Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_876 and Pf6N2E2_877 are separated by 19 nucleotides Pf6N2E2_877 and Pf6N2E2_878 are separated by 76 nucleotides Pf6N2E2_878 and Pf6N2E2_879 are separated by 238 nucleotides Pf6N2E2_879 and Pf6N2E2_880 are separated by 106 nucleotides
Pf6N2E2_876 - Cobalt-containing nitrile hydratase subunit beta (EC 4.2.1.84), at 1,025,643 to 1,026,296
_876
Pf6N2E2_877 - Cobalt-containing nitrile hydratase subunit alpha (EC 4.2.1.84), at 1,026,316 to 1,026,948
_877
Pf6N2E2_878 - Amidase clustered with urea ABC transporter and nitrile hydratase functions, at 1,027,025 to 1,028,539
_878
Pf6N2E2_879 - Cyanoglobin; Hemoglobin-like protein HbN, at 1,028,778 to 1,029,302
_879
Pf6N2E2_880 - QbdB, at 1,029,409 to 1,030,275
_880
Position (kb)
1027
1028
1029 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 1026.092 kb on - strand, within Pf6N2E2_876 at 1026.095 kb on - strand, within Pf6N2E2_876 at 1026.095 kb on - strand, within Pf6N2E2_876 at 1026.109 kb on + strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876 at 1026.269 kb on + strand at 1026.269 kb on + strand at 1026.270 kb on - strand at 1026.277 kb on - strand at 1026.286 kb on - strand at 1026.434 kb on - strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.473 kb on - strand, within Pf6N2E2_877 at 1026.493 kb on - strand, within Pf6N2E2_877 at 1026.493 kb on - strand, within Pf6N2E2_877 at 1026.563 kb on + strand, within Pf6N2E2_877 at 1026.563 kb on + strand, within Pf6N2E2_877 at 1026.737 kb on + strand, within Pf6N2E2_877 at 1026.738 kb on - strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.940 kb on - strand at 1026.953 kb on + strand at 1026.953 kb on + strand at 1026.954 kb on - strand at 1026.971 kb on + strand at 1026.987 kb on + strand at 1026.987 kb on + strand at 1026.988 kb on - strand at 1026.988 kb on - strand at 1026.988 kb on - strand at 1027.026 kb on + strand at 1027.027 kb on - strand at 1027.092 kb on + strand at 1027.171 kb on + strand at 1027.296 kb on + strand, within Pf6N2E2_878 at 1027.296 kb on + strand, within Pf6N2E2_878 at 1027.296 kb on + strand, within Pf6N2E2_878 at 1027.297 kb on - strand, within Pf6N2E2_878 at 1027.297 kb on - strand, within Pf6N2E2_878 at 1027.297 kb on - strand, within Pf6N2E2_878 at 1027.297 kb on - strand, within Pf6N2E2_878 at 1027.318 kb on - strand, within Pf6N2E2_878 at 1027.360 kb on - strand, within Pf6N2E2_878 at 1027.374 kb on + strand, within Pf6N2E2_878 at 1027.375 kb on - strand, within Pf6N2E2_878 at 1027.375 kb on - strand, within Pf6N2E2_878 at 1027.375 kb on - strand, within Pf6N2E2_878 at 1027.482 kb on + strand, within Pf6N2E2_878 at 1027.483 kb on - strand, within Pf6N2E2_878 at 1027.746 kb on + strand, within Pf6N2E2_878 at 1027.746 kb on + strand, within Pf6N2E2_878 at 1027.746 kb on + strand, within Pf6N2E2_878 at 1027.890 kb on + strand, within Pf6N2E2_878 at 1027.890 kb on + strand, within Pf6N2E2_878 at 1027.890 kb on + strand, within Pf6N2E2_878 at 1027.890 kb on + strand, within Pf6N2E2_878 at 1027.891 kb on - strand, within Pf6N2E2_878 at 1027.891 kb on - strand, within Pf6N2E2_878 at 1027.898 kb on + strand, within Pf6N2E2_878 at 1027.899 kb on - strand, within Pf6N2E2_878 at 1027.909 kb on + strand, within Pf6N2E2_878 at 1027.909 kb on + strand, within Pf6N2E2_878 at 1027.909 kb on + strand, within Pf6N2E2_878 at 1027.910 kb on - strand, within Pf6N2E2_878 at 1027.910 kb on - strand, within Pf6N2E2_878 at 1027.910 kb on - strand, within Pf6N2E2_878 at 1027.910 kb on - strand, within Pf6N2E2_878 at 1027.923 kb on + strand, within Pf6N2E2_878 at 1027.924 kb on - strand, within Pf6N2E2_878 at 1027.924 kb on - strand, within Pf6N2E2_878 at 1027.924 kb on - strand, within Pf6N2E2_878 at 1027.924 kb on - strand, within Pf6N2E2_878 at 1028.041 kb on + strand, within Pf6N2E2_878 at 1028.041 kb on + strand, within Pf6N2E2_878 at 1028.041 kb on + strand, within Pf6N2E2_878 at 1028.041 kb on + strand, within Pf6N2E2_878 at 1028.041 kb on + strand, within Pf6N2E2_878 at 1028.042 kb on - strand, within Pf6N2E2_878 at 1028.042 kb on - strand, within Pf6N2E2_878 at 1028.042 kb on - strand, within Pf6N2E2_878 at 1028.042 kb on - strand, within Pf6N2E2_878 at 1028.042 kb on - strand, within Pf6N2E2_878 at 1028.044 kb on - strand, within Pf6N2E2_878 at 1028.086 kb on + strand, within Pf6N2E2_878 at 1028.087 kb on - strand, within Pf6N2E2_878 at 1028.087 kb on - strand, within Pf6N2E2_878 at 1028.109 kb on + strand, within Pf6N2E2_878 at 1028.109 kb on + strand, within Pf6N2E2_878 at 1028.189 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.245 kb on + strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.246 kb on - strand, within Pf6N2E2_878 at 1028.313 kb on + strand, within Pf6N2E2_878 at 1028.314 kb on - strand, within Pf6N2E2_878 at 1028.316 kb on + strand, within Pf6N2E2_878 at 1028.317 kb on - strand, within Pf6N2E2_878 at 1028.418 kb on + strand at 1028.452 kb on - strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.481 kb on - strand at 1028.481 kb on - strand at 1028.481 kb on - strand at 1028.481 kb on - strand at 1028.654 kb on - strand at 1028.661 kb on + strand at 1028.661 kb on + strand at 1028.661 kb on + strand at 1028.662 kb on - strand at 1028.662 kb on - strand at 1028.662 kb on - strand at 1028.759 kb on + strand at 1028.760 kb on - strand at 1028.760 kb on - strand at 1028.765 kb on + strand at 1028.765 kb on + strand at 1028.765 kb on + strand at 1028.766 kb on - strand at 1028.766 kb on - strand at 1028.992 kb on + strand, within Pf6N2E2_879 at 1028.993 kb on - strand, within Pf6N2E2_879 at 1029.082 kb on + strand, within Pf6N2E2_879 at 1029.083 kb on - strand, within Pf6N2E2_879 at 1029.161 kb on + strand, within Pf6N2E2_879 at 1029.161 kb on + strand, within Pf6N2E2_879 at 1029.161 kb on + strand, within Pf6N2E2_879 at 1029.161 kb on + strand, within Pf6N2E2_879 at 1029.162 kb on - strand, within Pf6N2E2_879 at 1029.162 kb on - strand, within Pf6N2E2_879 at 1029.162 kb on - strand, within Pf6N2E2_879 at 1029.162 kb on - strand, within Pf6N2E2_879 at 1029.210 kb on + strand, within Pf6N2E2_879 at 1029.229 kb on + strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.242 kb on - strand, within Pf6N2E2_879 at 1029.258 kb on - strand at 1029.259 kb on + strand at 1029.259 kb on + strand at 1029.260 kb on - strand at 1029.260 kb on - strand at 1029.260 kb on - strand at 1029.260 kb on - strand at 1029.379 kb on - strand at 1029.395 kb on - strand at 1029.411 kb on - strand at 1029.426 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 1,026,092 - Pf6N2E2_876 0.69 +1.3 1,026,095 - Pf6N2E2_876 0.69 +0.4 1,026,095 - Pf6N2E2_876 0.69 -2.9 1,026,109 + Pf6N2E2_876 0.71 -1.0 1,026,110 - Pf6N2E2_876 0.71 -0.8 1,026,110 - Pf6N2E2_876 0.71 +0.7 1,026,110 - Pf6N2E2_876 0.71 +1.1 1,026,269 + +0.6 1,026,269 + +1.7 1,026,270 - -0.0 1,026,277 - -0.8 1,026,286 - -0.2 1,026,434 - Pf6N2E2_877 0.19 -0.2 1,026,472 + Pf6N2E2_877 0.25 +1.0 1,026,472 + Pf6N2E2_877 0.25 -0.0 1,026,472 + Pf6N2E2_877 0.25 -1.3 1,026,472 + Pf6N2E2_877 0.25 +1.1 1,026,473 - Pf6N2E2_877 0.25 +0.6 1,026,493 - Pf6N2E2_877 0.28 +0.6 1,026,493 - Pf6N2E2_877 0.28 +0.6 1,026,563 + Pf6N2E2_877 0.39 -2.0 1,026,563 + Pf6N2E2_877 0.39 +0.7 1,026,737 + Pf6N2E2_877 0.67 -0.3 1,026,738 - Pf6N2E2_877 0.67 +1.0 1,026,803 + Pf6N2E2_877 0.77 -2.3 1,026,803 + Pf6N2E2_877 0.77 -1.3 1,026,803 + Pf6N2E2_877 0.77 -2.3 1,026,803 + Pf6N2E2_877 0.77 -3.4 1,026,803 + Pf6N2E2_877 0.77 +2.6 1,026,803 + Pf6N2E2_877 0.77 +0.4 1,026,804 - Pf6N2E2_877 0.77 -2.3 1,026,804 - Pf6N2E2_877 0.77 +0.7 1,026,804 - Pf6N2E2_877 0.77 -1.9 1,026,804 - Pf6N2E2_877 0.77 +1.3 1,026,804 - Pf6N2E2_877 0.77 +2.4 1,026,804 - Pf6N2E2_877 0.77 +0.6 1,026,940 - -2.0 1,026,953 + +1.1 1,026,953 + -1.0 1,026,954 - +2.2 1,026,971 + +1.7 1,026,987 + +0.2 1,026,987 + +0.6 1,026,988 - -1.0 1,026,988 - -0.9 1,026,988 - -1.4 1,027,026 + +0.8 1,027,027 - -2.1 1,027,092 + -0.2 1,027,171 + +1.4 1,027,296 + Pf6N2E2_878 0.18 +2.7 1,027,296 + Pf6N2E2_878 0.18 -1.9 1,027,296 + Pf6N2E2_878 0.18 -1.4 1,027,297 - Pf6N2E2_878 0.18 -0.4 1,027,297 - Pf6N2E2_878 0.18 +1.8 1,027,297 - Pf6N2E2_878 0.18 +2.4 1,027,297 - Pf6N2E2_878 0.18 -2.1 1,027,318 - Pf6N2E2_878 0.19 -0.4 1,027,360 - Pf6N2E2_878 0.22 +1.6 1,027,374 + Pf6N2E2_878 0.23 -1.5 1,027,375 - Pf6N2E2_878 0.23 +1.1 1,027,375 - Pf6N2E2_878 0.23 -1.1 1,027,375 - Pf6N2E2_878 0.23 -1.3 1,027,482 + Pf6N2E2_878 0.30 -1.8 1,027,483 - Pf6N2E2_878 0.30 -1.4 1,027,746 + Pf6N2E2_878 0.48 -0.8 1,027,746 + Pf6N2E2_878 0.48 -1.6 1,027,746 + Pf6N2E2_878 0.48 +1.9 1,027,890 + Pf6N2E2_878 0.57 -0.5 1,027,890 + Pf6N2E2_878 0.57 +0.6 1,027,890 + Pf6N2E2_878 0.57 +1.9 1,027,890 + Pf6N2E2_878 0.57 -2.1 1,027,891 - Pf6N2E2_878 0.57 +2.4 1,027,891 - Pf6N2E2_878 0.57 -3.3 1,027,898 + Pf6N2E2_878 0.58 -3.7 1,027,899 - Pf6N2E2_878 0.58 -1.0 1,027,909 + Pf6N2E2_878 0.58 +1.0 1,027,909 + Pf6N2E2_878 0.58 +1.6 1,027,909 + Pf6N2E2_878 0.58 +1.9 1,027,910 - Pf6N2E2_878 0.58 -2.0 1,027,910 - Pf6N2E2_878 0.58 -0.4 1,027,910 - Pf6N2E2_878 0.58 +1.1 1,027,910 - Pf6N2E2_878 0.58 -2.4 1,027,923 + Pf6N2E2_878 0.59 -0.7 1,027,924 - Pf6N2E2_878 0.59 +3.6 1,027,924 - Pf6N2E2_878 0.59 -2.8 1,027,924 - Pf6N2E2_878 0.59 +2.3 1,027,924 - Pf6N2E2_878 0.59 -0.2 1,028,041 + Pf6N2E2_878 0.67 -2.1 1,028,041 + Pf6N2E2_878 0.67 +0.2 1,028,041 + Pf6N2E2_878 0.67 +2.0 1,028,041 + Pf6N2E2_878 0.67 -0.0 1,028,041 + Pf6N2E2_878 0.67 +0.4 1,028,042 - Pf6N2E2_878 0.67 +0.7 1,028,042 - Pf6N2E2_878 0.67 -1.3 1,028,042 - Pf6N2E2_878 0.67 +1.1 1,028,042 - Pf6N2E2_878 0.67 +2.3 1,028,042 - Pf6N2E2_878 0.67 -1.6 1,028,044 - Pf6N2E2_878 0.67 -2.4 1,028,086 + Pf6N2E2_878 0.70 +0.2 1,028,087 - Pf6N2E2_878 0.70 +1.8 1,028,087 - Pf6N2E2_878 0.70 +1.4 1,028,109 + Pf6N2E2_878 0.72 -1.5 1,028,109 + Pf6N2E2_878 0.72 -0.3 1,028,189 + Pf6N2E2_878 0.77 +2.0 1,028,245 + Pf6N2E2_878 0.81 +2.0 1,028,245 + Pf6N2E2_878 0.81 -0.0 1,028,245 + Pf6N2E2_878 0.81 -2.9 1,028,245 + Pf6N2E2_878 0.81 +0.7 1,028,245 + Pf6N2E2_878 0.81 +1.1 1,028,245 + Pf6N2E2_878 0.81 +0.1 1,028,245 + Pf6N2E2_878 0.81 +1.2 1,028,245 + Pf6N2E2_878 0.81 -2.9 1,028,245 + Pf6N2E2_878 0.81 -2.0 1,028,245 + Pf6N2E2_878 0.81 +2.0 1,028,246 - Pf6N2E2_878 0.81 -1.9 1,028,246 - Pf6N2E2_878 0.81 -2.3 1,028,246 - Pf6N2E2_878 0.81 -0.9 1,028,246 - Pf6N2E2_878 0.81 -2.5 1,028,246 - Pf6N2E2_878 0.81 +1.1 1,028,246 - Pf6N2E2_878 0.81 +1.4 1,028,246 - Pf6N2E2_878 0.81 -1.0 1,028,246 - Pf6N2E2_878 0.81 +1.7 1,028,246 - Pf6N2E2_878 0.81 -2.8 1,028,246 - Pf6N2E2_878 0.81 -2.0 1,028,246 - Pf6N2E2_878 0.81 +1.8 1,028,246 - Pf6N2E2_878 0.81 -0.8 1,028,246 - Pf6N2E2_878 0.81 +1.0 1,028,246 - Pf6N2E2_878 0.81 +1.1 1,028,313 + Pf6N2E2_878 0.85 +1.3 1,028,314 - Pf6N2E2_878 0.85 -1.8 1,028,316 + Pf6N2E2_878 0.85 +0.6 1,028,317 - Pf6N2E2_878 0.85 -0.1 1,028,418 + -1.5 1,028,452 - +1.4 1,028,480 + -0.9 1,028,480 + -0.3 1,028,480 + +0.4 1,028,480 + +0.8 1,028,480 + +0.3 1,028,480 + +0.2 1,028,480 + +1.8 1,028,481 - -0.0 1,028,481 - -1.9 1,028,481 - -2.4 1,028,481 - -0.4 1,028,654 - -1.1 1,028,661 + -0.7 1,028,661 + -1.6 1,028,661 + -0.6 1,028,662 - -3.1 1,028,662 - +2.6 1,028,662 - +2.8 1,028,759 + +1.3 1,028,760 - +0.7 1,028,760 - +0.9 1,028,765 + -1.3 1,028,765 + +1.8 1,028,765 + +0.3 1,028,766 - -1.9 1,028,766 - +0.1 1,028,992 + Pf6N2E2_879 0.41 -1.0 1,028,993 - Pf6N2E2_879 0.41 -1.8 1,029,082 + Pf6N2E2_879 0.58 -1.5 1,029,083 - Pf6N2E2_879 0.58 -1.8 1,029,161 + Pf6N2E2_879 0.73 +0.3 1,029,161 + Pf6N2E2_879 0.73 +2.6 1,029,161 + Pf6N2E2_879 0.73 -1.9 1,029,161 + Pf6N2E2_879 0.73 +0.1 1,029,162 - Pf6N2E2_879 0.73 +5.9 1,029,162 - Pf6N2E2_879 0.73 -2.1 1,029,162 - Pf6N2E2_879 0.73 -0.0 1,029,162 - Pf6N2E2_879 0.73 +3.4 1,029,210 + Pf6N2E2_879 0.82 +0.3 1,029,229 + Pf6N2E2_879 0.86 +0.8 1,029,230 - Pf6N2E2_879 0.86 +1.0 1,029,230 - Pf6N2E2_879 0.86 +1.2 1,029,230 - Pf6N2E2_879 0.86 +0.2 1,029,230 - Pf6N2E2_879 0.86 -1.0 1,029,230 - Pf6N2E2_879 0.86 -1.1 1,029,242 - Pf6N2E2_879 0.88 -2.8 1,029,258 - +0.3 1,029,259 + -0.8 1,029,259 + -0.1 1,029,260 - -2.4 1,029,260 - -0.4 1,029,260 - -1.0 1,029,260 - -2.1 1,029,379 - +0.3 1,029,395 - -0.2 1,029,411 - +0.6 1,029,426 - +1.0
Or see this region's nucleotide sequence