Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5763 and Pf1N1B4_5764 are separated by 199 nucleotides Pf1N1B4_5764 and Pf1N1B4_5765 are separated by 24 nucleotides Pf1N1B4_5765 and Pf1N1B4_5766 are separated by 16 nucleotides Pf1N1B4_5766 and Pf1N1B4_5767 overlap by 4 nucleotides
Pf1N1B4_5763 - Nitric oxide reductase activation protein NorQ, at 202,142 to 202,933
_5763
Pf1N1B4_5764 - Cytochrome cd1 nitrite reductase (EC:1.7.2.1), at 203,133 to 204,824
_5764
Pf1N1B4_5765 - membrane c-type cytochrome cy, at 204,849 to 205,163
_5765
Pf1N1B4_5766 - Cytochrome c55X precursor NirC, at 205,180 to 205,506
_5766
Pf1N1B4_5767 - Heme d1 biosynthesis protein NirF, at 205,503 to 206,681
_5767
Position (kb) 203
204
205 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 202.270 kb on + strand, within Pf1N1B4_5763 at 202.501 kb on - strand, within Pf1N1B4_5763 at 202.764 kb on + strand, within Pf1N1B4_5763 at 202.902 kb on + strand at 202.902 kb on + strand at 202.903 kb on - strand at 202.903 kb on - strand at 203.053 kb on - strand at 203.053 kb on - strand at 203.069 kb on - strand at 203.436 kb on + strand, within Pf1N1B4_5764 at 203.437 kb on - strand, within Pf1N1B4_5764 at 203.437 kb on - strand, within Pf1N1B4_5764 at 203.908 kb on - strand, within Pf1N1B4_5764 at 203.908 kb on - strand, within Pf1N1B4_5764 at 203.908 kb on - strand, within Pf1N1B4_5764 at 204.019 kb on - strand, within Pf1N1B4_5764 at 204.148 kb on - strand at 204.148 kb on - strand, within Pf1N1B4_5764 at 204.148 kb on - strand, within Pf1N1B4_5764 at 204.148 kb on - strand, within Pf1N1B4_5764 at 204.148 kb on - strand, within Pf1N1B4_5764 at 204.148 kb on - strand, within Pf1N1B4_5764 at 204.334 kb on - strand, within Pf1N1B4_5764 at 204.334 kb on - strand, within Pf1N1B4_5764 at 204.498 kb on + strand, within Pf1N1B4_5764 at 204.499 kb on - strand, within Pf1N1B4_5764 at 204.648 kb on + strand, within Pf1N1B4_5764 at 204.648 kb on + strand, within Pf1N1B4_5764 at 204.648 kb on + strand, within Pf1N1B4_5764 at 204.754 kb on + strand at 204.755 kb on - strand at 204.819 kb on + strand at 204.819 kb on + strand at 204.819 kb on + strand at 204.819 kb on + strand at 204.820 kb on - strand at 204.820 kb on - strand at 204.820 kb on - strand at 204.820 kb on - strand at 204.857 kb on + strand at 205.201 kb on - strand at 205.526 kb on - strand at 205.725 kb on + strand, within Pf1N1B4_5767 at 205.725 kb on + strand, within Pf1N1B4_5767 at 205.725 kb on + strand, within Pf1N1B4_5767 at 205.725 kb on + strand at 205.726 kb on - strand, within Pf1N1B4_5767 at 205.726 kb on - strand, within Pf1N1B4_5767 at 205.726 kb on - strand, within Pf1N1B4_5767 at 205.726 kb on - strand, within Pf1N1B4_5767 at 205.726 kb on - strand, within Pf1N1B4_5767 at 205.726 kb on - strand, within Pf1N1B4_5767 at 205.796 kb on - strand, within Pf1N1B4_5767
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 202,270 + Pf1N1B4_5763 0.16 +0.6 202,501 - Pf1N1B4_5763 0.45 -1.4 202,764 + Pf1N1B4_5763 0.79 -1.3 202,902 + +0.4 202,902 + -1.0 202,903 - -2.4 202,903 - +0.6 203,053 - -1.2 203,053 - +0.6 203,069 - -0.7 203,436 + Pf1N1B4_5764 0.18 -1.1 203,437 - Pf1N1B4_5764 0.18 -1.0 203,437 - Pf1N1B4_5764 0.18 -0.3 203,908 - Pf1N1B4_5764 0.46 +0.6 203,908 - Pf1N1B4_5764 0.46 +1.1 203,908 - Pf1N1B4_5764 0.46 -3.8 204,019 - Pf1N1B4_5764 0.52 -1.6 204,148 - +0.6 204,148 - Pf1N1B4_5764 0.60 -1.0 204,148 - Pf1N1B4_5764 0.60 -1.3 204,148 - Pf1N1B4_5764 0.60 -0.1 204,148 - Pf1N1B4_5764 0.60 +0.6 204,148 - Pf1N1B4_5764 0.60 -0.8 204,334 - Pf1N1B4_5764 0.71 -1.0 204,334 - Pf1N1B4_5764 0.71 -0.7 204,498 + Pf1N1B4_5764 0.81 -1.2 204,499 - Pf1N1B4_5764 0.81 -1.1 204,648 + Pf1N1B4_5764 0.90 -3.3 204,648 + Pf1N1B4_5764 0.90 +0.6 204,648 + Pf1N1B4_5764 0.90 -1.0 204,754 + -0.9 204,755 - -1.3 204,819 + +0.6 204,819 + -0.8 204,819 + -0.2 204,819 + +0.6 204,820 - -2.3 204,820 - -1.8 204,820 - -0.9 204,820 - +0.6 204,857 + -0.1 205,201 - -0.2 205,526 - +0.6 205,725 + Pf1N1B4_5767 0.19 -1.6 205,725 + Pf1N1B4_5767 0.19 +0.6 205,725 + Pf1N1B4_5767 0.19 -1.4 205,725 + +1.6 205,726 - Pf1N1B4_5767 0.19 +0.6 205,726 - Pf1N1B4_5767 0.19 +0.6 205,726 - Pf1N1B4_5767 0.19 +0.6 205,726 - Pf1N1B4_5767 0.19 -0.4 205,726 - Pf1N1B4_5767 0.19 +0.6 205,726 - Pf1N1B4_5767 0.19 -1.4 205,796 - Pf1N1B4_5767 0.25 -0.4
Or see this region's nucleotide sequence