Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_352 and Pf6N2E2_353 are separated by 166 nucleotides Pf6N2E2_353 and Pf6N2E2_354 overlap by 11 nucleotides Pf6N2E2_354 and Pf6N2E2_355 are separated by 152 nucleotides
Pf6N2E2_352 - Opine oxidase subunit B, at 417,890 to 419,071
_352
Pf6N2E2_353 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 419,238 to 419,642
_353
Pf6N2E2_354 - Type III PLP / low-specificity D-threonine aldolase, at 419,632 to 420,711
_354
Pf6N2E2_355 - Transcriptional regulator, LysR family, at 420,864 to 421,748
_355
Position (kb) 419
420
421 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 418.653 kb on + strand, within Pf6N2E2_352 at 418.654 kb on - strand, within Pf6N2E2_352 at 418.870 kb on - strand, within Pf6N2E2_352 at 418.870 kb on - strand, within Pf6N2E2_352 at 418.948 kb on - strand, within Pf6N2E2_352 at 419.048 kb on + strand at 419.048 kb on + strand at 419.048 kb on + strand at 419.048 kb on + strand at 419.049 kb on - strand at 419.049 kb on - strand at 419.049 kb on - strand at 419.075 kb on - strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.239 kb on + strand at 419.239 kb on + strand at 419.240 kb on - strand at 419.291 kb on - strand, within Pf6N2E2_353 at 419.291 kb on - strand, within Pf6N2E2_353 at 419.351 kb on - strand, within Pf6N2E2_353 at 419.359 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.551 kb on + strand, within Pf6N2E2_353 at 419.551 kb on + strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.584 kb on + strand, within Pf6N2E2_353 at 419.585 kb on - strand, within Pf6N2E2_353 at 419.691 kb on - strand at 419.691 kb on - strand at 419.691 kb on - strand at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354 at 420.242 kb on + strand, within Pf6N2E2_354 at 420.242 kb on + strand, within Pf6N2E2_354 at 420.243 kb on - strand, within Pf6N2E2_354 at 420.521 kb on + strand, within Pf6N2E2_354 at 420.522 kb on - strand, within Pf6N2E2_354 at 420.522 kb on - strand at 420.700 kb on - strand at 420.721 kb on - strand at 420.804 kb on + strand at 420.804 kb on + strand at 420.805 kb on - strand at 421.225 kb on + strand, within Pf6N2E2_355 at 421.225 kb on + strand, within Pf6N2E2_355 at 421.225 kb on + strand, within Pf6N2E2_355 at 421.225 kb on + strand, within Pf6N2E2_355 at 421.225 kb on + strand, within Pf6N2E2_355 at 421.226 kb on - strand, within Pf6N2E2_355 at 421.226 kb on - strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.428 kb on - strand, within Pf6N2E2_355 at 421.428 kb on - strand, within Pf6N2E2_355
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 418,653 + Pf6N2E2_352 0.65 +0.2 418,654 - Pf6N2E2_352 0.65 +0.5 418,870 - Pf6N2E2_352 0.83 -1.9 418,870 - Pf6N2E2_352 0.83 +0.2 418,948 - Pf6N2E2_352 0.90 -2.8 419,048 + +0.7 419,048 + -1.5 419,048 + +2.2 419,048 + -1.8 419,049 - +1.0 419,049 - +1.4 419,049 - -2.5 419,075 - -0.1 419,112 + -2.4 419,112 + -1.8 419,112 + -0.8 419,112 + -2.3 419,112 + +1.0 419,112 + -0.9 419,112 + -1.8 419,112 + +1.7 419,112 + -3.0 419,113 - -1.0 419,113 - +1.2 419,113 - -0.9 419,113 - +0.7 419,113 - +0.9 419,113 - -1.3 419,239 + +1.4 419,239 + +2.2 419,240 - +0.7 419,291 - Pf6N2E2_353 0.13 -2.3 419,291 - Pf6N2E2_353 0.13 +1.8 419,351 - Pf6N2E2_353 0.28 -1.2 419,359 + Pf6N2E2_353 0.30 -0.0 419,401 + Pf6N2E2_353 0.40 +0.2 419,401 + Pf6N2E2_353 0.40 -0.6 419,401 + Pf6N2E2_353 0.40 +2.0 419,401 + Pf6N2E2_353 0.40 -1.0 419,401 + Pf6N2E2_353 0.40 +2.0 419,402 - Pf6N2E2_353 0.40 +0.3 419,402 - Pf6N2E2_353 0.40 -3.0 419,402 - Pf6N2E2_353 0.40 -1.6 419,402 - Pf6N2E2_353 0.40 -3.0 419,551 + Pf6N2E2_353 0.77 +2.4 419,551 + Pf6N2E2_353 0.77 -2.6 419,552 - Pf6N2E2_353 0.78 +0.2 419,552 - Pf6N2E2_353 0.78 -0.1 419,552 - Pf6N2E2_353 0.78 +0.2 419,552 - Pf6N2E2_353 0.78 +1.6 419,584 + Pf6N2E2_353 0.85 +1.4 419,585 - Pf6N2E2_353 0.86 -0.6 419,691 - -2.0 419,691 - -2.3 419,691 - -1.8 420,059 + Pf6N2E2_354 0.40 -1.5 420,059 + Pf6N2E2_354 0.40 +0.9 420,059 + Pf6N2E2_354 0.40 +0.9 420,059 + Pf6N2E2_354 0.40 -2.3 420,059 + Pf6N2E2_354 0.40 -1.3 420,060 - Pf6N2E2_354 0.40 +1.7 420,060 - Pf6N2E2_354 0.40 +2.4 420,060 - Pf6N2E2_354 0.40 +1.8 420,242 + Pf6N2E2_354 0.56 +0.9 420,242 + Pf6N2E2_354 0.56 -2.1 420,243 - Pf6N2E2_354 0.57 +2.1 420,521 + Pf6N2E2_354 0.82 +0.8 420,522 - Pf6N2E2_354 0.82 +1.1 420,522 - -0.3 420,700 - +1.9 420,721 - +1.7 420,804 + +2.0 420,804 + -2.1 420,805 - -1.4 421,225 + Pf6N2E2_355 0.41 +1.1 421,225 + Pf6N2E2_355 0.41 -0.3 421,225 + Pf6N2E2_355 0.41 -0.6 421,225 + Pf6N2E2_355 0.41 -1.0 421,225 + Pf6N2E2_355 0.41 -2.6 421,226 - Pf6N2E2_355 0.41 +3.6 421,226 - Pf6N2E2_355 0.41 +1.1 421,427 + Pf6N2E2_355 0.64 +1.7 421,427 + Pf6N2E2_355 0.64 +2.7 421,427 + Pf6N2E2_355 0.64 +0.5 421,427 + Pf6N2E2_355 0.64 -1.9 421,427 + Pf6N2E2_355 0.64 +0.1 421,428 - Pf6N2E2_355 0.64 +2.5 421,428 - Pf6N2E2_355 0.64 -1.0
Or see this region's nucleotide sequence