Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4717 and Pf1N1B4_4718 are separated by 207 nucleotides Pf1N1B4_4718 and Pf1N1B4_4719 are separated by 52 nucleotides Pf1N1B4_4719 and Pf1N1B4_4720 are separated by 146 nucleotides
Pf1N1B4_4717 - Agmatinase (EC 3.5.3.11), at 5,213,837 to 5,214,796
_4717
Pf1N1B4_4718 - Transcriptional regulator, DeoR family, at 5,215,004 to 5,215,720
_4718
Pf1N1B4_4719 - Electron transfer flavoprotein, beta subunit, at 5,215,773 to 5,216,114
_4719
Pf1N1B4_4720 - AFG1-like ATPase, at 5,216,261 to 5,217,379
_4720
Position (kb) 5215
5216
5217 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5214.867 kb on - strand at 5214.867 kb on - strand at 5214.967 kb on - strand at 5215.036 kb on - strand at 5215.036 kb on - strand at 5215.036 kb on - strand at 5215.179 kb on - strand at 5215.179 kb on - strand, within Pf1N1B4_4718 at 5215.179 kb on - strand, within Pf1N1B4_4718 at 5216.058 kb on + strand, within Pf1N1B4_4719 at 5216.059 kb on - strand, within Pf1N1B4_4719 at 5216.059 kb on - strand, within Pf1N1B4_4719 at 5216.245 kb on - strand at 5216.248 kb on + strand at 5216.248 kb on + strand at 5216.248 kb on + strand at 5216.248 kb on + strand at 5216.249 kb on - strand at 5216.249 kb on - strand at 5216.409 kb on - strand, within Pf1N1B4_4720 at 5216.409 kb on - strand, within Pf1N1B4_4720 at 5216.409 kb on - strand at 5216.725 kb on - strand, within Pf1N1B4_4720
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 5,214,867 - -0.6 5,214,867 - -0.8 5,214,967 - +0.0 5,215,036 - -0.9 5,215,036 - +0.4 5,215,036 - +0.4 5,215,179 - -0.2 5,215,179 - Pf1N1B4_4718 0.24 -0.5 5,215,179 - Pf1N1B4_4718 0.24 -0.9 5,216,058 + Pf1N1B4_4719 0.83 +1.9 5,216,059 - Pf1N1B4_4719 0.84 +0.1 5,216,059 - Pf1N1B4_4719 0.84 -1.5 5,216,245 - +0.4 5,216,248 + -0.6 5,216,248 + -0.5 5,216,248 + -0.6 5,216,248 + +0.2 5,216,249 - -2.8 5,216,249 - -2.6 5,216,409 - Pf1N1B4_4720 0.13 -1.6 5,216,409 - Pf1N1B4_4720 0.13 -0.9 5,216,409 - +0.4 5,216,725 - Pf1N1B4_4720 0.41 -2.2
Or see this region's nucleotide sequence