Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3891 and Pf6N2E2_3892 are separated by 297 nucleotides Pf6N2E2_3892 and Pf6N2E2_3893 are separated by 78 nucleotides Pf6N2E2_3893 and Pf6N2E2_3894 are separated by 45 nucleotides
Pf6N2E2_3891 - Putative formate dehydrogenase oxidoreductase protein, at 4,522,692 to 4,525,040
_3891
Pf6N2E2_3892 - FIG01270322: hypothetical protein, at 4,525,338 to 4,525,817
_3892
Pf6N2E2_3893 - putative 4-hydroxybenzoyl-CoA thioesterase, at 4,525,896 to 4,526,315
_3893
Pf6N2E2_3894 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 4,526,361 to 4,527,587
_3894
Position (kb)
4525
4526
4527 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4524.906 kb on + strand at 4524.906 kb on + strand at 4524.906 kb on + strand at 4524.906 kb on + strand at 4524.907 kb on - strand at 4524.907 kb on - strand at 4524.907 kb on - strand at 4525.046 kb on + strand at 4525.046 kb on + strand at 4525.046 kb on + strand at 4525.046 kb on + strand at 4525.046 kb on + strand at 4525.046 kb on + strand at 4525.046 kb on + strand at 4525.047 kb on - strand at 4525.047 kb on - strand at 4525.133 kb on + strand at 4525.133 kb on + strand at 4525.134 kb on - strand at 4525.134 kb on - strand at 4525.190 kb on - strand at 4525.279 kb on + strand at 4525.284 kb on + strand at 4525.285 kb on - strand at 4525.403 kb on + strand, within Pf6N2E2_3892 at 4525.491 kb on - strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.713 kb on + strand, within Pf6N2E2_3892 at 4525.714 kb on - strand, within Pf6N2E2_3892 at 4525.714 kb on - strand, within Pf6N2E2_3892 at 4525.740 kb on + strand, within Pf6N2E2_3892 at 4525.743 kb on + strand, within Pf6N2E2_3892 at 4525.743 kb on + strand, within Pf6N2E2_3892 at 4525.744 kb on - strand, within Pf6N2E2_3892 at 4525.803 kb on + strand at 4525.803 kb on + strand at 4525.807 kb on - strand at 4525.829 kb on + strand at 4525.926 kb on + strand at 4525.927 kb on - strand at 4525.974 kb on + strand, within Pf6N2E2_3893 at 4525.974 kb on + strand, within Pf6N2E2_3893 at 4525.975 kb on - strand, within Pf6N2E2_3893 at 4525.975 kb on - strand, within Pf6N2E2_3893 at 4525.975 kb on - strand, within Pf6N2E2_3893 at 4525.975 kb on - strand, within Pf6N2E2_3893 at 4526.039 kb on - strand, within Pf6N2E2_3893 at 4526.116 kb on + strand, within Pf6N2E2_3893 at 4526.116 kb on + strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.191 kb on + strand, within Pf6N2E2_3893 at 4526.191 kb on + strand, within Pf6N2E2_3893 at 4526.191 kb on + strand, within Pf6N2E2_3893 at 4526.191 kb on + strand, within Pf6N2E2_3893 at 4526.191 kb on + strand, within Pf6N2E2_3893 at 4526.192 kb on - strand, within Pf6N2E2_3893 at 4526.192 kb on - strand, within Pf6N2E2_3893 at 4526.192 kb on - strand, within Pf6N2E2_3893 at 4526.192 kb on - strand, within Pf6N2E2_3893 at 4526.192 kb on - strand, within Pf6N2E2_3893 at 4526.192 kb on - strand, within Pf6N2E2_3893 at 4526.192 kb on - strand, within Pf6N2E2_3893 at 4526.195 kb on + strand, within Pf6N2E2_3893 at 4526.195 kb on + strand, within Pf6N2E2_3893 at 4526.196 kb on - strand, within Pf6N2E2_3893 at 4526.196 kb on - strand, within Pf6N2E2_3893 at 4526.196 kb on - strand, within Pf6N2E2_3893 at 4526.222 kb on - strand, within Pf6N2E2_3893 at 4526.222 kb on - strand, within Pf6N2E2_3893 at 4526.222 kb on - strand, within Pf6N2E2_3893 at 4526.224 kb on + strand, within Pf6N2E2_3893 at 4526.224 kb on + strand, within Pf6N2E2_3893 at 4526.224 kb on + strand, within Pf6N2E2_3893 at 4526.224 kb on + strand, within Pf6N2E2_3893 at 4526.224 kb on + strand, within Pf6N2E2_3893 at 4526.224 kb on + strand, within Pf6N2E2_3893 at 4526.224 kb on + strand, within Pf6N2E2_3893 at 4526.225 kb on - strand, within Pf6N2E2_3893 at 4526.225 kb on - strand, within Pf6N2E2_3893 at 4526.225 kb on - strand, within Pf6N2E2_3893 at 4526.225 kb on - strand, within Pf6N2E2_3893 at 4526.248 kb on + strand, within Pf6N2E2_3893 at 4526.248 kb on + strand, within Pf6N2E2_3893 at 4526.249 kb on - strand, within Pf6N2E2_3893 at 4526.277 kb on - strand at 4526.277 kb on - strand at 4526.670 kb on + strand, within Pf6N2E2_3894 at 4526.743 kb on + strand, within Pf6N2E2_3894 at 4526.744 kb on - strand, within Pf6N2E2_3894 at 4526.749 kb on + strand, within Pf6N2E2_3894 at 4526.749 kb on + strand, within Pf6N2E2_3894 at 4526.749 kb on + strand, within Pf6N2E2_3894 at 4526.749 kb on + strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.806 kb on - strand, within Pf6N2E2_3894 at 4526.884 kb on + strand, within Pf6N2E2_3894 at 4526.885 kb on - strand, within Pf6N2E2_3894 at 4526.986 kb on + strand, within Pf6N2E2_3894 at 4526.986 kb on + strand, within Pf6N2E2_3894 at 4526.986 kb on + strand, within Pf6N2E2_3894 at 4526.986 kb on + strand, within Pf6N2E2_3894 at 4526.986 kb on + strand, within Pf6N2E2_3894 at 4526.987 kb on - strand, within Pf6N2E2_3894 at 4526.987 kb on - strand, within Pf6N2E2_3894 at 4526.987 kb on - strand, within Pf6N2E2_3894 at 4526.987 kb on - strand, within Pf6N2E2_3894 at 4526.987 kb on - strand, within Pf6N2E2_3894 at 4526.987 kb on - strand, within Pf6N2E2_3894 at 4526.987 kb on - strand, within Pf6N2E2_3894
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 4,524,906 + +2.1 4,524,906 + +1.7 4,524,906 + +0.1 4,524,906 + -2.1 4,524,907 - +0.3 4,524,907 - +0.9 4,524,907 - +0.1 4,525,046 + +2.0 4,525,046 + -1.1 4,525,046 + +2.2 4,525,046 + -3.0 4,525,046 + +1.5 4,525,046 + -0.4 4,525,046 + -1.0 4,525,047 - +0.7 4,525,047 - -2.3 4,525,133 + +1.2 4,525,133 + +0.2 4,525,134 - +1.8 4,525,134 - +1.6 4,525,190 - -0.2 4,525,279 + +1.0 4,525,284 + -0.7 4,525,285 - +1.0 4,525,403 + Pf6N2E2_3892 0.14 -0.9 4,525,491 - Pf6N2E2_3892 0.32 +1.5 4,525,680 + Pf6N2E2_3892 0.71 -0.1 4,525,680 + Pf6N2E2_3892 0.71 +1.7 4,525,680 + Pf6N2E2_3892 0.71 +0.8 4,525,680 + Pf6N2E2_3892 0.71 +0.9 4,525,680 + Pf6N2E2_3892 0.71 +2.1 4,525,680 + Pf6N2E2_3892 0.71 -2.9 4,525,680 + Pf6N2E2_3892 0.71 +0.9 4,525,680 + Pf6N2E2_3892 0.71 +0.7 4,525,680 + Pf6N2E2_3892 0.71 +2.3 4,525,680 + Pf6N2E2_3892 0.71 +0.2 4,525,680 + Pf6N2E2_3892 0.71 -1.1 4,525,680 + Pf6N2E2_3892 0.71 +1.7 4,525,680 + Pf6N2E2_3892 0.71 +1.1 4,525,680 + Pf6N2E2_3892 0.71 +1.2 4,525,680 + Pf6N2E2_3892 0.71 -0.1 4,525,680 + Pf6N2E2_3892 0.71 +2.5 4,525,680 + Pf6N2E2_3892 0.71 -2.0 4,525,680 + Pf6N2E2_3892 0.71 -3.0 4,525,681 - Pf6N2E2_3892 0.71 -1.0 4,525,681 - Pf6N2E2_3892 0.71 -0.0 4,525,681 - Pf6N2E2_3892 0.71 -0.9 4,525,681 - Pf6N2E2_3892 0.71 -1.3 4,525,681 - Pf6N2E2_3892 0.71 -2.2 4,525,681 - Pf6N2E2_3892 0.71 -0.9 4,525,681 - Pf6N2E2_3892 0.71 -0.2 4,525,681 - Pf6N2E2_3892 0.71 -1.5 4,525,681 - Pf6N2E2_3892 0.71 +0.9 4,525,681 - Pf6N2E2_3892 0.71 +0.5 4,525,713 + Pf6N2E2_3892 0.78 -0.3 4,525,714 - Pf6N2E2_3892 0.78 +1.5 4,525,714 - Pf6N2E2_3892 0.78 -2.2 4,525,740 + Pf6N2E2_3892 0.84 +0.2 4,525,743 + Pf6N2E2_3892 0.84 -0.1 4,525,743 + Pf6N2E2_3892 0.84 +1.8 4,525,744 - Pf6N2E2_3892 0.85 -0.9 4,525,803 + +2.4 4,525,803 + -1.1 4,525,807 - +1.9 4,525,829 + -0.0 4,525,926 + -0.6 4,525,927 - -0.6 4,525,974 + Pf6N2E2_3893 0.19 +1.2 4,525,974 + Pf6N2E2_3893 0.19 -2.1 4,525,975 - Pf6N2E2_3893 0.19 +1.4 4,525,975 - Pf6N2E2_3893 0.19 -2.2 4,525,975 - Pf6N2E2_3893 0.19 -2.7 4,525,975 - Pf6N2E2_3893 0.19 +0.3 4,526,039 - Pf6N2E2_3893 0.34 -0.2 4,526,116 + Pf6N2E2_3893 0.52 -1.9 4,526,116 + Pf6N2E2_3893 0.52 -0.7 4,526,117 - Pf6N2E2_3893 0.53 -3.0 4,526,117 - Pf6N2E2_3893 0.53 +1.2 4,526,117 - Pf6N2E2_3893 0.53 +0.3 4,526,117 - Pf6N2E2_3893 0.53 +1.6 4,526,117 - Pf6N2E2_3893 0.53 -0.6 4,526,117 - Pf6N2E2_3893 0.53 +1.6 4,526,117 - Pf6N2E2_3893 0.53 +1.0 4,526,117 - Pf6N2E2_3893 0.53 -4.2 4,526,117 - Pf6N2E2_3893 0.53 +0.2 4,526,191 + Pf6N2E2_3893 0.70 -2.9 4,526,191 + Pf6N2E2_3893 0.70 -1.3 4,526,191 + Pf6N2E2_3893 0.70 +1.7 4,526,191 + Pf6N2E2_3893 0.70 +1.4 4,526,191 + Pf6N2E2_3893 0.70 +0.4 4,526,192 - Pf6N2E2_3893 0.70 +1.1 4,526,192 - Pf6N2E2_3893 0.70 +0.5 4,526,192 - Pf6N2E2_3893 0.70 +2.1 4,526,192 - Pf6N2E2_3893 0.70 +0.3 4,526,192 - Pf6N2E2_3893 0.70 +0.9 4,526,192 - Pf6N2E2_3893 0.70 -2.2 4,526,192 - Pf6N2E2_3893 0.70 +1.2 4,526,195 + Pf6N2E2_3893 0.71 -1.3 4,526,195 + Pf6N2E2_3893 0.71 -1.7 4,526,196 - Pf6N2E2_3893 0.71 +1.6 4,526,196 - Pf6N2E2_3893 0.71 -0.5 4,526,196 - Pf6N2E2_3893 0.71 -2.8 4,526,222 - Pf6N2E2_3893 0.78 +1.6 4,526,222 - Pf6N2E2_3893 0.78 +1.7 4,526,222 - Pf6N2E2_3893 0.78 +1.6 4,526,224 + Pf6N2E2_3893 0.78 -0.7 4,526,224 + Pf6N2E2_3893 0.78 -1.6 4,526,224 + Pf6N2E2_3893 0.78 +0.4 4,526,224 + Pf6N2E2_3893 0.78 +1.1 4,526,224 + Pf6N2E2_3893 0.78 -2.4 4,526,224 + Pf6N2E2_3893 0.78 -2.9 4,526,224 + Pf6N2E2_3893 0.78 +2.5 4,526,225 - Pf6N2E2_3893 0.78 +2.6 4,526,225 - Pf6N2E2_3893 0.78 -3.4 4,526,225 - Pf6N2E2_3893 0.78 -2.0 4,526,225 - Pf6N2E2_3893 0.78 -0.5 4,526,248 + Pf6N2E2_3893 0.84 +1.8 4,526,248 + Pf6N2E2_3893 0.84 -2.2 4,526,249 - Pf6N2E2_3893 0.84 +1.1 4,526,277 - +0.6 4,526,277 - -2.9 4,526,670 + Pf6N2E2_3894 0.25 -0.6 4,526,743 + Pf6N2E2_3894 0.31 -2.5 4,526,744 - Pf6N2E2_3894 0.31 -2.5 4,526,749 + Pf6N2E2_3894 0.32 -2.4 4,526,749 + Pf6N2E2_3894 0.32 -1.1 4,526,749 + Pf6N2E2_3894 0.32 -0.1 4,526,749 + Pf6N2E2_3894 0.32 -1.2 4,526,750 - Pf6N2E2_3894 0.32 -1.9 4,526,750 - Pf6N2E2_3894 0.32 -1.6 4,526,750 - Pf6N2E2_3894 0.32 -1.9 4,526,750 - Pf6N2E2_3894 0.32 -0.7 4,526,750 - Pf6N2E2_3894 0.32 +2.7 4,526,750 - Pf6N2E2_3894 0.32 -0.2 4,526,806 - Pf6N2E2_3894 0.36 +0.9 4,526,884 + Pf6N2E2_3894 0.43 +1.6 4,526,885 - Pf6N2E2_3894 0.43 -1.5 4,526,986 + Pf6N2E2_3894 0.51 -0.9 4,526,986 + Pf6N2E2_3894 0.51 -2.7 4,526,986 + Pf6N2E2_3894 0.51 +0.9 4,526,986 + Pf6N2E2_3894 0.51 -2.5 4,526,986 + Pf6N2E2_3894 0.51 +1.8 4,526,987 - Pf6N2E2_3894 0.51 -0.2 4,526,987 - Pf6N2E2_3894 0.51 +1.1 4,526,987 - Pf6N2E2_3894 0.51 -1.8 4,526,987 - Pf6N2E2_3894 0.51 +1.2 4,526,987 - Pf6N2E2_3894 0.51 +1.1 4,526,987 - Pf6N2E2_3894 0.51 -3.0 4,526,987 - Pf6N2E2_3894 0.51 +1.0
Or see this region's nucleotide sequence