Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_681 and Pf6N2E2_682 are separated by 112 nucleotides Pf6N2E2_682 and Pf6N2E2_683 are separated by 203 nucleotides
Pf6N2E2_681 - 4-hydroxyproline epimerase (EC 5.1.1.8), at 785,832 to 786,764
_681
Pf6N2E2_682 - Predicted regulator PutR for proline utilization, GntR family, at 786,877 to 787,548
_682
Pf6N2E2_683 - Probable MFS transporter, at 787,752 to 788,918
_683
Position (kb) 786
787
788 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 785.947 kb on + strand, within Pf6N2E2_681 at 785.947 kb on + strand, within Pf6N2E2_681 at 786.073 kb on + strand, within Pf6N2E2_681 at 786.074 kb on - strand, within Pf6N2E2_681 at 786.093 kb on + strand, within Pf6N2E2_681 at 786.093 kb on + strand, within Pf6N2E2_681 at 786.198 kb on + strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.211 kb on + strand, within Pf6N2E2_681 at 786.355 kb on + strand, within Pf6N2E2_681 at 786.355 kb on + strand, within Pf6N2E2_681 at 786.408 kb on + strand, within Pf6N2E2_681 at 786.832 kb on - strand at 786.832 kb on - strand at 786.837 kb on + strand at 786.837 kb on + strand at 786.838 kb on - strand at 786.838 kb on - strand at 786.838 kb on - strand at 787.115 kb on + strand, within Pf6N2E2_682 at 787.116 kb on - strand, within Pf6N2E2_682 at 787.116 kb on - strand, within Pf6N2E2_682 at 787.116 kb on - strand, within Pf6N2E2_682 at 787.160 kb on - strand, within Pf6N2E2_682 at 787.160 kb on - strand, within Pf6N2E2_682 at 787.160 kb on - strand, within Pf6N2E2_682 at 787.160 kb on - strand, within Pf6N2E2_682 at 787.246 kb on + strand, within Pf6N2E2_682 at 787.247 kb on - strand, within Pf6N2E2_682 at 787.585 kb on + strand at 787.777 kb on + strand at 787.777 kb on + strand at 787.777 kb on + strand at 787.777 kb on + strand at 787.778 kb on - strand at 787.778 kb on - strand at 787.778 kb on - strand at 787.778 kb on - strand at 787.778 kb on - strand at 787.778 kb on - strand at 787.778 kb on - strand at 787.918 kb on + strand, within Pf6N2E2_683 at 787.919 kb on - strand, within Pf6N2E2_683 at 787.999 kb on + strand, within Pf6N2E2_683 at 788.092 kb on + strand, within Pf6N2E2_683 at 788.190 kb on - strand, within Pf6N2E2_683
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 785,947 + Pf6N2E2_681 0.12 +2.9 785,947 + Pf6N2E2_681 0.12 -1.1 786,073 + Pf6N2E2_681 0.26 +1.9 786,074 - Pf6N2E2_681 0.26 +2.1 786,093 + Pf6N2E2_681 0.28 +0.7 786,093 + Pf6N2E2_681 0.28 +0.1 786,198 + Pf6N2E2_681 0.39 -2.2 786,206 - Pf6N2E2_681 0.40 +0.1 786,206 - Pf6N2E2_681 0.40 +0.5 786,206 - Pf6N2E2_681 0.40 -3.0 786,211 + Pf6N2E2_681 0.41 +2.3 786,355 + Pf6N2E2_681 0.56 -3.3 786,355 + Pf6N2E2_681 0.56 +1.6 786,408 + Pf6N2E2_681 0.62 +2.9 786,832 - -2.5 786,832 - -0.1 786,837 + -1.1 786,837 + -0.8 786,838 - +0.4 786,838 - -1.4 786,838 - -1.0 787,115 + Pf6N2E2_682 0.35 -1.1 787,116 - Pf6N2E2_682 0.36 +0.9 787,116 - Pf6N2E2_682 0.36 +1.8 787,116 - Pf6N2E2_682 0.36 +1.3 787,160 - Pf6N2E2_682 0.42 -2.9 787,160 - Pf6N2E2_682 0.42 -0.4 787,160 - Pf6N2E2_682 0.42 -1.1 787,160 - Pf6N2E2_682 0.42 +2.9 787,246 + Pf6N2E2_682 0.55 +1.8 787,247 - Pf6N2E2_682 0.55 +0.9 787,585 + -1.8 787,777 + -0.7 787,777 + -1.0 787,777 + -0.8 787,777 + +1.1 787,778 - +2.7 787,778 - +1.1 787,778 - -0.4 787,778 - +0.6 787,778 - +1.0 787,778 - -0.3 787,778 - +0.2 787,918 + Pf6N2E2_683 0.14 -0.3 787,919 - Pf6N2E2_683 0.14 +2.0 787,999 + Pf6N2E2_683 0.21 -2.6 788,092 + Pf6N2E2_683 0.29 -2.1 788,190 - Pf6N2E2_683 0.38 -0.2
Or see this region's nucleotide sequence