Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_120

Experiment: N2E2wt with N1B4m denitrifying repB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_119 and Pf1N1B4_120 are separated by 92 nucleotidesPf1N1B4_120 and Pf1N1B4_121 are separated by 233 nucleotides Pf1N1B4_119 - Putative haemolysin/cytolysin secreted via TPS pathway, at 125,607 to 130,118 _119 Pf1N1B4_120 - Channel-forming transporter/cytolysins activator of TpsB family, at 130,211 to 131,938 _120 Pf1N1B4_121 - Membrane fusion component of tripartite multidrug resistance system, at 132,172 to 133,293 _121 Position (kb) 130 131 132Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 129.286 kb on - strand, within Pf1N1B4_119at 129.703 kb on + strandat 129.703 kb on + strandat 129.704 kb on - strandat 129.704 kb on - strandat 129.704 kb on - strandat 129.704 kb on - strandat 129.704 kb on - strandat 129.892 kb on + strandat 129.893 kb on - strandat 129.893 kb on - strandat 130.134 kb on - strandat 130.291 kb on - strandat 130.456 kb on + strandat 130.569 kb on + strand, within Pf1N1B4_120at 130.650 kb on + strand, within Pf1N1B4_120at 130.734 kb on + strand, within Pf1N1B4_120at 130.843 kb on - strand, within Pf1N1B4_120at 131.020 kb on - strand, within Pf1N1B4_120at 131.047 kb on - strand, within Pf1N1B4_120at 131.050 kb on - strand, within Pf1N1B4_120at 131.514 kb on + strand, within Pf1N1B4_120at 131.514 kb on + strand, within Pf1N1B4_120at 131.529 kb on + strandat 131.529 kb on + strand, within Pf1N1B4_120at 131.529 kb on + strand, within Pf1N1B4_120at 131.994 kb on - strandat 132.031 kb on - strandat 132.031 kb on - strandat 132.163 kb on + strandat 132.163 kb on + strandat 132.164 kb on - strandat 132.164 kb on - strandat 132.267 kb on + strandat 132.349 kb on + strand, within Pf1N1B4_121at 132.349 kb on + strand, within Pf1N1B4_121at 132.350 kb on - strand, within Pf1N1B4_121at 132.524 kb on + strand, within Pf1N1B4_121at 132.641 kb on - strand, within Pf1N1B4_121at 132.856 kb on + strand, within Pf1N1B4_121

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
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129,286 - Pf1N1B4_119 0.82 -2.5
129,703 + +0.7
129,703 + -0.3
129,704 - -2.1
129,704 - -1.0
129,704 - +0.7
129,704 - -1.0
129,704 - -0.1
129,892 + -2.6
129,893 - -2.9
129,893 - -0.3
130,134 - +0.3
130,291 - -2.9
130,456 + -2.8
130,569 + Pf1N1B4_120 0.21 -1.3
130,650 + Pf1N1B4_120 0.25 +0.7
130,734 + Pf1N1B4_120 0.30 +0.3
130,843 - Pf1N1B4_120 0.37 +0.7
131,020 - Pf1N1B4_120 0.47 -0.4
131,047 - Pf1N1B4_120 0.48 -0.0
131,050 - Pf1N1B4_120 0.49 +2.8
131,514 + Pf1N1B4_120 0.75 +0.7
131,514 + Pf1N1B4_120 0.75 -1.6
131,529 + +0.7
131,529 + Pf1N1B4_120 0.76 +0.4
131,529 + Pf1N1B4_120 0.76 +0.7
131,994 - -1.8
132,031 - +0.7
132,031 - +0.7
132,163 + +0.7
132,163 + +1.1
132,164 - +0.1
132,164 - +0.7
132,267 + +0.7
132,349 + Pf1N1B4_121 0.16 +0.7
132,349 + Pf1N1B4_121 0.16 -0.1
132,350 - Pf1N1B4_121 0.16 -1.4
132,524 + Pf1N1B4_121 0.31 +0.7
132,641 - Pf1N1B4_121 0.42 +0.2
132,856 + Pf1N1B4_121 0.61 -1.2

Or see this region's nucleotide sequence