Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1256 and Pf1N1B4_1257 are separated by 117 nucleotides Pf1N1B4_1257 and Pf1N1B4_1258 are separated by 274 nucleotides Pf1N1B4_1258 and Pf1N1B4_1259 are separated by 8 nucleotides
Pf1N1B4_1256 - Transcriptional regulator, LysR family, at 1,437,176 to 1,438,105
_1256
Pf1N1B4_1257 - Bifunctional protein: zinc-containing alcohol dehydrogenase; quinone oxidoreductase ( NADPH:quinone reductase) (EC 1.1.1.-); Similar to arginate lyase, at 1,438,223 to 1,439,236
_1257
Pf1N1B4_1258 - FIG00956405: hypothetical protein, at 1,439,511 to 1,439,681
_1258
Pf1N1B4_1259 - Multidrug resistance transporter, Bcr/CflA family, at 1,439,690 to 1,440,886
_1259
Position (kb)
1438
1439
1440 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1437.369 kb on - strand, within Pf1N1B4_1256 at 1437.385 kb on + strand, within Pf1N1B4_1256 at 1437.385 kb on + strand, within Pf1N1B4_1256 at 1437.386 kb on - strand, within Pf1N1B4_1256 at 1437.530 kb on + strand, within Pf1N1B4_1256 at 1437.611 kb on + strand, within Pf1N1B4_1256 at 1437.673 kb on - strand, within Pf1N1B4_1256 at 1437.903 kb on + strand, within Pf1N1B4_1256 at 1438.024 kb on + strand at 1438.024 kb on + strand at 1438.024 kb on + strand at 1438.024 kb on + strand at 1438.025 kb on - strand at 1438.161 kb on + strand at 1438.162 kb on - strand at 1438.162 kb on - strand at 1438.222 kb on + strand at 1438.223 kb on - strand at 1438.223 kb on - strand at 1438.238 kb on + strand at 1438.238 kb on + strand at 1438.238 kb on + strand at 1438.239 kb on - strand at 1438.239 kb on - strand at 1438.239 kb on - strand at 1438.241 kb on + strand at 1438.242 kb on - strand at 1438.242 kb on - strand at 1438.487 kb on + strand, within Pf1N1B4_1257 at 1438.558 kb on + strand at 1438.558 kb on + strand, within Pf1N1B4_1257 at 1439.033 kb on + strand, within Pf1N1B4_1257 at 1439.033 kb on + strand, within Pf1N1B4_1257 at 1439.034 kb on - strand, within Pf1N1B4_1257 at 1439.034 kb on - strand, within Pf1N1B4_1257 at 1439.034 kb on - strand, within Pf1N1B4_1257 at 1439.034 kb on - strand, within Pf1N1B4_1257 at 1439.034 kb on - strand, within Pf1N1B4_1257 at 1439.034 kb on - strand, within Pf1N1B4_1257 at 1439.034 kb on - strand at 1439.249 kb on + strand at 1439.290 kb on - strand at 1439.342 kb on + strand at 1439.342 kb on + strand at 1439.342 kb on + strand at 1439.343 kb on - strand at 1439.343 kb on - strand at 1439.366 kb on + strand at 1439.366 kb on + strand at 1439.366 kb on + strand at 1439.366 kb on + strand at 1439.366 kb on + strand at 1439.366 kb on + strand at 1439.366 kb on + strand at 1439.367 kb on - strand at 1439.367 kb on - strand at 1439.367 kb on - strand at 1439.426 kb on + strand at 1439.426 kb on + strand at 1439.427 kb on - strand at 1439.437 kb on - strand at 1439.465 kb on + strand at 1439.465 kb on + strand at 1439.465 kb on + strand at 1439.466 kb on - strand at 1439.466 kb on - strand at 1439.466 kb on - strand at 1439.466 kb on - strand at 1439.479 kb on + strand at 1439.479 kb on + strand at 1439.493 kb on + strand at 1439.494 kb on - strand at 1439.494 kb on - strand at 1439.524 kb on + strand at 1439.613 kb on - strand, within Pf1N1B4_1258 at 1439.613 kb on - strand, within Pf1N1B4_1258 at 1439.813 kb on + strand, within Pf1N1B4_1259 at 1439.814 kb on - strand at 1439.814 kb on - strand, within Pf1N1B4_1259 at 1439.936 kb on + strand, within Pf1N1B4_1259 at 1439.937 kb on - strand, within Pf1N1B4_1259 at 1439.937 kb on - strand, within Pf1N1B4_1259 at 1440.042 kb on + strand, within Pf1N1B4_1259 at 1440.042 kb on + strand at 1440.042 kb on + strand, within Pf1N1B4_1259 at 1440.043 kb on - strand, within Pf1N1B4_1259 at 1440.043 kb on - strand, within Pf1N1B4_1259 at 1440.043 kb on - strand at 1440.177 kb on + strand, within Pf1N1B4_1259 at 1440.178 kb on - strand, within Pf1N1B4_1259 at 1440.178 kb on - strand, within Pf1N1B4_1259 at 1440.213 kb on + strand, within Pf1N1B4_1259 at 1440.213 kb on + strand, within Pf1N1B4_1259 at 1440.214 kb on - strand, within Pf1N1B4_1259
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 1,437,369 - Pf1N1B4_1256 0.21 -1.0 1,437,385 + Pf1N1B4_1256 0.22 -2.0 1,437,385 + Pf1N1B4_1256 0.22 -1.0 1,437,386 - Pf1N1B4_1256 0.23 -1.2 1,437,530 + Pf1N1B4_1256 0.38 +0.8 1,437,611 + Pf1N1B4_1256 0.47 -3.0 1,437,673 - Pf1N1B4_1256 0.53 +0.8 1,437,903 + Pf1N1B4_1256 0.78 +0.1 1,438,024 + +0.8 1,438,024 + +0.8 1,438,024 + +0.6 1,438,024 + +0.2 1,438,025 - -0.5 1,438,161 + -2.2 1,438,162 - -0.3 1,438,162 - -1.8 1,438,222 + +0.8 1,438,223 - -0.4 1,438,223 - -0.5 1,438,238 + +0.2 1,438,238 + -2.7 1,438,238 + +0.8 1,438,239 - +0.2 1,438,239 - -1.7 1,438,239 - -2.2 1,438,241 + +1.3 1,438,242 - +0.8 1,438,242 - -1.0 1,438,487 + Pf1N1B4_1257 0.26 +0.8 1,438,558 + +0.2 1,438,558 + Pf1N1B4_1257 0.33 +0.8 1,439,033 + Pf1N1B4_1257 0.80 -0.0 1,439,033 + Pf1N1B4_1257 0.80 -0.4 1,439,034 - Pf1N1B4_1257 0.80 -3.1 1,439,034 - Pf1N1B4_1257 0.80 +0.2 1,439,034 - Pf1N1B4_1257 0.80 +0.8 1,439,034 - Pf1N1B4_1257 0.80 +0.3 1,439,034 - Pf1N1B4_1257 0.80 +0.9 1,439,034 - Pf1N1B4_1257 0.80 -2.4 1,439,034 - -1.6 1,439,249 + -1.2 1,439,290 - +0.8 1,439,342 + -2.7 1,439,342 + -1.6 1,439,342 + -0.8 1,439,343 - +1.1 1,439,343 - -3.5 1,439,366 + +0.8 1,439,366 + -2.0 1,439,366 + +0.2 1,439,366 + +0.8 1,439,366 + -0.8 1,439,366 + +0.8 1,439,366 + -2.2 1,439,367 - +1.3 1,439,367 - -1.7 1,439,367 - -0.9 1,439,426 + -0.7 1,439,426 + -2.4 1,439,427 - -2.5 1,439,437 - +0.8 1,439,465 + -2.0 1,439,465 + -0.0 1,439,465 + -2.0 1,439,466 - +0.6 1,439,466 - -1.0 1,439,466 - -1.0 1,439,466 - +0.8 1,439,479 + -1.2 1,439,479 + +0.4 1,439,493 + +0.8 1,439,494 - +0.8 1,439,494 - -1.0 1,439,524 + -0.8 1,439,613 - Pf1N1B4_1258 0.60 +1.4 1,439,613 - Pf1N1B4_1258 0.60 -1.6 1,439,813 + Pf1N1B4_1259 0.10 -2.7 1,439,814 - -2.2 1,439,814 - Pf1N1B4_1259 0.10 -1.8 1,439,936 + Pf1N1B4_1259 0.21 +0.5 1,439,937 - Pf1N1B4_1259 0.21 -0.4 1,439,937 - Pf1N1B4_1259 0.21 +0.8 1,440,042 + Pf1N1B4_1259 0.29 +0.8 1,440,042 + -2.0 1,440,042 + Pf1N1B4_1259 0.29 +0.8 1,440,043 - Pf1N1B4_1259 0.29 +0.5 1,440,043 - Pf1N1B4_1259 0.29 -2.1 1,440,043 - -0.7 1,440,177 + Pf1N1B4_1259 0.41 -1.4 1,440,178 - Pf1N1B4_1259 0.41 -2.0 1,440,178 - Pf1N1B4_1259 0.41 +0.4 1,440,213 + Pf1N1B4_1259 0.44 +0.2 1,440,213 + Pf1N1B4_1259 0.44 -1.4 1,440,214 - Pf1N1B4_1259 0.44 +0.3
Or see this region's nucleotide sequence