Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_464 and Pf6N2E2_465 are separated by 68 nucleotides Pf6N2E2_465 and Pf6N2E2_466 are separated by 41 nucleotides Pf6N2E2_466 and Pf6N2E2_467 are separated by 296 nucleotides
Pf6N2E2_464 - Sensory histidine kinase QseC, at 529,360 to 530,661
_464
Pf6N2E2_465 - Probable transmembrane protein, at 530,730 to 531,371
_465
Pf6N2E2_466 - Nicotinamidase (EC 3.5.1.19), at 531,413 to 532,066
_466
Pf6N2E2_467 - Sensory box/GGDEF family protein, at 532,363 to 534,441
_467
Position (kb) 531
532
533 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 530.732 kb on - strand at 530.754 kb on - strand at 530.890 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.906 kb on - strand, within Pf6N2E2_465 at 530.906 kb on - strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.966 kb on - strand, within Pf6N2E2_465 at 530.968 kb on + strand, within Pf6N2E2_465 at 530.968 kb on + strand, within Pf6N2E2_465 at 530.969 kb on - strand, within Pf6N2E2_465 at 530.969 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.404 kb on + strand at 531.404 kb on + strand at 531.405 kb on - strand at 531.405 kb on - strand at 531.434 kb on + strand at 531.435 kb on - strand at 531.435 kb on - strand at 531.615 kb on + strand, within Pf6N2E2_466 at 531.615 kb on + strand, within Pf6N2E2_466 at 531.616 kb on - strand, within Pf6N2E2_466 at 531.696 kb on + strand, within Pf6N2E2_466 at 531.697 kb on - strand, within Pf6N2E2_466 at 531.697 kb on - strand, within Pf6N2E2_466 at 531.792 kb on - strand, within Pf6N2E2_466 at 531.792 kb on - strand, within Pf6N2E2_466 at 531.792 kb on - strand, within Pf6N2E2_466 at 532.014 kb on - strand at 532.014 kb on - strand at 532.100 kb on + strand at 532.101 kb on - strand at 532.101 kb on - strand at 532.126 kb on + strand at 532.127 kb on - strand at 532.127 kb on - strand at 532.200 kb on - strand at 532.244 kb on + strand at 532.244 kb on + strand at 532.297 kb on + strand at 532.297 kb on + strand at 532.297 kb on + strand at 532.297 kb on + strand at 532.297 kb on + strand at 532.298 kb on - strand at 532.302 kb on - strand at 532.302 kb on - strand at 532.349 kb on + strand at 532.349 kb on + strand at 532.378 kb on + strand at 532.378 kb on + strand at 532.379 kb on - strand at 532.429 kb on + strand at 532.429 kb on + strand at 532.429 kb on + strand at 532.429 kb on + strand at 532.430 kb on - strand at 532.430 kb on - strand at 532.430 kb on - strand at 532.589 kb on + strand, within Pf6N2E2_467 at 532.589 kb on + strand, within Pf6N2E2_467 at 532.590 kb on - strand, within Pf6N2E2_467 at 532.590 kb on - strand, within Pf6N2E2_467 at 532.590 kb on - strand, within Pf6N2E2_467 at 532.590 kb on - strand, within Pf6N2E2_467 at 532.729 kb on + strand, within Pf6N2E2_467 at 532.729 kb on + strand, within Pf6N2E2_467 at 532.730 kb on - strand, within Pf6N2E2_467 at 532.730 kb on - strand, within Pf6N2E2_467 at 532.772 kb on - strand, within Pf6N2E2_467 at 532.930 kb on + strand, within Pf6N2E2_467 at 532.930 kb on + strand, within Pf6N2E2_467 at 532.930 kb on + strand, within Pf6N2E2_467 at 532.931 kb on - strand, within Pf6N2E2_467 at 532.931 kb on - strand, within Pf6N2E2_467
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 530,732 - -1.4 530,754 - -1.2 530,890 + Pf6N2E2_465 0.25 +0.9 530,905 + Pf6N2E2_465 0.27 +1.6 530,905 + Pf6N2E2_465 0.27 -1.8 530,905 + Pf6N2E2_465 0.27 +1.2 530,906 - Pf6N2E2_465 0.27 -0.4 530,906 - Pf6N2E2_465 0.27 +0.0 530,965 + Pf6N2E2_465 0.37 +0.7 530,965 + Pf6N2E2_465 0.37 +1.3 530,965 + Pf6N2E2_465 0.37 -1.2 530,965 + Pf6N2E2_465 0.37 -1.8 530,966 - Pf6N2E2_465 0.37 -2.0 530,968 + Pf6N2E2_465 0.37 +0.8 530,968 + Pf6N2E2_465 0.37 +1.3 530,969 - Pf6N2E2_465 0.37 +2.0 530,969 - Pf6N2E2_465 0.37 -2.6 531,239 - Pf6N2E2_465 0.79 -2.6 531,239 - Pf6N2E2_465 0.79 -0.3 531,239 - Pf6N2E2_465 0.79 -1.5 531,401 + -0.3 531,401 + +2.5 531,401 + -1.6 531,401 + -2.8 531,401 + -1.0 531,404 + +0.9 531,404 + -2.2 531,405 - +0.7 531,405 - +2.0 531,434 + -0.4 531,435 - -1.8 531,435 - +2.0 531,615 + Pf6N2E2_466 0.31 +3.1 531,615 + Pf6N2E2_466 0.31 +1.5 531,616 - Pf6N2E2_466 0.31 +2.6 531,696 + Pf6N2E2_466 0.43 +0.6 531,697 - Pf6N2E2_466 0.43 -0.6 531,697 - Pf6N2E2_466 0.43 -1.7 531,792 - Pf6N2E2_466 0.58 +2.8 531,792 - Pf6N2E2_466 0.58 +1.2 531,792 - Pf6N2E2_466 0.58 -2.4 532,014 - -2.6 532,014 - -2.6 532,100 + -0.4 532,101 - -0.4 532,101 - -3.2 532,126 + +0.8 532,127 - -0.0 532,127 - -1.6 532,200 - -1.8 532,244 + +0.5 532,244 + +0.6 532,297 + -1.6 532,297 + +1.3 532,297 + +1.1 532,297 + +1.1 532,297 + +2.4 532,298 - +0.7 532,302 - -2.0 532,302 - -0.2 532,349 + -0.8 532,349 + +0.9 532,378 + +1.9 532,378 + -1.7 532,379 - +1.7 532,429 + -3.2 532,429 + +0.2 532,429 + -0.4 532,429 + +1.5 532,430 - -1.8 532,430 - -2.6 532,430 - -2.3 532,589 + Pf6N2E2_467 0.11 -2.1 532,589 + Pf6N2E2_467 0.11 -2.5 532,590 - Pf6N2E2_467 0.11 -1.0 532,590 - Pf6N2E2_467 0.11 -2.3 532,590 - Pf6N2E2_467 0.11 -1.8 532,590 - Pf6N2E2_467 0.11 +1.1 532,729 + Pf6N2E2_467 0.18 +0.8 532,729 + Pf6N2E2_467 0.18 -1.9 532,730 - Pf6N2E2_467 0.18 +0.3 532,730 - Pf6N2E2_467 0.18 +2.2 532,772 - Pf6N2E2_467 0.20 +1.0 532,930 + Pf6N2E2_467 0.27 -2.7 532,930 + Pf6N2E2_467 0.27 +1.3 532,930 + Pf6N2E2_467 0.27 +1.7 532,931 - Pf6N2E2_467 0.27 -0.7 532,931 - Pf6N2E2_467 0.27 -2.3
Or see this region's nucleotide sequence