Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4315 and Pf6N2E2_4316 are separated by 84 nucleotides Pf6N2E2_4316 and Pf6N2E2_4317 are separated by 63 nucleotides Pf6N2E2_4317 and Pf6N2E2_4318 are separated by 18 nucleotides
Pf6N2E2_4315 - Rhs-family protein, at 5,033,634 to 5,038,031
_4315
Pf6N2E2_4316 - hypothetical protein, at 5,038,116 to 5,038,466
_4316
Pf6N2E2_4317 - FIG00964469: hypothetical protein, at 5,038,530 to 5,038,964
_4317
Pf6N2E2_4318 - VgrG-3 protein, at 5,038,983 to 5,040,914
_4318
Position (kb)
5038
5039 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5037.595 kb on + strand at 5037.774 kb on + strand at 5037.775 kb on - strand at 5037.951 kb on + strand at 5037.952 kb on - strand at 5037.952 kb on - strand at 5038.131 kb on + strand at 5038.132 kb on - strand at 5038.250 kb on + strand, within Pf6N2E2_4316 at 5038.251 kb on - strand, within Pf6N2E2_4316 at 5038.256 kb on - strand, within Pf6N2E2_4316 at 5038.305 kb on - strand, within Pf6N2E2_4316 at 5038.305 kb on - strand, within Pf6N2E2_4316 at 5038.324 kb on + strand, within Pf6N2E2_4316 at 5038.324 kb on + strand, within Pf6N2E2_4316 at 5038.325 kb on - strand, within Pf6N2E2_4316 at 5038.325 kb on - strand, within Pf6N2E2_4316 at 5038.325 kb on - strand, within Pf6N2E2_4316 at 5038.336 kb on + strand, within Pf6N2E2_4316 at 5038.336 kb on + strand, within Pf6N2E2_4316 at 5038.336 kb on + strand, within Pf6N2E2_4316 at 5038.336 kb on + strand, within Pf6N2E2_4316 at 5038.336 kb on + strand, within Pf6N2E2_4316 at 5038.336 kb on + strand, within Pf6N2E2_4316 at 5038.336 kb on + strand, within Pf6N2E2_4316 at 5038.336 kb on + strand, within Pf6N2E2_4316 at 5038.337 kb on - strand, within Pf6N2E2_4316 at 5038.337 kb on - strand, within Pf6N2E2_4316 at 5038.337 kb on - strand, within Pf6N2E2_4316 at 5038.337 kb on - strand, within Pf6N2E2_4316 at 5038.337 kb on - strand, within Pf6N2E2_4316 at 5038.337 kb on - strand, within Pf6N2E2_4316 at 5038.344 kb on + strand, within Pf6N2E2_4316 at 5038.344 kb on + strand, within Pf6N2E2_4316 at 5038.344 kb on + strand, within Pf6N2E2_4316 at 5038.345 kb on - strand, within Pf6N2E2_4316 at 5038.345 kb on - strand, within Pf6N2E2_4316 at 5038.375 kb on + strand, within Pf6N2E2_4316 at 5038.376 kb on - strand, within Pf6N2E2_4316 at 5038.376 kb on - strand, within Pf6N2E2_4316 at 5038.376 kb on - strand, within Pf6N2E2_4316 at 5038.376 kb on - strand, within Pf6N2E2_4316 at 5038.376 kb on - strand, within Pf6N2E2_4316 at 5038.376 kb on - strand, within Pf6N2E2_4316 at 5038.376 kb on - strand, within Pf6N2E2_4316 at 5038.438 kb on + strand at 5038.438 kb on + strand at 5038.439 kb on - strand at 5038.439 kb on - strand at 5038.615 kb on + strand, within Pf6N2E2_4317 at 5038.615 kb on + strand, within Pf6N2E2_4317 at 5038.615 kb on + strand, within Pf6N2E2_4317 at 5038.615 kb on + strand, within Pf6N2E2_4317 at 5038.666 kb on + strand, within Pf6N2E2_4317 at 5038.958 kb on - strand at 5038.985 kb on - strand at 5039.122 kb on + strand at 5039.227 kb on + strand, within Pf6N2E2_4318 at 5039.227 kb on + strand, within Pf6N2E2_4318 at 5039.227 kb on + strand, within Pf6N2E2_4318 at 5039.227 kb on + strand, within Pf6N2E2_4318 at 5039.228 kb on - strand, within Pf6N2E2_4318 at 5039.228 kb on - strand, within Pf6N2E2_4318 at 5039.228 kb on - strand, within Pf6N2E2_4318
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 5,037,595 + -2.2 5,037,774 + +1.0 5,037,775 - -0.2 5,037,951 + +1.2 5,037,952 - +2.3 5,037,952 - +0.9 5,038,131 + -1.3 5,038,132 - +0.4 5,038,250 + Pf6N2E2_4316 0.38 -2.2 5,038,251 - Pf6N2E2_4316 0.38 +0.6 5,038,256 - Pf6N2E2_4316 0.40 -1.5 5,038,305 - Pf6N2E2_4316 0.54 -1.8 5,038,305 - Pf6N2E2_4316 0.54 -2.1 5,038,324 + Pf6N2E2_4316 0.59 -2.3 5,038,324 + Pf6N2E2_4316 0.59 +2.7 5,038,325 - Pf6N2E2_4316 0.60 -1.9 5,038,325 - Pf6N2E2_4316 0.60 +0.9 5,038,325 - Pf6N2E2_4316 0.60 -1.2 5,038,336 + Pf6N2E2_4316 0.63 -1.2 5,038,336 + Pf6N2E2_4316 0.63 +2.9 5,038,336 + Pf6N2E2_4316 0.63 +1.9 5,038,336 + Pf6N2E2_4316 0.63 -0.7 5,038,336 + Pf6N2E2_4316 0.63 -1.9 5,038,336 + Pf6N2E2_4316 0.63 +0.8 5,038,336 + Pf6N2E2_4316 0.63 +1.9 5,038,336 + Pf6N2E2_4316 0.63 -2.4 5,038,337 - Pf6N2E2_4316 0.63 -1.1 5,038,337 - Pf6N2E2_4316 0.63 +0.2 5,038,337 - Pf6N2E2_4316 0.63 +1.7 5,038,337 - Pf6N2E2_4316 0.63 +1.2 5,038,337 - Pf6N2E2_4316 0.63 +2.6 5,038,337 - Pf6N2E2_4316 0.63 +1.6 5,038,344 + Pf6N2E2_4316 0.65 +2.2 5,038,344 + Pf6N2E2_4316 0.65 -2.1 5,038,344 + Pf6N2E2_4316 0.65 +0.5 5,038,345 - Pf6N2E2_4316 0.65 +0.5 5,038,345 - Pf6N2E2_4316 0.65 -2.7 5,038,375 + Pf6N2E2_4316 0.74 +1.9 5,038,376 - Pf6N2E2_4316 0.74 +0.9 5,038,376 - Pf6N2E2_4316 0.74 -0.8 5,038,376 - Pf6N2E2_4316 0.74 +1.7 5,038,376 - Pf6N2E2_4316 0.74 +0.4 5,038,376 - Pf6N2E2_4316 0.74 +1.9 5,038,376 - Pf6N2E2_4316 0.74 +1.8 5,038,376 - Pf6N2E2_4316 0.74 +1.1 5,038,438 + +1.2 5,038,438 + -2.2 5,038,439 - +0.6 5,038,439 - +2.0 5,038,615 + Pf6N2E2_4317 0.20 +0.7 5,038,615 + Pf6N2E2_4317 0.20 +0.6 5,038,615 + Pf6N2E2_4317 0.20 +0.1 5,038,615 + Pf6N2E2_4317 0.20 -1.5 5,038,666 + Pf6N2E2_4317 0.31 +1.0 5,038,958 - -1.1 5,038,985 - -2.7 5,039,122 + -1.4 5,039,227 + Pf6N2E2_4318 0.13 -2.0 5,039,227 + Pf6N2E2_4318 0.13 +1.0 5,039,227 + Pf6N2E2_4318 0.13 +0.8 5,039,227 + Pf6N2E2_4318 0.13 -2.4 5,039,228 - Pf6N2E2_4318 0.13 +0.7 5,039,228 - Pf6N2E2_4318 0.13 -1.2 5,039,228 - Pf6N2E2_4318 0.13 +1.5
Or see this region's nucleotide sequence