Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_498 and Pf6N2E2_499 are separated by 608 nucleotides Pf6N2E2_499 and Pf6N2E2_500 are separated by 78 nucleotides
Pf6N2E2_498 - Dihydropyrimidinase (EC 3.5.2.2) <small>(from data)</small>, at 565,573 to 567,012
_498
Pf6N2E2_499 - Possible pyrimidine permease in reductive pathway, at 567,621 to 569,099
_499
Pf6N2E2_500 - Beta-ureidopropionase (EC 3.5.1.6), at 569,178 to 570,461
_500
Position (kb) 567
568
569
570 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 566.829 kb on + strand, within Pf6N2E2_498 at 566.829 kb on + strand, within Pf6N2E2_498 at 566.829 kb on + strand, within Pf6N2E2_498 at 566.830 kb on - strand, within Pf6N2E2_498 at 566.830 kb on - strand, within Pf6N2E2_498 at 566.864 kb on + strand, within Pf6N2E2_498 at 566.864 kb on + strand, within Pf6N2E2_498 at 566.864 kb on + strand, within Pf6N2E2_498 at 566.865 kb on - strand, within Pf6N2E2_498 at 566.979 kb on + strand at 567.061 kb on + strand at 567.062 kb on - strand at 567.077 kb on + strand at 567.095 kb on + strand at 567.096 kb on - strand at 567.181 kb on - strand at 567.288 kb on + strand at 567.392 kb on - strand at 567.406 kb on + strand at 567.473 kb on + strand at 567.473 kb on + strand at 567.475 kb on + strand at 567.567 kb on + strand at 567.567 kb on + strand at 567.701 kb on + strand at 567.701 kb on + strand at 567.701 kb on + strand at 567.702 kb on - strand at 567.798 kb on + strand, within Pf6N2E2_499 at 567.798 kb on + strand, within Pf6N2E2_499 at 567.799 kb on - strand, within Pf6N2E2_499 at 567.799 kb on - strand, within Pf6N2E2_499 at 568.317 kb on + strand, within Pf6N2E2_499 at 568.317 kb on + strand, within Pf6N2E2_499 at 568.318 kb on - strand, within Pf6N2E2_499 at 568.318 kb on - strand, within Pf6N2E2_499 at 568.318 kb on - strand, within Pf6N2E2_499 at 568.783 kb on - strand, within Pf6N2E2_499 at 568.848 kb on + strand, within Pf6N2E2_499 at 568.849 kb on - strand, within Pf6N2E2_499 at 568.905 kb on + strand, within Pf6N2E2_499 at 568.998 kb on + strand at 568.998 kb on + strand at 568.999 kb on - strand at 569.043 kb on + strand at 569.200 kb on + strand at 569.200 kb on + strand at 569.201 kb on - strand at 569.760 kb on + strand, within Pf6N2E2_500 at 569.761 kb on - strand, within Pf6N2E2_500 at 569.909 kb on + strand, within Pf6N2E2_500 at 569.910 kb on - strand, within Pf6N2E2_500
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 566,829 + Pf6N2E2_498 0.87 -1.2 566,829 + Pf6N2E2_498 0.87 +1.5 566,829 + Pf6N2E2_498 0.87 -2.3 566,830 - Pf6N2E2_498 0.87 -0.4 566,830 - Pf6N2E2_498 0.87 -0.2 566,864 + Pf6N2E2_498 0.90 -0.4 566,864 + Pf6N2E2_498 0.90 +0.6 566,864 + Pf6N2E2_498 0.90 -1.7 566,865 - Pf6N2E2_498 0.90 +0.8 566,979 + +1.3 567,061 + -0.7 567,062 - -1.6 567,077 + -0.8 567,095 + -1.7 567,096 - +0.1 567,181 - -2.1 567,288 + -2.8 567,392 - +0.6 567,406 + -0.5 567,473 + +1.2 567,473 + +0.3 567,475 + -0.4 567,567 + -1.9 567,567 + +1.0 567,701 + +1.6 567,701 + +2.3 567,701 + -0.8 567,702 - +0.6 567,798 + Pf6N2E2_499 0.12 -1.5 567,798 + Pf6N2E2_499 0.12 -0.8 567,799 - Pf6N2E2_499 0.12 -2.8 567,799 - Pf6N2E2_499 0.12 -1.4 568,317 + Pf6N2E2_499 0.47 +1.9 568,317 + Pf6N2E2_499 0.47 -2.4 568,318 - Pf6N2E2_499 0.47 +0.4 568,318 - Pf6N2E2_499 0.47 +1.9 568,318 - Pf6N2E2_499 0.47 -3.1 568,783 - Pf6N2E2_499 0.79 -0.9 568,848 + Pf6N2E2_499 0.83 -0.8 568,849 - Pf6N2E2_499 0.83 -1.4 568,905 + Pf6N2E2_499 0.87 -2.5 568,998 + +2.2 568,998 + +1.5 568,999 - +1.6 569,043 + -2.6 569,200 + -0.8 569,200 + -1.5 569,201 - -1.1 569,760 + Pf6N2E2_500 0.45 +0.9 569,761 - Pf6N2E2_500 0.45 +0.8 569,909 + Pf6N2E2_500 0.57 +0.4 569,910 - Pf6N2E2_500 0.57 -0.2
Or see this region's nucleotide sequence