Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5098

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5096 and Pf1N1B4_5097 are separated by 135 nucleotidesPf1N1B4_5097 and Pf1N1B4_5098 are separated by 64 nucleotidesPf1N1B4_5098 and Pf1N1B4_5099 are separated by 145 nucleotides Pf1N1B4_5096 - Nitrate ABC transporter, nitrate-binding protein, at 5,649,738 to 5,651,024 _5096 Pf1N1B4_5097 - Cyanate ABC transporter, permease protein, at 5,651,160 to 5,651,891 _5097 Pf1N1B4_5098 - Nitrate ABC transporter, ATP-binding protein, at 5,651,956 to 5,652,747 _5098 Pf1N1B4_5099 - Proline iminopeptidase (EC 3.4.11.5), at 5,652,893 to 5,653,837 _5099 Position (kb) 5651 5652 5653Strain fitness (log2 ratio) -1 0 1at 5650.995 kb on + strandat 5651.195 kb on + strandat 5651.441 kb on - strandat 5651.441 kb on - strand, within Pf1N1B4_5097at 5653.207 kb on - strand, within Pf1N1B4_5099at 5653.241 kb on - strand, within Pf1N1B4_5099at 5653.528 kb on - strand, within Pf1N1B4_5099at 5653.689 kb on + strand, within Pf1N1B4_5099at 5653.690 kb on - strandat 5653.690 kb on - strand, within Pf1N1B4_5099at 5653.690 kb on - strand, within Pf1N1B4_5099at 5653.690 kb on - strand, within Pf1N1B4_5099at 5653.702 kb on - strand, within Pf1N1B4_5099

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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5,650,995 + -1.6
5,651,195 + +0.4
5,651,441 - +0.1
5,651,441 - Pf1N1B4_5097 0.38 -0.6
5,653,207 - Pf1N1B4_5099 0.33 -1.2
5,653,241 - Pf1N1B4_5099 0.37 +0.2
5,653,528 - Pf1N1B4_5099 0.67 -0.3
5,653,689 + Pf1N1B4_5099 0.84 -1.3
5,653,690 - -0.6
5,653,690 - Pf1N1B4_5099 0.84 +0.6
5,653,690 - Pf1N1B4_5099 0.84 -1.7
5,653,690 - Pf1N1B4_5099 0.84 -0.2
5,653,702 - Pf1N1B4_5099 0.86 +0.0

Or see this region's nucleotide sequence