Experiment: N2E2wt with N1B4m denitrifying repB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_99 and Pf1N1B4_100 are separated by 308 nucleotides Pf1N1B4_100 and Pf1N1B4_101 are separated by 64 nucleotides Pf1N1B4_101 and Pf1N1B4_102 are separated by 374 nucleotides
Pf1N1B4_99 - Purine nucleoside permease, at 104,512 to 105,546
_99
Pf1N1B4_100 - FIG00953437: hypothetical protein, at 105,855 to 106,082
_100
Pf1N1B4_101 - hypothetical protein, at 106,147 to 106,311
_101
Pf1N1B4_102 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 106,686 to 108,446
_102
Position (kb) 105
106
107 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 104.902 kb on + strand, within Pf1N1B4_99 at 104.902 kb on + strand, within Pf1N1B4_99 at 104.903 kb on - strand, within Pf1N1B4_99 at 104.903 kb on - strand at 105.020 kb on - strand, within Pf1N1B4_99 at 105.192 kb on + strand, within Pf1N1B4_99 at 105.192 kb on + strand, within Pf1N1B4_99 at 105.255 kb on + strand, within Pf1N1B4_99 at 105.268 kb on + strand, within Pf1N1B4_99 at 105.268 kb on + strand, within Pf1N1B4_99 at 105.269 kb on - strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.302 kb on - strand at 105.302 kb on - strand, within Pf1N1B4_99 at 105.428 kb on - strand at 105.657 kb on + strand at 105.657 kb on + strand at 105.657 kb on + strand at 105.658 kb on - strand at 105.791 kb on - strand at 105.828 kb on - strand at 105.828 kb on - strand at 105.829 kb on + strand at 105.830 kb on - strand at 105.830 kb on - strand at 105.833 kb on + strand at 105.833 kb on + strand at 105.833 kb on + strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.834 kb on - strand at 105.845 kb on + strand at 105.846 kb on - strand at 105.854 kb on + strand at 105.885 kb on + strand, within Pf1N1B4_100 at 105.885 kb on + strand, within Pf1N1B4_100 at 105.885 kb on + strand, within Pf1N1B4_100 at 105.886 kb on - strand, within Pf1N1B4_100 at 106.226 kb on + strand, within Pf1N1B4_101 at 106.226 kb on + strand, within Pf1N1B4_101 at 106.227 kb on - strand, within Pf1N1B4_101 at 106.293 kb on + strand, within Pf1N1B4_101 at 106.424 kb on + strand at 106.424 kb on + strand at 106.424 kb on + strand at 106.424 kb on + strand at 106.428 kb on - strand at 106.428 kb on - strand at 106.501 kb on + strand at 106.502 kb on - strand at 106.549 kb on + strand at 106.550 kb on - strand at 106.677 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repB remove 104,902 + Pf1N1B4_99 0.38 -0.7 104,902 + Pf1N1B4_99 0.38 +0.0 104,903 - Pf1N1B4_99 0.38 -0.3 104,903 - -0.4 105,020 - Pf1N1B4_99 0.49 -0.4 105,192 + Pf1N1B4_99 0.66 +0.8 105,192 + Pf1N1B4_99 0.66 -0.4 105,255 + Pf1N1B4_99 0.72 +0.4 105,268 + Pf1N1B4_99 0.73 +0.9 105,268 + Pf1N1B4_99 0.73 +0.7 105,269 - Pf1N1B4_99 0.73 +0.2 105,301 + Pf1N1B4_99 0.76 -1.2 105,301 + Pf1N1B4_99 0.76 -0.2 105,301 + Pf1N1B4_99 0.76 -1.3 105,301 + Pf1N1B4_99 0.76 +0.7 105,301 + Pf1N1B4_99 0.76 +1.3 105,302 - +0.3 105,302 - Pf1N1B4_99 0.76 +0.4 105,428 - +0.7 105,657 + -0.4 105,657 + -1.3 105,657 + -0.7 105,658 - -3.4 105,791 - +0.5 105,828 - -0.3 105,828 - -0.5 105,829 + -0.9 105,830 - -2.8 105,830 - +0.8 105,833 + +0.7 105,833 + +0.7 105,833 + -2.6 105,834 - -3.0 105,834 - -1.6 105,834 - -0.6 105,834 - -1.9 105,834 - +0.5 105,834 - -0.2 105,834 - -4.4 105,834 - +0.3 105,845 + -0.6 105,846 - -0.5 105,854 + +0.0 105,885 + Pf1N1B4_100 0.13 -1.0 105,885 + Pf1N1B4_100 0.13 -2.5 105,885 + Pf1N1B4_100 0.13 -2.6 105,886 - Pf1N1B4_100 0.14 +0.7 106,226 + Pf1N1B4_101 0.48 +0.9 106,226 + Pf1N1B4_101 0.48 +0.6 106,227 - Pf1N1B4_101 0.48 -1.3 106,293 + Pf1N1B4_101 0.88 +3.3 106,424 + -0.9 106,424 + -2.5 106,424 + -0.6 106,424 + +0.1 106,428 - -0.3 106,428 - -1.2 106,501 + -1.4 106,502 - +0.7 106,549 + +0.7 106,550 - -0.9 106,677 + -1.8
Or see this region's nucleotide sequence