Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_978 and Pf6N2E2_979 are separated by 14 nucleotides Pf6N2E2_979 and Pf6N2E2_980 are separated by 159 nucleotides
Pf6N2E2_978 - Protein with ParB-like nuclease domain in PFGI-1-like cluster, at 1,145,870 to 1,147,453
_978
Pf6N2E2_979 - hypothetical protein, at 1,147,468 to 1,147,602
_979
Pf6N2E2_980 - Chromosome partitioning ATPase in PFGI-1-like cluster, ParA-like, at 1,147,762 to 1,148,598
_980
Position (kb)
1147
1148 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1146.575 kb on + strand, within Pf6N2E2_978 at 1146.648 kb on - strand, within Pf6N2E2_978 at 1146.836 kb on + strand, within Pf6N2E2_978 at 1146.837 kb on - strand, within Pf6N2E2_978 at 1146.872 kb on + strand, within Pf6N2E2_978 at 1146.872 kb on + strand, within Pf6N2E2_978 at 1147.203 kb on + strand, within Pf6N2E2_978 at 1147.203 kb on + strand, within Pf6N2E2_978 at 1147.204 kb on - strand, within Pf6N2E2_978 at 1147.204 kb on - strand, within Pf6N2E2_978 at 1147.281 kb on + strand, within Pf6N2E2_978 at 1147.348 kb on + strand at 1147.349 kb on - strand at 1147.355 kb on - strand at 1147.355 kb on - strand at 1147.588 kb on + strand, within Pf6N2E2_979 at 1147.659 kb on + strand at 1147.659 kb on + strand at 1147.660 kb on - strand at 1147.660 kb on - strand at 1147.743 kb on + strand at 1147.743 kb on + strand at 1147.791 kb on + strand at 1147.791 kb on + strand at 1147.951 kb on + strand, within Pf6N2E2_980 at 1147.951 kb on + strand, within Pf6N2E2_980 at 1147.951 kb on + strand, within Pf6N2E2_980 at 1147.951 kb on + strand, within Pf6N2E2_980 at 1147.952 kb on - strand, within Pf6N2E2_980 at 1147.961 kb on - strand, within Pf6N2E2_980 at 1148.053 kb on + strand, within Pf6N2E2_980 at 1148.163 kb on + strand, within Pf6N2E2_980 at 1148.163 kb on + strand, within Pf6N2E2_980 at 1148.164 kb on - strand, within Pf6N2E2_980 at 1148.212 kb on + strand, within Pf6N2E2_980 at 1148.212 kb on + strand, within Pf6N2E2_980 at 1148.217 kb on + strand, within Pf6N2E2_980 at 1148.217 kb on + strand, within Pf6N2E2_980 at 1148.218 kb on - strand, within Pf6N2E2_980 at 1148.390 kb on - strand, within Pf6N2E2_980 at 1148.463 kb on - strand, within Pf6N2E2_980 at 1148.463 kb on - strand, within Pf6N2E2_980 at 1148.465 kb on + strand, within Pf6N2E2_980 at 1148.594 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 1,146,575 + Pf6N2E2_978 0.45 -2.0 1,146,648 - Pf6N2E2_978 0.49 -0.9 1,146,836 + Pf6N2E2_978 0.61 +2.0 1,146,837 - Pf6N2E2_978 0.61 -1.5 1,146,872 + Pf6N2E2_978 0.63 +0.7 1,146,872 + Pf6N2E2_978 0.63 -1.3 1,147,203 + Pf6N2E2_978 0.84 +0.6 1,147,203 + Pf6N2E2_978 0.84 -1.6 1,147,204 - Pf6N2E2_978 0.84 +1.3 1,147,204 - Pf6N2E2_978 0.84 -2.7 1,147,281 + Pf6N2E2_978 0.89 -1.3 1,147,348 + +1.9 1,147,349 - -1.5 1,147,355 - -0.1 1,147,355 - +0.6 1,147,588 + Pf6N2E2_979 0.89 -1.3 1,147,659 + +1.1 1,147,659 + +0.7 1,147,660 - +1.1 1,147,660 - -0.4 1,147,743 + +1.5 1,147,743 + +1.3 1,147,791 + -0.9 1,147,791 + -2.4 1,147,951 + Pf6N2E2_980 0.23 +1.8 1,147,951 + Pf6N2E2_980 0.23 -3.1 1,147,951 + Pf6N2E2_980 0.23 +3.1 1,147,951 + Pf6N2E2_980 0.23 +2.2 1,147,952 - Pf6N2E2_980 0.23 -2.0 1,147,961 - Pf6N2E2_980 0.24 -0.5 1,148,053 + Pf6N2E2_980 0.35 +0.7 1,148,163 + Pf6N2E2_980 0.48 +0.2 1,148,163 + Pf6N2E2_980 0.48 +0.5 1,148,164 - Pf6N2E2_980 0.48 -1.1 1,148,212 + Pf6N2E2_980 0.54 -2.6 1,148,212 + Pf6N2E2_980 0.54 +1.1 1,148,217 + Pf6N2E2_980 0.54 +1.1 1,148,217 + Pf6N2E2_980 0.54 -0.9 1,148,218 - Pf6N2E2_980 0.54 +1.4 1,148,390 - Pf6N2E2_980 0.75 -1.4 1,148,463 - Pf6N2E2_980 0.84 -2.1 1,148,463 - Pf6N2E2_980 0.84 +1.9 1,148,465 + Pf6N2E2_980 0.84 -2.1 1,148,594 - -2.5
Or see this region's nucleotide sequence