Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_114

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_113 and Pf1N1B4_114 are separated by 112 nucleotidesPf1N1B4_114 and Pf1N1B4_115 are separated by 169 nucleotides Pf1N1B4_113 - Cytosine deaminase (EC 3.5.4.1), at 117,512 to 118,756 _113 Pf1N1B4_114 - Diaminopimelate epimerase (EC 5.1.1.7), at 118,869 to 119,858 _114 Pf1N1B4_115 - Membrane protein mosC, at 120,028 to 121,179 _115 Position (kb) 118 119 120Strain fitness (log2 ratio) -2 -1 0 1at 118.059 kb on - strand, within Pf1N1B4_113at 118.653 kb on - strandat 118.864 kb on - strandat 118.864 kb on - strandat 119.164 kb on + strand, within Pf1N1B4_114at 119.164 kb on + strand, within Pf1N1B4_114at 119.165 kb on - strand, within Pf1N1B4_114at 119.165 kb on - strand, within Pf1N1B4_114at 119.165 kb on - strand, within Pf1N1B4_114at 119.693 kb on + strand, within Pf1N1B4_114at 119.694 kb on - strand, within Pf1N1B4_114at 119.824 kb on + strandat 119.825 kb on - strandat 119.825 kb on - strandat 119.825 kb on - strandat 119.828 kb on + strandat 119.829 kb on - strandat 119.829 kb on - strandat 119.829 kb on - strandat 119.829 kb on - strandat 119.845 kb on + strandat 119.846 kb on - strandat 119.846 kb on - strandat 119.872 kb on - strandat 119.872 kb on - strandat 119.872 kb on - strandat 120.027 kb on + strandat 120.145 kb on + strand, within Pf1N1B4_115at 120.330 kb on + strand, within Pf1N1B4_115at 120.843 kb on + strand, within Pf1N1B4_115

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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118,059 - Pf1N1B4_113 0.44 +0.6
118,653 - -1.0
118,864 - -0.7
118,864 - +0.0
119,164 + Pf1N1B4_114 0.30 -1.7
119,164 + Pf1N1B4_114 0.30 -0.4
119,165 - Pf1N1B4_114 0.30 +0.6
119,165 - Pf1N1B4_114 0.30 -2.0
119,165 - Pf1N1B4_114 0.30 -1.7
119,693 + Pf1N1B4_114 0.83 +1.6
119,694 - Pf1N1B4_114 0.83 -1.1
119,824 + -0.1
119,825 - -0.8
119,825 - -0.1
119,825 - -1.0
119,828 + +0.6
119,829 - -0.6
119,829 - -0.0
119,829 - -0.8
119,829 - -0.2
119,845 + +0.6
119,846 - -1.4
119,846 - +0.6
119,872 - -0.7
119,872 - -0.2
119,872 - +0.6
120,027 + -0.2
120,145 + Pf1N1B4_115 0.10 +0.6
120,330 + Pf1N1B4_115 0.26 +0.6
120,843 + Pf1N1B4_115 0.71 +0.6

Or see this region's nucleotide sequence