Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5730 and Pf1N1B4_5731 are separated by 25 nucleotides Pf1N1B4_5731 and Pf1N1B4_5732 are separated by 10 nucleotides Pf1N1B4_5732 and Pf1N1B4_5733 overlap by 8 nucleotides Pf1N1B4_5733 and Pf1N1B4_5734 are separated by 2 nucleotides
Pf1N1B4_5730 - Molybdenum cofactor biosynthesis protein MoaA, at 169,868 to 170,860
_5730
Pf1N1B4_5731 - Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8), at 170,886 to 171,845
_5731
Pf1N1B4_5732 - Respiratory nitrate reductase gamma chain (EC 1.7.99.4), at 171,856 to 172,536
_5732
Pf1N1B4_5733 - Respiratory nitrate reductase delta chain (EC 1.7.99.4), at 172,529 to 173,284
_5733
Pf1N1B4_5734 - Respiratory nitrate reductase beta chain (EC 1.7.99.4), at 173,287 to 174,825
_5734
Position (kb) 171
172
173 Strain fitness (log2 ratio) -2
-1
0
1 at 171.218 kb on - strand, within Pf1N1B4_5731 at 171.394 kb on + strand, within Pf1N1B4_5731 at 171.860 kb on + strand at 171.860 kb on + strand at 171.861 kb on - strand at 171.897 kb on - strand at 171.984 kb on - strand, within Pf1N1B4_5732 at 172.502 kb on + strand at 173.474 kb on + strand, within Pf1N1B4_5734 at 173.475 kb on - strand, within Pf1N1B4_5734 at 173.475 kb on - strand, within Pf1N1B4_5734 at 173.499 kb on - strand, within Pf1N1B4_5734 at 173.499 kb on - strand at 173.499 kb on - strand, within Pf1N1B4_5734
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 171,218 - Pf1N1B4_5731 0.35 +0.6 171,394 + Pf1N1B4_5731 0.53 -1.8 171,860 + -1.4 171,860 + -1.4 171,861 - +0.6 171,897 - -2.7 171,984 - Pf1N1B4_5732 0.19 -0.6 172,502 + -2.4 173,474 + Pf1N1B4_5734 0.12 +0.6 173,475 - Pf1N1B4_5734 0.12 +0.6 173,475 - Pf1N1B4_5734 0.12 -1.8 173,499 - Pf1N1B4_5734 0.14 +0.6 173,499 - +0.6 173,499 - Pf1N1B4_5734 0.14 +0.3
Or see this region's nucleotide sequence