Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_543 and Pf6N2E2_544 are separated by 153 nucleotides Pf6N2E2_544 and Pf6N2E2_545 are separated by 24 nucleotides Pf6N2E2_545 and Pf6N2E2_546 are separated by 198 nucleotides Pf6N2E2_546 and Pf6N2E2_547 are separated by 221 nucleotides
Pf6N2E2_543 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 612,214 to 613,329
_543
Pf6N2E2_544 - Short-chain dehydrogenase/reductase SDR, at 613,483 to 614,250
_544
Pf6N2E2_545 - Predicted aminoglycoside phosphotransferase, at 614,275 to 615,342
_545
Pf6N2E2_546 - FIG00966904: hypothetical protein, at 615,541 to 615,936
_546
Pf6N2E2_547 - Phophatidylinositol-4-phosphate 5-kinase (EC 2.7.1.68), at 616,158 to 616,700
_547
Position (kb) 614
615
616 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 613.444 kb on + strand at 613.444 kb on + strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.529 kb on + strand at 613.529 kb on + strand at 613.553 kb on + strand at 613.553 kb on + strand at 613.554 kb on - strand at 613.769 kb on + strand, within Pf6N2E2_544 at 613.769 kb on + strand, within Pf6N2E2_544 at 613.769 kb on + strand, within Pf6N2E2_544 at 613.770 kb on - strand, within Pf6N2E2_544 at 613.773 kb on + strand, within Pf6N2E2_544 at 613.774 kb on - strand, within Pf6N2E2_544 at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.267 kb on - strand at 614.267 kb on - strand at 614.267 kb on - strand at 614.267 kb on - strand at 614.267 kb on - strand at 614.387 kb on + strand, within Pf6N2E2_545 at 614.387 kb on + strand, within Pf6N2E2_545 at 614.387 kb on + strand, within Pf6N2E2_545 at 614.388 kb on - strand, within Pf6N2E2_545 at 614.388 kb on - strand, within Pf6N2E2_545 at 614.388 kb on - strand, within Pf6N2E2_545 at 614.388 kb on - strand, within Pf6N2E2_545 at 614.426 kb on + strand, within Pf6N2E2_545 at 614.426 kb on + strand, within Pf6N2E2_545 at 614.426 kb on + strand, within Pf6N2E2_545 at 614.426 kb on + strand, within Pf6N2E2_545 at 614.427 kb on - strand, within Pf6N2E2_545 at 614.427 kb on - strand, within Pf6N2E2_545 at 614.432 kb on + strand, within Pf6N2E2_545 at 614.432 kb on + strand, within Pf6N2E2_545 at 614.432 kb on + strand, within Pf6N2E2_545 at 614.432 kb on + strand, within Pf6N2E2_545 at 614.433 kb on - strand, within Pf6N2E2_545 at 614.433 kb on - strand, within Pf6N2E2_545 at 614.433 kb on - strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.435 kb on + strand, within Pf6N2E2_545 at 614.436 kb on - strand, within Pf6N2E2_545 at 614.436 kb on - strand, within Pf6N2E2_545 at 614.436 kb on - strand, within Pf6N2E2_545 at 614.436 kb on - strand, within Pf6N2E2_545 at 614.436 kb on - strand, within Pf6N2E2_545 at 614.436 kb on - strand, within Pf6N2E2_545 at 614.436 kb on - strand, within Pf6N2E2_545 at 614.477 kb on + strand, within Pf6N2E2_545 at 614.478 kb on - strand, within Pf6N2E2_545 at 614.481 kb on - strand, within Pf6N2E2_545 at 614.693 kb on + strand, within Pf6N2E2_545 at 615.011 kb on + strand, within Pf6N2E2_545 at 615.011 kb on + strand, within Pf6N2E2_545 at 615.012 kb on - strand, within Pf6N2E2_545 at 615.012 kb on - strand, within Pf6N2E2_545 at 615.012 kb on - strand, within Pf6N2E2_545 at 615.053 kb on + strand, within Pf6N2E2_545 at 615.053 kb on + strand, within Pf6N2E2_545 at 615.194 kb on + strand, within Pf6N2E2_545 at 615.194 kb on + strand, within Pf6N2E2_545 at 615.195 kb on - strand, within Pf6N2E2_545 at 615.195 kb on - strand, within Pf6N2E2_545 at 615.272 kb on + strand at 615.360 kb on - strand at 615.429 kb on + strand at 615.429 kb on + strand at 615.430 kb on - strand at 615.430 kb on - strand at 615.430 kb on - strand at 615.430 kb on - strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.431 kb on + strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.432 kb on - strand at 615.501 kb on + strand at 615.859 kb on - strand, within Pf6N2E2_546 at 615.876 kb on + strand, within Pf6N2E2_546 at 615.877 kb on - strand, within Pf6N2E2_546 at 615.938 kb on + strand at 615.963 kb on + strand at 615.963 kb on + strand at 615.963 kb on + strand at 615.963 kb on + strand at 615.964 kb on - strand at 615.964 kb on - strand at 615.964 kb on - strand at 615.964 kb on - strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.967 kb on + strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.968 kb on - strand at 615.972 kb on + strand at 615.973 kb on - strand at 615.973 kb on - strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.976 kb on + strand at 615.977 kb on - strand at 615.977 kb on - strand at 615.977 kb on - strand at 615.977 kb on - strand at 615.977 kb on - strand at 615.984 kb on + strand at 615.984 kb on + strand at 615.984 kb on + strand at 615.984 kb on + strand at 615.985 kb on - strand at 615.985 kb on - strand at 615.985 kb on - strand at 616.092 kb on + strand at 616.160 kb on - strand at 616.160 kb on - strand at 616.160 kb on - strand at 616.160 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 613,444 + +0.7 613,444 + +0.8 613,445 - +1.8 613,445 - +0.3 613,445 - +3.3 613,445 - +1.4 613,445 - +1.2 613,445 - -0.2 613,529 + +0.7 613,529 + +1.8 613,553 + -0.5 613,553 + -1.4 613,554 - -0.7 613,769 + Pf6N2E2_544 0.37 +1.4 613,769 + Pf6N2E2_544 0.37 +2.4 613,769 + Pf6N2E2_544 0.37 +1.3 613,770 - Pf6N2E2_544 0.37 +0.2 613,773 + Pf6N2E2_544 0.38 +1.5 613,774 - Pf6N2E2_544 0.38 -0.1 614,266 + +1.4 614,266 + -1.2 614,266 + -0.7 614,266 + -2.6 614,266 + -1.2 614,266 + +0.7 614,266 + +0.6 614,267 - -2.3 614,267 - +1.0 614,267 - +0.6 614,267 - +0.9 614,267 - +0.7 614,387 + Pf6N2E2_545 0.10 +1.1 614,387 + Pf6N2E2_545 0.10 +0.2 614,387 + Pf6N2E2_545 0.10 -0.2 614,388 - Pf6N2E2_545 0.11 -1.9 614,388 - Pf6N2E2_545 0.11 -2.0 614,388 - Pf6N2E2_545 0.11 -2.8 614,388 - Pf6N2E2_545 0.11 +0.6 614,426 + Pf6N2E2_545 0.14 +0.6 614,426 + Pf6N2E2_545 0.14 -0.3 614,426 + Pf6N2E2_545 0.14 +1.7 614,426 + Pf6N2E2_545 0.14 +1.8 614,427 - Pf6N2E2_545 0.14 +2.7 614,427 - Pf6N2E2_545 0.14 +0.7 614,432 + Pf6N2E2_545 0.15 +0.0 614,432 + Pf6N2E2_545 0.15 -0.5 614,432 + Pf6N2E2_545 0.15 -2.0 614,432 + Pf6N2E2_545 0.15 +0.1 614,433 - Pf6N2E2_545 0.15 +0.6 614,433 - Pf6N2E2_545 0.15 -1.2 614,433 - Pf6N2E2_545 0.15 -2.1 614,435 + Pf6N2E2_545 0.15 -1.0 614,435 + Pf6N2E2_545 0.15 -0.5 614,435 + Pf6N2E2_545 0.15 -1.6 614,435 + Pf6N2E2_545 0.15 -2.4 614,435 + Pf6N2E2_545 0.15 -2.6 614,435 + Pf6N2E2_545 0.15 +1.0 614,435 + Pf6N2E2_545 0.15 -1.7 614,435 + Pf6N2E2_545 0.15 +2.3 614,435 + Pf6N2E2_545 0.15 -1.0 614,436 - Pf6N2E2_545 0.15 +2.4 614,436 - Pf6N2E2_545 0.15 -1.9 614,436 - Pf6N2E2_545 0.15 -1.4 614,436 - Pf6N2E2_545 0.15 -3.2 614,436 - Pf6N2E2_545 0.15 +0.0 614,436 - Pf6N2E2_545 0.15 -0.4 614,436 - Pf6N2E2_545 0.15 +1.6 614,477 + Pf6N2E2_545 0.19 +0.4 614,478 - Pf6N2E2_545 0.19 -1.1 614,481 - Pf6N2E2_545 0.19 -0.4 614,693 + Pf6N2E2_545 0.39 +0.5 615,011 + Pf6N2E2_545 0.69 -1.7 615,011 + Pf6N2E2_545 0.69 +0.6 615,012 - Pf6N2E2_545 0.69 -2.2 615,012 - Pf6N2E2_545 0.69 -1.2 615,012 - Pf6N2E2_545 0.69 -1.7 615,053 + Pf6N2E2_545 0.73 +0.3 615,053 + Pf6N2E2_545 0.73 +0.2 615,194 + Pf6N2E2_545 0.86 +2.1 615,194 + Pf6N2E2_545 0.86 +0.6 615,195 - Pf6N2E2_545 0.86 -0.2 615,195 - Pf6N2E2_545 0.86 +0.6 615,272 + +1.2 615,360 - +3.2 615,429 + +0.4 615,429 + -3.0 615,430 - -2.6 615,430 - -2.6 615,430 - -0.5 615,430 - -0.5 615,431 + -2.2 615,431 + -1.4 615,431 + +1.6 615,431 + +0.9 615,431 + +0.1 615,431 + -0.2 615,431 + -0.9 615,431 + +0.0 615,431 + +2.0 615,431 + +0.7 615,431 + -0.9 615,432 - -1.6 615,432 - +1.8 615,432 - +2.2 615,432 - -0.6 615,432 - +2.8 615,432 - +0.7 615,432 - -2.3 615,432 - -0.4 615,501 + -1.9 615,859 - Pf6N2E2_546 0.80 +2.9 615,876 + Pf6N2E2_546 0.85 +0.9 615,877 - Pf6N2E2_546 0.85 +1.6 615,938 + -0.3 615,963 + -1.0 615,963 + -1.2 615,963 + -0.6 615,963 + +0.4 615,964 - +0.6 615,964 - +0.1 615,964 - -1.2 615,964 - -0.5 615,967 + -1.4 615,967 + +1.0 615,967 + +1.6 615,967 + +0.5 615,967 + +0.4 615,967 + -0.8 615,967 + +0.9 615,968 - +1.4 615,968 - +0.4 615,968 - -1.6 615,968 - +0.6 615,968 - +1.7 615,968 - -2.3 615,968 - -3.0 615,968 - +1.8 615,968 - -1.5 615,968 - -1.2 615,968 - -2.4 615,968 - -1.2 615,968 - +0.7 615,972 + -1.3 615,973 - -0.7 615,973 - -2.1 615,976 + -0.6 615,976 + +2.2 615,976 + -0.7 615,976 + +1.4 615,976 + +0.0 615,976 + +0.6 615,976 + +1.2 615,976 + +1.8 615,976 + +1.1 615,976 + -0.9 615,977 - +0.8 615,977 - -0.4 615,977 - +1.3 615,977 - +1.3 615,977 - -1.4 615,984 + -1.9 615,984 + +0.2 615,984 + -1.4 615,984 + +1.3 615,985 - +0.2 615,985 - +2.5 615,985 - +0.9 616,092 + -0.7 616,160 - -0.5 616,160 - -0.3 616,160 - +0.3 616,160 - +4.3
Or see this region's nucleotide sequence