Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4718 and Pf1N1B4_4719 are separated by 52 nucleotides Pf1N1B4_4719 and Pf1N1B4_4720 are separated by 146 nucleotides Pf1N1B4_4720 and Pf1N1B4_4721 are separated by 18 nucleotides Pf1N1B4_4721 and Pf1N1B4_4722 are separated by 116 nucleotides
Pf1N1B4_4718 - Transcriptional regulator, DeoR family, at 5,215,004 to 5,215,720
_4718
Pf1N1B4_4719 - Electron transfer flavoprotein, beta subunit, at 5,215,773 to 5,216,114
_4719
Pf1N1B4_4720 - AFG1-like ATPase, at 5,216,261 to 5,217,379
_4720
Pf1N1B4_4721 - Nuclease inhibitor, at 5,217,398 to 5,217,979
_4721
Pf1N1B4_4722 - Histone acetyltransferase HPA2 and related acetyltransferases, at 5,218,096 to 5,218,587
_4722
Position (kb) 5216
5217
5218 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5216.058 kb on + strand, within Pf1N1B4_4719 at 5216.059 kb on - strand, within Pf1N1B4_4719 at 5216.059 kb on - strand, within Pf1N1B4_4719 at 5216.245 kb on - strand at 5216.248 kb on + strand at 5216.248 kb on + strand at 5216.248 kb on + strand at 5216.248 kb on + strand at 5216.249 kb on - strand at 5216.249 kb on - strand at 5216.409 kb on - strand, within Pf1N1B4_4720 at 5216.409 kb on - strand, within Pf1N1B4_4720 at 5216.409 kb on - strand at 5216.725 kb on - strand, within Pf1N1B4_4720 at 5217.261 kb on - strand, within Pf1N1B4_4720 at 5217.294 kb on - strand at 5217.306 kb on - strand at 5217.576 kb on - strand at 5217.576 kb on - strand, within Pf1N1B4_4721 at 5217.576 kb on - strand, within Pf1N1B4_4721 at 5217.703 kb on + strand, within Pf1N1B4_4721 at 5218.132 kb on + strand at 5218.169 kb on + strand at 5218.224 kb on + strand, within Pf1N1B4_4722
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 5,216,058 + Pf1N1B4_4719 0.83 +1.9 5,216,059 - Pf1N1B4_4719 0.84 +0.1 5,216,059 - Pf1N1B4_4719 0.84 -1.5 5,216,245 - +0.4 5,216,248 + -0.6 5,216,248 + -0.5 5,216,248 + -0.6 5,216,248 + +0.2 5,216,249 - -2.8 5,216,249 - -2.6 5,216,409 - Pf1N1B4_4720 0.13 -1.6 5,216,409 - Pf1N1B4_4720 0.13 -0.9 5,216,409 - +0.4 5,216,725 - Pf1N1B4_4720 0.41 -2.2 5,217,261 - Pf1N1B4_4720 0.89 -0.3 5,217,294 - -1.5 5,217,306 - -1.4 5,217,576 - -1.0 5,217,576 - Pf1N1B4_4721 0.31 -1.1 5,217,576 - Pf1N1B4_4721 0.31 -2.8 5,217,703 + Pf1N1B4_4721 0.52 -0.8 5,218,132 + +0.0 5,218,169 + +0.4 5,218,224 + Pf1N1B4_4722 0.26 +0.5
Or see this region's nucleotide sequence