Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1132 and Pf1N1B4_1133 are separated by 12 nucleotides Pf1N1B4_1133 and Pf1N1B4_1134 overlap by 4 nucleotides Pf1N1B4_1134 and Pf1N1B4_1135 are separated by 179 nucleotides Pf1N1B4_1135 and Pf1N1B4_1136 are separated by 134 nucleotides
Pf1N1B4_1132 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) / Osmotic adaptation, at 1,270,262 to 1,271,155
_1132
Pf1N1B4_1133 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,271,168 to 1,271,821
_1133
Pf1N1B4_1134 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 1,271,818 to 1,272,975
_1134
Pf1N1B4_1135 - FIG00960055: hypothetical protein, at 1,273,155 to 1,273,307
_1135
Pf1N1B4_1136 - Ornithine decarboxylase (EC 4.1.1.17) / Arginine decarboxylase (EC 4.1.1.19), at 1,273,442 to 1,274,605
_1136
Position (kb)
1271
1272
1273 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1270.854 kb on - strand, within Pf1N1B4_1132 at 1270.854 kb on - strand, within Pf1N1B4_1132 at 1270.896 kb on - strand at 1270.896 kb on - strand, within Pf1N1B4_1132 at 1270.896 kb on - strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.998 kb on - strand, within Pf1N1B4_1132 at 1271.396 kb on + strand, within Pf1N1B4_1133 at 1271.397 kb on - strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.679 kb on + strand, within Pf1N1B4_1133 at 1271.679 kb on + strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1272.054 kb on + strand at 1272.054 kb on + strand, within Pf1N1B4_1134 at 1272.055 kb on - strand, within Pf1N1B4_1134 at 1272.055 kb on - strand, within Pf1N1B4_1134 at 1272.064 kb on + strand, within Pf1N1B4_1134 at 1272.065 kb on - strand, within Pf1N1B4_1134 at 1272.065 kb on - strand, within Pf1N1B4_1134 at 1272.211 kb on + strand, within Pf1N1B4_1134 at 1272.556 kb on - strand, within Pf1N1B4_1134 at 1272.667 kb on + strand, within Pf1N1B4_1134 at 1272.706 kb on + strand, within Pf1N1B4_1134 at 1272.754 kb on + strand, within Pf1N1B4_1134 at 1272.755 kb on - strand, within Pf1N1B4_1134 at 1272.755 kb on - strand, within Pf1N1B4_1134 at 1272.784 kb on + strand, within Pf1N1B4_1134 at 1272.784 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.802 kb on - strand, within Pf1N1B4_1134 at 1272.820 kb on + strand, within Pf1N1B4_1134 at 1272.820 kb on + strand, within Pf1N1B4_1134 at 1272.820 kb on + strand, within Pf1N1B4_1134 at 1272.820 kb on + strand, within Pf1N1B4_1134 at 1272.821 kb on - strand, within Pf1N1B4_1134 at 1272.821 kb on - strand, within Pf1N1B4_1134 at 1272.904 kb on + strand at 1273.021 kb on - strand at 1273.021 kb on - strand at 1273.036 kb on - strand at 1273.111 kb on - strand at 1273.120 kb on - strand at 1273.151 kb on + strand at 1273.152 kb on - strand at 1273.152 kb on - strand at 1273.152 kb on - strand at 1273.152 kb on - strand at 1273.152 kb on - strand at 1273.152 kb on - strand at 1273.159 kb on + strand at 1273.159 kb on + strand at 1273.159 kb on + strand at 1273.159 kb on + strand at 1273.160 kb on - strand at 1273.160 kb on - strand at 1273.160 kb on - strand at 1273.283 kb on - strand, within Pf1N1B4_1135 at 1273.283 kb on - strand, within Pf1N1B4_1135 at 1273.283 kb on - strand, within Pf1N1B4_1135 at 1273.283 kb on - strand, within Pf1N1B4_1135 at 1273.283 kb on - strand, within Pf1N1B4_1135 at 1273.362 kb on + strand at 1273.362 kb on + strand at 1273.412 kb on - strand at 1273.464 kb on - strand at 1273.599 kb on - strand, within Pf1N1B4_1136 at 1273.599 kb on - strand, within Pf1N1B4_1136 at 1273.599 kb on - strand, within Pf1N1B4_1136 at 1273.599 kb on - strand, within Pf1N1B4_1136 at 1273.601 kb on + strand, within Pf1N1B4_1136 at 1273.602 kb on - strand, within Pf1N1B4_1136 at 1273.682 kb on + strand, within Pf1N1B4_1136 at 1273.683 kb on - strand, within Pf1N1B4_1136 at 1273.770 kb on - strand, within Pf1N1B4_1136 at 1273.776 kb on - strand, within Pf1N1B4_1136 at 1273.796 kb on + strand, within Pf1N1B4_1136 at 1273.796 kb on + strand, within Pf1N1B4_1136 at 1273.797 kb on - strand, within Pf1N1B4_1136 at 1273.859 kb on + strand, within Pf1N1B4_1136 at 1273.860 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 1,270,854 - Pf1N1B4_1132 0.66 +0.8 1,270,854 - Pf1N1B4_1132 0.66 +0.8 1,270,896 - -0.8 1,270,896 - Pf1N1B4_1132 0.71 +0.9 1,270,896 - Pf1N1B4_1132 0.71 -0.8 1,270,955 + Pf1N1B4_1132 0.78 +0.8 1,270,955 + Pf1N1B4_1132 0.78 +0.8 1,270,955 + Pf1N1B4_1132 0.78 +1.2 1,270,955 + Pf1N1B4_1132 0.78 +0.8 1,270,955 + -3.1 1,270,955 + Pf1N1B4_1132 0.78 -1.2 1,270,955 + Pf1N1B4_1132 0.78 +0.8 1,270,955 + Pf1N1B4_1132 0.78 +0.9 1,270,955 + Pf1N1B4_1132 0.78 +0.8 1,270,956 - Pf1N1B4_1132 0.78 +0.8 1,270,956 - Pf1N1B4_1132 0.78 -1.6 1,270,956 - Pf1N1B4_1132 0.78 +0.8 1,270,956 - Pf1N1B4_1132 0.78 -0.2 1,270,967 + Pf1N1B4_1132 0.79 -1.3 1,270,967 + Pf1N1B4_1132 0.79 +0.3 1,270,967 + Pf1N1B4_1132 0.79 +0.8 1,270,998 - Pf1N1B4_1132 0.82 -2.4 1,271,396 + Pf1N1B4_1133 0.35 +0.1 1,271,397 - Pf1N1B4_1133 0.35 +0.8 1,271,483 + Pf1N1B4_1133 0.48 +0.1 1,271,483 + +0.8 1,271,483 + Pf1N1B4_1133 0.48 -2.2 1,271,483 + Pf1N1B4_1133 0.48 -0.2 1,271,483 + Pf1N1B4_1133 0.48 -1.8 1,271,484 - Pf1N1B4_1133 0.48 -0.3 1,271,484 - Pf1N1B4_1133 0.48 -0.2 1,271,484 - Pf1N1B4_1133 0.48 -0.9 1,271,484 - Pf1N1B4_1133 0.48 -1.2 1,271,484 - Pf1N1B4_1133 0.48 -0.8 1,271,484 - Pf1N1B4_1133 0.48 -1.6 1,271,679 + Pf1N1B4_1133 0.78 -2.4 1,271,679 + Pf1N1B4_1133 0.78 -1.8 1,271,680 - Pf1N1B4_1133 0.78 +2.4 1,271,680 - Pf1N1B4_1133 0.78 -2.0 1,271,680 - Pf1N1B4_1133 0.78 +0.8 1,272,054 + -0.7 1,272,054 + Pf1N1B4_1134 0.20 -0.2 1,272,055 - Pf1N1B4_1134 0.20 +0.8 1,272,055 - Pf1N1B4_1134 0.20 +0.8 1,272,064 + Pf1N1B4_1134 0.21 +1.2 1,272,065 - Pf1N1B4_1134 0.21 -2.8 1,272,065 - Pf1N1B4_1134 0.21 -0.8 1,272,211 + Pf1N1B4_1134 0.34 +0.8 1,272,556 - Pf1N1B4_1134 0.64 -1.5 1,272,667 + Pf1N1B4_1134 0.73 -1.5 1,272,706 + Pf1N1B4_1134 0.77 +0.2 1,272,754 + Pf1N1B4_1134 0.81 -0.6 1,272,755 - Pf1N1B4_1134 0.81 -1.0 1,272,755 - Pf1N1B4_1134 0.81 +0.8 1,272,784 + Pf1N1B4_1134 0.83 +0.6 1,272,784 + Pf1N1B4_1134 0.83 +0.8 1,272,797 + Pf1N1B4_1134 0.85 -0.5 1,272,797 + Pf1N1B4_1134 0.85 -0.2 1,272,797 + Pf1N1B4_1134 0.85 +0.4 1,272,797 + Pf1N1B4_1134 0.85 -4.0 1,272,797 + Pf1N1B4_1134 0.85 +0.8 1,272,797 + Pf1N1B4_1134 0.85 -0.2 1,272,797 + Pf1N1B4_1134 0.85 -0.8 1,272,797 + Pf1N1B4_1134 0.85 +0.8 1,272,797 + Pf1N1B4_1134 0.85 +2.7 1,272,797 + Pf1N1B4_1134 0.85 +0.2 1,272,797 + Pf1N1B4_1134 0.85 -1.1 1,272,798 - Pf1N1B4_1134 0.85 +0.8 1,272,798 - Pf1N1B4_1134 0.85 +0.2 1,272,798 - Pf1N1B4_1134 0.85 -1.1 1,272,798 - Pf1N1B4_1134 0.85 -0.2 1,272,798 - Pf1N1B4_1134 0.85 +1.1 1,272,798 - Pf1N1B4_1134 0.85 +0.8 1,272,798 - Pf1N1B4_1134 0.85 +0.8 1,272,801 + Pf1N1B4_1134 0.85 +0.8 1,272,801 + Pf1N1B4_1134 0.85 -2.1 1,272,801 + Pf1N1B4_1134 0.85 +0.8 1,272,801 + Pf1N1B4_1134 0.85 -0.1 1,272,801 + Pf1N1B4_1134 0.85 +0.8 1,272,802 - Pf1N1B4_1134 0.85 -1.2 1,272,820 + Pf1N1B4_1134 0.87 -3.5 1,272,820 + Pf1N1B4_1134 0.87 +0.8 1,272,820 + Pf1N1B4_1134 0.87 -0.2 1,272,820 + Pf1N1B4_1134 0.87 -0.7 1,272,821 - Pf1N1B4_1134 0.87 -0.9 1,272,821 - Pf1N1B4_1134 0.87 +0.2 1,272,904 + -0.8 1,273,021 - +0.7 1,273,021 - +0.8 1,273,036 - -0.6 1,273,111 - +0.2 1,273,120 - -0.1 1,273,151 + -0.7 1,273,152 - -0.6 1,273,152 - -0.6 1,273,152 - +0.4 1,273,152 - -0.2 1,273,152 - +0.8 1,273,152 - +0.8 1,273,159 + -0.0 1,273,159 + -1.0 1,273,159 + -1.3 1,273,159 + -2.2 1,273,160 - -0.7 1,273,160 - -3.7 1,273,160 - +0.8 1,273,283 - Pf1N1B4_1135 0.84 -0.7 1,273,283 - Pf1N1B4_1135 0.84 -2.5 1,273,283 - Pf1N1B4_1135 0.84 -4.0 1,273,283 - Pf1N1B4_1135 0.84 +0.8 1,273,283 - Pf1N1B4_1135 0.84 -3.3 1,273,362 + +0.8 1,273,362 + -1.8 1,273,412 - -1.2 1,273,464 - -2.3 1,273,599 - Pf1N1B4_1136 0.13 -1.8 1,273,599 - Pf1N1B4_1136 0.13 -2.4 1,273,599 - Pf1N1B4_1136 0.13 -0.0 1,273,599 - Pf1N1B4_1136 0.13 -1.2 1,273,601 + Pf1N1B4_1136 0.14 +0.9 1,273,602 - Pf1N1B4_1136 0.14 +0.8 1,273,682 + Pf1N1B4_1136 0.21 -0.8 1,273,683 - Pf1N1B4_1136 0.21 +0.8 1,273,770 - Pf1N1B4_1136 0.28 +0.8 1,273,776 - Pf1N1B4_1136 0.29 -0.6 1,273,796 + Pf1N1B4_1136 0.30 -3.5 1,273,796 + Pf1N1B4_1136 0.30 -0.1 1,273,797 - Pf1N1B4_1136 0.30 +0.8 1,273,859 + Pf1N1B4_1136 0.36 -2.0 1,273,860 - -2.2
Or see this region's nucleotide sequence