Experiment: Succinic Acid; Light intensity 75 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0015 and Rru_AR0001 are separated by 163 nucleotides Rru_AR0001 and Rru_A0016 are separated by 253 nucleotides Rru_A0016 and Rru_A0017 are separated by 114 nucleotides
Rru_A0015: Rru_A0015 - 3'-5' exonuclease (NCBI), at 16,232 to 16,852
_A0015
Rru_AR0001: Rru_AR0001 - tRNA-Leu (NCBI), at 17,016 to 17,102
_AR0001
Rru_A0016: Rru_A0016 - 3-beta-hydroxy-delta(5)-steroid dehydrogenase (NCBI), at 17,356 to 18,378
_A0016
Rru_A0017: Rru_A0017 - Bacitracin resistance protein BacA (NCBI), at 18,493 to 19,341
_A0017
Position (kb) 17
18
19 Strain fitness (log2 ratio) -2
-1
0
1
2 at 17.001 kb on + strand at 17.006 kb on + strand at 17.007 kb on - strand at 17.261 kb on + strand at 17.261 kb on + strand at 17.262 kb on - strand at 17.262 kb on - strand at 17.262 kb on - strand at 17.262 kb on - strand at 17.262 kb on - strand at 17.741 kb on - strand, within Rru_A0016 at 17.742 kb on + strand, within Rru_A0016 at 17.742 kb on + strand, within Rru_A0016 at 17.742 kb on + strand, within Rru_A0016 at 17.743 kb on - strand, within Rru_A0016 at 17.743 kb on - strand, within Rru_A0016 at 17.888 kb on - strand, within Rru_A0016 at 17.977 kb on + strand, within Rru_A0016 at 17.977 kb on + strand, within Rru_A0016 at 17.978 kb on - strand, within Rru_A0016 at 17.978 kb on - strand, within Rru_A0016 at 17.978 kb on - strand, within Rru_A0016 at 17.978 kb on - strand, within Rru_A0016 at 17.978 kb on - strand, within Rru_A0016 at 18.043 kb on + strand, within Rru_A0016 at 18.043 kb on + strand, within Rru_A0016 at 18.044 kb on - strand, within Rru_A0016 at 18.067 kb on + strand, within Rru_A0016 at 18.307 kb on + strand at 18.307 kb on + strand at 18.307 kb on + strand at 18.307 kb on + strand at 18.307 kb on + strand at 18.308 kb on - strand at 18.308 kb on - strand at 18.308 kb on - strand at 18.308 kb on - strand at 18.308 kb on - strand at 18.502 kb on + strand at 18.503 kb on - strand at 18.503 kb on - strand at 18.503 kb on - strand at 18.503 kb on - strand at 18.530 kb on + strand at 18.531 kb on - strand at 18.531 kb on - strand at 18.633 kb on - strand, within Rru_A0017 at 18.788 kb on + strand, within Rru_A0017 at 18.789 kb on - strand, within Rru_A0017 at 18.793 kb on + strand, within Rru_A0017 at 18.793 kb on + strand, within Rru_A0017 at 18.794 kb on - strand, within Rru_A0017 at 18.794 kb on - strand, within Rru_A0017 at 19.151 kb on + strand, within Rru_A0017 at 19.151 kb on + strand, within Rru_A0017 at 19.152 kb on - strand, within Rru_A0017 at 19.152 kb on - strand, within Rru_A0017 at 19.201 kb on + strand, within Rru_A0017 at 19.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 75 uM remove 17,001 + +0.0 17,006 + +0.0 17,007 - +0.8 17,261 + -0.4 17,261 + +0.0 17,262 - +0.8 17,262 - +2.2 17,262 - -0.1 17,262 - +1.4 17,262 - -0.3 17,741 - Rru_A0016 0.38 +0.3 17,742 + Rru_A0016 0.38 +0.2 17,742 + Rru_A0016 0.38 -0.3 17,742 + Rru_A0016 0.38 +0.0 17,743 - Rru_A0016 0.38 +1.1 17,743 - Rru_A0016 0.38 +1.0 17,888 - Rru_A0016 0.52 +0.6 17,977 + Rru_A0016 0.61 -0.1 17,977 + Rru_A0016 0.61 +0.0 17,978 - Rru_A0016 0.61 -0.4 17,978 - Rru_A0016 0.61 +0.9 17,978 - Rru_A0016 0.61 -1.7 17,978 - Rru_A0016 0.61 -1.2 17,978 - Rru_A0016 0.61 -0.2 18,043 + Rru_A0016 0.67 -0.3 18,043 + Rru_A0016 0.67 -1.8 18,044 - Rru_A0016 0.67 +1.0 18,067 + Rru_A0016 0.70 +1.4 18,307 + +0.6 18,307 + -0.5 18,307 + -0.6 18,307 + +0.0 18,307 + -0.9 18,308 - +0.0 18,308 - +0.0 18,308 - +0.2 18,308 - +0.0 18,308 - +0.0 18,502 + +0.0 18,503 - -0.1 18,503 - +1.0 18,503 - +0.0 18,503 - +0.0 18,530 + -0.2 18,531 - +0.1 18,531 - +0.4 18,633 - Rru_A0017 0.16 +1.0 18,788 + Rru_A0017 0.35 +0.0 18,789 - Rru_A0017 0.35 +0.0 18,793 + Rru_A0017 0.35 -1.0 18,793 + Rru_A0017 0.35 +1.0 18,794 - Rru_A0017 0.35 +0.1 18,794 - Rru_A0017 0.35 +0.9 19,151 + Rru_A0017 0.78 -1.0 19,151 + Rru_A0017 0.78 -0.2 19,152 - Rru_A0017 0.78 +0.0 19,152 - Rru_A0017 0.78 -0.3 19,201 + Rru_A0017 0.83 -1.0 19,361 - +0.7
Or see this region's nucleotide sequence