand, within Pf1N1B4_2139 at 2450.958 kb on + strand, within Pf1N1B4_2139 at 2450.958 kb on + strand, within Pf1N1B4_2139 at 2450.958 kb on + strand, within Pf1N1B4_2139 at 2450.958 kb on + strand, within Pf1N1B4_2139 at 2450.958 kb on + strand, within Pf1N1B4_2139 at 2450.958 kb on + strand, within Pf1N1B4_2139 at 2450.958 kb on + strand, within Pf1N1B4_2139 at 2450.959 kb on - strand, within Pf1N1B4_2139 at 2450.959 kb on - strand, within Pf1N1B4_2139 at 2450.959 kb on - strand at 2450.959 kb on - strand, within Pf1N1B4_2139 at 2450.959 kb on - strand, within Pf1N1B4_2139 at 2450.959 kb on - strand, within Pf1N1B4_2139 at 2450.959 kb on - strand, within Pf1N1B4_2139 at 2450.970 kb on + strand, within Pf1N1B4_2139 at 2450.970 kb on + strand, within Pf1N1B4_2139 at 2450.971 kb on - strand, within Pf1N1B4_2139 at 2450.971 kb on - strand, within Pf1N1B4_2139 at 2450.971 kb on - strand, within Pf1N1B4_2139 at 2450.973 kb on + strand, within Pf1N1B4_2139 at 2450.973 kb on + strand, within Pf1N1B4_2139 at 2450.973 kb on + strand, within Pf1N1B4_2139 at 2450.974 kb on - strand, within Pf1N1B4_2139 at 2450.974 kb on - strand, within Pf1N1B4_2139 at 2451.133 kb on + strand, within Pf1N1B4_2139 at 2451.133 kb on + strand, within Pf1N1B4_2139 at 2451.134 kb on - strand, within Pf1N1B4_2139 at 2451.164 kb on + strand, within Pf1N1B4_2139 at 2451.164 kb on + strand, within Pf1N1B4_2139 at 2451.164 kb on + strand, within Pf1N1B4_2139 at 2451.165 kb on - strand, within Pf1N1B4_2139 at 2451.165 kb on - strand, within Pf1N1B4_2139 at 2451.165 kb on - strand, within Pf1N1B4_2139 at 2451.165 kb on - strand, within Pf1N1B4_2139 at 2451.165 kb on - strand, within Pf1N1B4_2139 at 2451.165 kb on - strand, within Pf1N1B4_2139 at 2451.255 kb on + strand at 2451.336 kb on + strand at 2451.337 kb on - strand at 2451.384 kb on + strand at 2451.384 kb on + strand at 2451.384 kb on + strand at 2451.384 kb on + strand at 2451.384 kb on + strand at 2451.384 kb on + strand at 2451.385 kb on - strand at 2451.385 kb on - strand at 2451.385 kb on - strand at 2451.431 kb on - strand at 2451.447 kb on + strand at 2451.447 kb on + strand at 2451.447 kb on + strand at 2451.447 kb on + strand at 2451.448 kb on - strand at 2451.448 kb on - strand at 2451.487 kb on + strand at 2451.488 kb on - strand at 2451.488 kb on - strand at 2451.488 kb on - strand at 2451.490 kb on + strand at 2451.556 kb on + strand at 2451.556 kb on + strand at 2451.556 kb on + strand at 2451.557 kb on - strand at 2451.605 kb on + strand, within Pf1N1B4_2140 at 2451.605 kb on + strand, within Pf1N1B4_2140 at 2451.606 kb on - strand, within Pf1N1B4_2140 at 2451.606 kb on - strand, within Pf1N1B4_2140 at 2451.798 kb on + strand at 2451.799 kb on - strand at 2451.799 kb on - strand at 2451.799 kb on - strand at 2451.824 kb on + strand at 2451.824 kb on + strand at 2451.824 kb on + strand at 2451.824 kb on + strand at 2451.824 kb on + strand at 2451.824 kb on + strand at 2451.825 kb on - strand at 2451.825 kb on - strand at 2451.825 kb on - strand at 2451.825 kb on - strand at 2451.825 kb on - strand at 2451.825 kb on - strand at 2451.825 kb on - strand at 2452.153 kb on + strand, within Pf1N1B4_2141 at 2452.165 kb on + strand at 2452.165 kb on + strand, within Pf1N1B4_2141 at 2452.165 kb on + strand, within Pf1N1B4_2141 at 2452.166 kb on - strand, within Pf1N1B4_2141 at 2452.168 kb on + strand, within Pf1N1B4_2141 at 2452.168 kb on + strand at 2452.168 kb on + strand, within Pf1N1B4_2141 at 2452.168 kb on + strand, within Pf1N1B4_2141 at 2452.168 kb on + strand, within Pf1N1B4_2141 at 2452.169 kb on - strand, within Pf1N1B4_2141 at 2452.169 kb on - strand, within Pf1N1B4_2141 at 2452.169 kb on - strand, within Pf1N1B4_2141 at 2452.169 kb on - strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.170 kb on + strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.171 kb on - strand, within Pf1N1B4_2141 at 2452.246 kb on + strand, within Pf1N1B4_2141 at 2452.303 kb on + strand, within Pf1N1B4_2141 at 2452.303 kb on + strand, within Pf1N1B4_2141 at 2452.303 kb on + strand, within Pf1N1B4_2141 at 2452.303 kb on + strand, within Pf1N1B4_2141 at 2452.304 kb on - strand, within Pf1N1B4_2141 at 2452.304 kb on - strand, within Pf1N1B4_2141 at 2452.304 kb on - strand, within Pf1N1B4_2141 at 2452.337 kb on - strand, within Pf1N1B4_2141 at 2452.384 kb on + strand, within Pf1N1B4_2141 at 2452.384 kb on + strand, within Pf1N1B4_2141 at 2452.387 kb on + strand, within Pf1N1B4_2141 at 2452.387 kb on + strand, within Pf1N1B4_2141 at 2452.388 kb on - strand, within Pf1N1B4_2141 at 2452.388 kb on - strand, within Pf1N1B4_2141 at 2452.388 kb on - strand, within Pf1N1B4_2141 at 2452.388 kb on - strand, within Pf1N1B4_2141 at 2452.388 kb on - strand, within Pf1N1B4_2141 at 2452.388 kb on - strand at 2452.388 kb on - strand at 2452.480 kb on + strand at 2452.480 kb on + strand, within Pf1N1B4_2141 at 2452.480 kb on + strand, within Pf1N1B4_2141 at 2452.480 kb on + strand, within Pf1N1B4_2141 at 2452.480 kb on + strand, within Pf1N1B4_2141 at 2452.480 kb on + strand, within Pf1N1B4_2141 at 2452.480 kb on + strand, within Pf1N1B4_2141 at 2452.481 kb on - strand, within Pf1N1B4_2141 at 2452.481 kb on - strand, within Pf1N1B4_2141 at 2452.481 kb on - strand, within Pf1N1B4_2141 at 2452.535 kb on - strand, within Pf1N1B4_2141 at 2452.535 kb on - strand, within Pf1N1B4_2141 at 2452.535 kb on - strand, within Pf1N1B4_2141 at 2452.535 kb on - strand, within Pf1N1B4_2141 at 2452.739 kb on - strand, within Pf1N1B4_2141
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 2,450,628 + Pf1N1B4_2139 0.46 +0.3 2,450,628 + Pf1N1B4_2139 0.46 -0.6 2,450,628 + Pf1N1B4_2139 0.46 +0.9 2,450,628 + Pf1N1B4_2139 0.46 -2.6 2,450,628 + Pf1N1B4_2139 0.46 +0.9 2,450,628 + Pf1N1B4_2139 0.46 -2.1 2,450,629 - Pf1N1B4_2139 0.46 -0.4 2,450,629 - Pf1N1B4_2139 0.46 +0.0 2,450,629 - Pf1N1B4_2139 0.46 -0.7 2,450,629 - Pf1N1B4_2139 0.46 -1.7 2,450,663 + Pf1N1B4_2139 0.48 +0.5 2,450,663 + Pf1N1B4_2139 0.48 -1.3 2,450,663 + Pf1N1B4_2139 0.48 +0.9 2,450,663 + Pf1N1B4_2139 0.48 -1.9 2,450,663 + Pf1N1B4_2139 0.48 +0.9 2,450,663 + Pf1N1B4_2139 0.48 +0.1 2,450,664 - Pf1N1B4_2139 0.48 -2.5 2,450,664 - -1.2 2,450,664 - Pf1N1B4_2139 0.48 -0.3 2,450,664 - Pf1N1B4_2139 0.48 +0.3 2,450,664 - Pf1N1B4_2139 0.48 +0.2 2,450,664 - Pf1N1B4_2139 0.48 -0.2 2,450,958 + Pf1N1B4_2139 0.69 +0.6 2,450,958 + Pf1N1B4_2139 0.69 -0.7 2,450,958 + Pf1N1B4_2139 0.69 -1.2 2,450,958 + Pf1N1B4_2139 0.69 +0.1 2,450,958 + Pf1N1B4_2139 0.69 -0.1 2,450,958 + Pf1N1B4_2139 0.69 -1.3 2,450,958 + Pf1N1B4_2139 0.69 -2.3 2,450,958 + Pf1N1B4_2139 0.69 -1.4 2,450,958 + Pf1N1B4_2139 0.69 +0.9 2,450,958 + Pf1N1B4_2139 0.69 -0.4 2,450,958 + Pf1N1B4_2139 0.69 +0.9 2,450,959 - Pf1N1B4_2139 0.69 +0.5 2,450,959 - Pf1N1B4_2139 0.69 +0.1 2,450,959 - +0.9 2,450,959 - Pf1N1B4_2139 0.69 +0.9 2,450,959 - Pf1N1B4_2139 0.69 -1.5 2,450,959 - Pf1N1B4_2139 0.69 +1.7 2,450,959 - Pf1N1B4_2139 0.69 +0.9 2,450,970 + Pf1N1B4_2139 0.70 +0.9 2,450,970 + Pf1N1B4_2139 0.70 -2.8 2,450,971 - Pf1N1B4_2139 0.70 -1.1 2,450,971 - Pf1N1B4_2139 0.70 -3.2 2,450,971 - Pf1N1B4_2139 0.70 -0.3 2,450,973 + Pf1N1B4_2139 0.70 +0.9 2,450,973 + Pf1N1B4_2139 0.70 +0.9 2,450,973 + Pf1N1B4_2139 0.70 -2.6 2,450,974 - Pf1N1B4_2139 0.70 +0.9 2,450,974 - Pf1N1B4_2139 0.70 -1.5 2,451,133 + Pf1N1B4_2139 0.82 +0.1 2,451,133 + Pf1N1B4_2139 0.82 +1.1 2,451,134 - Pf1N1B4_2139 0.82 +0.2 2,451,164 + Pf1N1B4_2139 0.84 -0.5 2,451,164 + Pf1N1B4_2139 0.84 +0.9 2,451,164 + Pf1N1B4_2139 0.84 -2.8 2,451,165 - Pf1N1B4_2139 0.84 -1.7 2,451,165 - Pf1N1B4_2139 0.84 +0.9 2,451,165 - Pf1N1B4_2139 0.84 -2.4 2,451,165 - Pf1N1B4_2139 0.84 -4.0 2,451,165 - Pf1N1B4_2139 0.84 -2.1 2,451,165 - Pf1N1B4_2139 0.84 -0.1 2,451,255 + -2.3 2,451,336 + +0.2 2,451,337 - -1.7 2,451,384 + -0.4 2,451,384 + +0.2 2,451,384 + +0.9 2,451,384 + +0.9 2,451,384 + -2.5 2,451,384 + -1.7 2,451,385 - -0.6 2,451,385 - +2.0 2,451,385 - -0.4 2,451,431 - -1.3 2,451,447 + -1.5 2,451,447 + -0.6 2,451,447 + -1.9 2,451,447 + +0.1 2,451,448 - +0.7 2,451,448 - -0.1 2,451,487 + +0.9 2,451,488 - -0.5 2,451,488 - -0.3 2,451,488 - +0.9 2,451,490 + +0.1 2,451,556 + -0.1 2,451,556 + -2.8 2,451,556 + +2.4 2,451,557 - +0.9 2,451,605 + Pf1N1B4_2140 0.13 -0.0 2,451,605 + Pf1N1B4_2140 0.13 +0.1 2,451,606 - Pf1N1B4_2140 0.13 +0.4 2,451,606 - Pf1N1B4_2140 0.13 +0.1 2,451,798 + +0.7 2,451,799 - -0.5 2,451,799 - -0.4 2,451,799 - +1.3 2,451,824 + -1.0 2,451,824 + -1.7 2,451,824 + -0.1 2,451,824 + +0.9 2,451,824 + -1.7 2,451,824 + -0.7 2,451,825 - +0.9 2,451,825 - +0.9 2,451,825 - -0.1 2,451,825 - +0.9 2,451,825 - -3.4 2,451,825 - -1.5 2,451,825 - -1.1 2,452,153 + Pf1N1B4_2141 0.14 +0.9 2,452,165 + +0.9 2,452,165 + Pf1N1B4_2141 0.15 -3.0 2,452,165 + Pf1N1B4_2141 0.15 +0.9 2,452,166 - Pf1N1B4_2141 0.15 -0.4 2,452,168 + Pf1N1B4_2141 0.16 +0.9 2,452,168 + -2.1 2,452,168 + Pf1N1B4_2141 0.16 -0.3 2,452,168 + Pf1N1B4_2141 0.16 -2.5 2,452,168 + Pf1N1B4_2141 0.16 -1.4 2,452,169 - Pf1N1B4_2141 0.16 +1.0 2,452,169 - Pf1N1B4_2141 0.16 -0.1 2,452,169 - Pf1N1B4_2141 0.16 +0.9 2,452,169 - Pf1N1B4_2141 0.16 -1.7 2,452,170 + Pf1N1B4_2141 0.16 +0.9 2,452,170 + Pf1N1B4_2141 0.16 +0.9 2,452,170 + Pf1N1B4_2141 0.16 -1.6 2,452,170 + Pf1N1B4_2141 0.16 +0.9 2,452,170 + Pf1N1B4_2141 0.16 +0.5 2,452,170 + Pf1N1B4_2141 0.16 +0.9 2,452,170 + Pf1N1B4_2141 0.16 +0.9 2,452,170 + Pf1N1B4_2141 0.16 +0.9 2,452,170 + Pf1N1B4_2141 0.16 -0.5 2,452,170 + Pf1N1B4_2141 0.16 -2.3 2,452,170 + Pf1N1B4_2141 0.16 +0.9 2,452,170 + Pf1N1B4_2141 0.16 -1.4 2,452,170 + Pf1N1B4_2141 0.16 -0.7 2,452,170 + Pf1N1B4_2141 0.16 -3.4 2,452,170 + Pf1N1B4_2141 0.16 -0.7 2,452,170 + Pf1N1B4_2141 0.16 +0.9 2,452,171 - Pf1N1B4_2141 0.16 -2.5 2,452,171 - Pf1N1B4_2141 0.16 +0.9 2,452,171 - Pf1N1B4_2141 0.16 +0.2 2,452,171 - Pf1N1B4_2141 0.16 +0.9 2,452,171 - Pf1N1B4_2141 0.16 +0.9 2,452,171 - Pf1N1B4_2141 0.16 -0.7 2,452,171 - Pf1N1B4_2141 0.16 +0.9 2,452,171 - Pf1N1B4_2141 0.16 -1.1 2,452,171 - Pf1N1B4_2141 0.16 +0.9 2,452,171 - Pf1N1B4_2141 0.16 +0.9 2,452,171 - Pf1N1B4_2141 0.16 +0.9 2,452,171 - Pf1N1B4_2141 0.16 +0.9 2,452,171 - Pf1N1B4_2141 0.16 +0.9 2,452,171 - Pf1N1B4_2141 0.16 -0.4 2,452,171 - Pf1N1B4_2141 0.16 -0.5 2,452,171 - Pf1N1B4_2141 0.16 -0.4 2,452,171 - Pf1N1B4_2141 0.16 -1.0 2,452,171 - Pf1N1B4_2141 0.16 -1.7 2,452,171 - Pf1N1B4_2141 0.16 -1.6 2,452,171 - Pf1N1B4_2141 0.16 -0.7 2,452,246 + Pf1N1B4_2141 0.22 +0.9 2,452,303 + Pf1N1B4_2141 0.26 -2.3 2,452,303 + Pf1N1B4_2141 0.26 -1.1 2,452,303 + Pf1N1B4_2141 0.26 -3.0 2,452,303 + Pf1N1B4_2141 0.26 +0.9 2,452,304 - Pf1N1B4_2141 0.26 -1.1 2,452,304 - Pf1N1B4_2141 0.26 -2.7 2,452,304 - Pf1N1B4_2141 0.26 +0.9 2,452,337 - Pf1N1B4_2141 0.28 +2.0 2,452,384 + Pf1N1B4_2141 0.32 +0.9 2,452,384 + Pf1N1B4_2141 0.32 +1.2 2,452,387 + Pf1N1B4_2141 0.32 +1.1 2,452,387 + Pf1N1B4_2141 0.32 +0.0 2,452,388 - Pf1N1B4_2141 0.32 +0.9 2,452,388 - Pf1N1B4_2141 0.32 -2.3 2,452,388 - Pf1N1B4_2141 0.32 -0.5 2,452,388 - Pf1N1B4_2141 0.32 +0.9 2,452,388 - Pf1N1B4_2141 0.32 -3.3 2,452,388 - +0.9 2,452,388 - -1.9 2,452,480 + +0.9 2,452,480 + Pf1N1B4_2141 0.39 +0.9 2,452,480 + Pf1N1B4_2141 0.39 -0.1 2,452,480 + Pf1N1B4_2141 0.39 -0.3 2,452,480 + Pf1N1B4_2141 0.39 +0.9 2,452,480 + Pf1N1B4_2141 0.39 -3.2 2,452,480 + Pf1N1B4_2141 0.39 -1.6 2,452,481 - Pf1N1B4_2141 0.39 +0.9 2,452,481 - Pf1N1B4_2141 0.39 -2.1 2,452,481 - Pf1N1B4_2141 0.39 +0.9 2,452,535 - Pf1N1B4_2141 0.44 +0.1 2,452,535 - Pf1N1B4_2141 0.44 +0.9 2,452,535 - Pf1N1B4_2141 0.44 +0.9 2,452,535 - Pf1N1B4_2141 0.44 -0.7 2,452,739 - Pf1N1B4_2141 0.59 +0.9
Or see this region's nucleotide sequence