Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20310

Experiment: Thymidine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20300 and BWI76_RS20305 are separated by 217 nucleotidesBWI76_RS20305 and BWI76_RS20310 are separated by 3 nucleotidesBWI76_RS20310 and BWI76_RS20315 are separated by 56 nucleotidesBWI76_RS20315 and BWI76_RS20320 are separated by 41 nucleotides BWI76_RS20300: BWI76_RS20300 - epimerase, at 4,252,241 to 4,253,134 _RS20300 BWI76_RS20305: BWI76_RS20305 - hypothetical protein, at 4,253,352 to 4,253,606 _RS20305 BWI76_RS20310: BWI76_RS20310 - hypothetical protein, at 4,253,610 to 4,254,176 _RS20310 BWI76_RS20315: BWI76_RS20315 - N-acetyltransferase, at 4,254,233 to 4,254,730 _RS20315 BWI76_RS20320: BWI76_RS20320 - histidine/lysine/arginine/ornithine ABC transporter ATP-binding protein HisP, at 4,254,772 to 4,255,545 _RS20320 Position (kb) 4253 4254 4255Strain fitness (log2 ratio) -2 -1 0 1at 4253.258 kb on + strandat 4253.270 kb on + strandat 4253.270 kb on + strandat 4253.270 kb on + strandat 4253.333 kb on + strandat 4253.641 kb on - strandat 4254.107 kb on + strand, within BWI76_RS20310at 4254.107 kb on - strand, within BWI76_RS20310at 4254.115 kb on - strand, within BWI76_RS20310at 4254.122 kb on + strandat 4254.354 kb on + strand, within BWI76_RS20315at 4254.505 kb on - strand, within BWI76_RS20315at 4254.561 kb on + strand, within BWI76_RS20315at 4254.777 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Thymidine (N)
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4,253,258 + +0.1
4,253,270 + +0.4
4,253,270 + +0.3
4,253,270 + +0.8
4,253,333 + -2.0
4,253,641 - +0.2
4,254,107 + BWI76_RS20310 0.88 +0.6
4,254,107 - BWI76_RS20310 0.88 +0.1
4,254,115 - BWI76_RS20310 0.89 +1.7
4,254,122 + -0.1
4,254,354 + BWI76_RS20315 0.24 +0.1
4,254,505 - BWI76_RS20315 0.55 -0.6
4,254,561 + BWI76_RS20315 0.66 +0.3
4,254,777 - +0.1

Or see this region's nucleotide sequence