Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4041 and Pf6N2E2_4042 are separated by 319 nucleotides Pf6N2E2_4042 and Pf6N2E2_4043 are separated by 344 nucleotides Pf6N2E2_4043 and Pf6N2E2_4044 are separated by 106 nucleotides Pf6N2E2_4044 and Pf6N2E2_4045 are separated by 27 nucleotides Pf6N2E2_4045 and Pf6N2E2_4046 overlap by 4 nucleotides
Pf6N2E2_4041 - Acetyl-CoA hydrolase (EC 3.1.2.1), at 4,722,622 to 4,724,115
_4041
Pf6N2E2_4042 - FIG00954150: hypothetical protein, at 4,724,435 to 4,724,650
_4042
Pf6N2E2_4043 - Outer membrane receptor proteins, mostly Fe transport, at 4,724,995 to 4,725,312
_4043
Pf6N2E2_4044 - Holliday junction resolvasome, helicase subunit, at 4,725,419 to 4,725,727
_4044
Pf6N2E2_4045 - FIG00953248: hypothetical protein, at 4,725,755 to 4,726,075
_4045
Pf6N2E2_4046 - FIG00953482: hypothetical protein, at 4,726,072 to 4,728,033
_4046
Position (kb)
4724
4725
4726 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4724.008 kb on + strand at 4724.009 kb on - strand at 4724.049 kb on + strand at 4724.085 kb on - strand at 4724.116 kb on + strand at 4724.117 kb on - strand at 4724.142 kb on + strand at 4724.142 kb on + strand at 4724.143 kb on - strand at 4724.143 kb on - strand at 4724.301 kb on - strand at 4724.313 kb on - strand at 4724.313 kb on - strand at 4724.319 kb on + strand at 4724.350 kb on - strand at 4724.354 kb on - strand at 4724.404 kb on + strand at 4724.405 kb on - strand at 4724.443 kb on + strand at 4724.443 kb on + strand at 4724.443 kb on + strand at 4724.443 kb on + strand at 4724.443 kb on + strand at 4724.780 kb on + strand at 4724.781 kb on - strand at 4724.791 kb on + strand at 4724.794 kb on - strand at 4724.812 kb on + strand at 4724.813 kb on - strand at 4724.818 kb on + strand at 4724.819 kb on - strand at 4724.819 kb on - strand at 4724.900 kb on + strand at 4724.900 kb on + strand at 4724.900 kb on + strand at 4724.901 kb on - strand at 4724.901 kb on - strand at 4724.901 kb on - strand at 4724.901 kb on - strand at 4724.904 kb on - strand at 4724.904 kb on - strand at 4724.954 kb on + strand at 4724.955 kb on - strand at 4724.955 kb on - strand at 4724.955 kb on - strand at 4724.955 kb on - strand at 4724.955 kb on - strand at 4724.955 kb on - strand at 4725.241 kb on + strand, within Pf6N2E2_4043 at 4725.241 kb on + strand, within Pf6N2E2_4043 at 4725.241 kb on + strand, within Pf6N2E2_4043 at 4725.241 kb on + strand, within Pf6N2E2_4043 at 4725.242 kb on - strand, within Pf6N2E2_4043 at 4725.242 kb on - strand, within Pf6N2E2_4043 at 4725.242 kb on - strand, within Pf6N2E2_4043 at 4725.242 kb on - strand, within Pf6N2E2_4043 at 4725.459 kb on + strand, within Pf6N2E2_4044 at 4725.460 kb on - strand, within Pf6N2E2_4044 at 4725.527 kb on + strand, within Pf6N2E2_4044 at 4725.565 kb on + strand, within Pf6N2E2_4044 at 4725.565 kb on + strand, within Pf6N2E2_4044 at 4725.565 kb on + strand, within Pf6N2E2_4044 at 4725.566 kb on - strand, within Pf6N2E2_4044 at 4725.656 kb on + strand, within Pf6N2E2_4044 at 4725.725 kb on + strand at 4725.725 kb on + strand at 4725.725 kb on + strand at 4725.725 kb on + strand at 4725.725 kb on + strand at 4725.725 kb on + strand at 4725.725 kb on + strand at 4725.726 kb on - strand at 4725.726 kb on - strand at 4725.726 kb on - strand at 4725.726 kb on - strand at 4725.726 kb on - strand at 4725.726 kb on - strand at 4725.726 kb on - strand at 4725.726 kb on - strand at 4725.726 kb on - strand at 4725.726 kb on - strand at 4725.729 kb on + strand at 4725.730 kb on - strand at 4725.730 kb on - strand at 4725.730 kb on - strand at 4725.730 kb on - strand at 4725.730 kb on - strand at 4725.735 kb on + strand at 4725.735 kb on + strand at 4725.735 kb on + strand at 4725.735 kb on + strand at 4725.736 kb on - strand at 4725.736 kb on - strand at 4725.736 kb on - strand at 4725.736 kb on - strand at 4725.736 kb on - strand at 4725.736 kb on - strand at 4725.736 kb on - strand at 4725.761 kb on - strand at 4725.783 kb on + strand at 4725.820 kb on + strand, within Pf6N2E2_4045 at 4725.820 kb on + strand, within Pf6N2E2_4045 at 4725.820 kb on + strand, within Pf6N2E2_4045 at 4725.821 kb on - strand, within Pf6N2E2_4045 at 4725.821 kb on - strand, within Pf6N2E2_4045 at 4725.821 kb on - strand, within Pf6N2E2_4045 at 4725.839 kb on - strand, within Pf6N2E2_4045 at 4725.839 kb on - strand, within Pf6N2E2_4045
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 4,724,008 + -1.3 4,724,009 - -0.2 4,724,049 + -0.5 4,724,085 - -1.9 4,724,116 + +0.0 4,724,117 - +1.7 4,724,142 + +1.1 4,724,142 + -2.4 4,724,143 - -0.4 4,724,143 - -2.3 4,724,301 - +3.1 4,724,313 - -0.7 4,724,313 - -1.4 4,724,319 + -3.1 4,724,350 - -1.7 4,724,354 - +0.6 4,724,404 + +1.6 4,724,405 - +1.2 4,724,443 + -3.8 4,724,443 + -2.1 4,724,443 + +0.1 4,724,443 + +1.2 4,724,443 + +2.1 4,724,780 + -2.7 4,724,781 - -0.9 4,724,791 + -1.5 4,724,794 - +2.0 4,724,812 + -1.0 4,724,813 - -0.4 4,724,818 + +1.0 4,724,819 - +0.0 4,724,819 - +2.0 4,724,900 + -1.6 4,724,900 + +0.5 4,724,900 + -2.4 4,724,901 - +1.3 4,724,901 - +0.4 4,724,901 - +1.9 4,724,901 - +0.8 4,724,904 - -2.9 4,724,904 - -0.5 4,724,954 + +0.9 4,724,955 - +1.2 4,724,955 - +1.6 4,724,955 - -1.2 4,724,955 - -2.1 4,724,955 - -1.9 4,724,955 - +2.7 4,725,241 + Pf6N2E2_4043 0.77 -1.7 4,725,241 + Pf6N2E2_4043 0.77 +0.2 4,725,241 + Pf6N2E2_4043 0.77 +0.9 4,725,241 + Pf6N2E2_4043 0.77 -2.5 4,725,242 - Pf6N2E2_4043 0.78 -2.4 4,725,242 - Pf6N2E2_4043 0.78 +0.1 4,725,242 - Pf6N2E2_4043 0.78 -3.3 4,725,242 - Pf6N2E2_4043 0.78 -1.6 4,725,459 + Pf6N2E2_4044 0.13 -2.7 4,725,460 - Pf6N2E2_4044 0.13 -1.6 4,725,527 + Pf6N2E2_4044 0.35 -2.4 4,725,565 + Pf6N2E2_4044 0.47 -3.3 4,725,565 + Pf6N2E2_4044 0.47 -0.4 4,725,565 + Pf6N2E2_4044 0.47 -2.9 4,725,566 - Pf6N2E2_4044 0.48 -3.5 4,725,656 + Pf6N2E2_4044 0.77 -3.0 4,725,725 + +2.2 4,725,725 + +1.6 4,725,725 + +1.6 4,725,725 + +1.6 4,725,725 + +0.8 4,725,725 + -0.4 4,725,725 + +2.3 4,725,726 - +1.4 4,725,726 - +2.5 4,725,726 - -2.7 4,725,726 - -2.9 4,725,726 - +0.3 4,725,726 - -1.1 4,725,726 - -3.0 4,725,726 - -1.7 4,725,726 - -1.6 4,725,726 - -1.6 4,725,729 + +2.1 4,725,730 - -1.9 4,725,730 - -0.4 4,725,730 - -0.7 4,725,730 - -1.8 4,725,730 - -0.8 4,725,735 + +0.2 4,725,735 + +1.6 4,725,735 + +1.7 4,725,735 + +0.5 4,725,736 - +0.5 4,725,736 - -0.7 4,725,736 - -2.3 4,725,736 - -2.2 4,725,736 - -3.3 4,725,736 - -1.9 4,725,736 - -1.8 4,725,761 - -1.7 4,725,783 + -1.5 4,725,820 + Pf6N2E2_4045 0.20 -1.1 4,725,820 + Pf6N2E2_4045 0.20 -0.8 4,725,820 + Pf6N2E2_4045 0.20 -0.5 4,725,821 - Pf6N2E2_4045 0.21 -1.3 4,725,821 - Pf6N2E2_4045 0.21 -2.3 4,725,821 - Pf6N2E2_4045 0.21 -1.1 4,725,839 - Pf6N2E2_4045 0.26 -0.0 4,725,839 - Pf6N2E2_4045 0.26 +0.2
Or see this region's nucleotide sequence