Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_663 and Pf6N2E2_664 are separated by 342 nucleotides Pf6N2E2_664 and Pf6N2E2_665 are separated by 2 nucleotides
Pf6N2E2_663 - Transcriptional activator of acetoin dehydrogenase operon AcoR, at 766,744 to 768,609
_663
Pf6N2E2_664 - Acetoin catabolism protein X, at 768,952 to 770,025
_664
Pf6N2E2_665 - Acetoin dehydrogenase E1 component alpha-subunit (EC 1.2.4.-), at 770,028 to 771,005
_665
Position (kb) 768
769
770
771 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 768.248 kb on + strand, within Pf6N2E2_663 at 768.609 kb on + strand at 768.610 kb on - strand at 768.649 kb on + strand at 768.887 kb on - strand at 768.887 kb on - strand at 768.890 kb on - strand at 768.904 kb on + strand at 768.905 kb on - strand at 768.905 kb on - strand at 769.432 kb on + strand, within Pf6N2E2_664 at 769.433 kb on - strand, within Pf6N2E2_664 at 769.433 kb on - strand, within Pf6N2E2_664 at 769.939 kb on + strand at 770.155 kb on - strand, within Pf6N2E2_665 at 770.155 kb on - strand, within Pf6N2E2_665 at 770.289 kb on + strand, within Pf6N2E2_665 at 770.289 kb on + strand, within Pf6N2E2_665 at 770.290 kb on - strand, within Pf6N2E2_665 at 770.560 kb on - strand, within Pf6N2E2_665 at 770.934 kb on + strand at 770.988 kb on + strand at 770.989 kb on - strand at 770.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 768,248 + Pf6N2E2_663 0.81 -0.8 768,609 + -0.0 768,610 - +1.4 768,649 + +1.2 768,887 - +0.7 768,887 - -0.1 768,890 - +0.9 768,904 + -0.3 768,905 - -1.5 768,905 - +1.3 769,432 + Pf6N2E2_664 0.45 -1.5 769,433 - Pf6N2E2_664 0.45 -1.3 769,433 - Pf6N2E2_664 0.45 -1.1 769,939 + -0.8 770,155 - Pf6N2E2_665 0.13 +0.8 770,155 - Pf6N2E2_665 0.13 +1.5 770,289 + Pf6N2E2_665 0.27 +3.3 770,289 + Pf6N2E2_665 0.27 -0.2 770,290 - Pf6N2E2_665 0.27 -2.4 770,560 - Pf6N2E2_665 0.54 -3.1 770,934 + -1.7 770,988 + +0.1 770,989 - -2.1 770,998 - -1.8
Or see this region's nucleotide sequence