Experiment: Succinic Acid; Light intensity 75 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0203 and Rru_A0204 are separated by 249 nucleotides Rru_A0204 and Rru_A0205 are separated by 188 nucleotides Rru_A0205 and Rru_A0206 are separated by 72 nucleotides
Rru_A0203: Rru_A0203 - hypothetical protein (NCBI), at 253,299 to 253,607
_A0203
Rru_A0204: Rru_A0204 - BolA-like protein (NCBI), at 253,857 to 254,201
_A0204
Rru_A0205: Rru_A0205 - Heat shock protein DnaJ-like (NCBI), at 254,390 to 255,001
_A0205
Rru_A0206: Rru_A0206 - Cobalt chelatase, pCobS small subunit (NCBI), at 255,074 to 256,063
_A0206
Position (kb)
254
255
256 Strain fitness (log2 ratio)
-1
0
1
2
3 at 253.518 kb on + strand, within Rru_A0203 at 253.598 kb on + strand at 253.598 kb on + strand at 253.669 kb on - strand at 253.704 kb on - strand at 253.726 kb on + strand at 253.726 kb on + strand at 254.363 kb on + strand at 254.364 kb on - strand at 254.364 kb on - strand at 254.366 kb on + strand at 254.366 kb on + strand at 254.366 kb on + strand at 254.367 kb on - strand at 254.367 kb on - strand at 254.367 kb on - strand at 254.367 kb on - strand at 254.546 kb on + strand, within Rru_A0205 at 254.547 kb on - strand, within Rru_A0205 at 254.547 kb on - strand, within Rru_A0205 at 254.547 kb on - strand, within Rru_A0205 at 254.549 kb on + strand, within Rru_A0205 at 254.549 kb on + strand, within Rru_A0205 at 254.549 kb on + strand, within Rru_A0205 at 254.550 kb on - strand, within Rru_A0205 at 254.550 kb on - strand, within Rru_A0205 at 254.579 kb on + strand, within Rru_A0205 at 254.579 kb on + strand, within Rru_A0205 at 254.580 kb on - strand, within Rru_A0205 at 254.580 kb on - strand, within Rru_A0205 at 254.580 kb on - strand, within Rru_A0205 at 254.580 kb on - strand, within Rru_A0205 at 254.580 kb on - strand, within Rru_A0205 at 254.582 kb on - strand, within Rru_A0205 at 254.582 kb on - strand, within Rru_A0205 at 254.597 kb on + strand, within Rru_A0205 at 254.598 kb on - strand, within Rru_A0205 at 254.598 kb on - strand, within Rru_A0205 at 254.601 kb on - strand, within Rru_A0205 at 254.882 kb on + strand, within Rru_A0205 at 254.883 kb on - strand, within Rru_A0205 at 254.883 kb on - strand, within Rru_A0205 at 254.904 kb on - strand, within Rru_A0205 at 254.904 kb on - strand, within Rru_A0205 at 254.955 kb on + strand at 254.956 kb on - strand at 254.970 kb on - strand at 255.092 kb on + strand at 255.103 kb on + strand at 255.104 kb on - strand at 255.104 kb on - strand at 255.212 kb on + strand, within Rru_A0206 at 255.213 kb on - strand, within Rru_A0206 at 255.287 kb on + strand, within Rru_A0206 at 255.500 kb on + strand, within Rru_A0206 at 255.605 kb on + strand, within Rru_A0206 at 255.605 kb on + strand, within Rru_A0206 at 255.729 kb on - strand, within Rru_A0206 at 255.729 kb on - strand, within Rru_A0206 at 255.999 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 75 uM remove 253,518 + Rru_A0203 0.71 -1.0 253,598 + +0.7 253,598 + +0.0 253,669 - +0.7 253,704 - -0.6 253,726 + +0.0 253,726 + -0.8 254,363 + +1.6 254,364 - +0.0 254,364 - +0.0 254,366 + -0.8 254,366 + +0.0 254,366 + +0.0 254,367 - +2.8 254,367 - +0.0 254,367 - +0.0 254,367 - +0.0 254,546 + Rru_A0205 0.25 -1.5 254,547 - Rru_A0205 0.26 +0.0 254,547 - Rru_A0205 0.26 +1.1 254,547 - Rru_A0205 0.26 +0.8 254,549 + Rru_A0205 0.26 +0.0 254,549 + Rru_A0205 0.26 +0.6 254,549 + Rru_A0205 0.26 +0.2 254,550 - Rru_A0205 0.26 +0.0 254,550 - Rru_A0205 0.26 +1.3 254,579 + Rru_A0205 0.31 +0.0 254,579 + Rru_A0205 0.31 -0.5 254,580 - Rru_A0205 0.31 +1.5 254,580 - Rru_A0205 0.31 +1.5 254,580 - Rru_A0205 0.31 +1.1 254,580 - Rru_A0205 0.31 +1.2 254,580 - Rru_A0205 0.31 +0.8 254,582 - Rru_A0205 0.31 +1.0 254,582 - Rru_A0205 0.31 +0.0 254,597 + Rru_A0205 0.34 +0.0 254,598 - Rru_A0205 0.34 +1.0 254,598 - Rru_A0205 0.34 +0.0 254,601 - Rru_A0205 0.34 +1.0 254,882 + Rru_A0205 0.80 +0.5 254,883 - Rru_A0205 0.81 +0.0 254,883 - Rru_A0205 0.81 +0.0 254,904 - Rru_A0205 0.84 +0.0 254,904 - Rru_A0205 0.84 +2.8 254,955 + +0.0 254,956 - +1.6 254,970 - +0.0 255,092 + +0.0 255,103 + -1.3 255,104 - +0.0 255,104 - +0.0 255,212 + Rru_A0206 0.14 +0.9 255,213 - Rru_A0206 0.14 +0.0 255,287 + Rru_A0206 0.22 +0.0 255,500 + Rru_A0206 0.43 +0.0 255,605 + Rru_A0206 0.54 +0.0 255,605 + Rru_A0206 0.54 +0.0 255,729 - Rru_A0206 0.66 +0.0 255,729 - Rru_A0206 0.66 +0.0 255,999 - -1.0
Or see this region's nucleotide sequence