Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4406 and Pf6N2E2_4407 overlap by 4 nucleotides Pf6N2E2_4407 and Pf6N2E2_4408 are separated by 3 nucleotides Pf6N2E2_4408 and Pf6N2E2_4409 are separated by 417 nucleotides
Pf6N2E2_4406 - 2-Keto-D-gluconate dehydrogenase (EC 1.1.99.4), membrane-bound, flavoprotein, at 5,139,480 to 5,141,114
_4406
Pf6N2E2_4407 - 2-Keto-D-gluconate dehydrogenase (EC 1.1.99.4), membrane-bound, gamma subunit, at 5,141,111 to 5,141,659
_4407
Pf6N2E2_4408 - 2-Keto-D-gluconate dehydrogenase (EC 1.1.99.4), membrane-bound, cytochrome c, at 5,141,663 to 5,143,087
_4408
Pf6N2E2_4409 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 5,143,505 to 5,145,187
_4409
Position (kb)
5141
5142
5143
5144 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5140.685 kb on - strand, within Pf6N2E2_4406 at 5140.685 kb on - strand, within Pf6N2E2_4406 at 5140.685 kb on - strand, within Pf6N2E2_4406 at 5140.705 kb on + strand, within Pf6N2E2_4406 at 5140.705 kb on + strand, within Pf6N2E2_4406 at 5140.705 kb on + strand, within Pf6N2E2_4406 at 5140.705 kb on + strand, within Pf6N2E2_4406 at 5140.705 kb on + strand, within Pf6N2E2_4406 at 5140.706 kb on - strand, within Pf6N2E2_4406 at 5140.706 kb on - strand, within Pf6N2E2_4406 at 5140.823 kb on - strand, within Pf6N2E2_4406 at 5140.834 kb on + strand, within Pf6N2E2_4406 at 5140.834 kb on + strand, within Pf6N2E2_4406 at 5140.835 kb on - strand at 5140.861 kb on + strand, within Pf6N2E2_4406 at 5140.870 kb on + strand, within Pf6N2E2_4406 at 5140.870 kb on + strand, within Pf6N2E2_4406 at 5140.870 kb on + strand, within Pf6N2E2_4406 at 5140.870 kb on + strand, within Pf6N2E2_4406 at 5140.871 kb on - strand, within Pf6N2E2_4406 at 5140.871 kb on - strand, within Pf6N2E2_4406 at 5140.871 kb on - strand, within Pf6N2E2_4406 at 5140.871 kb on - strand, within Pf6N2E2_4406 at 5141.098 kb on + strand at 5141.098 kb on + strand at 5141.098 kb on + strand at 5141.098 kb on + strand at 5141.099 kb on - strand at 5141.099 kb on - strand at 5141.099 kb on - strand at 5141.114 kb on + strand at 5141.114 kb on + strand at 5141.115 kb on - strand at 5141.115 kb on - strand at 5141.115 kb on - strand at 5141.115 kb on - strand at 5141.172 kb on + strand, within Pf6N2E2_4407 at 5141.172 kb on + strand, within Pf6N2E2_4407 at 5141.172 kb on + strand, within Pf6N2E2_4407 at 5141.208 kb on + strand, within Pf6N2E2_4407 at 5141.208 kb on + strand, within Pf6N2E2_4407 at 5141.209 kb on - strand, within Pf6N2E2_4407 at 5141.209 kb on - strand, within Pf6N2E2_4407 at 5141.272 kb on - strand, within Pf6N2E2_4407 at 5141.272 kb on - strand, within Pf6N2E2_4407 at 5141.337 kb on + strand, within Pf6N2E2_4407 at 5141.337 kb on + strand, within Pf6N2E2_4407 at 5141.337 kb on + strand, within Pf6N2E2_4407 at 5141.338 kb on - strand, within Pf6N2E2_4407 at 5141.338 kb on - strand, within Pf6N2E2_4407 at 5141.433 kb on + strand, within Pf6N2E2_4407 at 5141.437 kb on - strand, within Pf6N2E2_4407 at 5141.612 kb on - strand at 5141.700 kb on + strand at 5141.700 kb on + strand at 5141.701 kb on - strand at 5141.701 kb on - strand at 5141.751 kb on + strand at 5141.751 kb on + strand at 5141.795 kb on + strand at 5141.796 kb on - strand at 5141.856 kb on + strand, within Pf6N2E2_4408 at 5141.856 kb on + strand, within Pf6N2E2_4408 at 5141.856 kb on + strand, within Pf6N2E2_4408 at 5141.856 kb on + strand, within Pf6N2E2_4408 at 5141.856 kb on + strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.967 kb on + strand, within Pf6N2E2_4408 at 5141.968 kb on - strand, within Pf6N2E2_4408 at 5142.247 kb on - strand, within Pf6N2E2_4408 at 5142.247 kb on - strand, within Pf6N2E2_4408 at 5142.247 kb on - strand, within Pf6N2E2_4408 at 5142.394 kb on - strand, within Pf6N2E2_4408 at 5142.501 kb on + strand, within Pf6N2E2_4408 at 5142.502 kb on - strand, within Pf6N2E2_4408 at 5142.502 kb on - strand, within Pf6N2E2_4408 at 5142.502 kb on - strand, within Pf6N2E2_4408 at 5142.666 kb on + strand, within Pf6N2E2_4408 at 5142.666 kb on + strand, within Pf6N2E2_4408 at 5142.666 kb on + strand, within Pf6N2E2_4408 at 5142.667 kb on - strand, within Pf6N2E2_4408 at 5142.667 kb on - strand, within Pf6N2E2_4408 at 5142.667 kb on - strand, within Pf6N2E2_4408 at 5142.667 kb on - strand, within Pf6N2E2_4408 at 5142.667 kb on - strand, within Pf6N2E2_4408 at 5142.672 kb on + strand, within Pf6N2E2_4408 at 5142.673 kb on - strand, within Pf6N2E2_4408 at 5142.709 kb on - strand, within Pf6N2E2_4408 at 5142.717 kb on + strand, within Pf6N2E2_4408 at 5142.717 kb on + strand, within Pf6N2E2_4408 at 5142.717 kb on + strand, within Pf6N2E2_4408 at 5142.718 kb on - strand, within Pf6N2E2_4408 at 5142.795 kb on + strand, within Pf6N2E2_4408 at 5142.795 kb on + strand, within Pf6N2E2_4408 at 5142.795 kb on + strand, within Pf6N2E2_4408 at 5142.796 kb on - strand, within Pf6N2E2_4408 at 5142.796 kb on - strand, within Pf6N2E2_4408 at 5142.796 kb on - strand, within Pf6N2E2_4408 at 5142.796 kb on - strand, within Pf6N2E2_4408 at 5142.840 kb on + strand, within Pf6N2E2_4408 at 5142.840 kb on + strand, within Pf6N2E2_4408 at 5142.840 kb on + strand, within Pf6N2E2_4408 at 5142.840 kb on + strand, within Pf6N2E2_4408 at 5142.840 kb on + strand, within Pf6N2E2_4408 at 5142.840 kb on + strand, within Pf6N2E2_4408 at 5142.840 kb on + strand, within Pf6N2E2_4408 at 5142.841 kb on - strand, within Pf6N2E2_4408 at 5142.841 kb on - strand, within Pf6N2E2_4408 at 5142.841 kb on - strand, within Pf6N2E2_4408 at 5142.904 kb on + strand, within Pf6N2E2_4408 at 5142.904 kb on + strand, within Pf6N2E2_4408 at 5142.904 kb on + strand, within Pf6N2E2_4408 at 5143.024 kb on + strand at 5143.025 kb on - strand at 5143.025 kb on - strand at 5143.083 kb on + strand at 5143.083 kb on + strand at 5143.084 kb on - strand at 5143.241 kb on - strand at 5143.249 kb on + strand at 5143.249 kb on + strand at 5143.249 kb on + strand at 5143.250 kb on - strand at 5143.282 kb on + strand at 5143.573 kb on - strand at 5143.573 kb on - strand at 5143.573 kb on - strand at 5143.615 kb on - strand at 5143.711 kb on + strand, within Pf6N2E2_4409 at 5143.757 kb on + strand, within Pf6N2E2_4409 at 5143.757 kb on + strand, within Pf6N2E2_4409 at 5143.757 kb on + strand, within Pf6N2E2_4409 at 5143.757 kb on + strand, within Pf6N2E2_4409 at 5143.757 kb on + strand, within Pf6N2E2_4409 at 5143.757 kb on + strand, within Pf6N2E2_4409 at 5143.757 kb on + strand, within Pf6N2E2_4409 at 5143.758 kb on - strand, within Pf6N2E2_4409 at 5143.758 kb on - strand, within Pf6N2E2_4409 at 5143.766 kb on + strand, within Pf6N2E2_4409 at 5143.766 kb on + strand, within Pf6N2E2_4409 at 5143.766 kb on + strand, within Pf6N2E2_4409 at 5143.766 kb on + strand, within Pf6N2E2_4409 at 5143.766 kb on + strand, within Pf6N2E2_4409 at 5143.766 kb on + strand, within Pf6N2E2_4409 at 5143.766 kb on + strand, within Pf6N2E2_4409 at 5143.766 kb on + strand, within Pf6N2E2_4409 at 5143.766 kb on + strand, within Pf6N2E2_4409 at 5143.767 kb on - strand, within Pf6N2E2_4409 at 5143.767 kb on - strand, within Pf6N2E2_4409 at 5143.767 kb on - strand, within Pf6N2E2_4409 at 5143.767 kb on - strand, within Pf6N2E2_4409 at 5143.767 kb on - strand, within Pf6N2E2_4409 at 5143.935 kb on - strand, within Pf6N2E2_4409 at 5143.935 kb on - strand, within Pf6N2E2_4409 at 5144.027 kb on + strand, within Pf6N2E2_4409
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 5,140,685 - Pf6N2E2_4406 0.74 -0.2 5,140,685 - Pf6N2E2_4406 0.74 +1.1 5,140,685 - Pf6N2E2_4406 0.74 +1.2 5,140,705 + Pf6N2E2_4406 0.75 -3.4 5,140,705 + Pf6N2E2_4406 0.75 -0.7 5,140,705 + Pf6N2E2_4406 0.75 +2.5 5,140,705 + Pf6N2E2_4406 0.75 -1.0 5,140,705 + Pf6N2E2_4406 0.75 +0.8 5,140,706 - Pf6N2E2_4406 0.75 +1.5 5,140,706 - Pf6N2E2_4406 0.75 +2.0 5,140,823 - Pf6N2E2_4406 0.82 -2.5 5,140,834 + Pf6N2E2_4406 0.83 -0.2 5,140,834 + Pf6N2E2_4406 0.83 -2.1 5,140,835 - +1.4 5,140,861 + Pf6N2E2_4406 0.84 -0.8 5,140,870 + Pf6N2E2_4406 0.85 +1.2 5,140,870 + Pf6N2E2_4406 0.85 -2.9 5,140,870 + Pf6N2E2_4406 0.85 +1.3 5,140,870 + Pf6N2E2_4406 0.85 +0.2 5,140,871 - Pf6N2E2_4406 0.85 +1.6 5,140,871 - Pf6N2E2_4406 0.85 -2.8 5,140,871 - Pf6N2E2_4406 0.85 +2.7 5,140,871 - Pf6N2E2_4406 0.85 +1.5 5,141,098 + +0.9 5,141,098 + -0.5 5,141,098 + -0.4 5,141,098 + +0.1 5,141,099 - +0.8 5,141,099 - -0.6 5,141,099 - -0.5 5,141,114 + -0.4 5,141,114 + +2.5 5,141,115 - -1.1 5,141,115 - +0.9 5,141,115 - -1.0 5,141,115 - -0.4 5,141,172 + Pf6N2E2_4407 0.11 +1.6 5,141,172 + Pf6N2E2_4407 0.11 -1.2 5,141,172 + Pf6N2E2_4407 0.11 +1.4 5,141,208 + Pf6N2E2_4407 0.18 -1.4 5,141,208 + Pf6N2E2_4407 0.18 -2.4 5,141,209 - Pf6N2E2_4407 0.18 -0.4 5,141,209 - Pf6N2E2_4407 0.18 +0.1 5,141,272 - Pf6N2E2_4407 0.29 -3.0 5,141,272 - Pf6N2E2_4407 0.29 -3.0 5,141,337 + Pf6N2E2_4407 0.41 -2.3 5,141,337 + Pf6N2E2_4407 0.41 -0.7 5,141,337 + Pf6N2E2_4407 0.41 -0.2 5,141,338 - Pf6N2E2_4407 0.41 -0.9 5,141,338 - Pf6N2E2_4407 0.41 +0.3 5,141,433 + Pf6N2E2_4407 0.59 -0.1 5,141,437 - Pf6N2E2_4407 0.59 -3.2 5,141,612 - +0.2 5,141,700 + +0.4 5,141,700 + +1.6 5,141,701 - +1.4 5,141,701 - -2.0 5,141,751 + +1.8 5,141,751 + +0.2 5,141,795 + +1.8 5,141,796 - +1.6 5,141,856 + Pf6N2E2_4408 0.14 +1.3 5,141,856 + Pf6N2E2_4408 0.14 -1.3 5,141,856 + Pf6N2E2_4408 0.14 -1.4 5,141,856 + Pf6N2E2_4408 0.14 +2.0 5,141,856 + Pf6N2E2_4408 0.14 +1.2 5,141,857 - Pf6N2E2_4408 0.14 +0.0 5,141,857 - Pf6N2E2_4408 0.14 +2.8 5,141,857 - Pf6N2E2_4408 0.14 +0.9 5,141,857 - Pf6N2E2_4408 0.14 -2.5 5,141,857 - Pf6N2E2_4408 0.14 +0.6 5,141,857 - Pf6N2E2_4408 0.14 -2.4 5,141,857 - Pf6N2E2_4408 0.14 +0.1 5,141,857 - Pf6N2E2_4408 0.14 +2.4 5,141,857 - Pf6N2E2_4408 0.14 +0.9 5,141,857 - Pf6N2E2_4408 0.14 +0.8 5,141,857 - Pf6N2E2_4408 0.14 +1.5 5,141,857 - Pf6N2E2_4408 0.14 +0.7 5,141,857 - Pf6N2E2_4408 0.14 +0.1 5,141,857 - Pf6N2E2_4408 0.14 -2.5 5,141,967 + Pf6N2E2_4408 0.21 -3.1 5,141,968 - Pf6N2E2_4408 0.21 +0.0 5,142,247 - Pf6N2E2_4408 0.41 -0.6 5,142,247 - Pf6N2E2_4408 0.41 -1.6 5,142,247 - Pf6N2E2_4408 0.41 -1.6 5,142,394 - Pf6N2E2_4408 0.51 +2.5 5,142,501 + Pf6N2E2_4408 0.59 -1.0 5,142,502 - Pf6N2E2_4408 0.59 -1.5 5,142,502 - Pf6N2E2_4408 0.59 +0.7 5,142,502 - Pf6N2E2_4408 0.59 +0.0 5,142,666 + Pf6N2E2_4408 0.70 +2.0 5,142,666 + Pf6N2E2_4408 0.70 -2.2 5,142,666 + Pf6N2E2_4408 0.70 -0.9 5,142,667 - Pf6N2E2_4408 0.70 -0.2 5,142,667 - Pf6N2E2_4408 0.70 +1.7 5,142,667 - Pf6N2E2_4408 0.70 +0.6 5,142,667 - Pf6N2E2_4408 0.70 +1.6 5,142,667 - Pf6N2E2_4408 0.70 -1.3 5,142,672 + Pf6N2E2_4408 0.71 +1.5 5,142,673 - Pf6N2E2_4408 0.71 +1.8 5,142,709 - Pf6N2E2_4408 0.73 +2.4 5,142,717 + Pf6N2E2_4408 0.74 -1.0 5,142,717 + Pf6N2E2_4408 0.74 +1.8 5,142,717 + Pf6N2E2_4408 0.74 +0.4 5,142,718 - Pf6N2E2_4408 0.74 +1.9 5,142,795 + Pf6N2E2_4408 0.79 +1.9 5,142,795 + Pf6N2E2_4408 0.79 -0.2 5,142,795 + Pf6N2E2_4408 0.79 +0.4 5,142,796 - Pf6N2E2_4408 0.80 +1.7 5,142,796 - Pf6N2E2_4408 0.80 -2.9 5,142,796 - Pf6N2E2_4408 0.80 -0.6 5,142,796 - Pf6N2E2_4408 0.80 -2.0 5,142,840 + Pf6N2E2_4408 0.83 -2.3 5,142,840 + Pf6N2E2_4408 0.83 +1.6 5,142,840 + Pf6N2E2_4408 0.83 -1.1 5,142,840 + Pf6N2E2_4408 0.83 +0.7 5,142,840 + Pf6N2E2_4408 0.83 +0.6 5,142,840 + Pf6N2E2_4408 0.83 -0.3 5,142,840 + Pf6N2E2_4408 0.83 +0.3 5,142,841 - Pf6N2E2_4408 0.83 -1.7 5,142,841 - Pf6N2E2_4408 0.83 +2.0 5,142,841 - Pf6N2E2_4408 0.83 +1.7 5,142,904 + Pf6N2E2_4408 0.87 -2.0 5,142,904 + Pf6N2E2_4408 0.87 +0.0 5,142,904 + Pf6N2E2_4408 0.87 -1.2 5,143,024 + -3.0 5,143,025 - -2.6 5,143,025 - +1.7 5,143,083 + -0.5 5,143,083 + -0.5 5,143,084 - +1.5 5,143,241 - -1.5 5,143,249 + +1.1 5,143,249 + +1.2 5,143,249 + -1.1 5,143,250 - -0.8 5,143,282 + -1.6 5,143,573 - -1.3 5,143,573 - +0.8 5,143,573 - +0.5 5,143,615 - -0.2 5,143,711 + Pf6N2E2_4409 0.12 +0.3 5,143,757 + Pf6N2E2_4409 0.15 -0.7 5,143,757 + Pf6N2E2_4409 0.15 +2.4 5,143,757 + Pf6N2E2_4409 0.15 -0.7 5,143,757 + Pf6N2E2_4409 0.15 +1.3 5,143,757 + Pf6N2E2_4409 0.15 -2.9 5,143,757 + Pf6N2E2_4409 0.15 +0.2 5,143,757 + Pf6N2E2_4409 0.15 -1.2 5,143,758 - Pf6N2E2_4409 0.15 +1.8 5,143,758 - Pf6N2E2_4409 0.15 -1.8 5,143,766 + Pf6N2E2_4409 0.16 +0.6 5,143,766 + Pf6N2E2_4409 0.16 +0.0 5,143,766 + Pf6N2E2_4409 0.16 -4.0 5,143,766 + Pf6N2E2_4409 0.16 -2.8 5,143,766 + Pf6N2E2_4409 0.16 +0.6 5,143,766 + Pf6N2E2_4409 0.16 +1.8 5,143,766 + Pf6N2E2_4409 0.16 +1.2 5,143,766 + Pf6N2E2_4409 0.16 -0.2 5,143,766 + Pf6N2E2_4409 0.16 +1.2 5,143,767 - Pf6N2E2_4409 0.16 -2.5 5,143,767 - Pf6N2E2_4409 0.16 +0.7 5,143,767 - Pf6N2E2_4409 0.16 +0.8 5,143,767 - Pf6N2E2_4409 0.16 -0.7 5,143,767 - Pf6N2E2_4409 0.16 -1.5 5,143,935 - Pf6N2E2_4409 0.26 -2.4 5,143,935 - Pf6N2E2_4409 0.26 -2.7 5,144,027 + Pf6N2E2_4409 0.31 -0.5
Or see this region's nucleotide sequence