Experiment: deoxycholate 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3223 and Pf6N2E2_3224 are separated by 129 nucleotides Pf6N2E2_3224 and Pf6N2E2_3225 are separated by 181 nucleotides Pf6N2E2_3225 and Pf6N2E2_3226 are separated by 102 nucleotides Pf6N2E2_3226 and Pf6N2E2_3227 are separated by 96 nucleotides
Pf6N2E2_3223 - uncharacterized protein SCO0976, at 3,728,874 to 3,729,506
_3223
Pf6N2E2_3224 - Lactoylglutathione lyase and related lyases, at 3,729,636 to 3,730,052
_3224
Pf6N2E2_3225 - hypothetical protein, at 3,730,234 to 3,731,283
_3225
Pf6N2E2_3226 - Predicted D-glucarate or D-galactorate regulator, GntR family, at 3,731,386 to 3,732,099
_3226
Pf6N2E2_3227 - Predicted D-glucarate or D-galactorate regulator, GntR family, at 3,732,196 to 3,732,939
_3227
Position (kb)
3730
3731
3732 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3729.302 kb on + strand, within Pf6N2E2_3223 at 3729.303 kb on - strand, within Pf6N2E2_3223 at 3729.330 kb on + strand, within Pf6N2E2_3223 at 3729.331 kb on - strand, within Pf6N2E2_3223 at 3729.331 kb on - strand, within Pf6N2E2_3223 at 3729.338 kb on - strand, within Pf6N2E2_3223 at 3729.507 kb on - strand at 3729.531 kb on + strand at 3729.531 kb on + strand at 3729.531 kb on + strand at 3729.532 kb on - strand at 3729.532 kb on - strand at 3729.532 kb on - strand at 3729.603 kb on + strand at 3729.604 kb on - strand at 3729.635 kb on + strand at 3729.659 kb on + strand at 3729.660 kb on - strand at 3729.660 kb on - strand at 3729.683 kb on - strand, within Pf6N2E2_3224 at 3729.712 kb on - strand, within Pf6N2E2_3224 at 3729.712 kb on - strand, within Pf6N2E2_3224 at 3729.713 kb on + strand, within Pf6N2E2_3224 at 3729.713 kb on + strand, within Pf6N2E2_3224 at 3729.714 kb on - strand, within Pf6N2E2_3224 at 3729.993 kb on + strand, within Pf6N2E2_3224 at 3730.103 kb on - strand at 3730.289 kb on + strand at 3730.290 kb on - strand at 3730.290 kb on - strand at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.444 kb on - strand, within Pf6N2E2_3225 at 3730.444 kb on - strand, within Pf6N2E2_3225 at 3730.444 kb on - strand, within Pf6N2E2_3225 at 3730.624 kb on + strand, within Pf6N2E2_3225 at 3730.880 kb on - strand, within Pf6N2E2_3225 at 3730.951 kb on + strand, within Pf6N2E2_3225 at 3731.161 kb on + strand, within Pf6N2E2_3225 at 3731.161 kb on + strand, within Pf6N2E2_3225 at 3731.162 kb on - strand, within Pf6N2E2_3225 at 3731.199 kb on - strand at 3731.246 kb on + strand at 3731.247 kb on - strand at 3731.281 kb on + strand at 3731.282 kb on - strand at 3731.430 kb on + strand at 3731.430 kb on + strand at 3731.430 kb on + strand at 3731.431 kb on - strand at 3731.431 kb on - strand at 3731.431 kb on - strand at 3731.495 kb on + strand, within Pf6N2E2_3226 at 3731.495 kb on + strand, within Pf6N2E2_3226 at 3731.580 kb on + strand, within Pf6N2E2_3226 at 3731.580 kb on + strand, within Pf6N2E2_3226 at 3731.581 kb on - strand, within Pf6N2E2_3226 at 3731.581 kb on - strand, within Pf6N2E2_3226 at 3732.028 kb on + strand at 3732.029 kb on - strand at 3732.029 kb on - strand at 3732.106 kb on + strand at 3732.107 kb on - strand at 3732.113 kb on - strand at 3732.134 kb on + strand at 3732.134 kb on + strand at 3732.135 kb on - strand at 3732.136 kb on + strand at 3732.163 kb on + strand at 3732.163 kb on + strand at 3732.163 kb on + strand at 3732.164 kb on - strand at 3732.164 kb on - strand at 3732.164 kb on - strand at 3732.164 kb on - strand at 3732.165 kb on + strand at 3732.165 kb on + strand at 3732.165 kb on + strand at 3732.166 kb on - strand at 3732.166 kb on - strand at 3732.212 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction deoxycholate 0.0125 M remove 3,729,302 + Pf6N2E2_3223 0.68 +1.6 3,729,303 - Pf6N2E2_3223 0.68 -2.6 3,729,330 + Pf6N2E2_3223 0.72 +1.1 3,729,331 - Pf6N2E2_3223 0.72 +0.4 3,729,331 - Pf6N2E2_3223 0.72 -2.7 3,729,338 - Pf6N2E2_3223 0.73 +0.2 3,729,507 - -1.5 3,729,531 + +1.4 3,729,531 + -0.8 3,729,531 + -0.6 3,729,532 - -2.5 3,729,532 - +1.6 3,729,532 - +0.2 3,729,603 + -0.1 3,729,604 - +2.5 3,729,635 + -3.1 3,729,659 + -0.2 3,729,660 - +1.8 3,729,660 - -1.4 3,729,683 - Pf6N2E2_3224 0.11 +1.2 3,729,712 - Pf6N2E2_3224 0.18 -2.5 3,729,712 - Pf6N2E2_3224 0.18 +0.4 3,729,713 + Pf6N2E2_3224 0.18 +1.3 3,729,713 + Pf6N2E2_3224 0.18 -1.1 3,729,714 - Pf6N2E2_3224 0.19 +0.6 3,729,993 + Pf6N2E2_3224 0.86 -3.4 3,730,103 - -0.4 3,730,289 + -1.4 3,730,290 - -2.4 3,730,290 - -1.6 3,730,441 - Pf6N2E2_3225 0.20 -0.7 3,730,441 - Pf6N2E2_3225 0.20 -0.3 3,730,441 - Pf6N2E2_3225 0.20 +0.2 3,730,441 - Pf6N2E2_3225 0.20 +1.6 3,730,441 - Pf6N2E2_3225 0.20 -2.1 3,730,443 + Pf6N2E2_3225 0.20 +1.0 3,730,443 + Pf6N2E2_3225 0.20 -2.2 3,730,443 + Pf6N2E2_3225 0.20 +1.4 3,730,443 + Pf6N2E2_3225 0.20 +1.9 3,730,443 + Pf6N2E2_3225 0.20 -1.1 3,730,443 + Pf6N2E2_3225 0.20 -1.2 3,730,444 - Pf6N2E2_3225 0.20 +1.4 3,730,444 - Pf6N2E2_3225 0.20 +0.4 3,730,444 - Pf6N2E2_3225 0.20 -0.3 3,730,624 + Pf6N2E2_3225 0.37 +2.3 3,730,880 - Pf6N2E2_3225 0.62 +2.6 3,730,951 + Pf6N2E2_3225 0.68 +1.6 3,731,161 + Pf6N2E2_3225 0.88 +0.5 3,731,161 + Pf6N2E2_3225 0.88 +0.0 3,731,162 - Pf6N2E2_3225 0.88 +0.7 3,731,199 - -1.0 3,731,246 + +1.2 3,731,247 - +1.2 3,731,281 + -1.6 3,731,282 - -0.6 3,731,430 + -1.7 3,731,430 + +1.8 3,731,430 + -1.5 3,731,431 - -1.9 3,731,431 - -1.1 3,731,431 - +0.4 3,731,495 + Pf6N2E2_3226 0.15 +1.6 3,731,495 + Pf6N2E2_3226 0.15 -1.4 3,731,580 + Pf6N2E2_3226 0.27 +1.7 3,731,580 + Pf6N2E2_3226 0.27 -1.0 3,731,581 - Pf6N2E2_3226 0.27 +1.3 3,731,581 - Pf6N2E2_3226 0.27 -0.4 3,732,028 + -1.0 3,732,029 - +1.2 3,732,029 - +1.0 3,732,106 + +1.0 3,732,107 - -0.7 3,732,113 - +2.3 3,732,134 + -1.3 3,732,134 + -2.1 3,732,135 - +1.7 3,732,136 + +0.4 3,732,163 + +0.7 3,732,163 + +0.0 3,732,163 + -0.8 3,732,164 - -2.0 3,732,164 - -0.3 3,732,164 - +0.4 3,732,164 - -2.2 3,732,165 + -3.2 3,732,165 + +1.4 3,732,165 + +2.6 3,732,166 - -1.8 3,732,166 - +1.8 3,732,212 + -0.8
Or see this region's nucleotide sequence