Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1354

Experiment: N2E2wt with N1B4m denitrifying repB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1353 and Pf1N1B4_1354 are separated by 67 nucleotidesPf1N1B4_1354 and Pf1N1B4_1355 are separated by 41 nucleotides Pf1N1B4_1353 - Urease alpha subunit (EC 3.5.1.5), at 1,547,911 to 1,549,611 _1353 Pf1N1B4_1354 - hypothetical protein, at 1,549,679 to 1,552,135 _1354 Pf1N1B4_1355 - hypothetical protein, at 1,552,177 to 1,554,810 _1355 Position (kb) 1549 1550 1551 1552 1553Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1548.718 kb on + strand, within Pf1N1B4_1353at 1548.719 kb on - strand, within Pf1N1B4_1353at 1548.719 kb on - strand, within Pf1N1B4_1353at 1548.785 kb on + strand, within Pf1N1B4_1353at 1548.786 kb on - strand, within Pf1N1B4_1353at 1548.786 kb on - strand, within Pf1N1B4_1353at 1548.786 kb on - strand, within Pf1N1B4_1353at 1549.114 kb on + strand, within Pf1N1B4_1353at 1549.115 kb on - strand, within Pf1N1B4_1353at 1549.115 kb on - strand, within Pf1N1B4_1353at 1549.126 kb on + strand, within Pf1N1B4_1353at 1549.126 kb on + strand, within Pf1N1B4_1353at 1549.126 kb on + strand, within Pf1N1B4_1353at 1549.126 kb on + strand, within Pf1N1B4_1353at 1549.233 kb on - strand, within Pf1N1B4_1353at 1549.495 kb on + strandat 1549.496 kb on - strandat 1549.496 kb on - strandat 1549.528 kb on + strandat 1549.528 kb on + strandat 1549.528 kb on + strandat 1549.528 kb on + strandat 1549.528 kb on + strandat 1549.529 kb on - strandat 1549.529 kb on - strandat 1549.556 kb on + strandat 1549.597 kb on + strandat 1549.597 kb on + strandat 1549.597 kb on + strandat 1549.598 kb on - strandat 1549.620 kb on + strandat 1549.620 kb on + strandat 1549.620 kb on + strandat 1549.621 kb on - strandat 1549.736 kb on - strandat 1549.739 kb on + strandat 1549.740 kb on - strandat 1549.740 kb on - strandat 1549.740 kb on - strandat 1549.740 kb on - strandat 1549.740 kb on - strandat 1549.740 kb on - strandat 1549.740 kb on - strandat 1549.740 kb on - strandat 1549.740 kb on - strandat 1549.791 kb on + strandat 1549.919 kb on + strandat 1549.919 kb on + strandat 1549.935 kb on - strand, within Pf1N1B4_1354at 1549.935 kb on - strand, within Pf1N1B4_1354at 1550.179 kb on - strand, within Pf1N1B4_1354at 1550.179 kb on - strand, within Pf1N1B4_1354at 1550.374 kb on - strand, within Pf1N1B4_1354at 1550.374 kb on - strand, within Pf1N1B4_1354at 1550.374 kb on - strand, within Pf1N1B4_1354at 1550.374 kb on - strand, within Pf1N1B4_1354at 1550.374 kb on - strand, within Pf1N1B4_1354at 1550.632 kb on - strand, within Pf1N1B4_1354at 1550.751 kb on + strand, within Pf1N1B4_1354at 1550.751 kb on + strand, within Pf1N1B4_1354at 1550.752 kb on - strand, within Pf1N1B4_1354at 1550.752 kb on - strand, within Pf1N1B4_1354at 1550.752 kb on - strand, within Pf1N1B4_1354at 1550.752 kb on - strand, within Pf1N1B4_1354at 1550.752 kb on - strand, within Pf1N1B4_1354at 1550.752 kb on - strand, within Pf1N1B4_1354at 1550.754 kb on - strand, within Pf1N1B4_1354at 1550.754 kb on - strand, within Pf1N1B4_1354at 1550.896 kb on + strand, within Pf1N1B4_1354at 1550.896 kb on + strand, within Pf1N1B4_1354at 1550.897 kb on - strand, within Pf1N1B4_1354at 1550.897 kb on - strand, within Pf1N1B4_1354at 1550.897 kb on - strand, within Pf1N1B4_1354at 1550.897 kb on - strand, within Pf1N1B4_1354at 1550.897 kb on - strand, within Pf1N1B4_1354at 1550.897 kb on - strand, within Pf1N1B4_1354at 1550.958 kb on - strand, within Pf1N1B4_1354at 1550.958 kb on - strand, within Pf1N1B4_1354at 1551.030 kb on - strand, within Pf1N1B4_1354at 1551.410 kb on - strand, within Pf1N1B4_1354at 1551.616 kb on - strand, within Pf1N1B4_1354at 1551.618 kb on - strand, within Pf1N1B4_1354at 1551.669 kb on + strand, within Pf1N1B4_1354at 1551.670 kb on - strand, within Pf1N1B4_1354at 1551.712 kb on - strand, within Pf1N1B4_1354at 1551.743 kb on + strand, within Pf1N1B4_1354at 1551.743 kb on + strand, within Pf1N1B4_1354at 1551.870 kb on + strand, within Pf1N1B4_1354at 1551.870 kb on + strand, within Pf1N1B4_1354at 1551.870 kb on + strand, within Pf1N1B4_1354at 1551.870 kb on + strand, within Pf1N1B4_1354at 1551.870 kb on + strand, within Pf1N1B4_1354at 1551.870 kb on + strand, within Pf1N1B4_1354at 1551.870 kb on + strand, within Pf1N1B4_1354at 1551.871 kb on - strand, within Pf1N1B4_1354at 1551.871 kb on - strand, within Pf1N1B4_1354at 1551.871 kb on - strand, within Pf1N1B4_1354at 1551.871 kb on - strand, within Pf1N1B4_1354at 1551.872 kb on + strand, within Pf1N1B4_1354at 1551.872 kb on + strand, within Pf1N1B4_1354at 1551.872 kb on + strand, within Pf1N1B4_1354at 1551.872 kb on + strand, within Pf1N1B4_1354at 1551.872 kb on + strand, within Pf1N1B4_1354at 1551.872 kb on + strand, within Pf1N1B4_1354at 1551.872 kb on + strand, within Pf1N1B4_1354at 1551.873 kb on - strand, within Pf1N1B4_1354at 1551.873 kb on - strand, within Pf1N1B4_1354at 1551.873 kb on - strand, within Pf1N1B4_1354at 1551.873 kb on - strand, within Pf1N1B4_1354at 1551.874 kb on - strand, within Pf1N1B4_1354at 1552.095 kb on + strandat 1552.110 kb on + strandat 1552.111 kb on - strandat 1552.111 kb on - strandat 1552.111 kb on - strandat 1552.111 kb on - strandat 1552.111 kb on - strandat 1552.112 kb on + strandat 1552.122 kb on + strandat 1552.123 kb on - strandat 1552.139 kb on + strandat 1552.139 kb on + strandat 1552.139 kb on + strandat 1552.139 kb on + strandat 1552.139 kb on + strandat 1552.140 kb on - strandat 1552.331 kb on + strandat 1552.480 kb on + strand, within Pf1N1B4_1355at 1552.480 kb on + strand, within Pf1N1B4_1355at 1552.481 kb on - strand, within Pf1N1B4_1355at 1552.481 kb on - strand, within Pf1N1B4_1355at 1552.521 kb on + strand, within Pf1N1B4_1355at 1552.521 kb on + strand, within Pf1N1B4_1355at 1552.521 kb on + strand, within Pf1N1B4_1355at 1552.521 kb on + strand, within Pf1N1B4_1355at 1552.521 kb on + strand, within Pf1N1B4_1355at 1552.521 kb on + strand, within Pf1N1B4_1355at 1552.521 kb on + strand, within Pf1N1B4_1355at 1552.521 kb on + strand, within Pf1N1B4_1355at 1552.522 kb on - strand, within Pf1N1B4_1355at 1552.522 kb on - strand, within Pf1N1B4_1355at 1552.522 kb on - strand, within Pf1N1B4_1355at 1552.642 kb on + strand, within Pf1N1B4_1355at 1552.729 kb on - strand, within Pf1N1B4_1355at 1552.729 kb on - strand, within Pf1N1B4_1355at 1552.742 kb on - strand, within Pf1N1B4_1355at 1552.788 kb on + strand, within Pf1N1B4_1355at 1552.816 kb on + strand, within Pf1N1B4_1355at 1552.817 kb on - strand, within Pf1N1B4_1355at 1552.817 kb on - strand, within Pf1N1B4_1355at 1552.955 kb on + strand, within Pf1N1B4_1355at 1552.956 kb on - strand, within Pf1N1B4_1355at 1552.956 kb on - strand, within Pf1N1B4_1355at 1553.021 kb on + strand, within Pf1N1B4_1355at 1553.120 kb on + strand, within Pf1N1B4_1355at 1553.121 kb on - strand, within Pf1N1B4_1355

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
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1,548,718 + Pf1N1B4_1353 0.47 +0.1
1,548,719 - Pf1N1B4_1353 0.48 -0.0
1,548,719 - Pf1N1B4_1353 0.48 -2.6
1,548,785 + Pf1N1B4_1353 0.51 -1.2
1,548,786 - Pf1N1B4_1353 0.51 -2.4
1,548,786 - Pf1N1B4_1353 0.51 -3.4
1,548,786 - Pf1N1B4_1353 0.51 -3.1
1,549,114 + Pf1N1B4_1353 0.71 +0.8
1,549,115 - Pf1N1B4_1353 0.71 -0.4
1,549,115 - Pf1N1B4_1353 0.71 -1.9
1,549,126 + Pf1N1B4_1353 0.71 -0.2
1,549,126 + Pf1N1B4_1353 0.71 +0.8
1,549,126 + Pf1N1B4_1353 0.71 +0.8
1,549,126 + Pf1N1B4_1353 0.71 -0.8
1,549,233 - Pf1N1B4_1353 0.78 +0.8
1,549,495 + +2.5
1,549,496 - +0.8
1,549,496 - -0.8
1,549,528 + -0.8
1,549,528 + -2.4
1,549,528 + +0.8
1,549,528 + +0.8
1,549,528 + +0.8
1,549,529 - +0.6
1,549,529 - -1.6
1,549,556 + -2.1
1,549,597 + +0.0
1,549,597 + -1.3
1,549,597 + +0.8
1,549,598 - +0.6
1,549,620 + +0.8
1,549,620 + -1.2
1,549,620 + +0.8
1,549,621 - -0.7
1,549,736 - +0.8
1,549,739 + -1.2
1,549,740 - -1.9
1,549,740 - +0.0
1,549,740 - -0.9
1,549,740 - -0.4
1,549,740 - -0.2
1,549,740 - -1.3
1,549,740 - +0.8
1,549,740 - +1.4
1,549,740 - -2.5
1,549,791 + +0.8
1,549,919 + +0.1
1,549,919 + +1.9
1,549,935 - Pf1N1B4_1354 0.10 +0.8
1,549,935 - Pf1N1B4_1354 0.10 -1.3
1,550,179 - Pf1N1B4_1354 0.20 -2.9
1,550,179 - Pf1N1B4_1354 0.20 -3.2
1,550,374 - Pf1N1B4_1354 0.28 -1.2
1,550,374 - Pf1N1B4_1354 0.28 -1.6
1,550,374 - Pf1N1B4_1354 0.28 -0.4
1,550,374 - Pf1N1B4_1354 0.28 -0.3
1,550,374 - Pf1N1B4_1354 0.28 -0.2
1,550,632 - Pf1N1B4_1354 0.39 -1.9
1,550,751 + Pf1N1B4_1354 0.44 -2.9
1,550,751 + Pf1N1B4_1354 0.44 +0.0
1,550,752 - Pf1N1B4_1354 0.44 -1.3
1,550,752 - Pf1N1B4_1354 0.44 +0.8
1,550,752 - Pf1N1B4_1354 0.44 -1.2
1,550,752 - Pf1N1B4_1354 0.44 -1.2
1,550,752 - Pf1N1B4_1354 0.44 -3.5
1,550,752 - Pf1N1B4_1354 0.44 -2.4
1,550,754 - Pf1N1B4_1354 0.44 +0.8
1,550,754 - Pf1N1B4_1354 0.44 -1.0
1,550,896 + Pf1N1B4_1354 0.50 +0.8
1,550,896 + Pf1N1B4_1354 0.50 -1.0
1,550,897 - Pf1N1B4_1354 0.50 -2.9
1,550,897 - Pf1N1B4_1354 0.50 +0.8
1,550,897 - Pf1N1B4_1354 0.50 +0.2
1,550,897 - Pf1N1B4_1354 0.50 -1.8
1,550,897 - Pf1N1B4_1354 0.50 -1.8
1,550,897 - Pf1N1B4_1354 0.50 +0.5
1,550,958 - Pf1N1B4_1354 0.52 -0.2
1,550,958 - Pf1N1B4_1354 0.52 +0.8
1,551,030 - Pf1N1B4_1354 0.55 -0.8
1,551,410 - Pf1N1B4_1354 0.70 -1.8
1,551,616 - Pf1N1B4_1354 0.79 -0.1
1,551,618 - Pf1N1B4_1354 0.79 -0.4
1,551,669 + Pf1N1B4_1354 0.81 -0.4
1,551,670 - Pf1N1B4_1354 0.81 -1.4
1,551,712 - Pf1N1B4_1354 0.83 -1.1
1,551,743 + Pf1N1B4_1354 0.84 -0.5
1,551,743 + Pf1N1B4_1354 0.84 -1.1
1,551,870 + Pf1N1B4_1354 0.89 -1.1
1,551,870 + Pf1N1B4_1354 0.89 -0.9
1,551,870 + Pf1N1B4_1354 0.89 -0.6
1,551,870 + Pf1N1B4_1354 0.89 -0.9
1,551,870 + Pf1N1B4_1354 0.89 -0.1
1,551,870 + Pf1N1B4_1354 0.89 +0.5
1,551,870 + Pf1N1B4_1354 0.89 +1.3
1,551,871 - Pf1N1B4_1354 0.89 +0.2
1,551,871 - Pf1N1B4_1354 0.89 +0.8
1,551,871 - Pf1N1B4_1354 0.89 -1.8
1,551,871 - Pf1N1B4_1354 0.89 -0.6
1,551,872 + Pf1N1B4_1354 0.89 -1.6
1,551,872 + Pf1N1B4_1354 0.89 -0.6
1,551,872 + Pf1N1B4_1354 0.89 -0.4
1,551,872 + Pf1N1B4_1354 0.89 -0.5
1,551,872 + Pf1N1B4_1354 0.89 -2.8
1,551,872 + Pf1N1B4_1354 0.89 +0.8
1,551,872 + Pf1N1B4_1354 0.89 -0.3
1,551,873 - Pf1N1B4_1354 0.89 +0.2
1,551,873 - Pf1N1B4_1354 0.89 -0.2
1,551,873 - Pf1N1B4_1354 0.89 -1.7
1,551,873 - Pf1N1B4_1354 0.89 +1.9
1,551,874 - Pf1N1B4_1354 0.89 -2.2
1,552,095 + -2.3
1,552,110 + +0.8
1,552,111 - -0.2
1,552,111 - -1.2
1,552,111 - +1.8
1,552,111 - +0.0
1,552,111 - -2.4
1,552,112 + -1.2
1,552,122 + +0.8
1,552,123 - -2.5
1,552,139 + -1.2
1,552,139 + -1.5
1,552,139 + -0.6
1,552,139 + -2.0
1,552,139 + +0.8
1,552,140 - +0.8
1,552,331 + +0.8
1,552,480 + Pf1N1B4_1355 0.12 -1.5
1,552,480 + Pf1N1B4_1355 0.12 -0.2
1,552,481 - Pf1N1B4_1355 0.12 +3.4
1,552,481 - Pf1N1B4_1355 0.12 -4.0
1,552,521 + Pf1N1B4_1355 0.13 -0.4
1,552,521 + Pf1N1B4_1355 0.13 +0.8
1,552,521 + Pf1N1B4_1355 0.13 +0.8
1,552,521 + Pf1N1B4_1355 0.13 -2.5
1,552,521 + Pf1N1B4_1355 0.13 -0.6
1,552,521 + Pf1N1B4_1355 0.13 +0.8
1,552,521 + Pf1N1B4_1355 0.13 -2.5
1,552,521 + Pf1N1B4_1355 0.13 -0.5
1,552,522 - Pf1N1B4_1355 0.13 -2.4
1,552,522 - Pf1N1B4_1355 0.13 -0.2
1,552,522 - Pf1N1B4_1355 0.13 -0.6
1,552,642 + Pf1N1B4_1355 0.18 +0.8
1,552,729 - Pf1N1B4_1355 0.21 +0.8
1,552,729 - Pf1N1B4_1355 0.21 -0.2
1,552,742 - Pf1N1B4_1355 0.21 -0.6
1,552,788 + Pf1N1B4_1355 0.23 -1.1
1,552,816 + Pf1N1B4_1355 0.24 +0.8
1,552,817 - Pf1N1B4_1355 0.24 +1.2
1,552,817 - Pf1N1B4_1355 0.24 -1.2
1,552,955 + Pf1N1B4_1355 0.30 -0.4
1,552,956 - Pf1N1B4_1355 0.30 +0.1
1,552,956 - Pf1N1B4_1355 0.30 -1.3
1,553,021 + Pf1N1B4_1355 0.32 +0.8
1,553,120 + Pf1N1B4_1355 0.36 -2.5
1,553,121 - Pf1N1B4_1355 0.36 +0.8

Or see this region's nucleotide sequence