Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5746 and Pf1N1B4_5747 are separated by 132 nucleotides Pf1N1B4_5747 and Pf1N1B4_5748 are separated by 30 nucleotides Pf1N1B4_5748 and Pf1N1B4_5749 are separated by 262 nucleotides
Pf1N1B4_5746 - FIG139552: Putative protease, at 187,631 to 188,626
_5746
Pf1N1B4_5747 - Molybdopterin biosynthesis protein MoeA, at 188,759 to 189,538
_5747
Pf1N1B4_5748 - hypothetical protein, at 189,569 to 189,727
_5748
Pf1N1B4_5749 - Molybdenum cofactor biosynthesis protein MoaB, at 189,990 to 190,544
_5749
Position (kb) 188
189
190 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 187.788 kb on + strand, within Pf1N1B4_5746 at 187.788 kb on + strand at 188.085 kb on + strand, within Pf1N1B4_5746 at 188.086 kb on - strand at 188.123 kb on + strand, within Pf1N1B4_5746 at 188.123 kb on + strand, within Pf1N1B4_5746 at 188.124 kb on - strand, within Pf1N1B4_5746 at 188.124 kb on - strand at 188.180 kb on + strand, within Pf1N1B4_5746 at 188.181 kb on - strand, within Pf1N1B4_5746 at 188.241 kb on + strand, within Pf1N1B4_5746 at 188.242 kb on - strand, within Pf1N1B4_5746 at 188.322 kb on + strand, within Pf1N1B4_5746 at 188.322 kb on + strand, within Pf1N1B4_5746 at 188.322 kb on + strand, within Pf1N1B4_5746 at 188.553 kb on + strand at 188.554 kb on - strand at 188.944 kb on - strand, within Pf1N1B4_5747 at 189.009 kb on - strand, within Pf1N1B4_5747 at 189.316 kb on - strand, within Pf1N1B4_5747 at 189.378 kb on + strand at 189.378 kb on + strand, within Pf1N1B4_5747 at 189.378 kb on + strand, within Pf1N1B4_5747 at 189.379 kb on - strand, within Pf1N1B4_5747 at 189.593 kb on - strand, within Pf1N1B4_5748 at 190.158 kb on - strand, within Pf1N1B4_5749 at 190.379 kb on - strand, within Pf1N1B4_5749 at 190.415 kb on + strand, within Pf1N1B4_5749 at 190.415 kb on + strand, within Pf1N1B4_5749 at 190.415 kb on + strand, within Pf1N1B4_5749 at 190.416 kb on - strand, within Pf1N1B4_5749 at 190.416 kb on - strand, within Pf1N1B4_5749 at 190.416 kb on - strand, within Pf1N1B4_5749
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 187,788 + Pf1N1B4_5746 0.16 -2.0 187,788 + +0.6 188,085 + Pf1N1B4_5746 0.46 +0.6 188,086 - -1.4 188,123 + Pf1N1B4_5746 0.49 +1.6 188,123 + Pf1N1B4_5746 0.49 +0.6 188,124 - Pf1N1B4_5746 0.49 +0.6 188,124 - -0.0 188,180 + Pf1N1B4_5746 0.55 -0.2 188,181 - Pf1N1B4_5746 0.55 +0.6 188,241 + Pf1N1B4_5746 0.61 +0.6 188,242 - Pf1N1B4_5746 0.61 -1.5 188,322 + Pf1N1B4_5746 0.69 +0.6 188,322 + Pf1N1B4_5746 0.69 +0.3 188,322 + Pf1N1B4_5746 0.69 -0.1 188,553 + -0.4 188,554 - -0.5 188,944 - Pf1N1B4_5747 0.24 +0.6 189,009 - Pf1N1B4_5747 0.32 -1.8 189,316 - Pf1N1B4_5747 0.71 -1.8 189,378 + +0.6 189,378 + Pf1N1B4_5747 0.79 -1.3 189,378 + Pf1N1B4_5747 0.79 -0.2 189,379 - Pf1N1B4_5747 0.79 +0.6 189,593 - Pf1N1B4_5748 0.15 -0.8 190,158 - Pf1N1B4_5749 0.30 -0.0 190,379 - Pf1N1B4_5749 0.70 -3.1 190,415 + Pf1N1B4_5749 0.77 -1.0 190,415 + Pf1N1B4_5749 0.77 -1.3 190,415 + Pf1N1B4_5749 0.77 +0.6 190,416 - Pf1N1B4_5749 0.77 -1.4 190,416 - Pf1N1B4_5749 0.77 -0.3 190,416 - Pf1N1B4_5749 0.77 -1.2
Or see this region's nucleotide sequence