Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_373 and Pf6N2E2_374 are separated by 87 nucleotides Pf6N2E2_374 and Pf6N2E2_375 are separated by 45 nucleotides Pf6N2E2_375 and Pf6N2E2_376 are separated by 128 nucleotides
Pf6N2E2_373 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 440,796 to 442,088
_373
Pf6N2E2_374 - FIG00953249: hypothetical protein, at 442,176 to 442,832
_374
Pf6N2E2_375 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 442,878 to 443,366
_375
Pf6N2E2_376 - Similar to non-heme chloroperoxidase, sll5080 homolog, at 443,495 to 444,448
_376
Position (kb) 442
443 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 441.270 kb on + strand, within Pf6N2E2_373 at 441.271 kb on - strand, within Pf6N2E2_373 at 441.330 kb on + strand, within Pf6N2E2_373 at 441.330 kb on + strand, within Pf6N2E2_373 at 441.699 kb on + strand, within Pf6N2E2_373 at 441.700 kb on - strand, within Pf6N2E2_373 at 442.133 kb on + strand at 442.327 kb on + strand, within Pf6N2E2_374 at 442.328 kb on - strand, within Pf6N2E2_374 at 442.341 kb on + strand, within Pf6N2E2_374 at 442.503 kb on + strand, within Pf6N2E2_374 at 442.503 kb on + strand, within Pf6N2E2_374 at 442.503 kb on + strand, within Pf6N2E2_374 at 442.504 kb on - strand, within Pf6N2E2_374 at 442.774 kb on - strand at 442.774 kb on - strand at 442.839 kb on + strand at 442.853 kb on - strand at 442.853 kb on - strand at 443.043 kb on + strand, within Pf6N2E2_375 at 443.043 kb on + strand, within Pf6N2E2_375 at 443.170 kb on + strand, within Pf6N2E2_375 at 443.171 kb on - strand, within Pf6N2E2_375 at 443.205 kb on + strand, within Pf6N2E2_375 at 443.292 kb on + strand, within Pf6N2E2_375 at 443.301 kb on + strand, within Pf6N2E2_375 at 443.301 kb on + strand, within Pf6N2E2_375 at 443.497 kb on - strand at 443.497 kb on - strand at 443.736 kb on + strand, within Pf6N2E2_376 at 443.737 kb on - strand, within Pf6N2E2_376 at 443.740 kb on + strand, within Pf6N2E2_376 at 443.740 kb on + strand, within Pf6N2E2_376 at 443.740 kb on + strand, within Pf6N2E2_376 at 443.741 kb on - strand, within Pf6N2E2_376 at 443.741 kb on - strand, within Pf6N2E2_376 at 443.741 kb on - strand, within Pf6N2E2_376 at 443.820 kb on + strand, within Pf6N2E2_376 at 443.820 kb on + strand, within Pf6N2E2_376
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 441,270 + Pf6N2E2_373 0.37 -0.7 441,271 - Pf6N2E2_373 0.37 -2.6 441,330 + Pf6N2E2_373 0.41 -2.2 441,330 + Pf6N2E2_373 0.41 +2.0 441,699 + Pf6N2E2_373 0.70 -1.6 441,700 - Pf6N2E2_373 0.70 -1.1 442,133 + -0.9 442,327 + Pf6N2E2_374 0.23 -3.6 442,328 - Pf6N2E2_374 0.23 +1.7 442,341 + Pf6N2E2_374 0.25 -1.1 442,503 + Pf6N2E2_374 0.50 +0.4 442,503 + Pf6N2E2_374 0.50 -1.3 442,503 + Pf6N2E2_374 0.50 -2.0 442,504 - Pf6N2E2_374 0.50 +0.3 442,774 - +5.6 442,774 - +1.3 442,839 + -0.3 442,853 - +1.4 442,853 - -0.7 443,043 + Pf6N2E2_375 0.34 +0.6 443,043 + Pf6N2E2_375 0.34 -1.3 443,170 + Pf6N2E2_375 0.60 -1.2 443,171 - Pf6N2E2_375 0.60 -2.7 443,205 + Pf6N2E2_375 0.67 -1.0 443,292 + Pf6N2E2_375 0.85 -1.7 443,301 + Pf6N2E2_375 0.87 -1.0 443,301 + Pf6N2E2_375 0.87 -0.2 443,497 - -1.1 443,497 - -0.9 443,736 + Pf6N2E2_376 0.25 +1.9 443,737 - Pf6N2E2_376 0.25 -1.6 443,740 + Pf6N2E2_376 0.26 -1.3 443,740 + Pf6N2E2_376 0.26 +0.7 443,740 + Pf6N2E2_376 0.26 +1.5 443,741 - Pf6N2E2_376 0.26 -1.3 443,741 - Pf6N2E2_376 0.26 -0.8 443,741 - Pf6N2E2_376 0.26 +1.0 443,820 + Pf6N2E2_376 0.34 +0.0 443,820 + Pf6N2E2_376 0.34 -4.0
Or see this region's nucleotide sequence