Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1703 and Pf1N1B4_1704 are separated by 446 nucleotides Pf1N1B4_1704 and Pf1N1B4_1705 overlap by 4 nucleotides Pf1N1B4_1705 and Pf1N1B4_1706 are separated by 108 nucleotides
Pf1N1B4_1703 - Methionine ABC transporter permease protein, at 1,949,728 to 1,950,372
_1703
Pf1N1B4_1704 - hypothetical protein, at 1,950,819 to 1,950,932
_1704
Pf1N1B4_1705 - SAM-dependent methyltransferases, at 1,950,929 to 1,951,558
_1705
Pf1N1B4_1706 - RND efflux transporter, at 1,951,667 to 1,954,720
_1706
Position (kb)
1950
1951
1952 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1949.977 kb on + strand, within Pf1N1B4_1703 at 1949.978 kb on - strand, within Pf1N1B4_1703 at 1950.175 kb on - strand, within Pf1N1B4_1703 at 1950.175 kb on - strand, within Pf1N1B4_1703 at 1950.281 kb on + strand, within Pf1N1B4_1703 at 1950.281 kb on + strand, within Pf1N1B4_1703 at 1950.363 kb on + strand at 1950.379 kb on + strand at 1950.379 kb on + strand at 1950.379 kb on + strand at 1950.380 kb on - strand at 1950.402 kb on + strand at 1950.402 kb on + strand at 1950.403 kb on - strand at 1950.541 kb on + strand at 1950.542 kb on - strand at 1950.542 kb on - strand at 1950.548 kb on + strand at 1950.562 kb on + strand at 1950.562 kb on + strand at 1950.586 kb on + strand at 1950.586 kb on + strand at 1950.586 kb on + strand at 1950.587 kb on - strand at 1950.587 kb on - strand at 1950.607 kb on + strand at 1950.687 kb on + strand at 1950.688 kb on - strand at 1950.892 kb on + strand, within Pf1N1B4_1704 at 1950.893 kb on - strand, within Pf1N1B4_1704 at 1950.893 kb on - strand, within Pf1N1B4_1704 at 1950.893 kb on - strand, within Pf1N1B4_1704 at 1950.893 kb on - strand, within Pf1N1B4_1704 at 1950.894 kb on + strand, within Pf1N1B4_1704 at 1950.895 kb on - strand, within Pf1N1B4_1704 at 1950.912 kb on + strand, within Pf1N1B4_1704 at 1950.912 kb on + strand, within Pf1N1B4_1704 at 1950.912 kb on + strand, within Pf1N1B4_1704 at 1950.913 kb on - strand, within Pf1N1B4_1704 at 1950.983 kb on + strand at 1950.983 kb on + strand at 1950.983 kb on + strand at 1950.983 kb on + strand at 1950.983 kb on + strand at 1950.984 kb on - strand at 1950.984 kb on - strand at 1950.984 kb on - strand at 1950.984 kb on - strand at 1950.984 kb on - strand at 1950.984 kb on - strand at 1951.063 kb on + strand, within Pf1N1B4_1705 at 1951.424 kb on + strand, within Pf1N1B4_1705 at 1951.425 kb on - strand, within Pf1N1B4_1705 at 1951.425 kb on - strand, within Pf1N1B4_1705 at 1951.425 kb on - strand, within Pf1N1B4_1705 at 1951.427 kb on + strand, within Pf1N1B4_1705 at 1951.487 kb on + strand, within Pf1N1B4_1705 at 1951.668 kb on + strand at 1951.702 kb on - strand at 1951.702 kb on - strand at 1951.713 kb on + strand at 1952.183 kb on + strand, within Pf1N1B4_1706 at 1952.252 kb on + strand, within Pf1N1B4_1706 at 1952.253 kb on - strand, within Pf1N1B4_1706 at 1952.359 kb on - strand, within Pf1N1B4_1706 at 1952.484 kb on + strand, within Pf1N1B4_1706 at 1952.484 kb on + strand, within Pf1N1B4_1706 at 1952.485 kb on - strand, within Pf1N1B4_1706 at 1952.485 kb on - strand, within Pf1N1B4_1706 at 1952.485 kb on - strand, within Pf1N1B4_1706 at 1952.488 kb on + strand at 1952.488 kb on + strand, within Pf1N1B4_1706 at 1952.488 kb on + strand, within Pf1N1B4_1706 at 1952.488 kb on + strand, within Pf1N1B4_1706 at 1952.488 kb on + strand, within Pf1N1B4_1706 at 1952.488 kb on + strand, within Pf1N1B4_1706 at 1952.488 kb on + strand, within Pf1N1B4_1706 at 1952.488 kb on + strand, within Pf1N1B4_1706 at 1952.489 kb on - strand, within Pf1N1B4_1706 at 1952.489 kb on - strand, within Pf1N1B4_1706 at 1952.489 kb on - strand, within Pf1N1B4_1706 at 1952.489 kb on - strand, within Pf1N1B4_1706 at 1952.489 kb on - strand, within Pf1N1B4_1706
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 1,949,977 + Pf1N1B4_1703 0.39 -1.5 1,949,978 - Pf1N1B4_1703 0.39 -1.7 1,950,175 - Pf1N1B4_1703 0.69 +1.3 1,950,175 - Pf1N1B4_1703 0.69 -1.7 1,950,281 + Pf1N1B4_1703 0.86 +2.1 1,950,281 + Pf1N1B4_1703 0.86 -0.1 1,950,363 + -3.7 1,950,379 + -0.7 1,950,379 + -3.2 1,950,379 + -2.5 1,950,380 - -3.4 1,950,402 + -1.1 1,950,402 + -1.5 1,950,403 - +0.9 1,950,541 + -1.5 1,950,542 - -1.3 1,950,542 - +0.2 1,950,548 + +0.1 1,950,562 + +0.9 1,950,562 + +0.6 1,950,586 + +0.9 1,950,586 + +0.9 1,950,586 + +0.9 1,950,587 - -3.3 1,950,587 - +0.9 1,950,607 + +0.9 1,950,687 + -0.5 1,950,688 - -0.1 1,950,892 + Pf1N1B4_1704 0.64 +0.2 1,950,893 - Pf1N1B4_1704 0.65 -0.5 1,950,893 - Pf1N1B4_1704 0.65 +0.9 1,950,893 - Pf1N1B4_1704 0.65 -0.1 1,950,893 - Pf1N1B4_1704 0.65 +0.9 1,950,894 + Pf1N1B4_1704 0.66 +0.9 1,950,895 - Pf1N1B4_1704 0.67 +0.6 1,950,912 + Pf1N1B4_1704 0.82 -0.9 1,950,912 + Pf1N1B4_1704 0.82 +0.9 1,950,912 + Pf1N1B4_1704 0.82 +0.3 1,950,913 - Pf1N1B4_1704 0.82 +0.9 1,950,983 + -3.3 1,950,983 + -1.7 1,950,983 + +0.9 1,950,983 + +0.2 1,950,983 + -1.1 1,950,984 - +0.9 1,950,984 - -1.7 1,950,984 - -0.1 1,950,984 - +1.2 1,950,984 - +0.9 1,950,984 - +0.9 1,951,063 + Pf1N1B4_1705 0.21 -3.8 1,951,424 + Pf1N1B4_1705 0.79 -1.8 1,951,425 - Pf1N1B4_1705 0.79 -2.5 1,951,425 - Pf1N1B4_1705 0.79 -2.5 1,951,425 - Pf1N1B4_1705 0.79 -0.7 1,951,427 + Pf1N1B4_1705 0.79 +0.9 1,951,487 + Pf1N1B4_1705 0.89 +0.9 1,951,668 + +0.3 1,951,702 - -0.7 1,951,702 - +0.9 1,951,713 + -1.7 1,952,183 + Pf1N1B4_1706 0.17 +0.9 1,952,252 + Pf1N1B4_1706 0.19 +0.9 1,952,253 - Pf1N1B4_1706 0.19 -0.2 1,952,359 - Pf1N1B4_1706 0.23 +0.9 1,952,484 + Pf1N1B4_1706 0.27 -1.1 1,952,484 + Pf1N1B4_1706 0.27 +0.9 1,952,485 - Pf1N1B4_1706 0.27 -0.1 1,952,485 - Pf1N1B4_1706 0.27 -2.5 1,952,485 - Pf1N1B4_1706 0.27 -0.7 1,952,488 + -2.6 1,952,488 + Pf1N1B4_1706 0.27 +0.9 1,952,488 + Pf1N1B4_1706 0.27 +0.2 1,952,488 + Pf1N1B4_1706 0.27 -2.6 1,952,488 + Pf1N1B4_1706 0.27 -1.1 1,952,488 + Pf1N1B4_1706 0.27 +0.9 1,952,488 + Pf1N1B4_1706 0.27 -0.7 1,952,488 + Pf1N1B4_1706 0.27 -0.1 1,952,489 - Pf1N1B4_1706 0.27 +0.9 1,952,489 - Pf1N1B4_1706 0.27 -0.9 1,952,489 - Pf1N1B4_1706 0.27 -1.7 1,952,489 - Pf1N1B4_1706 0.27 -1.6 1,952,489 - Pf1N1B4_1706 0.27 +0.9
Or see this region's nucleotide sequence