Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5223 and Pf6N2E2_5224 overlap by 8 nucleotides Pf6N2E2_5224 and Pf6N2E2_5225 are separated by 114 nucleotides Pf6N2E2_5225 and Pf6N2E2_5226 are separated by 59 nucleotides Pf6N2E2_5226 and Pf6N2E2_5227 are separated by 27 nucleotides
Pf6N2E2_5223 - Molybdopterin biosynthesis protein MoeB, at 6,021,840 to 6,022,595
_5223
Pf6N2E2_5224 - Glutamate racemase (EC 5.1.1.3), at 6,022,588 to 6,023,379
_5224
Pf6N2E2_5225 - Lipid A 3-O-deacylase, at 6,023,494 to 6,024,012
_5225
Pf6N2E2_5226 - FIG00955589: hypothetical protein, at 6,024,072 to 6,024,377
_5226
Pf6N2E2_5227 - Oxidoreductase, short-chain dehydrogenase/reductase family (EC 1.1.1.-), at 6,024,405 to 6,025,178
_5227
Position (kb)
6023
6024
6025 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6023.334 kb on + strand at 6023.334 kb on + strand at 6023.334 kb on + strand at 6023.339 kb on + strand at 6023.436 kb on - strand at 6023.437 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.467 kb on - strand at 6023.467 kb on - strand at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.549 kb on - strand, within Pf6N2E2_5225 at 6023.549 kb on - strand, within Pf6N2E2_5225 at 6023.549 kb on - strand, within Pf6N2E2_5225 at 6023.611 kb on + strand, within Pf6N2E2_5225 at 6023.612 kb on - strand, within Pf6N2E2_5225 at 6023.695 kb on + strand, within Pf6N2E2_5225 at 6023.695 kb on + strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.696 kb on - strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.697 kb on + strand, within Pf6N2E2_5225 at 6023.698 kb on - strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.701 kb on + strand, within Pf6N2E2_5225 at 6023.702 kb on - strand, within Pf6N2E2_5225 at 6023.702 kb on - strand, within Pf6N2E2_5225 at 6023.702 kb on - strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.791 kb on + strand, within Pf6N2E2_5225 at 6023.792 kb on - strand, within Pf6N2E2_5225 at 6023.792 kb on - strand, within Pf6N2E2_5225 at 6023.792 kb on - strand, within Pf6N2E2_5225 at 6023.792 kb on - strand, within Pf6N2E2_5225 at 6023.938 kb on + strand, within Pf6N2E2_5225 at 6023.938 kb on + strand, within Pf6N2E2_5225 at 6023.975 kb on + strand at 6023.975 kb on + strand at 6023.975 kb on + strand at 6023.975 kb on + strand at 6023.976 kb on - strand at 6023.976 kb on - strand at 6024.008 kb on + strand at 6024.011 kb on - strand at 6024.011 kb on - strand at 6024.055 kb on + strand at 6024.055 kb on + strand at 6024.055 kb on + strand at 6024.056 kb on - strand at 6024.056 kb on - strand at 6024.056 kb on - strand at 6024.056 kb on - strand at 6024.164 kb on - strand, within Pf6N2E2_5226 at 6024.164 kb on - strand, within Pf6N2E2_5226 at 6024.389 kb on + strand at 6024.390 kb on - strand at 6024.390 kb on - strand at 6024.390 kb on - strand at 6024.390 kb on - strand at 6024.390 kb on - strand at 6024.638 kb on - strand, within Pf6N2E2_5227 at 6024.648 kb on + strand, within Pf6N2E2_5227 at 6024.751 kb on + strand, within Pf6N2E2_5227 at 6024.752 kb on - strand, within Pf6N2E2_5227 at 6024.771 kb on - strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.861 kb on - strand, within Pf6N2E2_5227 at 6024.861 kb on - strand, within Pf6N2E2_5227 at 6024.861 kb on - strand, within Pf6N2E2_5227 at 6024.861 kb on - strand, within Pf6N2E2_5227 at 6024.901 kb on + strand, within Pf6N2E2_5227 at 6024.901 kb on + strand, within Pf6N2E2_5227 at 6024.901 kb on + strand, within Pf6N2E2_5227 at 6024.902 kb on - strand, within Pf6N2E2_5227
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 6,023,334 + -2.3 6,023,334 + -2.5 6,023,334 + -3.4 6,023,339 + -1.0 6,023,436 - -2.0 6,023,437 + -1.5 6,023,466 + -2.4 6,023,466 + -0.1 6,023,466 + -2.3 6,023,466 + -0.2 6,023,466 + -0.6 6,023,467 - +0.3 6,023,467 - +0.7 6,023,548 + Pf6N2E2_5225 0.10 +0.3 6,023,548 + Pf6N2E2_5225 0.10 -2.9 6,023,548 + Pf6N2E2_5225 0.10 -1.0 6,023,548 + Pf6N2E2_5225 0.10 +0.3 6,023,548 + Pf6N2E2_5225 0.10 -1.2 6,023,548 + Pf6N2E2_5225 0.10 -1.3 6,023,548 + Pf6N2E2_5225 0.10 -2.3 6,023,548 + Pf6N2E2_5225 0.10 -2.0 6,023,548 + Pf6N2E2_5225 0.10 +0.7 6,023,549 - Pf6N2E2_5225 0.11 -2.9 6,023,549 - Pf6N2E2_5225 0.11 -2.3 6,023,549 - Pf6N2E2_5225 0.11 -1.0 6,023,611 + Pf6N2E2_5225 0.23 -1.5 6,023,612 - Pf6N2E2_5225 0.23 -2.5 6,023,695 + Pf6N2E2_5225 0.39 -1.4 6,023,695 + Pf6N2E2_5225 0.39 +0.2 6,023,696 - Pf6N2E2_5225 0.39 -0.2 6,023,696 - Pf6N2E2_5225 0.39 +0.8 6,023,696 - Pf6N2E2_5225 0.39 -0.2 6,023,696 - Pf6N2E2_5225 0.39 -1.8 6,023,696 - Pf6N2E2_5225 0.39 -3.6 6,023,697 + Pf6N2E2_5225 0.39 -0.7 6,023,697 + Pf6N2E2_5225 0.39 -3.0 6,023,697 + Pf6N2E2_5225 0.39 -3.0 6,023,697 + Pf6N2E2_5225 0.39 -2.8 6,023,697 + Pf6N2E2_5225 0.39 -0.0 6,023,697 + Pf6N2E2_5225 0.39 -1.5 6,023,697 + Pf6N2E2_5225 0.39 -1.5 6,023,697 + Pf6N2E2_5225 0.39 -1.2 6,023,698 - Pf6N2E2_5225 0.39 -1.4 6,023,701 + Pf6N2E2_5225 0.40 +0.5 6,023,701 + Pf6N2E2_5225 0.40 -2.7 6,023,701 + Pf6N2E2_5225 0.40 -0.2 6,023,701 + Pf6N2E2_5225 0.40 -1.8 6,023,701 + Pf6N2E2_5225 0.40 -1.6 6,023,702 - Pf6N2E2_5225 0.40 +0.5 6,023,702 - Pf6N2E2_5225 0.40 -0.3 6,023,702 - Pf6N2E2_5225 0.40 -2.4 6,023,791 + Pf6N2E2_5225 0.57 -1.7 6,023,791 + Pf6N2E2_5225 0.57 -0.6 6,023,791 + Pf6N2E2_5225 0.57 -0.9 6,023,791 + Pf6N2E2_5225 0.57 -2.4 6,023,791 + Pf6N2E2_5225 0.57 -2.0 6,023,791 + Pf6N2E2_5225 0.57 -1.0 6,023,791 + Pf6N2E2_5225 0.57 -1.1 6,023,792 - Pf6N2E2_5225 0.57 -1.9 6,023,792 - Pf6N2E2_5225 0.57 -0.6 6,023,792 - Pf6N2E2_5225 0.57 -0.8 6,023,792 - Pf6N2E2_5225 0.57 -0.0 6,023,938 + Pf6N2E2_5225 0.86 +1.3 6,023,938 + Pf6N2E2_5225 0.86 +0.0 6,023,975 + +0.1 6,023,975 + -0.7 6,023,975 + -1.5 6,023,975 + -0.9 6,023,976 - -0.1 6,023,976 - -1.2 6,024,008 + -2.3 6,024,011 - -0.3 6,024,011 - +0.1 6,024,055 + -0.4 6,024,055 + +1.0 6,024,055 + +0.6 6,024,056 - -0.7 6,024,056 - -4.3 6,024,056 - +0.7 6,024,056 - +0.4 6,024,164 - Pf6N2E2_5226 0.30 +0.2 6,024,164 - Pf6N2E2_5226 0.30 +1.5 6,024,389 + -1.3 6,024,390 - -1.4 6,024,390 - +1.1 6,024,390 - -2.1 6,024,390 - -1.0 6,024,390 - -0.2 6,024,638 - Pf6N2E2_5227 0.30 +1.0 6,024,648 + Pf6N2E2_5227 0.31 +1.3 6,024,751 + Pf6N2E2_5227 0.45 +0.9 6,024,752 - Pf6N2E2_5227 0.45 +2.0 6,024,771 - Pf6N2E2_5227 0.47 +1.1 6,024,860 + Pf6N2E2_5227 0.59 +0.0 6,024,860 + Pf6N2E2_5227 0.59 -2.1 6,024,860 + Pf6N2E2_5227 0.59 +0.5 6,024,860 + Pf6N2E2_5227 0.59 -0.4 6,024,860 + Pf6N2E2_5227 0.59 -0.4 6,024,860 + Pf6N2E2_5227 0.59 -0.1 6,024,861 - Pf6N2E2_5227 0.59 +1.5 6,024,861 - Pf6N2E2_5227 0.59 -0.6 6,024,861 - Pf6N2E2_5227 0.59 -2.3 6,024,861 - Pf6N2E2_5227 0.59 -2.2 6,024,901 + Pf6N2E2_5227 0.64 -3.5 6,024,901 + Pf6N2E2_5227 0.64 -0.0 6,024,901 + Pf6N2E2_5227 0.64 -2.5 6,024,902 - Pf6N2E2_5227 0.64 +0.8
Or see this region's nucleotide sequence