Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1081

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1080 and Pf1N1B4_1081 are separated by 79 nucleotidesPf1N1B4_1081 and Pf1N1B4_1082 are separated by 54 nucleotidesPf1N1B4_1082 and Pf1N1B4_1083 are separated by 2 nucleotides Pf1N1B4_1080 - RNA polymerase sigma-54 factor RpoN, at 1,212,175 to 1,213,644 _1080 Pf1N1B4_1081 - Ribosome hibernation protein YhbH, at 1,213,724 to 1,214,032 _1081 Pf1N1B4_1082 - PTS IIA-like nitrogen-regulatory protein PtsN, at 1,214,087 to 1,214,509 _1082 Pf1N1B4_1083 - Hypothetical ATP-binding protein UPF0042, contains P-loop, at 1,214,512 to 1,215,369 _1083 Position (kb) 1213 1214 1215Strain fitness (log2 ratio) -3 -2 -1 0 1at 1213.696 kb on + strandat 1213.696 kb on + strandat 1213.723 kb on + strandat 1213.723 kb on + strandat 1213.723 kb on + strandat 1213.778 kb on + strandat 1213.778 kb on + strand, within Pf1N1B4_1081at 1213.778 kb on + strand, within Pf1N1B4_1081at 1213.888 kb on + strand, within Pf1N1B4_1081at 1213.888 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strandat 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strandat 1213.934 kb on + strandat 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strand, within Pf1N1B4_1081at 1213.934 kb on + strandat 1213.934 kb on + strand, within Pf1N1B4_1081

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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1,213,696 + +0.7
1,213,696 + -0.2
1,213,723 + +0.7
1,213,723 + -1.3
1,213,723 + -1.7
1,213,778 + -1.3
1,213,778 + Pf1N1B4_1081 0.17 +0.7
1,213,778 + Pf1N1B4_1081 0.17 -0.8
1,213,888 + Pf1N1B4_1081 0.53 +0.3
1,213,888 + Pf1N1B4_1081 0.53 +0.7
1,213,934 + -0.3
1,213,934 + Pf1N1B4_1081 0.68 -2.2
1,213,934 + Pf1N1B4_1081 0.68 -1.0
1,213,934 + Pf1N1B4_1081 0.68 +0.7
1,213,934 + Pf1N1B4_1081 0.68 +0.7
1,213,934 + Pf1N1B4_1081 0.68 +0.7
1,213,934 + Pf1N1B4_1081 0.68 -1.0
1,213,934 + Pf1N1B4_1081 0.68 -1.8
1,213,934 + Pf1N1B4_1081 0.68 -1.0
1,213,934 + Pf1N1B4_1081 0.68 -2.5
1,213,934 + Pf1N1B4_1081 0.68 +0.3
1,213,934 + Pf1N1B4_1081 0.68 +0.7
1,213,934 + Pf1N1B4_1081 0.68 +0.7
1,213,934 + Pf1N1B4_1081 0.68 +0.7
1,213,934 + +0.4
1,213,934 + -1.1
1,213,934 + Pf1N1B4_1081 0.68 -0.1
1,213,934 + Pf1N1B4_1081 0.68 -2.0
1,213,934 + Pf1N1B4_1081 0.68 +0.7
1,213,934 + Pf1N1B4_1081 0.68 -0.9
1,213,934 + Pf1N1B4_1081 0.68 -2.8
1,213,934 + Pf1N1B4_1081 0.68 +0.7
1,213,934 + +0.7
1,213,934 + Pf1N1B4_1081 0.68 -2.5

Or see this region's nucleotide sequence