Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4691 and Pf6N2E2_4692 are separated by 38 nucleotides Pf6N2E2_4692 and Pf6N2E2_4693 are separated by 71 nucleotides Pf6N2E2_4693 and Pf6N2E2_4694 are separated by 186 nucleotides
Pf6N2E2_4691 - hypothetical protein, at 5,424,942 to 5,425,070
_4691
Pf6N2E2_4692 - dehydrocarnitine cleavage enzyme (EC 2.3.1.-) (from data) , at 5,425,109 to 5,425,996
_4692
Pf6N2E2_4693 - ABC transporter for Carnitine Hydrochloride, periplasmic substrate-binding component (from data) , at 5,426,068 to 5,427,012
_4693
Pf6N2E2_4694 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 5,427,199 to 5,428,143
_4694
Position (kb)
5426
5427
5428 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5425.099 kb on + strand at 5425.099 kb on + strand at 5425.100 kb on - strand at 5425.100 kb on - strand at 5425.100 kb on - strand at 5425.100 kb on - strand at 5425.226 kb on + strand, within Pf6N2E2_4692 at 5425.226 kb on + strand, within Pf6N2E2_4692 at 5425.226 kb on + strand, within Pf6N2E2_4692 at 5425.227 kb on - strand, within Pf6N2E2_4692 at 5425.227 kb on - strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.573 kb on - strand, within Pf6N2E2_4692 at 5425.573 kb on - strand, within Pf6N2E2_4692 at 5425.573 kb on - strand, within Pf6N2E2_4692 at 5425.573 kb on - strand, within Pf6N2E2_4692 at 5425.573 kb on - strand, within Pf6N2E2_4692 at 5425.573 kb on - strand, within Pf6N2E2_4692 at 5425.573 kb on - strand, within Pf6N2E2_4692 at 5425.804 kb on - strand, within Pf6N2E2_4692 at 5426.020 kb on + strand at 5426.020 kb on + strand at 5426.020 kb on + strand at 5426.021 kb on - strand at 5426.073 kb on + strand at 5426.118 kb on + strand at 5426.118 kb on + strand at 5426.118 kb on + strand at 5426.118 kb on + strand at 5426.119 kb on - strand at 5426.119 kb on - strand at 5426.366 kb on + strand, within Pf6N2E2_4693 at 5426.366 kb on + strand, within Pf6N2E2_4693 at 5426.367 kb on - strand, within Pf6N2E2_4693 at 5426.570 kb on + strand, within Pf6N2E2_4693 at 5426.570 kb on + strand, within Pf6N2E2_4693 at 5426.570 kb on + strand, within Pf6N2E2_4693 at 5426.570 kb on + strand, within Pf6N2E2_4693 at 5426.570 kb on + strand, within Pf6N2E2_4693 at 5426.571 kb on - strand, within Pf6N2E2_4693 at 5426.571 kb on - strand, within Pf6N2E2_4693 at 5426.642 kb on + strand, within Pf6N2E2_4693 at 5426.642 kb on + strand, within Pf6N2E2_4693 at 5426.642 kb on + strand, within Pf6N2E2_4693 at 5426.643 kb on - strand, within Pf6N2E2_4693 at 5426.837 kb on + strand, within Pf6N2E2_4693 at 5426.986 kb on + strand at 5426.986 kb on + strand at 5426.987 kb on - strand at 5426.987 kb on - strand at 5426.987 kb on - strand at 5426.987 kb on - strand at 5427.032 kb on + strand at 5427.032 kb on + strand at 5427.032 kb on + strand at 5427.032 kb on + strand at 5427.033 kb on - strand at 5427.033 kb on - strand at 5427.076 kb on - strand at 5427.160 kb on - strand at 5427.657 kb on + strand, within Pf6N2E2_4694 at 5427.657 kb on + strand, within Pf6N2E2_4694 at 5427.658 kb on - strand, within Pf6N2E2_4694 at 5427.812 kb on - strand, within Pf6N2E2_4694 at 5427.973 kb on + strand, within Pf6N2E2_4694 at 5427.973 kb on + strand, within Pf6N2E2_4694 at 5427.974 kb on - strand, within Pf6N2E2_4694
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 5,425,099 + -2.4 5,425,099 + -0.4 5,425,100 - -1.7 5,425,100 - -0.7 5,425,100 - +0.7 5,425,100 - -0.4 5,425,226 + Pf6N2E2_4692 0.13 -0.6 5,425,226 + Pf6N2E2_4692 0.13 -2.1 5,425,226 + Pf6N2E2_4692 0.13 +0.1 5,425,227 - Pf6N2E2_4692 0.13 -1.8 5,425,227 - Pf6N2E2_4692 0.13 +0.6 5,425,572 + Pf6N2E2_4692 0.52 +1.4 5,425,572 + Pf6N2E2_4692 0.52 -1.8 5,425,572 + Pf6N2E2_4692 0.52 +0.5 5,425,572 + Pf6N2E2_4692 0.52 +1.2 5,425,572 + Pf6N2E2_4692 0.52 +1.6 5,425,572 + Pf6N2E2_4692 0.52 -1.8 5,425,572 + Pf6N2E2_4692 0.52 -3.4 5,425,572 + Pf6N2E2_4692 0.52 +0.2 5,425,572 + Pf6N2E2_4692 0.52 +2.8 5,425,573 - Pf6N2E2_4692 0.52 -0.8 5,425,573 - Pf6N2E2_4692 0.52 -0.8 5,425,573 - Pf6N2E2_4692 0.52 -4.3 5,425,573 - Pf6N2E2_4692 0.52 +0.3 5,425,573 - Pf6N2E2_4692 0.52 -2.9 5,425,573 - Pf6N2E2_4692 0.52 -2.9 5,425,573 - Pf6N2E2_4692 0.52 -2.2 5,425,804 - Pf6N2E2_4692 0.78 -0.7 5,426,020 + -1.1 5,426,020 + +0.8 5,426,020 + +0.8 5,426,021 - +0.3 5,426,073 + +2.1 5,426,118 + -2.6 5,426,118 + +1.5 5,426,118 + -0.5 5,426,118 + -0.6 5,426,119 - +1.3 5,426,119 - -1.2 5,426,366 + Pf6N2E2_4693 0.32 +1.8 5,426,366 + Pf6N2E2_4693 0.32 -2.4 5,426,367 - Pf6N2E2_4693 0.32 -1.2 5,426,570 + Pf6N2E2_4693 0.53 -0.8 5,426,570 + Pf6N2E2_4693 0.53 -2.2 5,426,570 + Pf6N2E2_4693 0.53 -2.0 5,426,570 + Pf6N2E2_4693 0.53 -1.2 5,426,570 + Pf6N2E2_4693 0.53 -0.6 5,426,571 - Pf6N2E2_4693 0.53 -0.5 5,426,571 - Pf6N2E2_4693 0.53 +0.1 5,426,642 + Pf6N2E2_4693 0.61 -0.2 5,426,642 + Pf6N2E2_4693 0.61 +1.7 5,426,642 + Pf6N2E2_4693 0.61 +1.8 5,426,643 - Pf6N2E2_4693 0.61 -0.8 5,426,837 + Pf6N2E2_4693 0.81 -0.4 5,426,986 + -2.8 5,426,986 + -1.7 5,426,987 - -2.0 5,426,987 - -1.8 5,426,987 - +0.1 5,426,987 - +2.3 5,427,032 + -1.9 5,427,032 + -2.7 5,427,032 + -0.1 5,427,032 + -2.0 5,427,033 - -3.0 5,427,033 - +1.7 5,427,076 - -2.2 5,427,160 - -2.5 5,427,657 + Pf6N2E2_4694 0.48 -2.8 5,427,657 + Pf6N2E2_4694 0.48 +0.9 5,427,658 - Pf6N2E2_4694 0.49 -2.1 5,427,812 - Pf6N2E2_4694 0.65 -0.2 5,427,973 + Pf6N2E2_4694 0.82 +1.4 5,427,973 + Pf6N2E2_4694 0.82 -2.0 5,427,974 - Pf6N2E2_4694 0.82 +0.8
Or see this region's nucleotide sequence