Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_580 and Pf6N2E2_581 are separated by 19 nucleotides Pf6N2E2_581 and Pf6N2E2_582 are separated by 1 nucleotides
Pf6N2E2_580 - Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9), at 655,017 to 655,811
_580
Pf6N2E2_581 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 655,831 to 657,441
_581
Pf6N2E2_582 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 657,443 to 658,462
_582
Position (kb)
655
656
657
658 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 654.865 kb on + strand at 654.866 kb on - strand at 654.873 kb on + strand at 654.873 kb on + strand at 654.873 kb on + strand at 654.873 kb on + strand at 654.873 kb on + strand at 654.873 kb on + strand at 654.873 kb on + strand at 654.925 kb on + strand at 654.925 kb on + strand at 654.926 kb on - strand at 654.936 kb on - strand at 654.936 kb on - strand at 654.936 kb on - strand at 655.018 kb on + strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.884 kb on - strand at 655.884 kb on - strand at 656.010 kb on - strand, within Pf6N2E2_581 at 656.010 kb on - strand, within Pf6N2E2_581 at 656.010 kb on - strand, within Pf6N2E2_581 at 656.022 kb on - strand, within Pf6N2E2_581 at 656.022 kb on - strand, within Pf6N2E2_581 at 656.022 kb on - strand, within Pf6N2E2_581 at 656.071 kb on - strand, within Pf6N2E2_581 at 656.210 kb on - strand, within Pf6N2E2_581 at 656.300 kb on - strand, within Pf6N2E2_581 at 656.300 kb on - strand, within Pf6N2E2_581 at 656.300 kb on - strand, within Pf6N2E2_581 at 656.550 kb on - strand, within Pf6N2E2_581 at 656.550 kb on - strand, within Pf6N2E2_581 at 656.550 kb on - strand, within Pf6N2E2_581 at 656.550 kb on - strand, within Pf6N2E2_581 at 656.550 kb on - strand, within Pf6N2E2_581 at 656.550 kb on - strand, within Pf6N2E2_581 at 656.550 kb on - strand, within Pf6N2E2_581 at 656.758 kb on + strand, within Pf6N2E2_581 at 656.758 kb on + strand, within Pf6N2E2_581 at 656.758 kb on + strand, within Pf6N2E2_581 at 656.758 kb on + strand, within Pf6N2E2_581 at 656.758 kb on + strand, within Pf6N2E2_581 at 656.758 kb on + strand, within Pf6N2E2_581 at 656.758 kb on + strand, within Pf6N2E2_581 at 656.759 kb on - strand, within Pf6N2E2_581 at 656.759 kb on - strand, within Pf6N2E2_581 at 656.759 kb on - strand, within Pf6N2E2_581 at 656.759 kb on - strand, within Pf6N2E2_581 at 657.221 kb on + strand, within Pf6N2E2_581 at 657.221 kb on + strand, within Pf6N2E2_581 at 657.222 kb on - strand, within Pf6N2E2_581 at 657.430 kb on - strand at 657.762 kb on + strand, within Pf6N2E2_582 at 657.763 kb on - strand, within Pf6N2E2_582 at 657.894 kb on + strand, within Pf6N2E2_582 at 657.960 kb on + strand, within Pf6N2E2_582 at 657.960 kb on + strand, within Pf6N2E2_582 at 657.961 kb on - strand, within Pf6N2E2_582 at 658.028 kb on + strand, within Pf6N2E2_582 at 658.029 kb on - strand, within Pf6N2E2_582 at 658.029 kb on - strand, within Pf6N2E2_582 at 658.029 kb on - strand, within Pf6N2E2_582 at 658.029 kb on - strand, within Pf6N2E2_582 at 658.050 kb on + strand, within Pf6N2E2_582 at 658.050 kb on + strand, within Pf6N2E2_582 at 658.050 kb on + strand, within Pf6N2E2_582 at 658.051 kb on - strand, within Pf6N2E2_582 at 658.051 kb on - strand, within Pf6N2E2_582 at 658.144 kb on - strand, within Pf6N2E2_582 at 658.159 kb on - strand, within Pf6N2E2_582 at 658.159 kb on - strand, within Pf6N2E2_582 at 658.159 kb on - strand, within Pf6N2E2_582 at 658.221 kb on + strand, within Pf6N2E2_582 at 658.221 kb on + strand, within Pf6N2E2_582 at 658.222 kb on - strand, within Pf6N2E2_582 at 658.222 kb on - strand, within Pf6N2E2_582 at 658.222 kb on - strand, within Pf6N2E2_582 at 658.222 kb on - strand, within Pf6N2E2_582 at 658.222 kb on - strand, within Pf6N2E2_582 at 658.233 kb on + strand, within Pf6N2E2_582 at 658.233 kb on + strand, within Pf6N2E2_582 at 658.233 kb on + strand, within Pf6N2E2_582 at 658.234 kb on - strand, within Pf6N2E2_582 at 658.314 kb on + strand, within Pf6N2E2_582 at 658.315 kb on - strand, within Pf6N2E2_582
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 654,865 + +1.7 654,866 - +1.0 654,873 + +1.5 654,873 + +0.2 654,873 + +0.8 654,873 + +0.3 654,873 + +0.5 654,873 + +1.3 654,873 + -0.9 654,925 + +1.4 654,925 + -0.3 654,926 - +1.0 654,936 - -1.2 654,936 - +0.1 654,936 - -1.0 655,018 + +0.1 655,882 - -0.8 655,882 - +0.3 655,882 - -2.4 655,882 - -0.8 655,882 - +0.1 655,882 - -3.3 655,882 - -0.5 655,882 - +0.3 655,882 - +0.0 655,884 - -0.2 655,884 - -1.2 656,010 - Pf6N2E2_581 0.11 +1.9 656,010 - Pf6N2E2_581 0.11 -0.8 656,010 - Pf6N2E2_581 0.11 -2.2 656,022 - Pf6N2E2_581 0.12 -1.2 656,022 - Pf6N2E2_581 0.12 +1.8 656,022 - Pf6N2E2_581 0.12 -2.8 656,071 - Pf6N2E2_581 0.15 -2.6 656,210 - Pf6N2E2_581 0.24 +0.9 656,300 - Pf6N2E2_581 0.29 -1.9 656,300 - Pf6N2E2_581 0.29 +0.6 656,300 - Pf6N2E2_581 0.29 -1.8 656,550 - Pf6N2E2_581 0.45 +1.4 656,550 - Pf6N2E2_581 0.45 -1.3 656,550 - Pf6N2E2_581 0.45 +0.1 656,550 - Pf6N2E2_581 0.45 -1.9 656,550 - Pf6N2E2_581 0.45 +0.8 656,550 - Pf6N2E2_581 0.45 +0.9 656,550 - Pf6N2E2_581 0.45 -1.2 656,758 + Pf6N2E2_581 0.58 +0.4 656,758 + Pf6N2E2_581 0.58 -2.6 656,758 + Pf6N2E2_581 0.58 -1.1 656,758 + Pf6N2E2_581 0.58 -1.3 656,758 + Pf6N2E2_581 0.58 -1.1 656,758 + Pf6N2E2_581 0.58 -0.4 656,758 + Pf6N2E2_581 0.58 -0.9 656,759 - Pf6N2E2_581 0.58 +0.2 656,759 - Pf6N2E2_581 0.58 -0.0 656,759 - Pf6N2E2_581 0.58 -2.5 656,759 - Pf6N2E2_581 0.58 +0.7 657,221 + Pf6N2E2_581 0.86 -1.4 657,221 + Pf6N2E2_581 0.86 -0.1 657,222 - Pf6N2E2_581 0.86 -0.4 657,430 - +1.6 657,762 + Pf6N2E2_582 0.31 +0.8 657,763 - Pf6N2E2_582 0.31 +0.6 657,894 + Pf6N2E2_582 0.44 -2.0 657,960 + Pf6N2E2_582 0.51 -0.2 657,960 + Pf6N2E2_582 0.51 -0.8 657,961 - Pf6N2E2_582 0.51 -0.3 658,028 + Pf6N2E2_582 0.57 -2.2 658,029 - Pf6N2E2_582 0.57 -1.9 658,029 - Pf6N2E2_582 0.57 -1.4 658,029 - Pf6N2E2_582 0.57 -3.3 658,029 - Pf6N2E2_582 0.57 -2.8 658,050 + Pf6N2E2_582 0.60 +0.6 658,050 + Pf6N2E2_582 0.60 +0.6 658,050 + Pf6N2E2_582 0.60 -2.0 658,051 - Pf6N2E2_582 0.60 -0.8 658,051 - Pf6N2E2_582 0.60 -2.0 658,144 - Pf6N2E2_582 0.69 +0.9 658,159 - Pf6N2E2_582 0.70 -0.9 658,159 - Pf6N2E2_582 0.70 -1.0 658,159 - Pf6N2E2_582 0.70 +1.4 658,221 + Pf6N2E2_582 0.76 -1.3 658,221 + Pf6N2E2_582 0.76 -2.4 658,222 - Pf6N2E2_582 0.76 -3.1 658,222 - Pf6N2E2_582 0.76 -0.2 658,222 - Pf6N2E2_582 0.76 -0.8 658,222 - Pf6N2E2_582 0.76 +1.6 658,222 - Pf6N2E2_582 0.76 -0.7 658,233 + Pf6N2E2_582 0.77 -2.0 658,233 + Pf6N2E2_582 0.77 +0.7 658,233 + Pf6N2E2_582 0.77 -2.3 658,234 - Pf6N2E2_582 0.78 -2.1 658,314 + Pf6N2E2_582 0.85 +2.1 658,315 - Pf6N2E2_582 0.85 +0.9
Or see this region's nucleotide sequence