Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1134

Experiment: N2E2wt with N1B4m denitrifying repB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1132 and Pf1N1B4_1133 are separated by 12 nucleotidesPf1N1B4_1133 and Pf1N1B4_1134 overlap by 4 nucleotidesPf1N1B4_1134 and Pf1N1B4_1135 are separated by 179 nucleotidesPf1N1B4_1135 and Pf1N1B4_1136 are separated by 134 nucleotides Pf1N1B4_1132 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) / Osmotic adaptation, at 1,270,262 to 1,271,155 _1132 Pf1N1B4_1133 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,271,168 to 1,271,821 _1133 Pf1N1B4_1134 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 1,271,818 to 1,272,975 _1134 Pf1N1B4_1135 - FIG00960055: hypothetical protein, at 1,273,155 to 1,273,307 _1135 Pf1N1B4_1136 - Ornithine decarboxylase (EC 4.1.1.17) / Arginine decarboxylase (EC 4.1.1.19), at 1,273,442 to 1,274,605 _1136 Position (kb) 1271 1272 1273Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1270.854 kb on - strand, within Pf1N1B4_1132at 1270.854 kb on - strand, within Pf1N1B4_1132at 1270.896 kb on - strandat 1270.896 kb on - strand, within Pf1N1B4_1132at 1270.896 kb on - strand, within Pf1N1B4_1132at 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.955 kb on + strandat 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.955 kb on + strand, within Pf1N1B4_1132at 1270.956 kb on - strand, within Pf1N1B4_1132at 1270.956 kb on - strand, within Pf1N1B4_1132at 1270.956 kb on - strand, within Pf1N1B4_1132at 1270.956 kb on - strand, within Pf1N1B4_1132at 1270.967 kb on + strand, within Pf1N1B4_1132at 1270.967 kb on + strand, within Pf1N1B4_1132at 1270.967 kb on + strand, within Pf1N1B4_1132at 1270.998 kb on - strand, within Pf1N1B4_1132at 1271.396 kb on + strand, within Pf1N1B4_1133at 1271.397 kb on - strand, within Pf1N1B4_1133at 1271.483 kb on + strand, within Pf1N1B4_1133at 1271.483 kb on + strandat 1271.483 kb on + strand, within Pf1N1B4_1133at 1271.483 kb on + strand, within Pf1N1B4_1133at 1271.483 kb on + strand, within Pf1N1B4_1133at 1271.484 kb on - strand, within Pf1N1B4_1133at 1271.484 kb on - strand, within Pf1N1B4_1133at 1271.484 kb on - strand, within Pf1N1B4_1133at 1271.484 kb on - strand, within Pf1N1B4_1133at 1271.484 kb on - strand, within Pf1N1B4_1133at 1271.484 kb on - strand, within Pf1N1B4_1133at 1271.679 kb on + strand, within Pf1N1B4_1133at 1271.679 kb on + strand, within Pf1N1B4_1133at 1271.680 kb on - strand, within Pf1N1B4_1133at 1271.680 kb on - strand, within Pf1N1B4_1133at 1271.680 kb on - strand, within Pf1N1B4_1133at 1272.054 kb on + strandat 1272.054 kb on + strand, within Pf1N1B4_1134at 1272.055 kb on - strand, within Pf1N1B4_1134at 1272.055 kb on - strand, within Pf1N1B4_1134at 1272.064 kb on + strand, within Pf1N1B4_1134at 1272.065 kb on - strand, within Pf1N1B4_1134at 1272.065 kb on - strand, within Pf1N1B4_1134at 1272.211 kb on + strand, within Pf1N1B4_1134at 1272.556 kb on - strand, within Pf1N1B4_1134at 1272.667 kb on + strand, within Pf1N1B4_1134at 1272.706 kb on + strand, within Pf1N1B4_1134at 1272.754 kb on + strand, within Pf1N1B4_1134at 1272.755 kb on - strand, within Pf1N1B4_1134at 1272.755 kb on - strand, within Pf1N1B4_1134at 1272.784 kb on + strand, within Pf1N1B4_1134at 1272.784 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.797 kb on + strand, within Pf1N1B4_1134at 1272.798 kb on - strand, within Pf1N1B4_1134at 1272.798 kb on - strand, within Pf1N1B4_1134at 1272.798 kb on - strand, within Pf1N1B4_1134at 1272.798 kb on - strand, within Pf1N1B4_1134at 1272.798 kb on - strand, within Pf1N1B4_1134at 1272.798 kb on - strand, within Pf1N1B4_1134at 1272.798 kb on - strand, within Pf1N1B4_1134at 1272.801 kb on + strand, within Pf1N1B4_1134at 1272.801 kb on + strand, within Pf1N1B4_1134at 1272.801 kb on + strand, within Pf1N1B4_1134at 1272.801 kb on + strand, within Pf1N1B4_1134at 1272.801 kb on + strand, within Pf1N1B4_1134at 1272.802 kb on - strand, within Pf1N1B4_1134at 1272.820 kb on + strand, within Pf1N1B4_1134at 1272.820 kb on + strand, within Pf1N1B4_1134at 1272.820 kb on + strand, within Pf1N1B4_1134at 1272.820 kb on + strand, within Pf1N1B4_1134at 1272.821 kb on - strand, within Pf1N1B4_1134at 1272.821 kb on - strand, within Pf1N1B4_1134at 1272.904 kb on + strandat 1273.021 kb on - strandat 1273.021 kb on - strandat 1273.036 kb on - strandat 1273.111 kb on - strandat 1273.120 kb on - strandat 1273.151 kb on + strandat 1273.152 kb on - strandat 1273.152 kb on - strandat 1273.152 kb on - strandat 1273.152 kb on - strandat 1273.152 kb on - strandat 1273.152 kb on - strandat 1273.159 kb on + strandat 1273.159 kb on + strandat 1273.159 kb on + strandat 1273.159 kb on + strandat 1273.160 kb on - strandat 1273.160 kb on - strandat 1273.160 kb on - strandat 1273.283 kb on - strand, within Pf1N1B4_1135at 1273.283 kb on - strand, within Pf1N1B4_1135at 1273.283 kb on - strand, within Pf1N1B4_1135at 1273.283 kb on - strand, within Pf1N1B4_1135at 1273.283 kb on - strand, within Pf1N1B4_1135at 1273.362 kb on + strandat 1273.362 kb on + strandat 1273.412 kb on - strandat 1273.464 kb on - strandat 1273.599 kb on - strand, within Pf1N1B4_1136at 1273.599 kb on - strand, within Pf1N1B4_1136at 1273.599 kb on - strand, within Pf1N1B4_1136at 1273.599 kb on - strand, within Pf1N1B4_1136at 1273.601 kb on + strand, within Pf1N1B4_1136at 1273.602 kb on - strand, within Pf1N1B4_1136at 1273.682 kb on + strand, within Pf1N1B4_1136at 1273.683 kb on - strand, within Pf1N1B4_1136at 1273.770 kb on - strand, within Pf1N1B4_1136at 1273.776 kb on - strand, within Pf1N1B4_1136at 1273.796 kb on + strand, within Pf1N1B4_1136at 1273.796 kb on + strand, within Pf1N1B4_1136at 1273.797 kb on - strand, within Pf1N1B4_1136at 1273.859 kb on + strand, within Pf1N1B4_1136at 1273.860 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB
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1,270,854 - Pf1N1B4_1132 0.66 +0.8
1,270,854 - Pf1N1B4_1132 0.66 +0.8
1,270,896 - -0.8
1,270,896 - Pf1N1B4_1132 0.71 +0.9
1,270,896 - Pf1N1B4_1132 0.71 -0.8
1,270,955 + Pf1N1B4_1132 0.78 +0.8
1,270,955 + Pf1N1B4_1132 0.78 +0.8
1,270,955 + Pf1N1B4_1132 0.78 +1.2
1,270,955 + Pf1N1B4_1132 0.78 +0.8
1,270,955 + -3.1
1,270,955 + Pf1N1B4_1132 0.78 -1.2
1,270,955 + Pf1N1B4_1132 0.78 +0.8
1,270,955 + Pf1N1B4_1132 0.78 +0.9
1,270,955 + Pf1N1B4_1132 0.78 +0.8
1,270,956 - Pf1N1B4_1132 0.78 +0.8
1,270,956 - Pf1N1B4_1132 0.78 -1.6
1,270,956 - Pf1N1B4_1132 0.78 +0.8
1,270,956 - Pf1N1B4_1132 0.78 -0.2
1,270,967 + Pf1N1B4_1132 0.79 -1.3
1,270,967 + Pf1N1B4_1132 0.79 +0.3
1,270,967 + Pf1N1B4_1132 0.79 +0.8
1,270,998 - Pf1N1B4_1132 0.82 -2.4
1,271,396 + Pf1N1B4_1133 0.35 +0.1
1,271,397 - Pf1N1B4_1133 0.35 +0.8
1,271,483 + Pf1N1B4_1133 0.48 +0.1
1,271,483 + +0.8
1,271,483 + Pf1N1B4_1133 0.48 -2.2
1,271,483 + Pf1N1B4_1133 0.48 -0.2
1,271,483 + Pf1N1B4_1133 0.48 -1.8
1,271,484 - Pf1N1B4_1133 0.48 -0.3
1,271,484 - Pf1N1B4_1133 0.48 -0.2
1,271,484 - Pf1N1B4_1133 0.48 -0.9
1,271,484 - Pf1N1B4_1133 0.48 -1.2
1,271,484 - Pf1N1B4_1133 0.48 -0.8
1,271,484 - Pf1N1B4_1133 0.48 -1.6
1,271,679 + Pf1N1B4_1133 0.78 -2.4
1,271,679 + Pf1N1B4_1133 0.78 -1.8
1,271,680 - Pf1N1B4_1133 0.78 +2.4
1,271,680 - Pf1N1B4_1133 0.78 -2.0
1,271,680 - Pf1N1B4_1133 0.78 +0.8
1,272,054 + -0.7
1,272,054 + Pf1N1B4_1134 0.20 -0.2
1,272,055 - Pf1N1B4_1134 0.20 +0.8
1,272,055 - Pf1N1B4_1134 0.20 +0.8
1,272,064 + Pf1N1B4_1134 0.21 +1.2
1,272,065 - Pf1N1B4_1134 0.21 -2.8
1,272,065 - Pf1N1B4_1134 0.21 -0.8
1,272,211 + Pf1N1B4_1134 0.34 +0.8
1,272,556 - Pf1N1B4_1134 0.64 -1.5
1,272,667 + Pf1N1B4_1134 0.73 -1.5
1,272,706 + Pf1N1B4_1134 0.77 +0.2
1,272,754 + Pf1N1B4_1134 0.81 -0.6
1,272,755 - Pf1N1B4_1134 0.81 -1.0
1,272,755 - Pf1N1B4_1134 0.81 +0.8
1,272,784 + Pf1N1B4_1134 0.83 +0.6
1,272,784 + Pf1N1B4_1134 0.83 +0.8
1,272,797 + Pf1N1B4_1134 0.85 -0.5
1,272,797 + Pf1N1B4_1134 0.85 -0.2
1,272,797 + Pf1N1B4_1134 0.85 +0.4
1,272,797 + Pf1N1B4_1134 0.85 -4.0
1,272,797 + Pf1N1B4_1134 0.85 +0.8
1,272,797 + Pf1N1B4_1134 0.85 -0.2
1,272,797 + Pf1N1B4_1134 0.85 -0.8
1,272,797 + Pf1N1B4_1134 0.85 +0.8
1,272,797 + Pf1N1B4_1134 0.85 +2.7
1,272,797 + Pf1N1B4_1134 0.85 +0.2
1,272,797 + Pf1N1B4_1134 0.85 -1.1
1,272,798 - Pf1N1B4_1134 0.85 +0.8
1,272,798 - Pf1N1B4_1134 0.85 +0.2
1,272,798 - Pf1N1B4_1134 0.85 -1.1
1,272,798 - Pf1N1B4_1134 0.85 -0.2
1,272,798 - Pf1N1B4_1134 0.85 +1.1
1,272,798 - Pf1N1B4_1134 0.85 +0.8
1,272,798 - Pf1N1B4_1134 0.85 +0.8
1,272,801 + Pf1N1B4_1134 0.85 +0.8
1,272,801 + Pf1N1B4_1134 0.85 -2.1
1,272,801 + Pf1N1B4_1134 0.85 +0.8
1,272,801 + Pf1N1B4_1134 0.85 -0.1
1,272,801 + Pf1N1B4_1134 0.85 +0.8
1,272,802 - Pf1N1B4_1134 0.85 -1.2
1,272,820 + Pf1N1B4_1134 0.87 -3.5
1,272,820 + Pf1N1B4_1134 0.87 +0.8
1,272,820 + Pf1N1B4_1134 0.87 -0.2
1,272,820 + Pf1N1B4_1134 0.87 -0.7
1,272,821 - Pf1N1B4_1134 0.87 -0.9
1,272,821 - Pf1N1B4_1134 0.87 +0.2
1,272,904 + -0.8
1,273,021 - +0.7
1,273,021 - +0.8
1,273,036 - -0.6
1,273,111 - +0.2
1,273,120 - -0.1
1,273,151 + -0.7
1,273,152 - -0.6
1,273,152 - -0.6
1,273,152 - +0.4
1,273,152 - -0.2
1,273,152 - +0.8
1,273,152 - +0.8
1,273,159 + -0.0
1,273,159 + -1.0
1,273,159 + -1.3
1,273,159 + -2.2
1,273,160 - -0.7
1,273,160 - -3.7
1,273,160 - +0.8
1,273,283 - Pf1N1B4_1135 0.84 -0.7
1,273,283 - Pf1N1B4_1135 0.84 -2.5
1,273,283 - Pf1N1B4_1135 0.84 -4.0
1,273,283 - Pf1N1B4_1135 0.84 +0.8
1,273,283 - Pf1N1B4_1135 0.84 -3.3
1,273,362 + +0.8
1,273,362 + -1.8
1,273,412 - -1.2
1,273,464 - -2.3
1,273,599 - Pf1N1B4_1136 0.13 -1.8
1,273,599 - Pf1N1B4_1136 0.13 -2.4
1,273,599 - Pf1N1B4_1136 0.13 -0.0
1,273,599 - Pf1N1B4_1136 0.13 -1.2
1,273,601 + Pf1N1B4_1136 0.14 +0.9
1,273,602 - Pf1N1B4_1136 0.14 +0.8
1,273,682 + Pf1N1B4_1136 0.21 -0.8
1,273,683 - Pf1N1B4_1136 0.21 +0.8
1,273,770 - Pf1N1B4_1136 0.28 +0.8
1,273,776 - Pf1N1B4_1136 0.29 -0.6
1,273,796 + Pf1N1B4_1136 0.30 -3.5
1,273,796 + Pf1N1B4_1136 0.30 -0.1
1,273,797 - Pf1N1B4_1136 0.30 +0.8
1,273,859 + Pf1N1B4_1136 0.36 -2.0
1,273,860 - -2.2

Or see this region's nucleotide sequence