Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_163 and Pf1N1B4_164 overlap by 4 nucleotides Pf1N1B4_164 and Pf1N1B4_165 are separated by 124 nucleotides Pf1N1B4_165 and Pf1N1B4_166 are separated by 139 nucleotides
Pf1N1B4_163 - ADP-ribosylglycohydrolase, at 204,369 to 205,313
_163
Pf1N1B4_164 - ADP-ribosylglycohydrolase family protein, at 205,310 to 205,708
_164
Pf1N1B4_165 - FIG00638667: hypothetical protein, at 205,833 to 206,210
_165
Pf1N1B4_166 - Quinohemoprotein amine dehydrogenase beta subunit (EC 1.4.99.-), at 206,350 to 207,468
_166
Position (kb) 205
206 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 204.355 kb on + strand at 204.355 kb on + strand at 204.356 kb on - strand at 204.672 kb on - strand, within Pf1N1B4_163 at 204.672 kb on - strand, within Pf1N1B4_163 at 204.672 kb on - strand at 204.672 kb on - strand, within Pf1N1B4_163 at 204.672 kb on - strand at 204.782 kb on - strand, within Pf1N1B4_163 at 204.789 kb on + strand, within Pf1N1B4_163 at 204.792 kb on + strand, within Pf1N1B4_163 at 204.792 kb on + strand, within Pf1N1B4_163 at 204.792 kb on + strand, within Pf1N1B4_163 at 204.793 kb on - strand, within Pf1N1B4_163 at 205.176 kb on + strand, within Pf1N1B4_163 at 205.315 kb on + strand at 205.353 kb on - strand, within Pf1N1B4_164 at 205.497 kb on - strand, within Pf1N1B4_164 at 205.497 kb on - strand, within Pf1N1B4_164 at 205.581 kb on + strand, within Pf1N1B4_164 at 205.582 kb on - strand, within Pf1N1B4_164 at 205.582 kb on - strand, within Pf1N1B4_164 at 205.670 kb on + strand at 205.670 kb on + strand at 205.706 kb on + strand at 205.706 kb on + strand at 205.706 kb on + strand at 205.706 kb on + strand at 205.707 kb on - strand at 205.707 kb on - strand at 206.094 kb on + strand, within Pf1N1B4_165 at 206.107 kb on - strand, within Pf1N1B4_165 at 206.160 kb on + strand at 206.160 kb on + strand, within Pf1N1B4_165 at 206.160 kb on + strand, within Pf1N1B4_165 at 206.160 kb on + strand, within Pf1N1B4_165 at 206.160 kb on + strand, within Pf1N1B4_165 at 206.160 kb on + strand at 206.160 kb on + strand, within Pf1N1B4_165 at 206.161 kb on - strand at 206.295 kb on + strand at 206.296 kb on - strand at 206.471 kb on + strand, within Pf1N1B4_166 at 206.507 kb on + strand, within Pf1N1B4_166 at 206.510 kb on + strand, within Pf1N1B4_166 at 206.510 kb on + strand at 206.510 kb on + strand, within Pf1N1B4_166 at 206.510 kb on + strand, within Pf1N1B4_166 at 206.510 kb on + strand at 206.511 kb on - strand, within Pf1N1B4_166 at 206.511 kb on - strand, within Pf1N1B4_166 at 206.561 kb on + strand, within Pf1N1B4_166 at 206.562 kb on - strand, within Pf1N1B4_166 at 206.630 kb on + strand, within Pf1N1B4_166 at 206.630 kb on + strand, within Pf1N1B4_166 at 206.631 kb on - strand, within Pf1N1B4_166
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 204,355 + -0.1 204,355 + -0.9 204,356 - +0.7 204,672 - Pf1N1B4_163 0.32 -0.3 204,672 - Pf1N1B4_163 0.32 -1.9 204,672 - -1.6 204,672 - Pf1N1B4_163 0.32 +0.7 204,672 - +0.7 204,782 - Pf1N1B4_163 0.44 +0.7 204,789 + Pf1N1B4_163 0.44 -2.5 204,792 + Pf1N1B4_163 0.45 -2.6 204,792 + Pf1N1B4_163 0.45 -3.1 204,792 + Pf1N1B4_163 0.45 +0.2 204,793 - Pf1N1B4_163 0.45 -2.2 205,176 + Pf1N1B4_163 0.85 -2.5 205,315 + -0.1 205,353 - Pf1N1B4_164 0.11 -1.4 205,497 - Pf1N1B4_164 0.47 +0.0 205,497 - Pf1N1B4_164 0.47 +1.0 205,581 + Pf1N1B4_164 0.68 +0.7 205,582 - Pf1N1B4_164 0.68 -0.3 205,582 - Pf1N1B4_164 0.68 -0.9 205,670 + +0.7 205,670 + +0.7 205,706 + -2.0 205,706 + -0.3 205,706 + +0.7 205,706 + -0.2 205,707 - -1.0 205,707 - +0.7 206,094 + Pf1N1B4_165 0.69 +2.0 206,107 - Pf1N1B4_165 0.72 +0.7 206,160 + +1.3 206,160 + Pf1N1B4_165 0.87 -2.1 206,160 + Pf1N1B4_165 0.87 +0.7 206,160 + Pf1N1B4_165 0.87 +0.7 206,160 + Pf1N1B4_165 0.87 +0.5 206,160 + -2.2 206,160 + Pf1N1B4_165 0.87 -0.1 206,161 - -0.9 206,295 + -1.5 206,296 - -1.3 206,471 + Pf1N1B4_166 0.11 -0.7 206,507 + Pf1N1B4_166 0.14 +0.9 206,510 + Pf1N1B4_166 0.14 +0.7 206,510 + +0.7 206,510 + Pf1N1B4_166 0.14 +0.3 206,510 + Pf1N1B4_166 0.14 +0.3 206,510 + -0.9 206,511 - Pf1N1B4_166 0.14 -0.4 206,511 - Pf1N1B4_166 0.14 +0.7 206,561 + Pf1N1B4_166 0.19 -0.3 206,562 - Pf1N1B4_166 0.19 +0.1 206,630 + Pf1N1B4_166 0.25 +0.4 206,630 + Pf1N1B4_166 0.25 -0.6 206,631 - Pf1N1B4_166 0.25 -2.1
Or see this region's nucleotide sequence