Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4976 and Pf1N1B4_4977 are separated by 111 nucleotides Pf1N1B4_4977 and Pf1N1B4_4978 are separated by 51 nucleotides
Pf1N1B4_4976 - D-serine/D-alanine/glycine transporter, at 5,515,291 to 5,516,712
_4976
Pf1N1B4_4977 - Ornithine cyclodeaminase (EC 4.3.1.12), at 5,516,824 to 5,517,849
_4977
Pf1N1B4_4978 - Deacetylases, including yeast histone deacetylase and acetoin utilization protein, at 5,517,901 to 5,518,935
_4978
Position (kb) 5516
5517
5518 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5516.038 kb on + strand, within Pf1N1B4_4976 at 5516.409 kb on - strand, within Pf1N1B4_4976 at 5516.492 kb on - strand, within Pf1N1B4_4976 at 5516.547 kb on + strand at 5516.549 kb on + strand, within Pf1N1B4_4976 at 5516.554 kb on - strand, within Pf1N1B4_4976 at 5516.557 kb on + strand, within Pf1N1B4_4976 at 5517.038 kb on + strand, within Pf1N1B4_4977 at 5517.038 kb on + strand, within Pf1N1B4_4977 at 5517.186 kb on + strand, within Pf1N1B4_4977 at 5517.320 kb on + strand at 5517.321 kb on - strand, within Pf1N1B4_4977 at 5517.641 kb on + strand at 5517.641 kb on + strand, within Pf1N1B4_4977 at 5517.642 kb on - strand, within Pf1N1B4_4977 at 5517.654 kb on - strand at 5517.727 kb on - strand, within Pf1N1B4_4977 at 5517.968 kb on + strand at 5518.102 kb on - strand, within Pf1N1B4_4978 at 5518.106 kb on - strand, within Pf1N1B4_4978 at 5518.163 kb on + strand, within Pf1N1B4_4978 at 5518.208 kb on + strand, within Pf1N1B4_4978 at 5518.253 kb on + strand at 5518.256 kb on + strand, within Pf1N1B4_4978 at 5518.316 kb on + strand, within Pf1N1B4_4978 at 5518.316 kb on + strand, within Pf1N1B4_4978 at 5518.377 kb on + strand, within Pf1N1B4_4978 at 5518.427 kb on + strand at 5518.445 kb on + strand, within Pf1N1B4_4978 at 5518.445 kb on + strand, within Pf1N1B4_4978 at 5518.445 kb on + strand, within Pf1N1B4_4978 at 5518.445 kb on + strand, within Pf1N1B4_4978 at 5518.739 kb on + strand, within Pf1N1B4_4978
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 5,516,038 + Pf1N1B4_4976 0.53 +0.4 5,516,409 - Pf1N1B4_4976 0.79 -1.2 5,516,492 - Pf1N1B4_4976 0.84 -0.9 5,516,547 + -1.2 5,516,549 + Pf1N1B4_4976 0.88 -1.5 5,516,554 - Pf1N1B4_4976 0.89 -0.7 5,516,557 + Pf1N1B4_4976 0.89 -0.4 5,517,038 + Pf1N1B4_4977 0.21 -2.0 5,517,038 + Pf1N1B4_4977 0.21 -0.5 5,517,186 + Pf1N1B4_4977 0.35 -1.4 5,517,320 + +0.4 5,517,321 - Pf1N1B4_4977 0.48 -3.7 5,517,641 + -2.0 5,517,641 + Pf1N1B4_4977 0.80 -1.0 5,517,642 - Pf1N1B4_4977 0.80 -0.6 5,517,654 - +0.4 5,517,727 - Pf1N1B4_4977 0.88 -2.5 5,517,968 + -0.3 5,518,102 - Pf1N1B4_4978 0.19 -0.8 5,518,106 - Pf1N1B4_4978 0.20 -1.5 5,518,163 + Pf1N1B4_4978 0.25 -2.2 5,518,208 + Pf1N1B4_4978 0.30 +0.2 5,518,253 + -3.3 5,518,256 + Pf1N1B4_4978 0.34 -0.5 5,518,316 + Pf1N1B4_4978 0.40 -0.4 5,518,316 + Pf1N1B4_4978 0.40 -0.2 5,518,377 + Pf1N1B4_4978 0.46 -0.9 5,518,427 + +0.4 5,518,445 + Pf1N1B4_4978 0.53 +0.4 5,518,445 + Pf1N1B4_4978 0.53 -0.7 5,518,445 + Pf1N1B4_4978 0.53 -0.4 5,518,445 + Pf1N1B4_4978 0.53 -1.6 5,518,739 + Pf1N1B4_4978 0.81 -0.6
Or see this region's nucleotide sequence