Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_119 and Pf1N1B4_120 are separated by 92 nucleotides Pf1N1B4_120 and Pf1N1B4_121 are separated by 233 nucleotides
Pf1N1B4_119 - Putative haemolysin/cytolysin secreted via TPS pathway, at 125,607 to 130,118
_119
Pf1N1B4_120 - Channel-forming transporter/cytolysins activator of TpsB family, at 130,211 to 131,938
_120
Pf1N1B4_121 - Membrane fusion component of tripartite multidrug resistance system, at 132,172 to 133,293
_121
Position (kb)
130
131
132 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 129.286 kb on - strand, within Pf1N1B4_119 at 129.703 kb on + strand at 129.703 kb on + strand at 129.704 kb on - strand at 129.704 kb on - strand at 129.704 kb on - strand at 129.704 kb on - strand at 129.704 kb on - strand at 129.892 kb on + strand at 129.893 kb on - strand at 129.893 kb on - strand at 130.134 kb on - strand at 130.291 kb on - strand at 130.456 kb on + strand at 130.569 kb on + strand, within Pf1N1B4_120 at 130.650 kb on + strand, within Pf1N1B4_120 at 130.734 kb on + strand, within Pf1N1B4_120 at 130.843 kb on - strand, within Pf1N1B4_120 at 131.020 kb on - strand, within Pf1N1B4_120 at 131.047 kb on - strand, within Pf1N1B4_120 at 131.050 kb on - strand, within Pf1N1B4_120 at 131.514 kb on + strand, within Pf1N1B4_120 at 131.514 kb on + strand, within Pf1N1B4_120 at 131.529 kb on + strand at 131.529 kb on + strand, within Pf1N1B4_120 at 131.529 kb on + strand, within Pf1N1B4_120 at 131.994 kb on - strand at 132.031 kb on - strand at 132.031 kb on - strand at 132.163 kb on + strand at 132.163 kb on + strand at 132.164 kb on - strand at 132.164 kb on - strand at 132.267 kb on + strand at 132.349 kb on + strand, within Pf1N1B4_121 at 132.349 kb on + strand, within Pf1N1B4_121 at 132.350 kb on - strand, within Pf1N1B4_121 at 132.524 kb on + strand, within Pf1N1B4_121 at 132.641 kb on - strand, within Pf1N1B4_121 at 132.856 kb on + strand, within Pf1N1B4_121
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 129,286 - Pf1N1B4_119 0.82 -2.5 129,703 + +0.7 129,703 + -0.3 129,704 - -2.1 129,704 - -1.0 129,704 - +0.7 129,704 - -1.0 129,704 - -0.1 129,892 + -2.6 129,893 - -2.9 129,893 - -0.3 130,134 - +0.3 130,291 - -2.9 130,456 + -2.8 130,569 + Pf1N1B4_120 0.21 -1.3 130,650 + Pf1N1B4_120 0.25 +0.7 130,734 + Pf1N1B4_120 0.30 +0.3 130,843 - Pf1N1B4_120 0.37 +0.7 131,020 - Pf1N1B4_120 0.47 -0.4 131,047 - Pf1N1B4_120 0.48 -0.0 131,050 - Pf1N1B4_120 0.49 +2.8 131,514 + Pf1N1B4_120 0.75 +0.7 131,514 + Pf1N1B4_120 0.75 -1.6 131,529 + +0.7 131,529 + Pf1N1B4_120 0.76 +0.4 131,529 + Pf1N1B4_120 0.76 +0.7 131,994 - -1.8 132,031 - +0.7 132,031 - +0.7 132,163 + +0.7 132,163 + +1.1 132,164 - +0.1 132,164 - +0.7 132,267 + +0.7 132,349 + Pf1N1B4_121 0.16 +0.7 132,349 + Pf1N1B4_121 0.16 -0.1 132,350 - Pf1N1B4_121 0.16 -1.4 132,524 + Pf1N1B4_121 0.31 +0.7 132,641 - Pf1N1B4_121 0.42 +0.2 132,856 + Pf1N1B4_121 0.61 -1.2
Or see this region's nucleotide sequence